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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Moreover, for all those possessions, from which anyone has, without the lawful judgment of his peers, been disseised or removed, by our father, King Henry, or by our brother, King Richard, and which we retain in our hand (or which as possessed by others, to whom we are bound to warrant them) we shall have respite until the usual term of crusaders; excepting those things about which a plea has been raised, or an inquest made by our order, before our taking of the cross; but as soon as we return from the expedition, we will immediately grant full justice therein.

此外，如有财产在我父亨利国王和兄理查德国王治下，未经同级贵族合法审判遭到剥夺，而该财产现属我们占有，或属应由我们提供保证者占有，则我们可按十字军战士所享宽限条件暂缓处理；除非在我们出征前相关诉讼已经提起，或已依据我们的令状开始审理；但是，一旦我们远征归来，或中断征程归来，则即应立刻按公平正义处理。注： a 、本条见于1215年最初的版本，后来版本无。b 、十字军战士可享有三年宽限期。

To achieve this, the or each steam generator pipe (22, 50, 60) has a respective downpipe section (34, 52, 62, 64), which is approximately vertical and through which the flow medium can flow downwards and a respective riser pipe section (36, 54, 66, 68) connected downstream of the downpipe on the flow medium side, which is approximately vertical and through which the flow medium can flow upwards.

为达到此目的，按本发明这一根或每根蒸汽发生器管(22、50、60)分别包括一个近似垂直布置可被流动介质沿向下的方向流过的下降管段(34、52、62、64)和一个沿流动介质流向连接在该下降管段下游的、近似垂直布置并可被流动介质沿向上的方向流过的上升管段(36、54、66、68)。

Had I continued in the Station I was now in, I had room for all the happy things to have yet befallen me, for which my Father so earnestly recommended a quiet retired Life, and of which he had so sensibly describ'd the middle Station of Life to be full of; but other things attended me, and I was still to be the wilful Agent of all my own Miseries; and particularly to encrease my Fault and double the Reflections upon my self, which in my future Sorrows I should have leisure to make; all these Miscarriages were procured by my apparent obstinate adhering to my foolish inclination of wandring abroad and pursuing that Inclination, in contradiction to the clearest Views of doing my self good in a fair and plain pursuit of those Prospects and those measures of Life, which Nature and Providence concurred to present me with, and to make my Duty.

我若能长此安居乐业下去，生活必然会无比幸福。正是为了能获得这些幸福，我父亲曾竭力规劝我过一种安份守己的平静生活；而且，他告诉我，只有中间地位的生活，才享有种种幸福。他的看法确实是通情达理、切合实际的。然而，冥冥中另一种命运在等待着我。我自己一手造成了自己的不幸，增加了自己的过错，使我后来回想起来倍加悔恨。我后来遭遇的种种灾难都是由于我执迷不悟，坚持我遨游世界的愚蠢愿望，并刻意去实现这种愿望。结果，我违背了大自然与造物主的意愿和自己的天职，放弃用通常正当的手段追求幸福的生活，以致给自己造成无穷的危害。

Had I continued in the Station I was now in, I had room for all the happy things to have yet befallen me, for which my Father so earnestly recommended a quiet retired Life, and of which he had so sensibly describ'd the middle Station of Life to be full of; but other things attended me, and I was still to be the wilful Agent of all my own Miseries; and particularly to encrease my Fault and double the Reflections upon my self, which in my future Sorrows I should have leisure to make; all these Miscarriages were pro by my apparent obstinate adhering to my foolish inclination of wandring abroad and pursuing that Inclination, in contradiction to the clearest Views of doing my self good in a fair and plain pursuit of those Prospects and those measures of Life, which Nature and Providence concurred to present me with, and to make my Duty.

我若能长此安居乐业下去，生活必然会无比幸福。正是为了能获得这些幸福，我父亲曾竭力规劝我过一种安份守己的平静生活；而且，他告诉我，只有中间地位的生活，才享有种种幸福。他的看法确实是通情达理、切合实际的。然而，冥冥中另一种命运在等待着我。我自己一手造成了自己的不幸，增加了自己的过错，使我后来回想起来倍加悔恨。我后来遭遇的种种灾难都是由于我执迷不悟，坚持我遨游世界的愚蠢愿望，并刻意去实现这种愿望。结果，我违背了大自然与造物主的意愿和自己的天职，放弃用通常正当的手段追求幸福的生活，以致给自己造成无穷的危害。

Had I continued in the Station I was now in, I had room for all the happy things to have yet befallen me, for which my Father so earnestly recommended a quiet retired Life, and of which he had so sensibly describ'd the middle Station of Life to be full of; but other things attended me, and I was still to be the wilful Agent of all my own Miseries; and particularly to encrease my Fault and double the Reflections upon my self, which in my future Sorrows I should have leisure to make; all these Miscarriages were pro by my apparent obstinate adhering to my foolish inclination of wandring abroad and pursuing that Inclination, in contradiction to the clearest Views of doing my self good in a fair and plain pursuit of those Prospects and those measures of Life, which Nature and Providence concurred to present me with, and to make my Duty.

