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So is it not with me as with that Muse Stirr'd by a painted beauty to his verse, Who heaven itself for ornament doth use And every fair with his fair doth rehearse Making a couplement of proud compare, With sun and moon, with earth and sea's rich gems, With April's first-born flowers, and all things rare That heaven's air in this huge rondure hems.

二一 我的诗神①并不像那一位诗神只知运用脂粉涂抹他的诗句，连苍穹也要搬下来作妆饰品，罗列每个佳丽去赞他的佳丽，用种种浮夸的比喻作成对偶，把他比太阳、月亮、海陆的瑰宝，四月的鲜花，和这浩荡的宇宙蕴藏在它的怀里的一切奇妙。

So is it not with me as with that MuseStirr'd by a painted beauty to his verse,Who heaven itself for ornament doth useAnd every fair with his fair doth rehearseMaking a couplement of proud compare,With sun and moon, with earth and sea's rich gems,With April's first-born flowers, and all things rareThat heaven's air in this huge rondure hems.O' let me, true in love, but truly write,And then believe me, my love is as fairAs any mother's child, though not so brightAs those gold candles fix'd in heaven's air: Let them say more than like of hearsay well; I will not praise that purpose not to sell.

我的诗神①并不像那一位诗神只知运用脂粉涂抹他的诗句，连苍穹也要搬下来作妆饰品，罗列每个佳丽去赞他的佳丽，用种种浮夸的比喻作成对偶，把他比太阳、月亮、海陆的瑰宝，四月的鲜花，和这浩荡的宇宙蕴藏在它的怀里的一切奇妙。哦，让我既真心爱，就真心歌唱，而且，相信我，我的爱可以媲美任何母亲的儿子，虽然论明亮比不上挂在天空的金色烛台。谁喜欢空话，让他尽说个不穷；我志不在出售，自用不着祷颂。

Penances ought to be joined with the idea of labour, not with that of idleness; with the idea of good, not with that of supereminence; with the idea of frugality, not with that of avarice.

忏悔应当与劳动的观念相结合，而不应该与闲散的观念结合；应当与良善的观念结合，而不应当与出类拔萃的观念结合；应当与节俭的观念结合，而不应当与贪婪的观念结合。良好的人性，恰当的理性，和对上帝的信仰。

Sequence analysis and phylogenetical study showed that this cDNA contained 778 nucleotides, with a 627 bp open reading frame flanked with a 44 bp 5' UTR and a 107 bp 3' UTR. The encoded 208 amino acids housefly Attacin shared a high similarity of 50%~70% with that of the other dipterous insects. In addition, the phylogenetical analysis also indicated that the Attacin from housefly was in the same branch with those of other species, suggesting that they come from the same ancestor.

