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According to the result, we believed that the reason resulted in the low sensitivity was that the marker residue compound MQCA structure has changed after conjugated with protein and the antibody induced by the conjugation could not recognize the free MQCA in the ci-ELISA. Therefore we designed some hapten which could reserve the marker residue structural speciality mostly. After synthesis route selection and optimum, the hapten 7-Hydroxymethyl-3-methyl-quinoxaline-2-carboxylic acid was synthesized, used 4-methyl-2-nitroaniline as the start material via six steps, such as oxidation cyclization, bromize reaction, hydrolysis, Beirut reaction, reduction, hydrolysis. This work established basis for producing the high affinity antibody and developing the sensitive ELISA residue detection method.

本论文认为导致ELISA检测灵敏度较低的原因，是残留标示物和蛋白偶联后的特征结构发生改变，诱导产生的抗体不能够对游离MQCA很好的识别，因此，本论文设计了能够最大化保留MQCA结构特征的系列半抗原结构，并对其合成路线进行了筛选和优化，最终以对甲基邻硝基苯胺为起始原料，经氧化环化、溴代、水解、Beirut缩合、还原、水解六步反应制备含羟甲基结构的MQCA衍生物7-羟基甲基-3-甲基-喹噁啉-2-羧酸，为制备对游离残留标示物高亲和力的抗体，高灵敏度的ELISA残留检测方法的建立奠定基础。

Result: The incidence rate of OM was 72% in 25 patients, Bucca and tongue were areas that OM were more freguently developed on .The severe degree of OM was corresponding with the developed and lasting time.That was ,the earlier it developed and the longer it lasted, the more serious the OM was.

结果：25例病人中口腔粘膜炎发生率为72％，发生部位最多见于颊部和舌部，发生时间、持续时间和OM的严重程度有关，发生越早，持续时间越长，OM程度越重。

As for the realization of hardware,the thesis depicts the realization of every partof music recognition system based on the TMS320VC5402 in detail;as for thedevelopment of software,the thesis gives the software design flow chart of the musicrecognition system,simulates the basic theory with MATLAB language and gives thesimulation results.Well,with the characteristic parameters of the vocality and theinstrumental music,we can recognize the input digit music speech successfully andput forward it's pitch,cadent,and sing name.The result is comform that the musicrecognition based on the TMS320VC5402 is run well and the accuracy reaches 96percent.and it can meet the needs of the practicality application.

在硬件实现上，详细阐述了基于TMS320VC5402DSP的嵌入式音乐识别系统的各部分硬件设计，在软件开发上，给出了嵌入式音乐识别系统软件设计的各部分流程，并对各部分进行了仿真试验，给出了仿真结果，并分别对声乐和器乐这两种音乐信号进行了模板特征参数提取及其与待测信号进行匹配识别，试验结果表明：对音乐信号的识别精度在96%以上，成功实现了对音乐的音高、唱名、节奏的识别，满足了实际应用的需求。

The result shows that the best reaction conditions for synthesizing CMC with a high degree of substitution (1.68) are that the ratio of chitosan to sodium chloroacetate is 1:5, reaction temperature is 90℃, reaction time is 5h, and the amount of catalyst is 3%.

研究了投料比、反应温度、反应时间、催化剂等单因素对取代度的影响，合成高取代度的最佳反应条件为m：m=1：5、温度90℃、时间5h、催化剂量为3%，可制得取代度为1.68的羧甲基壳聚糖。

The optimum condition was determined via orthogonal experiments, and the result shows that the technology of bile extraction and purification adopts 10L chromato bar with 3L bile, gel silica as fixed phase, the mixed chloroform and isopropanol as mobile phase, gradient elution with mobile phase (the mobile phase ratio in extraction of cholalic acid and glycocholic acid is 4:1 , and then becomes 3:2 in extraction of glycocholic acid and glycocholic acid), and eluting velocity of 3～5mL/s .

采用正交试验筛选了柱层析最佳条件，以容积为10L的层析柱进行分离纯化，固定相为硅胶，流动相为氯仿和正丁醇混合溶剂，一次装样量3000mL胆汁，流动相采用梯度洗脱，先按4：1比例分离胆酸和甘氨胆酸，然后按3：2比例分离甘氨胆酸和牛磺胆酸，流动相洗脱速度为3～5mL/s。

The total RNA from brain tissue of substantia nigra of PD rats is extracted at the 1st, 2nd, 4th and 8th week and comparison is made between it and that from normal rats. The total RNA, after RNA formaldehyde degenerated sepharose electrophoresis and separation and Northern transfer, is adsorbed unto colloxylin membrane. Then ECL labeled Selenoprotein P and an unknown gene fragment are used as probes to hybridize with the RNA on the colloxylin membrane. And the hybridized result is obtained with enzyme-linked immunosorbent assay. Finally, analysis of OD is made using ONE-Dscan software.

