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The opium exudes pacifies with the opium itselffunction related, sells the opium rich profit with the kind related, with leader of laws"s ruling idearelated, with Suiyuan area unique geographical environment, special management system related,was in flood with at that time the opium China"s macroenvironment to have, the modern opiumexudes pacifies concerns with the imperialist nation carrying out invasion of China strategy.

鸦片泛绥与鸦片本身的作用有关，与种贩鸦片丰厚的利润有关，与执政者的执政理念有关，与绥远地区独特的地理环境，特殊的管理体制有关，与当时鸦片泛滥中国的大环境有，近代鸦片泛绥与帝国主义国家推行侵华策略有关。

Penances ought to be joined with the idea of labour, not with that of idleness; with the idea of good, not with that of supereminence; with the idea of frugality, not with that of avarice.

忏悔应当与劳动的观念相结合，而不应该与闲散的观念结合；应当与良善的观念结合，而不应当与出类拔萃的观念结合；应当与节俭的观念结合，而不应当与贪婪的观念结合。

Other characters making regular appearances are Grampa Simpson, Homer's cranky father with his fantastically unbelievable tales of yesteryear and inclination to fall asleep at any given moment; Homer's college friend Barney Gumble, the still single, overweight, beer swilling, constantly burping barfly; Mo, the caustic, self-loathing bar-owner with the secret soft heart; Monty Burns, Homer's boss and the ancient, evil owner of the nuclear power plant; Chief Wiggum, Springfield's junk-food and donut-munching incompetent arm of the law; Apu Nahasapeemapetalon, the oft-held-up manager of the local 'Kwik-e-Mart' store; Krusty the Klown, popular with the kids but offstage a jaded, cynical hack with a long history of overindulgence, gambling and substance abuse; Kent Brockman, the spoiled, self-important TV news anchorman; Troy Mclure, the ageing Hollywood star with the faltering career and bizarre sexual fetish; Rainer Wolfcastle, the Scwarzenegger style action movie hero with the Germanic accent and ultra-right wing views; the list goes on and on

其他经常亮相的角色还有：荷马的古怪父亲——祖父辛普森，他常常会讲一些让人云里雾里、难以置信的陈年故事，而且随时随地都会睡着；至今仍然单身的荷马大学时的朋友——巴尼·甘博，他体重超常，饮酒无度，是一个经常打着饱嗝的酒鬼；酒吧老板——莫，他刻薄且自我憎恨，但却有一颗不为人所知的柔软心肠；荷马的上司——蒙提·彭斯，他是核电厂&老掉牙&的邪恶老板。斯普林菲尔德的警长——威格，他执法不力，还总是使劲儿嚼着垃圾食品和油炸面包圈；经常遭受抢劫的当地便利店的经理——那哈萨彼马彼塔隆；深受孩子们欢迎的小丑克拉斯提，但私下里他却是毫无激情、愤世嫉俗的碌碌无为之人，长期以来放纵无度、赌博并吸毒；被宠坏了的电视新闻主播——肯特·布鲁克曼，他总是那么自命不凡；风华已逝的好莱坞明星——特洛伊·麦克鲁，他的事业摇摇欲坠并有怪异的性癖好；施瓦辛格式的动作电影英雄——瑞纳·沃尔夫卡斯提，他操着一口德国口音，持极右翼观点。角色可真是数也数不清啊

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

This study describes the multi-facet systematic evolution of the said rural community from 1907 to 2007: from the perspective of ethnic culture, it has changed from a single ethnic cultural community of Yoghur(Ethnic Yugur\'s self-name) ethnic group into a multi-ethnic cultural community with Yoghur ethnic group as the dominating majority and embracing the ethnic groups of Han, Tibetan, Tu, Mongolian and Kazak; from the perspective of language, it has changed from a community where Yoghur and Tibetan languages dominated with the local Chinese dialect and the written Chinese language as the subordinate into one where the local Chinese dialect and the written Chinese language dominate with the Yoghur language as the subordinate; in the perspective of marriage and heirship system, from a parallel operation of two systems (patrilineal heirship for official marriages and matrilineal heirship system for matrimonial forms through the head-wearing ceremony) to monogamy and patrilineal heirship; in the perspective of means of living, from animal husbandry complemented with hand knitting and camel caravan transporting to agricultural farming complemented with animal husbandry and many others; in the perspective of social organization, from a traditional tribe to a modern country society; in the perspective of political system and social management, from the customs-authority management which combined the dominating headman hereditary system under the tribal union of an imperial dynasty and the subordinating elite influence, to the resource-interest management of party-government departmental system under the ethnic minority autonomy of a nation state; in the perspective of religious faith, from a coexistence of the Gelug Tibetan Buddhism and Shamanism and the devout ancestor worship to the currently prevalent ancestor worship with religions declining and faith weakening; in the perspective of education, from the local-knowledge-oriented education to an official-knowledge-oriented education, in which modern schooling has become the major educational organization instead of the former traditional temple education; the school has replaced the temple as the "knowledge-culture" communication center; at least in the minds of the local people the central role of family education during the children\'s cultural development has also been replaced by schooling.

