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With strain, elevating a candlestick: with pain, feeling on his right temple a contused tumescence: with attention, focussing his gaze on a large dull passive and a slender bright active: with solicitation, bending and downturning the upturned rugfringe: with amusement, remembering Dr Malachi Mulligan's scheme of colour containing the gradation of green: with pleasure, repeating the words and antecedent act and perceiving through various channels of internal sensibility the consequent and concomitant tepid pleasant diffusion of gradual discolouration.

420他心情紧张地举着烛台，感到疼痛伸手摸了摸肿胀起来的右颞叶撞伤处。他全神贯注地凝视着那庞大笨重被动的和那细溜活泼主动的，又殷勤地弯下身去，把掀起来的地毯边儿舒展成原样。他兴致勃勃地记起玛拉基。穆利根博士的色彩计划，其中包括深浅有致的绿色。他又心怀喜悦之情重复着当时相互间的话语和动作，并通过内部种种感官，领悟着逐渐褪色所导致的温吞快感的舒散。

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

Dear Daughter, since thou claim'st me for thy Sire, And my fair Son here showst me, the dear pledge Of dalliance had with thee in Heav'n, and joys Then sweet, now sad to mention, through dire change [ 820 ] Befalln us unforeseen, unthought of, know I come no enemie, but to set free From out this dark and dismal house of pain, Both him and thee, and all the heav'nly Host Of Spirits that in our just pretenses arm'd [ 825 ] Fell with us from on high: from them I go This uncouth errand sole, and one for all Myself expose, with lonely steps to tread Th' unfounded deep, and through the void immense To search with wandring quest a place foretold [ 830 ] Should be, and, by concurring signs, ere now Created vast and round, a place of bliss In the Purlieues of Heav'n, and therein plac't A race of upstart Creatures, to supply Perhaps our vacant room, though more remov'd, [ 835 ] Least Heav'n surcharg'd with potent multitude Might hap to move new broiles: Be this or aught Then this more secret now design'd, I haste To know, and this once known, shall soon return, And bring ye to the place where Thou and Death [ 840 ] Shall dwell at ease, and up and down unseen Wing silently the buxom Air, imbalm'd With odours; there ye shall be fed and fill'd Immeasurably, all things shall be your prey.

" 他说罢，母子俩大为高兴，"死"一听说有这样可以吃饱的地方，不觉微笑露出了狰狞的牙齿，预祝自己的肚皮将交好运，邪恶的母亲也同样欢喜，这样对她父亲说： V2：她说罢，聪明的魔王便警悟，立刻放温和些，这样委婉地答道，"亲爱的女儿，你既然叫我父亲，又把俊美的儿子指给我看，这是我在天上和你嬉游时的纪念品，当初的欢喜、甜蜜，今天已不堪回首，都只因这不可预料的不幸变故。要知道，我这次来不是要同你们敌对，而是要把你们和一切为正当的权利而武装起义，并从天上坠落的天使军的精灵们解放，从黑暗和痛苦中解放出来。

The excellent and good rate was 89.13%. In the DHS, there were 2 cases with greater trochanter fractures, 1 case with over boring, 2 cases with pulmonary infections, 2 cases with urinary system infection and 1 case with coxa vara. In hollow pressing whorl bolt internal fixation, there were 1 case with coxa vara and 1 case with deep phlebothrombosis.

采用动力髋螺钉固定者术中大转子骨折2例，髋螺钉孔钻孔过深1例，术后发生肺部感染2例，泌尿系感染2例，髋内翻1例；采用空心加压螺钉固定者术后出现髋内翻1例，发生深静脉血栓1例。

The excellent and good rate was 89.13%. In the DHS, there were 2 cases with greater trochanter fractures, 1 case with over boring, 2 cases with pulmonary infections, 2 cases with urinary system infection and 1 case with coxa vara. In hollow pressing whorl bolt internal fixation, there were 1 case with coxa vara and I case with deep phlebothrombosis.

采用动力髋螺钉固定者术中大转子骨折2例，髋螺钉孔钻孔过深1例，术后发生肺部感染2例，泌尿系感染2例，髋内翻1例；采用空心加压螺钉固定者术后出现髋内翻1例，发生深静脉血栓1例。

Thus Indra, the chief of the gods, was regarded as the regent of the east; Agni, the fire, was in the same way associated with the southeast; Yama with the south; Surya, the sun, with the southwest; Varuna, originally the representative of the all-embracing heaven, now the god of the ocean, with the west; Vayu, the wind, with the northwest; Kubera, the god of wealth, with the north; and Soma with the northeast.

从而因陀罗，众神之主，被认为是东方的摄政者。

Said simple building comprises building body, roof and pool inside room; the lower building body is complete closed, the upper building body is provided with pillar on lower building body, and is circled with sun-shading net around pillar, the outside of sun-shading net is equipped with movable curtain; the roof is straw roof and has two sides along ridge; the pool inside the room comprises cultivation pool and laying-up basin, each cultivation pool is provide with a overflow bench, the bench surface is equipped with a cushion and the cushion is fixed on slope bench with a plurality of bracing column of same height, and a bamboo curtain is placed on the bracing column for young wood frog rest.

该简易房，包括房体、屋顶和室内池，其下房体为全封闭体；上房体是在下房体上设有房柱，再用遮阳网沿房柱排列的走向围拢，在遮阳网的外面还设有活动帘；屋顶为稻草屋面，沿屋脊坡向两面；室内池包括养殖池和蓄水池，每一个养殖池中均设有漫坡台。坡台面上设有软垫，并有相同高度的多根支撑柱钉穿过软垫固定在坡台上，支撑柱钉上再放置供幼蛙栖身的竹帘。

Soil available N was positively correlated with spore density of Salvia miltiorrhiza and Dioscorea opposite, and hyphal and total percent colonization of Adenophora borealis, while negatively correlated with arbuscular colonization of Aster tataricus; Soil available P exhibited a positive correlation with arbuscular colonization of Salvia miltiorrhiza and vesicular colonization of Dioscorea opposite, while a negative correlation with spore density of Platycodon grandiflorus and vesicular colonization of Atractylodes macrocephala; Spore density of Bupleurum Chinense had a positive affection with soil organic matter; Soil pH was negatively correlated with hyphal and total colonization of Adenophora borealis and spore density of Bupleurum chinense, while positively correlated with spore density of Adenophora borealis.

土壤碱解N与丹参和麻山药的孢子密度、北沙参菌丝定殖率和总定殖率呈显著正相关，与紫苑丛枝定殖率呈显著负相关。土壤速效P与丹参丛枝定殖率、麻山药泡囊定殖率呈显著正相关，与桔梗孢子密度、白术泡囊定殖率呈显著负相关。土壤有机质与北柴胡孢子密度呈显著负相关。土坡pH与北沙参菌丝定殖率和总定殖率、北柴胡孢子密度呈显著负相关，与北沙参孢子密度呈显著正相关。

The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。