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As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP+Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组,以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组,以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组,作用时间为48小时,以不加药物干预作为对照组,Hoechst33258染色荧光显微镜下观察细胞形态的变化,流式细胞仪检测细胞凋亡峰;紫外光分光光度计检测线粒体PTP开放情况,流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化,Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性,γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

Circulating antigen was detected in 29 out of 70 cases with paragonimiasis with a sensitivity of 41.5%. The rate of cross reaction in cases with clonorchiasis sinensis and schistosomiasis was 25%(5/20) and 20%(4/20), respectively, and it was negative in 60 casess with other parasitic infections and healthy subjects, with an overall specificity of 93.6%.

用CAg-dot-ELISA检测70份卫氏并殖吸虫病临床诊断患者血清,阳性29份,敏感性为41.5%(29/70),与华支睾吸虫病和日本血吸虫病患者血清分别有25%(5/20)和20%(4/20)的交叉反应,与其他寄生虫感染者血清和健康人血清(60份)均为阴性,特异性为93.6%。

objective to evaluate effect of lulongshengjing pill on the treatment of oligospermatisma with insuffi-ciency of kidney.methods 100cases with oligospermatism with insufficiency of kidney were treated by lulongshengjing pill.the therapeutic course was three months.testicular fluid routine was carried on every month.changes of testicular flu-id indexes(includes amount,colliquation time,spermatozoa density and motility rate)and procreation were observed before and after treatment.results the total effective rate was91%;cure of56;significant improvement of19;improvement of16and ineffective of9.testicular fluid indexes were significantly improved after treatment compared with those before treatment(p.01).conclusion lulongshengjing pill was effective on the treatment of oligospermatism with insufficiency of kidney.

目的 观察鹿茸生精丸治疗肾阳虚型少精子症的临床疗效。方法采用鹿茸生精丸治疗肾阳虚型少精子症患者100例,3个月1个疗程,治疗2个疗程,每月查1次精液常规,观察治疗前后精液参数(精液量、液化时间、精子密度、活率及精子形态)的变化及生育情况。结果治愈56例,显效19例,好转16例,无效9例,总有效率91%。治疗前后精液量、精子密度、活率等项指标均有明显改善,与治疗前比较差异有统计学意义(p.01)。结论鹿茸生精丸治疗肾阳虚型少精子症具有良好疗效。

Result of thermogravity analysis showed that the thermal stability of polyvinylamine chloride lowers with increasing in amination degree. Moreover, properties of dilute aqueous solutions of three types of poly chloride with different amination degree were compared by use of viscosimetric, conductometric, and potentiometric measurements. Results showed that both the electrostatic interaction of neighboring groups on the chain and the reduced viscosity of poly chloride are increased with increasing the degree of amination of poly . The equivalent conductivity is decreased with the increasing the concentration of poly chloride and increased with the increasing the amination degree of poly .

研究了聚乙烯胺盐酸盐的热稳定性和水溶液性质,发现:随着胺化度的提高,聚乙烯胺盐酸盐的对热稳定性逐步降低;聚乙烯胺盐酸盐是一种典型的聚电解质,其水溶液的pH值随平均分子量的变化并不明显,而随着聚乙烯胺胺化度的升高,pH值降低;随着聚乙烯胺盐酸盐的平均聚合度由82.3增加到201.8,它的m值(用于表征聚电解质侧链基团之间相互作用大小的常数)从5.13升高到5.58,而当聚乙烯胺盐酸盐的平均聚合度增加到311.4时,它的m值反而降低到了5.48;随着胺化度由55.65%升高到82.36%,m值由3.65提高到5.50;随着聚乙烯胺盐酸盐的平均聚合度的提高,聚乙烯胺盐酸盐的平均解离常数由7.48降低到7.26,同时随着胺化度的提高,聚乙烯胺盐酸盐的平均解离常数也由8.33降低到7.37;随着聚乙烯胺盐酸盐浓度的提高,溶液的摩尔电导率逐步降低,随着聚乙烯胺胺化度和聚合度的提高,溶液的摩尔电导率有所升高;在pH值为4左右时,聚乙烯胺盐酸盐的比浓粘度分别达到极值,而与聚合度的大小关系不大;聚乙烯胺盐酸盐的特性粘数明显高于其原料聚丙烯酰胺的特性粘数,分子量对特性粘数的影响十分明显,胺化度对特性粘数的影响并不显著。