家蝇Attacin的cDNA全长778 bp，其中包括627 bp的开放式读码框，44 bp的5ˊ末端非编码区(5ˊUTR)和107 bp的3ˊ末端非编码区(3ˊUTR)，相应的蛋白产物含208个氨基酸；与其他双翅目昆虫的Attacin具有很高的相似性，约50%～70%；以邻接法(Neighbor-Joining，NJ)所构建的系统关系表明家蝇的Attacin与其他双翅目昆虫的Attacin起源于共同的祖先。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Through designing of composition and structure of the bioactive graded coating,innerstress and its distribution in the coating were analyzed and calculated, the resultsshowed that when composition distribution coefficient n was 1.5, a reasonable stressdistribution could be got, that was at the beginning of deposition the suspension containingrichly BG granules was used so that a rich BG granules layer, a good transitional layerbetween BG layer at the bottom and the coating could be obtained at the titanium alloy side,the bottom of the coating; the stress value near the interface and surface and its character,pressure stress or tensile stress, were decided by the character of its composition itself.Changing composition distribution coefficient n could only change the variation tendency ofstress in the coating, but did not change the stress distribution rule in the coating. Thethinner the coating is, the sharper stress variation in the coating is, which does not mean thatthicker coating is better because the thicker the coating is, the little the permitteddeformation of coating is, so the coating thickness should be thinner, for example, about50μm for bending applications, but for applications only bearing pure shear stress, such asroot of tooth implant, the coating can be thicker little, for instance, about 80～100μm. The study on electrification characteristic and electrophoresis deposition of HAand BG granules in aqueous and non-aqueous solution system found that EPD almost didn'toccur in aqueous solution system. However, because HA granules take position charges inabsolute alcohol, a homogeneous EPD be carried out on the cathode titanium alloy slice, but taking negative charge in absolute alcohol the BG granules not be deposited on the cathode. A guided HA crystallizing, 100～300nm, on surface of the BG granules be realized by metathetical reaction, which cover BG granules with HA microcrystals and make the covered BG granules taking position charges in absolute alcohol, sequentially realize the EPCD of the BG and HA granules on the cathode, so it is feasible to make a titanium alloy/BG/HA bioactive graded coating by making use of EPCD technology. The corrosion experiment of rich boron bioglass coating and plasma spray coating showed that split phase, rich boron and rich silicon phase, occurred during its preparation. In basic medium the corrosion behavior of 〓 BG coating showed uniformity corrosion, the corrosion mostly occurred at rich boron phase area, therefore batch formula design of BGshould avoid the occurring of split phase. The corrosion appearance of plasma spray coatingappeared a non-uniform corrosion, mostly occurred at the edge of the laminated HA moltendrops, and emerged an accelerated corrosion tendency, which will easyly lead to corrosioncrackles extending to the interface and the happening of osmotic interfacial corrosion, thatmay be one of the major reasons leading to the coating cracking-off in the later period. Thetesting results of thermal expansion coefficient of 〓 and 〓BG showed the thermalexpansion coefficient of 〓 BG matched with that of titanium alloy better, and 〓 BG couldsinter with titanium alloy into densification enamel layer at low temperature (720℃).

将Ti6Al4V合金在1000℃下进行真空热处理会降低其力学性能，且合金内的V元素会向表面富集，因此，钛合金真空热处理和表面涂层的烧结温度不能过高，即应低于其相转变点；通过对生物活性梯度涂层的组成和结构的设计，分析和计算了梯度涂层内的应力大小和分布，结果表明：对于本研究，当成分分布系数n=1.5时，可以获得较合理的涂层力学性能，即在沉积开始时，采用富含BG颗粒的悬浮液，以便在钛合金侧获得同底层BG有良好过渡的富BG涂层；梯度涂层界面和表面的应力大小、性质由材料组成本身的性质决定，改变成分分布系数，只能改变涂层内应力变化的趋势；涂层的厚薄不影响涂层内的应力分布规律，但涂层越薄，涂层内的应力变化越快，但这并不意味着涂层越厚越好，因为涂层越厚，涂层允许的变形越小，对于应用于弯曲受力部位的涂层而言，涂层应薄一点为好(50μm)；而对于仅纯受剪切应力的部位，如牙根种植体，涂层可适当加厚(80～100μm)；通过对HA和BG颗粒在水溶液体系和非水溶液体系中的带电特性和电泳沉积的研究发现，它们在水溶液体系中很难发生电沉积；在无水乙醇溶液中，HA颗粒带正电，可在阴极钛合金片上发生均匀的电泳沉积，而BG颗粒则带负电荷；利用复分解反应法，可以制得100～300nm的HA，通过诱导HA在BG颗粒表面结晶，可对BG颗粒进行表面包覆，获得了被HA包覆的BG颗粒，改变了BG颗粒表面的带电特性，使BG和HA颗粒在无水乙醇中均带上正电荷，从而实现了HA和BG颗粒在阴极上的共沉积。

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果： hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙甙，对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙甙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙甙组相比，统计学上无显著差异(P＞0.05)。hTERT反义核酸作用于CEM细胞24 h加入顺铂，再共同作用48 h，CEM活细胞均数为2.318×108 cells/L，与单用顺铂组(3.250×108 cells/L)及hTERT正义核酸联用顺铂组(3.175×108 cells/L)相比，对细胞抑制明显增强(P＜0.05)。hTERT ASODN作用于CEM细胞24 h再加入顺铂作用48 h，细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L顺铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与顺铂联合作用组(6.97%)、单用顺铂作用组(6.02%)进行比较有显著差异(P＜0.01)。

Penances ought to be joined with the idea of labour, not with that of idleness; with the idea of good, not with that of supereminence; with the idea of frugality, not with that of avarice.

