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The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

And see no end to the landscape,new objects presenting themselves as we advan ce;so,in the commencement of life,we set no bounds to our inclinations.nor to the unrestricted opportunities of grastifying them.we have as yet found no obs tacle,no disposition to flag;and it seems that we can go on so forever.we look round in a new world,full of life,and motion,and ceaseless progress;and feel in ourselves all the vigour and spirit to keep pace with it,and do not foresee from any present symptoms how we shall be left behind in the natural course o f things,decline into old age,and drop into the grave.it is the simplicity,and as it were abstractedness of our feelings in youth,thatidentifie s us with nature ,and(our experience being slight and our passions strong)delu des us into a belief of being immortal like it.our short-lives connexion with existence we fondly flatter ourselves,is an indissoluble and lasting union-a h oneymoon that knows neither coldness,jar,nor separation.as infants smile and s leep,we are rocked in the cradle of our wayward fancies,and lulled into securi ty by the roar of the universe around us0we quaff the cup of life with eager h aste without draining it,instead of which it only overflows the more-objects p ress around us,filling the mind with their magnitude and with the strong of de sires that wait upon them,so that we have no room for the thoughts of death.

此时，但觉好风光应接不暇，而且，前程更有美不胜收的新鲜景致。在这生活的开端，我们听任自己的志趣驰骋，放手给它们一切满足的机会。到此为止，我们还没有碰上过什么障碍，也没有感觉到什么疲惫，因此觉得还可以一直这样向前走去，直到永远。我们看到四周一派新天地——生机盎然，变动不居，日新月异；我们觉得自己活力充盈，精神饱满，可与宇宙并驾齐驱。而且，眼前也无任何迹象可以证明，在大自然的发展过程中，我们自己也会落伍，衰老，进入坟墓。由于年轻人天真单纯，可以说是茫然无知，因而将自己跟大自然划上等号；并且，由于经验少而感情盛，误以为自己也能和大自然一样永世长存。我们一厢情愿，痴心妄想，竟把自己在世上的暂时栖身，当作千古不变、万事长存的结合，好像没有冷淡、争执、离别的密月。像婴儿带着微笑入睡，我们躺在用自己编织成的摇篮里，让大千世界的万籁之声催哄我们安然入梦；我们急切切，兴冲冲地畅饮生命之杯，怎么也不会饮干，反而好像永远是满满欲溢；森罗万象纷至沓来，各种欲望随之而生，使我们腾不出工夫想死亡。

ASpheric FeNi nanoparticles with average diameter of about 70 nm were obtained in the system of cetyltrimethyl ammonium bromide/H_2O,while most of the products were nanofilms/rods with the addition of cyclohexane; nearly monodisperse FeNi nanoparticles with average diameter of about 25 nm were prepared in the system of PEG/cyclohexane/H_2O.With the assistance of ultrasonic,a no-surfactant method to 8.5 nm FeCo nanocrystals with high Ms(＞200.5 emu/g)has been developed with economical FeCl_3·6H_2O as iron sources.

a以水合肼为还原剂在十六烷基三甲基溴化铵/H_2O体系中得到了平均粒径约70 nm的FeNi合金球形颗粒，当在此体系中加入环己烷时样品主要形态为纳米膜/棒；于PEG/环己烷/H_2O体系中制备了单分散性较好的平均粒径约25 nm的FeNi合金纳米颗粒，合金纳米颗粒粒径随金属离子浓度减小而减小。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

In the chimney stood a pair of firedogs of iron, ornamented above with two garlanded vases, and flutings which had formerly been silvered with silver leaf, which was a sort of episcopal luxury; above the chimney-piece hung a crucifix of copper, with the silver worn off, fixed on a background of threadbare velvet in a wooden frame from which the gilding had fallen; near the glass door a large table with an inkstand, loaded with a confusion of papers and with huge volumes; before the table an arm-chair of straw; in front of the bed a prie-Dieu, borrowed from the oratory.

在床里的阴暗处，帷的后面，还摆着梳妆用具，残留着他旧时在繁华社会中做人的那些漂亮习气；两扇门，一扇靠近壁炉，通经堂，一扇靠近书橱，通餐室；那书橱是一个大玻璃橱，装满了书；壁炉的木框，描上了仿大理石的花纹，炉里通常是没有火的；壁炉里有一对铁炉篦，篦的两端装饰着两个瓶，瓶上绕着花串和槽形直条花纹，并贴过银箔，那是主教等级的一种奢侈品；上面，在通常挂镜子的地方，有一个银色已褪的铜十字架，钉在一块破旧的黑线上，装在一个金色暗敝的木框里。

In the chimney stood a pair of firedogs of iron, ornamented above with two garlanded vases, and flutings which had formerly been silvered with silver leaf, which was a sort of episcopal luxury; above the chimney-piece hung a crucifix of copper, with the silver worn off, fixed on a background of threadbare velvet in a wooden frame from which the gilding had fallen; near the glass door a large table with an inkstand, loaded with a confusion of papers and with huge volumes; before the table an arm-chair of straw; in front of the bed a prie-Dieu, borrowed from the oratory.

窗门旁边，有一张大桌子，摆了一个墨水瓶，桌上堆着零乱的纸张和大本的书籍。桌子前面，一张麦秸椅。床的前面，一张从经堂里搬来的祈祷椅。

This is the sound of throwing pennies on the ground Turn it up I don't give if they don't hear it enough Bet one Jordans and a dome chambers livin' it up Up top at a high top Famed with the high tops Watching my highlight tick from my sky rocks Ortonville, Michigan, home with a palace The place on the map where I zig and beat Magic Throwing L Balls, had to rip my horn habits With the eight nine bad boy hammer with my cap And you know Chuck did it Not the girls get where they tell 'em Kiss it off the glass Pass it Kick it Call the shots from the bench And finger pointing and pics I'm Coach Chuck Daily AKA Daddy Rich I met Chuck English to flicker what a flicks Ball, net, finger roll Allan Houston off the BIC Yeahh, the BIC with a little with the kicks Kid Snoopy came to who do the Cool Kidz Tickets in the city when we in around town Next door neighbors telling us that is too loud Now, neighbor can you please just turn that mess down?