我若能长此安居乐业下去，生活必然会无比幸福。正是为了能获得这些幸福，我父亲曾竭力规劝我过一种安份守己的平静生活；而且，他告诉我，只有中间地位的生活偶像拉丁文idola的意译。又译&幻像&或&假相&。详，才享有种种幸福。他的看法确实是通情达理、切合实际的。然而，冥冥中另一种命运在等待着我。我自己一手造成了自己的不幸，增加了自己的过错，使我后来回想起来倍加悔恨。我后来遭遇的种种灾难都是由于我执迷不悟，坚持我遨游世界的愚蠢愿望，并刻意去实现这种愿望。结果，我违背了大自然与造物主的意愿和自己的天职，放弃用通常正当的手段追求幸福的生活，以致给自己造成无穷的危害。

According to the said analysis and recent situation, it finally try to set up a risk management system in the general investment bank department in Southern Security Company, which is appropriate for the present financial environment in our country. In the design of independent matrix risk management system which makes risk supervision obtain comprehensive, controllable results, which puts risk under tight network system control, and which makes risk supervisors keep up with risk endurers from beginning to end, I describes the job content and duty in every position, investigate the operating way of the whole risk management system, make the duties of the full-time risk management perfect, and whole system.

根据上述分析，针对现有情况的问题，最后尝试为南方证券投资银行总部建立适合我国现有金融环境的风险管理体系，将投资银行的风险管理体系设计为独立的矩阵式风险管理系统，它使风险监管达到横向到边、纵向到底的效果，使风险置于纵横交错的网络式控制体系之下，使风险监管者与风险承受者保持始终的联系，不至于脱节。

At the end of March, our class organization must go to outdoors camp cooking each person to pay 100 Yuan, so-called is the return excess, we specially have also hired accountant ha, we must go the place is Beijing Huairou zone Tang Yunong the courtyard, we decided beforehand Friday embarks Saturday in the afternoon comes back, as soon as the preparation needs to live in the agricultural courtyard sleeps, the rent is in any case inexpensive,(lives in a late each talented person 10 Yuan), is inexpensive, because we rest is the ridge which puts,(did not know that you understand), the major activity is fishing, bakes, bakes inside this compared to fishing is interested, today weThe thing which "the collection" we need to outside, the thing which the fishing pole, the fish food and so on a series of fishing need, moreover we also bought several night light fishing to float, we prepared in the evening also to fish, stimulation ha…As for the thing which bakes we had decided that good will be this week buys next week buys, this week bought the meat words to fear that next week will roast has not been delicious, next week will buy does not fear the time, because our week 5 were on vacation from school embarks fundamentally has not had the time, therefore we very difficult to decide…This must be decided in our class "the board of directors" the decision, after having gone to result words that to let everybody and so on meet, because our in March 28 talents embark, must know that result that wanted the April's first week to issue diary again

三月底，我们班组织要去户外野炊每人交100元钱，所谓是多退少补吗呵呵，我们还特别雇佣了个会计员哈哈，我们要去的地点是北京市怀柔区城唐裕农家院，我们事先决定星期五出发星期六下午回来，准备要在农家院住一宿，反正租金也不贵，（住一晚每个人才10元），不贵吧因为我们睡的是北方的坎，，主要活动是钓鱼、烧烤，在这里面烧烤比钓鱼感兴趣，今天我们就到外面&搜集&我们所需要的东西，鱼竿、鱼食等一系列钓鱼所需要的东西，另外我们还买了几个夜光的渔漂，我们准备在晚上还要去钓鱼，刺激吧哈哈…至于烧烤的东西我们还没决定好是这星期买还是下星期买，这星期买了肉的话怕下星期烤了不好吃，下星期买又怕没时间因为我们星期5放学就出发了根本没时间，所以我们很难决定…这个吗还要取决于我们班里&董事会&的决定了，至于去了之后的结果的话那就要让大家等会了，因为我们3月28号才出发，要知道结果那就要4月的第一个礼拜再发布日志了

I soon found a way to convince him that I would do him no harm, and taking him up by the Hand laugh'd at him, and pointed to the Kid which I had kill'd, beckoned to him to run and fetch it, which he did; and while he was wondering and looking to see how the Creature was kill'd, I loaded my Gun again, and by and by I saw a great Fowl like a Hawk sit upon a Tree within Shot; so to let Friday understand a little what I would do, I call'd him to me again, pointed at the Fowl which was indeed a Parrot, tho' I thought it had been a Hawk, I say pointing to the Parrot, and to my Gun, and to the Ground under the Parrot, to let him see I would make it fall, I made him understand that I would shoot and kill that Bird; accordingly I fir'd and bad him look, and immediately he saw the Parrot fall, he stood like one frighted again, notwithstanding all I had said to him; and I found he was the more amaz'd, because he did not see me put any Thing into the Gun; but thought that there must be some wonderful Fund of Death and Destruction in that Thing, able to kill Man, Beast, Bird, or any Thing near, or far off; and the Astonishment this created in him was such, as could not wear off for a long Time; and I believe, if I would have let him, he would have worshipp'd me and my Gun: As for the Gun it self, he would not so much as touch it for several Days after; but would speak to it, and talk to it, as if it had answer'd him, when he was by himself; which, as I afterwards learn'd of him, was to desire it not to kill him.

其实，对星期五，我根本用不着采取任何防范措施。任何其他人都不可能有像星期五这样忠诚老实、听话可爱的仆人。他没有脾气，性格开朗，不怀鬼胎，对我又顺从又热心。他对我的感情，就像孩子对父亲的感情，一往情深。我可以说，无论何时何地，他都宁愿牺牲自己的生命来保护我。后来，他的许多表现都证明了这一点，并使我对此毫不怀疑。因此，我深信，对他我根本不用防备。

And thou shalt sanctify the breast of the wave offering, and the shoulder of the heave offering, which is waved, and which is heaved up, of the ram of the consecration, even of that which is for Aaron, and of that which is for his sons

29：27 那摇祭的胸和举祭的腿，就是承接圣职所摇的，所举的，是归亚伦和他儿子的。这些你都要成为圣

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。