本研究描述了1907～2007年间，这个乡村社区的多面向的系统变迁：从族群文化的角度而言，从一个以尧乎尔为绝对主体的单一族群文化社区转变为一个以尧乎尔为主体的、有汉、藏、土、蒙古、哈萨克等组成的多元族群文化社区；从语言文字的角度而言，从一个以尧乎尔语和藏文为主，以汉语方言和汉文为辅的社区转变为一个以汉语方言和汉文为主，以尧乎尔语为辅的社区；从婚姻与继嗣制度的角度而言，从一个双系并行(明媒正娶婚行父系继嗣制度，帐房戴头婚行母系继嗣制度)的社区转变为行一夫一妻制和父系继嗣制度的社区；从生计方式的角度看，从一个以畜牧业生产为主，以手工编织和驼队运输为辅的社区转变为一个以农业为主，以牧业为辅的、多种生计方式并存的社区；从社会组织的形态而言，从一个传统部落社会转变为一个现代乡村社会；从政治制度和社会控制的角度而言，从帝制王朝的部落联盟制下头目世袭制为主，户族精英为辅的习俗—权威型控制方式转变为一个民族-国家中民族区域自治制度下以党政科层制的资源—利益型控制方式；从宗教信仰的角度而言，从一个格鲁派藏传佛教和萨满教并存，祖先崇拜之风甚烈的社区转变为寺庙衰落、信仰淡化、惟祖先崇拜仍广为流行的社区；从教育的角度而言，从一个以地方知识教育为主的社区转变为一个以官方知识教育为主的社区，现代学校教育取代了传统寺庙教育成为社区的主要教育组织形式，学校取代寺庙成为&知识—文化&传播的中心，在青少年儿童的文化成长过程中，家庭教养扮演的中心角色，至少在乡民的观念上被学校教育替代。

The results of cold-flow model test indicate:(1) the pressure drop rises with increasing air quantity and solids quantity respectively, however, much more air quantity and solids quantity minish in the same height position;(2) the pressure drop rises with increasing solids quantity;(3) the average granule concentration minishes with increasing air quantity, and rises with increasing the granule quantity in the same level position;(4) the inhomogeneity of distribution granule concentration minishes with increasing air quantity, and rises with increasing the granule quantity in the same level position.

结果表明：(1)在同一高度截面上，循环流化床反应器炉膛内压降随风量的增加而增加，但是风量过于增大反而使压降在同一截面上减小；(2)压降随初始物料量的增加而增加；(3)在同一水平截面上，颗粒浓度随风量的增大而逐渐减小，随物料量的增大而增大；(4)颗粒浓度分布的不均匀性随风量的增大逐渐减小，随物料量的增加不断增大。

The automatic dryer are expected, or auto-transport function, can automatically feed-share level even after drying into the box; box multi-mobile network with stainless steel materials can automatically flip, hot Bottom-up flow through the network with its network with the top layer of material, heat and mass transfer uniform, full, high-productivity, product quality, good-looking color, with flow-through dryer equipped with cooling and vibration levels expected to lose Device, a low-temperature materials is conducive to packing in a timely manner; chain network dryer with the door activities, to facilitate the clean-up day

该烤箱设有自动均料、自动升运功能，可把料物自动摊平后均匀的送入烘干箱内；箱内采用多层移动式不锈钢网带可使料物自动翻转，热气流自下而上穿过网带及其网带上面的物料层，热质交换均匀、充分，生产效率高，产品质量好，色泽好看，烘干烤箱设有冷却层和震动输料装置，出料温度低，有利于及时包装；烘干烤箱设有活动门，便于每天清理

As with many other weapons and tactics, it could be greatly intensified with quality, and indeed with Cavalry-spirit commanders like Guderian and Rommel, with highly trained or combat experienced soldiers, and with excellent weapons as the Panzer IV and the Stuka dive bomber, the Germans were able to achieve amazing victories with Blitzkrieg.

