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Use constituent piece to stick mural way depart to foster Niu Er to become fiber cell above all, after classics generation is rarefied, fat simple way will be contained double the skin of the 4th acting ox that outside conveying carrier to turn into body, the particularity of person bacteriolysis enzymatic mammary gland that chooses number fosters becomes fiber cell, g/mLG418 chooses 500 μ 2 weeks, g418 halve continues to develop 3 generation of 2 ～, catch the cell after choosing to undertake PCR detects to turning next reach nucleus analysis.

首先采用组织块贴壁法分离培养牛耳成纤维细胞，经传代纯化后，脂质法将含有双重筛选标记的人溶菌酶乳腺特异性表达载体转入体外培养的第4代牛皮肤成纤维细胞，500μg/mLG418筛选2周，G418减半继续培养2～3代，然后对转染筛选后的细胞进行PCR检测及核型分析。

Reach what the SPF class small rat that its feed undertakes bacterium and helminth 1 year to monitor to this system continuously, live to what bandicoot undertakes imitate cuts off the power and bacterioscopy, be in to raising the comparative test that the small rat of SPF class KM of traditional protective screen system and IVC system has immune index.

连续1年对该系统及其饲养的SPF级小鼠进行细菌和寄生虫的监测，对大鼠进行模拟断电的生存和细菌检查，并对饲养在传统的屏障系统和IVC系统的SPF级KM小鼠进行免疫指标的对比实验。

The daughter getting farther away in the Greyhound Is beyond where I can imagine. Interior running lights of phosphor-green Show a faint shape of her head, reflected on the glass, Against the Dakota night. I try to resolve the picture, but the Badland darkness Empties her silhouette. If she had to leave, it should have been in a perfect vehicle, But she rides that dirigible of the road, Dispatched by a breakdown scab corporation Into a triangle my wavelength will not reach. The sealed-off revolutions of engines Are faster propelling souls at night-time Than my sorting of memories Can fix a negative under ruby light. Yet right here I have a template that strained for her form Among a crowd of children at the schoolyard gate.

长途车上的女儿越来越远地驶出了我想象的范围仪表盘上绿色的微光和玻璃另一面达科达州的夜色拖显出她模糊的头型我想拉近她的形象但荒原的黑暗吞噬了轮廓里面的所有细节既然要走就该有更好的载体来迎接她的献出但破落的黄牛公司所派出那辆路上的飞艇把她载到我频道无法达到的三角区域这马达无情转动的悲凉在夜间输送灵魂时用更快的速度超越我对记忆的显影我用由她配制出来的烙印呼唤就像曾经在那群涌出校园门口的孩子中间我怎能不去努力搜索她所踏入的陌生区域的电波？

The sixth place at Bahrain was a decent result, but now we have to reach much higher.

在巴林第六名是一个体面的结果，但现在我们达到更高。

We plan to reach a new game for which we would need a metallic play baize .

为此，我们需要一个坚固的戏剧画面。

But fiscal conservatives within the CDU and its Bavarian sister party , the Christian Social Union, fret more about bringing back into balance a budget deficit that may reach as much as 6% of GDP this year.

而基督教民主联盟以及其巴伐利亚姊妹党中的财政保守分子，却认为解决财政赤字才是当务之急。今年财政赤字很可能达到GDP的6%。

What south and north experienced social custom below the condition in opposite independence is different evolve, in towel of hairdo, coronal, take make, the shoe walks on those who reach baldric cap-a-pie there is bright color on each component, thereby the relatively apparent otherness that shows different district to go up in dress custom.

魏晋南北朝时期，政治上的割据造成南北地域上的分裂。南方和北方在相对独立的状态下经历了社会风俗的不同演变，在发式、冠巾、服制、鞋履及佩饰从头到脚的各个组成部分上都有着自己鲜明的风格，从而较为明显的表现出不同地域在服饰风俗上的差异性。

Republicans and many conservative Democrats are balking on the cost of the reform, which could reach one trillion dollars over the next several years.

许多保守共和党人和民主党人都止步于改革的成本，可以达到在未来数年内1万亿美元。

Kutcher triumphed over CNN in their much ballyhooed race to be the first to reach a million followers on the microblogging Web site.

在库奇与CNN这场经过大肆宣传的比赛中，库奇最后获胜，成为第一个在微网志网站Twitter拥有百万名追随者的用户。

You reach in, there's one maneuver and bam!

你太妄下断语了，用点小技巧。

More direct, directer ; most direct, directest

径直的，笔直的

Do you know how to use a chain saw?

你知道如何使用链锯吗？