忏悔应当与劳动的观念相结合，而不应该与闲散的观念结合；应当与良善的观念结合，而不应当与出类拔萃的观念结合；应当与节俭的观念结合，而不应当与贪婪的观念结合。

Comparing the landscape types of 1995 with that of 2005, we find out that:①the natural arbors are decreasing, with patches more fragmentation, size more odds, shapes more complex;②the area of natural shrubberies is increasing, with patches less fragmentation, shapes more simple and formulary ,the size of patches more even;③the area of natural grasses is increasing, with patches less fragmentation, shapes of patches more odds;④the area of timbers is increasing, with patches more dominance in landscape, more connectivity;⑤the area of Eucalyptus increases, with patches less fragmentation, shapes of patches more complex , more convergence, more connectivity;⑥the area of orchards is increasing, more connectivity;⑦the patches of the rubber is becoming more assemble, more connectivity, more formulary;⑧the area of plowland increases;⑨the area of reservoirs, rivers and ponds increases, with patches more assemble;⑩the area of cities decreases, with patches more dispersing, with shape more complexthe area of countries increases, with patches less connectivity, more dispersing, with shape more complex; the patches of the rest lands are more dispersing, with shape more complex;(3)In the process of economic development in the upper reaches of the Changhuajiang river watershed, a series of the ecological environment problems have appeared because a lot of landscape function broke off, and the connection of human landscape with natural landscape decreased, which aroused by the fragmentation of natural and semi-nature landscapes increased, the stability of the landscape structure decreased, as well as stress of human disturbance, resource and environment upon the landscape increased gradually.

3昌化江上游流域在经济发展进程中，自然与半自然景观破碎度增加，自然景观结构稳定性下降，人为干扰、资源和环境对景观胁迫逐渐增大，中断了许多生态过程，导致城市等人文景观与自然景观连接度很差，产生了一系列的生态环境问题。

On the termination date, the cultured explants were all examined by Western blot, HE and transmission electron microscope. Our results showed that after 12-days in culture, the cultivation treated with AS-ODN reduced the synthesis of AMBN and had a deformed dental cusp with thinner enamel matrix. Ultrastructure analyses showed that there was hardly any cisternae of the rough endoplasmic reticulum in the ameloblasts at the tip of the cusp of AS-ODN treatedexplants. However, on average the enamel matrix was thinner compared with that in the control group. Furthermore, the collagen fibers in extracellular matrix were found disorganized. These findings seemed to provide a direct experimental evidence that tended to indicate that the arrested AMBN translation in cultured tooth germs might result in the delay of the tooth development.

经用Western蛋白印迹检测表明，所设计的反义核酸对AMBN InRNA具有良好的封闭效果并成功阻断了牙胚对AMBN的表达；在缺乏AMBN情况下，与对照组相比，实验组牙胚在体外可以继续生长发育至钟状晚期，出现成釉细胞和成牙本质细胞的分化，成釉细胞可以分化成为分泌期型成釉细胞，胞浆中缺少合成蛋白质所必需的粗面内质网和高尔基氏体，缺乏溶酶体，表明对蛋白合成和脚的能力降低；实验组牙胚有牙尖形成和基质分泌，但牙尖形态异常，基质形成减少，牙尖周围基质最厚处为O.6卜m，明显薄于对照组的5.spin，基质中胶原纤维粗细不等，排列稀疏， 3 第四军医大学硕士学位论文未见钙化现象，充分证明了AMBN在牙胚发育中参与釉质基质形成和矿化过程，影响胶原纤维和牙本质基质的合成，促进成釉细胞对蛋白质的合成和釉质基质蛋白降解。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。