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All index proved that the P5776 cDNA library constructed in this research had high quanlity and could be used for further ESTs sequencing and analyzing. The amplified library of P5776 was screened on white-blue plates, and more than 5,000 white-clones were selected to culture in 96-well plates, then the high quanlity plasmids of white-clones were extracted by traditioal alkaline-lysis method using the Vitagene?96-easy plasmid extract kit. Through PCR primered by T3( the vector has T3 RNA polymerase promoter ), the target fragments of inserts were get. Then the inserts were taged by four different fluorescence-dye, and fractionated by capillary in ABI Prism? sequencer. More than 5,000 5′-ESTs had been sequenced and through rough selecting, 4,747 ESTs were remained for further research. Among the remained ESTs, more than 90% have longer lenth than 500bp, and there are less than 5% unable-read bases in every sequence.

将扩增好的P5776 cDNA文库铺布蓝白斑筛选平板,挑取其中的白斑经活化培养后,运用Vitagene 96-easy质粒DNA制备试剂盒以碱裂解法共提取出58 板(96孔板),即5,000 余个重组子克隆的高质量质粒DNA;提取好的质粒以T3(连接载体上具有该引物RNA聚合酶启动子)为引物经PCR(Polymerase Chain Reaction,聚合酶链式反应)扩增出重组子插入片段,标记上荧光染料后在ABI Prism 3100 测序仪上经毛细管电泳完成了5,000 余条5′端EST 序列的测定工作,初步筛选出4,747 条质量较好的EST序列,经筛选得到的EST中90%以上具有大于500bp 的可读序列,每一EST序列中不可读碱基数小于5%。

White-bearded captain who wants to help white beard, white beard, but launched a tsunami, the boat sent to exports.

白胡子的队长们想帮白胡子,但白胡子发动了海啸把船送到了出口。

Dyeing process is to use a knife dipping wax melted wax on the white cotton cloth depicting a predetermined pattern, the wax solution formed after solidification anti-pollution film, put cotton into the dye vat of dip several times , so that there is no wax to protect the white film was dyeing, printing and dyeing cloth after the boiling water, so that membrane fusion and drift away from the wax, cotton cloth, cotton cloth exposed color get a white pattern.

印染工艺是用蜡刀浸沾熔化的蜡液在白棉布上描绘预定的图样,蜡液凝固后形成防染薄膜,把棉放入染缸中浸染数次,使没有蜡膜保护的白布被染色,印染后的棉布用水煮沸,使蜡膜熔化而漂离棉布,显露棉布原色,得到白色图样。

Moreover, turnip, cabbage, white sprout of bean, the sprout of bean, the white spring onion, looks although belong to white, at Korean 5 colorses reduce weight in or Be returned to a green food.

另外,萝卜,白菜,白豆苗,豆苗,白葱,看起来虽属白色,但在韩式5色减肥中还是归于绿色食品。

Although the Song Dynasty tea bowl five glaze:黑釉, butter glaze, blue, white, white, but because of ease of set off lights黑釉white tea brown foam has been fighting to observe the love of tea.

宋代的茶盎虽有五种釉色:黑釉、酱釉、青、青白、白釉,但黑釉盏因便于衬托白色茶沫观察茶色而受到斗茶者的钟爱。

The white moon on the wane and the white star on the upper flag symbol the royal family, while the white sun on the lower flag originates from the symbol of the Rana Family.

上面的三角形旗中是白色弯月、星图案,代表皇室;下面三角形旗中的白色太阳图案来自拉纳家族的标志。

Keywords: Snake, Beauty and the Beast, Pop Star, Snake Girl, christianism, Sin, PC Game, Star Portrait, legend-of-white-snake, white-snake, snake-white, beautiful-girls, Adam and Eve

西方基督教有亚当与夏娃同蛇类的情感纠葛,中国亦有《白蛇传》的美丽传说(台湾还为此拍摄了风靡一时的电视连续剧《新白娘子传奇》)。

Keywords: Snake, Beauty and the Beast, Pop Star, Snake Girl, christianism, Sin, PC Game, Star Portrait, legend-of-white-snake, white-snake, snake-white, beautiful-girls, Adam and Eve

西方基督教有亚当与夏娃同蛇类的情感纠葛,中国白蛇传的美丽传说,台湾电视连续剧新白娘子传奇。

White-box: can be divided into a layer corrugated (3 or 5 layer) white-box and white box, free corrugated packaging after sealing General to tape.

白盒:可分为有瓦楞(3层或5层)白盒和无瓦楞白盒,产品包装后一般要用胶带封口。

Constant sea is Sinopec Maoming Petrochemical Co., Ltd.,"the South China Sea card" downstream products distribution unit, responsible for the delivery to the factory, price concessions, now the main products are:"the South China Sea card,"# 58,# 56 Food packaging wax,# 58,# 56 all refined white wax,# 58,# 56 white semi-refined paraffin, wax saponification, chlorinated paraffin, solvent oil, white oil, toluene.

茂名市恒海石油化工有限公司是中国石化茂名石油化工股份有限公司《南海牌》产品下游经销单位,负责送货到厂,价格优惠,现在主要产品有:《南海牌》58#、56#食品包装蜡,58#、56#全精炼白石蜡,58#、56#半精炼白石蜡,皂化蜡、氯化石蜡、溶剂油、白油、甲苯。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。