分别提取1周、2周、1月、2月的帕金森大鼠（各8只）黑质区脑组织总RNA，并取相对应时间的正常大鼠的脑组织总RNA作为对照，经RNA甲醛变性琼脂糖凝胶电泳分离后，通过Northern转移，将其吸附在硝酸纤维素膜上，然后利用ECL标记的Selenoprotein P和一段未知基因为探针，与硝酸纤维素膜上的RNA分子进行杂交，再通过显影取得杂交的结果，应用ONE-Dscan软件进行光密度值分析。

The relative cell groth rate of L929 cell represented the proliferation of cells on materials with respect to control over time. The result showed that cytotoxicity of thermo-sensitive cell culcure dish was none or little,which can be used for lab use.Additional cell culture was performed with two types of celland cell sheets detachment were observed by reducing the culture tempareture after the confluently growth of the two types of cells.

进一步利用两种不同的细胞永生化的成牙本质细胞系和人牙周膜细胞在接枝有PNIAPm的培养皿上进行培养，观察到细胞可以正常贴壁，生长并增殖，并且在形成连续的细胞层之后，通过降低温度，获得了完整的细胞层结构，结合前两部分实验结果，证实了利用等离子体预处理和紫外线照射接枝制备温敏性培养皿的可行性，为后续研究提供了实验基础。

In othor way, we use RT-PCR to observe the secretion of mRNA to compare with the balance of TH1/TH2 in spleen, so that we can see if we treat with cystamine can apparently raise the ratio of TH1 and also raise mRNA of CD25/ Foxp3, but reduce mRNA of CD40L. As a result of kidney which is the important secondary immunity organ discusses cystamin whether is the important control action or not to regulate in kidney then to be able to improve illness.

另外，透过了反转录聚合链锁反应(reverse transcription-polymerase chain reaction， RT-PCR)观察spleen中CD8/CD4 maker的mRNA表现量比较TH1/TH2 balance的倾向，发现经过胱胺处理后的确TH1相关的CD8 maker的mRNA比例量有大幅度上升，间接证明SLE倾向於TH2有被反转，而在Treg的特异性 maker方面CD25/ Foxp3之mRNA之比例也有大量上升，T cell的活性化maker CD40L也减少了mRNA的表现量，同时证实了SLE免疫倾向的反转，及发炎情况的减少及Treg cell的大量诱发。

On the basis of census of all individuals of the populations at different plots, the structure and dynamics of the populations were described. The result showed that P. tabulaeformisf.shekannesis were growing populations, and the distributions of the structure were reverse J. The density decreased with the increasing of Size-class structure. The populations developed the characteristic of increasing in the early stage and stabilizing in the late. The survive curve of Pinus tabulaeformisf.shekannesis were between DeeveyⅡand DeeveyⅢ. The analysis of time sequence showed that most of the inividuals would be adult after 20 years.

结果表明：柴松种群结构呈反J分布型，种群处于增长阶段；种群的密度随径级的增加而降低，种群密度与径级呈现出明显的负相关；四个函数估计值说明柴松种群具有前期增长、后期稳定的特点，其存活曲线介于DeeveyⅡ型和DeeveyⅢ型之间；随时间推移，种群中树和大树数量逐渐增多，种群在20年后开始大量进入成熟阶段。

However, as a result of human activity especially moorland is reclaimed, the aggravate that uses favourable geographical position waiting for earth of chemical fertilizer to use an activity in great quantities, and large-scale disafforest and habitat are broken the change that spends favourable geographical position waiting for earth to use structure, played havoc with natural landscape, the change moves floral to live environment, make ecosystem function degrades, brought about the forfeiture of biology diversity finally.

然而，由于人类活动尤其是荒地开垦、化肥的大量使用等土地利用活动的加剧，以及大规模砍伐森林和栖息地破碎化等土地利用格局的改变，严重破坏了自然景观，改变动植物的生存环境，使生态系统功能退化，并最终导致了生物多样性的丧失。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。