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The costs of inbreeding When closely related individuals interbreed,'inbreeding' occurs ,which gives rise to inbreeding depression (which causes a reduction in offspring fitness) and increased genetic homozygosity (which reduces the possibility of evolutionary adaptation to a changing environment, and increases the possibility of extinction).

近交的代价当亲缘相近的个体进行亲繁殖时，"交近"就出现了。近交引起近交衰退和增加遗传纯合性(它降低了对于环境变化产生进化适应的可能性，而增加了灭绝的可能性)。

When closely related individuals interbreed, 'inbreeding' occurs ,which gives rise to inbreeding depression (which causes a reduction in offspring fitness) and increased genetic homozygosity which reduces the possibility of evolutionary adaptation to a changing environment, and increases

近交的代价当亲缘相近的个体进行亲繁殖时，"交近"就出现了。近交引起近交衰退和增加遗传纯合性（它降低了对于环境变化产生进化适应的可能性，而增加了灭绝的可能性）。许多物种具有防止或减少近交的机制。

When closely related individuals interbreed,'inbreeding' occurs ,which gives rise to inbreeding depression (which causes a reduction in offspring fitness) and increased genetic homozygosity (which reduces the possibility of evolutionary adaptation to a changing environment, and increases the possibility of extinction).

当亲缘相近的个体进行亲繁殖时，"交近"就出现了。近交引起近交衰退和增加遗传纯合性(它降低了对于环境变化产生进化适应的可能性，而增加了灭绝的可能性)。

But after a day in which we saw the Quidditch camp, the Pensieve scene room, the underwater tank, the DADA classroom, Dumbledore's office, the Gryffindor dorm and common room (dressed this time, as opposed to last), models for to-be-built sets, a trophy room, the acres of costumes for the film, the 30-foot animatronic Horntail dragon, and more - after a day in which we met the blushing Robert Pattinson, composed Clemence Poesy and deceptively mild Stanislav Ianevski - after a day in which it became clear that Mike Newell is a mastermind at turning a flat scene into a hilarious one - we left Leavesden positively bouncing in anticipation.

但在一天后，我们参观了魁地奇营地，冥想盆场景的房间，水下装置，黑魔法防御教室邓不利多的办公室，格兰芬多的宿舍和公共休息室（与上次不同，这次都装饰一新），等待装配的模型，奖杯陈列室，戏服更换场地，30-英尺高的匈牙利树峰，那一天我们还看到了精力旺盛的Robert Pattinson，沉着冷静的Clemence Poesy，让人猜不透的温和的Stanislav Ianevski——那一天我们还了解到麦克纳威尔正策划把一个单调乏味的场景变得热闹非凡。

Howbeit that was not first which is spiritual, but that which is natural; and afterward that which is spiritual.

15：46 但属灵的不在先，属血气的在先。以后才有属灵的。

In general, the design work with traditional measures is done by hand, which cannot meet the needs of the technology and manufacture. So the author has developed a CAD hydraulic design software fit for lower specific speed axial flow runner, which is easily operated by users.The main research work of this paper is as follows:1 Analyzing the main reason of axial flow power station cannot work well under littler volume of flow in some season and putting forward an improving scheme, pointing out the characters of design under abnormal factors.2 Combined with the development of the industry in our country, the abnormal hydraulic design method of propeller turbine is discussed based on present literature, data and experiment results.3 Guided by the design method of software engineering, a suit of hydraulic design software of Lower Specific Speed Axial Flow Runner is programmed by the object-oriented programming language Visual Basic and a straightforward, concise and standard Windows human-computer dialogue interface is designed for the system, which makes the operation of the system very convenient.4 ActiveX Automation technology is adopted as the interface with AutoCAD and the hydraulic drawings are edited, modified by AutoCAD until hydraulic model drawings according with the demand of engineering practice are output.5 To resolve practice problem, the author has designed a lower specific speed axial flow runner for Duowa Power Station through an example.

论文的主要内容有：1 深入分析很多轴流定桨式水电站枯水季节小流量下不能发电的主要原因，提出减容改造方案，并指出超常规条件下的改造设计特点。2 结合国情及行业发展需要，参考现有资料、文献和前人的研究成果，进行低比转速轴流式转轮叶片水力设计方法的研究，对轴流式转轮叶片的奇点分布法水力设计进行了全面的探索，结合现代计算机技术，找到了一种针对特殊条件的轴流式转轮叶片水力设计方法。3 仔细比较多种AutoCAD二次开发工具后，结合本课题的特点，选用Visual Basic语言，综合应用软件工程的设计思想，进行低比转速轴流式转轮叶片水力设计软件的编制，为系统设计了直观、简洁和标准的Windows人机对话界面，便于用户操作；编程时在考虑到设计条件特殊性的同时，尽量做到程序的通用性，使之可用于解决同一类问题；另外，大部分程序还可移植到普通轴流式转轮叶片水力设计中，具有良好的适应性。4 利用ActiveX Automation技术与AutoCAD接口，对AutoCAD进行二次开发，在AutoCAD下实现水力图的自动生成和编辑，直至输出符合实际工程要求的木模图。5 通过算例，为多哇水电站设计了一个低比转速轴流定桨式转轮，解决其在枯水期小流量下不能发电的问题。