Methods: the 165 patients with the coprostasis about patient with psychosis were divided into a group with fanxieye and 163 patients with same symptomwere divided into b group with phenolphthalein, 5-10g fanxieye or 0.3g phenolphthalein , po, st.

方法将伴有便秘症状328例精神病人随机分为a(165例)、b(163例)两组,a组给番泻叶5-10g冲水顿服;b组给果导片0.3顿服。

METHODS: From June 2007 to March 2009, 8 patients with terrible triad of the elbow were treated. The fracture of the radial head was classified according to the system of Mason, with typeⅠ in 1 patients, type Ⅱ in 6 and type Ⅲ in 1; fracture of the coronoid process was classified according to the system of Regan and Morrey, with type Ⅰin 2 patients, type Ⅱ in 5, and type Ⅲ in 1. The hollow lag screws and Herbert screws were used to fixe coronoid process and radial head fractures, and repair Annular ligament. Lateral collateral ligament was repaired in 1 case. All injured extremities were treated with a posterior plaster splint with the elbow joint held in 90° of flexion and the forearm in neutral rotation for 2-3 weeks followed by elbow rehabilitation training.

于2007-06/2009-03共收治肘关节恐怖三联征患者8例,其中尺骨冠突骨折按 Regan-Morrey法分类:Ⅰ型2例,Ⅱ型5例,Ⅲ型1例;桡骨头骨折按Mason法分类:Ⅰ型1例,Ⅱ型6例,Ⅲ型1例。8例患者均采取了内固定治疗,采用中空拉力螺钉或Herbert螺钉固定冠突和桡骨头骨折,修补环状韧带,1例缝合修复肘外侧副韧带,术后屈肘90°旋前中立位,以塑形良好的高分子管型石膏固定两三周后开始屈伸和旋转康复训练。

The condensation of aromatic aldehydes with active methylene compounds like malononitrile, cyanoacetamide and ethyl cyanoacetate has been found to proceed very efficiently at room temperature giving excellent yields, but the condensation of heteroaromatic,α,β-unsaturated and aliphatic aldehydes with active methylene compounds proceeded smoothly in 50~65℃ with good yields and the reactions of aromatic, aliphatic ketones as well as p-phthalaldehyde with malononitrile also proceeded in 75~85℃ with moderate yields.

芳香醛与活泼亚甲基化合物如丙二腈、氰基乙酸乙酯、氰基乙酰胺的缩合在室温下即能顺利进行,获得了很高的收率;而对于杂环芳醛、α,β-不饱和的醛以及脂肪醛与活泼亚甲基化合物的缩合,需在50~65℃下进行,也取得了很高的收率;对于脂肪酮、芳香酮以及二元醛与丙二腈的缩合在75~85℃下也能顺利地进行,获得中等以上的收率。

Results The patients aged 13 - 30 including 2 cases of type Ⅰ,7 cases of type Ⅱ and 1 case of type Ⅲ,had frequent symptoms of dysmenorrheal and abdominal pain (7 cases), vaginal puruloid secretion (3 cases) and infertility (3 cases); 10 of them were complicated with double uterus,2 with unicornous uterus, 3 with endometrial cystis; 8 of them were complicated with ipsilatera renal agenesis, 2 with horseshoe kidney; 2 of them were misdiagnosised.