这是健全的投掷硬币在地面上把它上升我不给，如果他们听不够投注一个约旦和圆顶商会生存它涨顶端高顶端著名的高毛条看着我的突出剔岩石从我的天空 Ortonville ，密歇根州，与宫家地点在地图上，我锯齿和击败魔术投掷L高尔夫球，不得不瑞普我国非洲之角习惯与8个坏男孩与我的第锤而且你知道查没有不是女孩让他们告诉'时间吻把玻璃传递踢它呼叫杆从替补席和手指指向和图片我教练查克每日抗体爸爸富我会见了查英语译成闪烁什么片球网，手指轧辊休斯顿关闭的BIC Yeahh ，百慕大独立委员会，以小的球儿童史努比来谁做酷Kidz 门票在城市周围，当我们在镇隔壁邻居告诉我们，是太大声现在，邻居可以请您谈谈这只是混乱呢？

With this in mind, the researchers then reanalysed data from knowingly HIV-positive heterosexual subjects in Uganda and Zambia who had HIV-negative sexual partners. Those with a viral load of one million particles per millilitre were more than ten times more likely to accidentally infect their partners each year than those with a load of one thousand.But when the researchers calculated the transmission potential over the entire predicted asymptomatic period, those with viral loads between ten thousand and a hundred thousand copies per millilitre of blood were the most likely to infect their partners. HIV-positive subjects with viral counts of 50,000 infected roughly twice as many people as those with counts of one million.

伦敦帝国学院流行病学家Christophe Fraser和他的同事对收集于1982年到1993年之间的100多例HIV阳性的男性同性恋者的数据进行分析，并结合分析来自乌干达和赞比亚的已知有HIV阴性性伴侣的HIV阳性异性恋受调查者的资料，他们发现中度病毒负荷而无症状的HIV携带者更易播散HIV，这是因为中度病毒负荷的HIV携带者的无症状期较长，而那些高病毒负荷而无症状的HIV携带者虽然传染性稍强，但其传染的持续时间却很短，并且在未经治疗的HIV携带者个体中中度病毒负荷是最常见的人群。

Results Histopathological examination showed that 8 patients had cervical intra-epithelial neoplasia,including 4 patients with CIN grade Ⅰ,3 patients with CIN grade Ⅱ,1 patients with grade Ⅲ and 1 patients with carcinoma in situ of cervix;1 patients with invasive carcinoma;113 patients with chronic cervicitis;80 patients had polypi;15 patients had HPV infection.

结果 病理确诊为宫颈不典型增生8例，其中CINⅠ4例，CINⅡ 3例，CINⅢ 1例；宫颈原位癌1例、宫颈浸润癌1例、慢性宫颈炎113例、宫颈息肉80例及HPV感染者15例。

The research on the tissue culture and cell suspension culture showed that thesuitable culture medium for inducing bud was MS supplemented with 1.5mg/L 6-BAand 0.1mg/L NAA, and for the generation-continuing multiplication was MSsupplemented with 1.5mg/L 6-BA and 0.2mg/L NAA. The rooting medium was1/2MS supplemented with 0.5mg/L IBA and the rooting rate was 45.0%. Plantletsurvival after transfer to sand was 52.5%.The induction rate of calli was66.7%～86.6% and the optimum medium was MS medium with 0.5mg/L 6-BA and2.0mg/L 2,4-D. The calli became smallest partical size, friable and had gooddispersion ability after 3 times successive transfer culture, the optimum medium wasMS medium with 0.2mg/L 6-BA and 2.0mg/L 2,4-D. Culturing these particles on sixkinds of MS liquid media with different hormone contents, the optimum medium wasselected basing on he change of the density of single-cell, the density of cellaggrefate and the mass of cell.

对蒜头果进行的组织培养与细胞悬浮培养研究结果表明：MS+6-BA1.5mg／L+NAA0.1mg／L+蔗糖3％激素组合能够较好地诱导芽的初始分化和增殖，适宜的芽苗继代增殖培养基为MS+6-BA1.5mg／L+NAA0.2mg／L+蔗糖3％；采用1／2MS+IBA0.5mg／L+蔗糖3％为生根培养基，生根率为45.0％；移栽到河沙的生根苗成活率为52.5％；愈伤组织诱导率为66.7％～86.6％，其中以MS+6-BA0.5mg／L+2，4-D2.0mg／L+蔗糖3％的培养基最佳，其诱导出的愈伤组织具有较强的增殖能力和较好的脆散结构，最佳继代培养基为MS+6-BA0.2mg／L+2，4-D2.0mg／L+蔗糖3％，且培养基中的6-BA与2，4-D浓度的比值越小，愈伤组织生长越快，结构越脆散，增殖率越高；将继代后的愈伤组织转入6种含不同激素浓度组合的MS液体培养基中进行振荡培养，在综合分析各培养基的单细胞密度，细胞团块密度，细胞生物量增长率等指标后，初步筛选出MS+6-BA0.2mg／L+2，4-D2.0mg／L培养基为较好的液体培养基。

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说，如果是/否的问题回答f可看的价值观念在许多最有限坐标，然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明：基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小，它既能去除奇异点，又能保持图像中目标的边缘不变性，具有很好的滤波效果。