In YongKang,there is "No.1 Mount in ZheJiang",FangYan Mount,famous for magnificence and sheerness and DanXia landform;there is WuFeng School where ZhuXi and ChenLiang gave education and speech;there is ShiGuLiao TV City,which was built by government and people and have received film crews from"Dragon Story","Great HanWu","SongCi" and 100 other films or TV series;there is ShiCheng Mountain,the culture center of Emperor Huang in Southern China;there is folk-custom culture village,where you can enjoy "nine lions picture",taste"farmhouse foods" and play"dog husking rice";there is WuZhi Rock,which is described by "if looking from far distance,five mountains extend into sky,like a person reaches cloud by hand ";there is LingShan Lake Immortal Rock,which is said "mountains is like picture screen, waterfall sound is equal to thunder";there is XiJin Bridge,the longest stone pier,wood bridge,multi-eaves gallery bridge;there is gradually hot Special industry tour of "go famous garden to look renowned enterprise,visit famous city to buy hardware".

永康有以雄奇峻险、丹霞地貌著称的"浙东第一山"方岩山；有朱熹、陈亮曾讲学论理的五峰书院；有与民资共同开发已成功接待《天龙八部》、《汉武大帝》、《大宋提刑官》等100多部影视剧拍摄的石鼓寮影视城；有南中国黄帝文化中心石城山；有看"九狮图"、尝"农家饭"、玩"家狗舂米"的九狮沟民俗文化村；有"远望五峰插天，若人伸手探云"的五指岩；有"峰峦如画屏，瀑声赛雷鸣"的灵山湖仙岩，有全国最长的石墩木梁重檐廊桥西津桥；有日趋火曝的"到名园看名企、逛名城购五金"的特色工业游。

A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising subjecting said sample to an amplification reaction using a set of nucleotides, at least one of which is fluorescently labelled, contacting amplification product with a probe under conditions in which the probe will hybridise to said target sequence, said probe comprising a reactive molecule which is able to absorb fluorescence from or donate fluorescent energy to said fluorescent labelled nucleotide and monitoring fluorescence of said sample.

一种用于检测在某样品中是否存在目标核酸序列的方法，该方法包括：使用一套核苷酸对所说的样品进行扩增反应，其中至少一个核苷酸是被荧光标记的，在探针与目标序列杂交的条件下使扩增产物与探针混合，所说的探针包括一种活性分子，该活性分子能够从荧光标记核苷酸吸收荧光或向其贡献荧光能量，及检测样品的荧光。

The results show that:(1) the overall water quality in the Xiangjiang River had become worse during the 1990s and the main pollutants which caused aggravating pollution were total coliforms, total phosphorus, petroleum and total hydrargyrum ;(2) the main pollution sources were living pollution, which increased evidently in recent years, and industry pollution, of which heavy metal pollution is evident;(3) among the mainstream of the Xiangjiang River , river sections in Zhuzhou, Xiangtan and Changsha suffered from the most serious pollution;(4) it is forecasted that living pollution and agriculture pollution will become more serious in the future.

结果表明：湘江总体水质在20世纪90年代呈恶化趋势，致使污染加剧的主要因子是总大肠菌群、总磷、石油类和总汞；主要污染源为工业污染和生活废水污染，近年来生活废水污染日益突出，工业污染中重金属污染明显；在干流不同河段中，株洲、湘潭和长沙河段污染最为严重；估计未来湘江的生活污染和农业污染还会加剧。

Some paper, the Pall Mall Gazette I think, describing the dress-rehearsal of one of my plays, spoke of you as following me about like my shadow: the memory of our friendship is the shadow that walks with me here: that seems never to leave me: that wakes me up at night to tell me the same story over and over till its wearisome iteration makes all sleep abandon me till dawn[34a]: at dawn it begins again: it follows me into the prison-yard and makes me talk to myself as I tramp round: each detail that accompanied each dreadful moment I am forced to recall: there is nothing that happened in those ill-starred years that I cannot recreate in that chamber of the brain which is set apart for grief or for despair: every strained note of your voice, every twitch and gesture of your nervous hands, every bitter word, every poisonous phrase[34b] comes back to me: I remember the street or river down which we passed, the wall or woodland that surrounded us, at what figure on the dial stood the hands of the clock, which way went the wings of the wind, the shape and colour of the moon.

有份报纸，我想是《泼尔穆尔报》吧，报道了我一出戏的彩排，说你像影子似的跟随着我：对你我友谊的回忆，就是在这里随我左右的影子，像是永不分离似的——深夜里唤我醒来，一遍又一遍地说着同一个故事，直磨得人睡意全无，醒到天明[34a]；天明时分又开始了，跟着我到牢房外的院子里，害得我一边步履沉重地走着一边喃喃自语——我被迫回想着每一个痛苦时刻的每一点细节，在那些个倒霉的年头里发生的事，没有哪一件我不能在那留给悲伤和绝望的脑室里再造重演：你每一点不自然的话音，每一个紧张兮兮的手势，每一句冷言恶语[34b]，都涌上了心头；我记着我们到过的街道和河流，四周的墙壁和树林，时钟的针正指着哪一点，风正吹向哪一面，月色月影又是什么模样。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。