结果患者年龄13-30岁,Ⅰ型2例,Ⅱ型7例,Ⅲ型1例;临床表现为下腹痛和痛经7例,阴道流脓3例,不孕症3例;10例例均合并双子宫,单角子宫2例,内膜囊肿3例;8例合并同侧肾脏缺如,2例合并马蹄肾;误诊2例;治疗以斜膈切开术为主,部分病例同时行内膜囊肿剥除和单角子宫切除。

objective: to investigate diagnosis and treatment choice of detrusor hypoactivity with impaired contractile function on voiding function in benign prostatic hyperplasia.methods:the clinical date of 48 bph patients with dhic were analyzed,including clinical diagnosis,urodynamic parameters,treatment and follow-ups.results:37 patients with detrusor instability and over 40 cmh2o bladder pressure were treated successfully by turp.the time of indwelling suprapubic punctural cystostomy cathter was(1~8)weeks.11cases without di and under 40 cmh2o bladder pressure were treated by suprapubic punctural cystostomy,accepted the test of urodynamics after(1~3)months.7 cases with di were treated successfully by turp,4 cases without di were indwelled suprapubic punctural cystostomy cathter all life.conclusion:dhic worsens the voiding function of the patients with bph,and the test of urodynamic is helpful to diagnose and could provide more convinced evidences for treatment of patients.

分析膀胱过度充盈所致逼尿肌损伤,引起膀胱逼尿肌收缩无力良性前列腺增生48例患者的临床资料,包括诊断,尿动力学检查,治疗及随访结果。结果:37例经尿流动力学检查,诱发存在逼尿肌不稳定,伴膀胱内压达40 cmh2o以上,采取turp,术后留置膀胱造瘘管(1~8)周后全部拔除,排尿通畅。11膀胱内压始终未达到40 cmh2o,且在膀胱注水充盈过程中无逼尿肌不稳定出现,行膀胱造瘘后出院,(1~3)个月后经尿动力学检查复查膀胱逼尿肌收缩力,其中7例膀胱逼尿肌收缩功能有恢复,行经尿道前列腺汽化电切术治愈;剩余4例膀胱逼尿肌收缩功能几乎无任何改善。长期留置膀胱造瘘治疗。结论:良性前列腺增生导致膀胱过度充盈所致逼尿肌损伤,引起膀胱逼尿肌收缩无力患者,术前应用尿流动力学检查对膀胱逼尿肌损伤程度进行认真分析,可进行有针对性的治疗。

Six weeks after drug intervention,we found,in ADM+Res group,the mean tumor volume of MCF-7/ADM cells implanted tumor was 1.203±0.338 cm~3 with statistical significance to compare with other groups(P<0.05),decreased about 50.58%compared with the mean tumor volume of ADM alone group1.979±0.307 cm~3the result of the mean tumor weigh was alike to the mean tumor volume; compared with the control,Res could relieve weigh loss(P<0.05,and the toxicity of treatment was not obvious;Microscopic examination showed that there was massive area of cytoclasis and apoptosis in the tumor tissue of the combination group comparing with other groups.

实验结果提示用药6W后,ADM+Res组移植瘤体积(1.203±0.338)cm~3明显小于其余各组,抑瘤率为50.58%,与单用ADM组(1.979±0.307)cm~3相比有差异P<0.05肿瘤重量的结果与瘤体结果类似;未观察到用药后的毒副作用;经不同处理后,单独注射Res组小鼠体重较生理盐水组及ADM组有所增加(P<0.05,而ADM+Res组治疗前后体重无差异P>0.05HE结果显示与其他组相比,ADM+Res组肿瘤组织中出现大片坏死区域。

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推荐网络例句

The X-ray diffract was used to analyze the crystal structure of the composite. Results show that after the talc was treated with blend coupling agent, the crystal structure of talc changed, and effect Intercalation phenomenon.

通过X射线衍射分析可知,用混合偶联剂对滑石粉进行预处理后,滑石粉晶体结构发生改变,产生了插层现象,使PP/滑石粉复合材料为纳米复合材料。

An authentic Maui wedding might require the services of a 'kahu', which is a Hawaiian minister.

毛伊可能需要一个真实婚礼服务'kahu',这是夏威夷部长。

Funds involving such cases are limited and there is no huge capital flight.

与此有关的资金数额是相对少数的,并没有引发资本外逃现象。