也正是因为有了拥有骑士精神的指挥官像古德里安和隆美尔，有了经过严格训练拥有战斗经验的士兵，有了出色的武器如四号坦克和斯图卡俯冲轰炸机，德国才能够通过闪电战取得惊人的胜利。

objective to establish immunological methods specific for detecting antigens in different groups of monoclonal antibodies.methods indirect immnofluorescence assay was applied to identify specificity of the two groups of monoclonal antibodies prepared with crude antigen and recombinant antigen of aspergillus fumigatus,respectively.two different double monoclonal antibody sandwich elisa assays established with the two groups of antibodies were performed to detect antigents in the cell culture supermatants of 19 common species of aspergillus,penicillium marneffei,and 5 species of candidas.results the results of indirect immnofluorescence assay indicated that the monoclonal antibodies prepared with crude antigen of aspergillus fumigatus were specific for antigens in both clinical isolates and environmental isolates of aspergillus, whereas the other group of monoclonal antibodies was proved to be specific for aspergillus fumigatus of both clinical and environmental isolates.the elisa assay established with the crude antigen-specfic monoclonal antibodies could detect both of the clinical and environmental isolates of aspergllius, while the other assay could only detect aspergillus fumigatus of both clinical and environmental isolates.and no cross reaction with the cell culture of penialllium marneffei and candidas was observed with the two methods.conclusion the elisa assays can detect both of the clinical and environmental isolates of aspergillus,and differentiate aspergillus fumigatus from other species of aspergillus.

目的 用2组曲霉单克隆抗体建立特异性识别不同种类曲霉抗原的检测方法。方法采用天然烟曲霉抗原免疫，获得广谱针对曲霉抗原的单克隆抗体；采用重组烟曲霉抗原获得特异性针对烟曲霉抗原的单克隆抗体，用间接免疫荧光鉴定，并分别建立2种双抗体夹心elisa法，对19种常见的环境和临床分离曲霉株、马尔尼菲氏青霉菌及念珠菌培养液进行检测。结果间接免疫荧光显示，用天然烟曲霉抗原免疫获得的单克隆抗体(mabs-1)可广谱识别多种曲霉分离株，而重组烟曲霉抗原获得的单克降抗体(mabs-2)仅能特异性结合临床和环境分离的烟曲霉抗原。用mabs-1建立的双抗体夹心elisa法可检测19种常见曲霉株培养液；用特异性针对烟曲霉抗原单克降抗体(mabs-2)建立的双抗体夹心elisa法可特异性检测临床和环境分离株烟曲霉培养液；与其他曲霉株无交叉反应；2种双抗体夹心elisa法与马尔尼菲氏青霉菌及念珠菌培养液均无交叉反应。

XL26 backfilling the main characteristics of compactors are used before and after the frame hinged connections, four-wheel independent hydraulic drive, with a strong driving force and better through sexual; closed up garbage into the frame to avoid damage to machinery; key parts the use of the internationalization of procurement, in order to improve the intrinsic quality of the machine, thus greatly reducing the frequency of repair machinery; before the frame is equipped with auxiliary leveling shovel to push the level to unload solid filler; cab comfortable, all-sealed design, with have air-conditioning and heating and ventilation features have deodorant with anti-rollover protection device with a better operating environment; In order to facilitate the repair and maintenance, in the engine hood and the vehicle chassis set up a number of door and window repair; on both sides with spacious solid barrier for operators and maintenance personnel to provide a convenient platform for security.

XL26回填压实机主要特点是采用前后车架铰接连接，四轮独立液压驱动，具有极强的驱动力和较好的通过性；封闭式车架避免垃圾搅入而损坏机器；关键零部件采用国际化采购，以提高机器的内在品质，从而大大减少机器的维修频次；前车架上装有辅助整平铲，用以推平卸下的固体填料；驾驶室宽敞舒适、全密封设计，配有冷暖空调且有除臭换气功能，带有防翻滚保护装置，具有较好的操作环境；为便于维修保养，在发动机罩和车架上设置了多处门和维修窗口；两侧配有牢靠宽敞的护栏，为操作人员和维修人员提供了方便安全的平台。

The X-ray diffract was used to analyze the crystal structure of the composite. Results show that after the talc was treated with blend coupling agent, the crystal structure of talc changed, and effect Intercalation phenomenon.

通过X射线衍射分析可知，用混合偶联剂对滑石粉进行预处理后，滑石粉晶体结构发生改变，产生了插层现象，使PP／滑石粉复合材料为纳米复合材料。

An authentic Maui wedding might require the services of a 'kahu', which is a Hawaiian minister.

毛伊可能需要一个真实婚礼服务'kahu'，这是夏威夷部长。