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Patients with clinical virologic breakthrough during NA therapy and 4 negative control patients without virologic breakthrough were selected for the study of quasispecies dynamics. All the 70 serum samples from these 10 patients were analyzed with the method of'PCR-cloning-sequencing'. Totally over 2,100 clones were sequenced. The target sequences obtained were aligned, compared and analyzed to study the mechanism of NA drug resistance and to understand the clinical course of NA therapy from a viral populational genetic point of view.

我们进一步采用国际认可的&金标准&&PCR-克隆-测序-序列分析&的方法，对6例发生临床病毒学突破的患者以及4例未发生临床病毒学突破的患者的全程血清样本进行了准种检测，共测序2100余个，以系统深入地对LAM和ETV治疗过程中HBV种群的演变及其临床意义进行探讨。

Chapter 5, from a cognitive point of view and integrating the analytical points covered in the previous chapters, examines the metaphoric nature of intentional humor which serves in a sub-discourse as ideational and/or interpersonal means of rhetoric under the global discourse of public speaking, with case analyses of such humorous metaphors, standard or non-standard, at the word, sentence, and discourse levels as well as at the meta-level, and with the idea of resemblance proximity to classify humorous metaphors into synecdochic, metonymic and metaphorical types.

第五章从认知语义的角度并结合前三章的分析来讨论公众演说者有意幽默的隐喻性，考察标准和非标准幽默隐喻在词汇、句子、话语层面以及在元层面的表现，并结合演说语料的个案分析考察幽默隐喻作为次话语在演说整体话语中所达到的概念修辞和人际修辞效果。基于隐喻的相似度，本章还将幽默隐喻进一步分类为借代性幽默、换喻性幽默，和隐喻性幽默。

The main achievement is tabling a proposal to the athletic load evaluation system from the point of view of compute evaluation, combining with my own understanding with the theory of sports load and sports training control.

主要研究成果在于，从计算机综合评价的角度，结合本人对运动负荷和运动训练控制理论概念的理解，对运动负荷的评价体系及评价指标提出了改进意见。

Joseph Stalin said makesconstant initiality of the view that any phenomenon if orphaned land, there is no association with the phenomenon of around it, it would not be able to understand it, because the nature of any given field of any phenomenon, if it is out of touch with the surrounding conditions, and they are isolated phenomenon society become meaningless things; on the contrary, any phenomenon if the same phenomenon has around, unviolable is subject to the constraints surrounding phenomenon, and that's understandable, arguably.

斯不小林说：&形而上学觉得，板滞界的任何一种不隐象，如果被簇拥不天、同边际不隐象操纵干系不天来看，那不离无法抱怨，因为板滞界的任何范畴洋的任何不隐象，如果把它看作是同边际前提操纵干系，与它们分隔的不隐象，那社会成为不十足事理的工具；反之，任何一种不隐象，如果把它看作是同边际不隐象有着不可不合割的干系，是施边际不隐象所制约的不隐象，那不离可以抱怨，可以论证了。&

From the ideology angle of view, combined with the characteristic of the medium dissemination, this paper expounds the "authenticity" of medium dissemination from the following three aspects:(1)"Individual viewpoint does not represent that of the station";(2)"Poets" steeple outdoes garret of parties;(3)Medium function the authenticity of medium dissemination with Chinese characteristic.

该文从意识形态的视角，结合媒介传播的特性，从三个方面对媒介传播的&真实性&进行了深入的讨论。第一，&个人观点不代表本台&；第二，&诗人的尖塔高出党派的阁楼&；第三，媒介功能－中国特色的媒介传播的真实。

The Guangxi Cities Constructions School network which in view of the technique of ASP, has been changed the former dynamic network forms,instead of presenting with the form of the adoption dynamic technique, each module linked mutually and became an organic whole,at last, with unify, tidy of forms present to all browsings.

基于 ASP技术的广西城市建设学校网站，改变以往的静态网站方式，以采用动态技术的形式呈现，各个模块间相互连接而形成一个有机整体，以统一、整齐的方式呈现给所有的浏览者。

The value of Blooming of Lantana lies in the characteristics of its thick woman creation and inspecting life with the view full of motherhood to taste life, furthermore in its peculiar narrative word and embodies woman to create with "white ink".

马樱花开》的价值也就在于其浓郁的女性创作特征，在于用充满母爱的眼光去观察生活，去聆听生命；还在于其独特的叙事话语；体现出女性用&白色墨汁&倾心创作的意味。

Pharaoh's Memorial with a lion-headed, it is a symbol of wisdom increases strength, Meanwhile an odd image is also in line with the spirit of the ancient Egyptians that is the view of the Pharaohs.

狮身加上法老的头像，则是智慧加力量的象征，同时这种奇特的形象也符合了古埃及人认为法老既是神又是人的观点。

In the term of transform of institution, cultural and positivism in urban studies of China today, the view of researches must pay attention on self-organization rules of urban space with socialites, in order to realize the unity of subjectivity and objectivity and the scientific researches of urban space with social logical.

提出在当前国内城市空间研究呈现制度文化转向和科学实证转向的双轨转型时期，通过对蕴含社会性的城市空间自组织规律的研究，有利于实现研究对象主客体属性的统一，使城市空间研究具有社会逻辑下的空间科学性。

View from the start with this care, using Noddings" care moral education theory, in light of the status of the school caring lack, it provides a three-pronged constructive ideas with the promotion of harmonious campus building: a "care" at the core of moral philosophy, for the harmonious campus building ideological driving force ; Constructing the teacher-student relationship of mutual care, nurturing building "harmonious campus";"exploring ways and means of moral humanistic care education to build a harmonious campus culture.

主要是从关心的视角入手，运用诺丁斯关心德育理论，结合我国学校校园关怀缺失的现状，对促进和谐校园建设提出了三方面建设性的构想：树立&关心&为核心的德育理念，为和谐校园建设提供思想动力；构建相互关心的师生关系，培育建设&和谐校园&的主体力量；探索体现人文关怀的德育途径和方法，建设和谐校园文化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。