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Through comparing exon sequences at both ends of introns of CHD genes of jungle fowls and cygnets, exons on two ends thereof are respectively designed with an upstream primer and a downstream primer: Psf: 5'CAAGGATGAGAAACTGTGCAA3'; and Psr: 5'GAATATCTTCTGCTCCTACTG3'; and the Psf/Psr primers are used for amplifying the CHD genes on Z and W chromosomes of meat pigeon feather pulp genome DNA, and finally the sex is identified according to the band type through 10 percent polyacrylamide gel electrophoresis and silver staining, wherein, female shows two bands and male shows one band.
通过原鸡和小天鹅CHD基因内含子两端的外显子序列比对,在两端外显子上分别设计上下游引物:Psf:5'CAAGGATGAGAAACTGTGCAA3';Psr:5'GAATATCTTCTGCTCCTACTG3';再以Psf/Psr引物扩增肉鸽羽髓基因组DNA的Z和W染色体上的CHD基因,最后经过10%聚丙烯酰胺凝胶电泳、银染,根据带型判断性别,其中雌性为两条带,雄性为一条带。
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I have been in all my Circumstances a Memento to those who are touch'd with the general Plague of Mankind, whence, for ought I know, one half of their Miseries flow; I mean, that of not being satisfy'd with the Station wherein God and Nature has plac'd them; for not to look back upon my primitive Condition, and the excellent Advice of my Father, the Opposition to which, was, as I may call it, my ORIGINAL SIN; my subsequent Mistakes of the same kind had been the Means of my coming into this miserable Condition; for had that Providence, which so happily had seated me at the Brasils, as a Planter, bless'd me with confin'd Desires, and I could have been contented to have gone on gradually, I might have been by this Time; I mean, in the Time of my being in this Island, one of the most considerable Planters in the Brasils, nay, I am perswaded, that by the Improvements I had made, in that little Time I liv'd there, and the Encrease I should probably have made, if I had stay'd, I might have been worth an hundred thousand Moydors; and what Business had I to leave a settled Fortune, a well stock'd Plantation, improving and encreasing, to turn Supra-Cargo to Guinea, to fetch Negroes; when Patience and Time would have so encreas'd our Stock at Home, that we could have bought them at our own Door, from those whose Business it was to fetch them; and though it had cost us something more, yet the Difference of that Price was by no Means worth saving, at so great a Hazard.
一般人往往有一种通病,那就是不知足,老是不满于上帝和大自然对他们的安排。现在我认识到,他们的种种苦难,至少有一半是由于不知足这种毛病造成的。患有这种病的人大可以从我的一生经历中得到教训。就拿我自己来说吧,正是由于我不满自己原来的境况,又不听父亲的忠告--我认为,我有悖教训,实为我的&原罪&,再加上我后来又犯了同样的错误,才使自己落到今天这样悲惨的地步。当时,造物主已安排我在巴西做了种植园主。如果我自己不痴心妄想发财,而是满足于逐渐致富,这时候我也许已成了巴西数一数二的种植园主了,而现在我却白白地在这荒岛上流落了这么多年,过着悲惨孤寂的生活。而且,我在巴西经营时间不长;就是在这段短短的时间里,我也获利不少。因此我确信,要是我继续经营下去的话,到现在一定拥有十几万葡萄牙金币的家财了。当时,我的种植园已走上了轨道,并且日益兴旺。可是,我偏偏把这一切丢弃,甘愿去当一名船上的管货员,只是为了到几内亚去贩卖黑奴。现在想来,我为什么要这样做呢?要是我守住家业,只要有耐心,经过一段时间之后,同样可以积聚大笔财富,我不是也可以在自己的家门口,从那些黑奴贩子手里买到黑奴吗?虽说价钱贵一点,但这点差价绝不值得自己去冒这样大的风险!
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A liquid cargo carrying maritime vessel including a plurality of liquid cargo carrying tanks and a plurality of gas expansion trunks wherein each gas expansion trunk is above, associated with, and in fluid communication with a respective tank therebelow, via slotted apertures in the deck.
一种携带液体货物的海洋船舶包括多个液体货物携带罐以及多个气体膨胀舱,其中各气体膨胀舱位于其下的各自罐的上方、经甲板内开槽的孔与之关联并流体连通。
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The tire cord fabric layer is provided with an edge wound around the core of the bead, and the wound part is provide with a base part and a radial outer part, wherein, the base part extends towards to the axial outer side from the inner side of the core of the bead along the core of the bead; and the radial outer part extends towards to inner side axially on the radial outer side of the core of the bead.
胎体帘布层具有绕胎圈芯缠绕的边缘,使得缠绕部分具有基部和径向外部,基部沿着胎圈芯从胎圈芯的轴向内侧延伸至其轴向外侧,并且径向外部在胎圈芯的径向外侧上轴向朝内延伸。
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The most famous book of the series.wherein four children step inside a wardrobe in England and emerge into the magical land of Narnia.a land complete with fauns and talking beavers.
它是系列中最有名的一本,英格兰某地的四个孩子走入一个衣橱,里面浮现出一个有魔力的纳尼亚大陆,这个大陆上有半人半羊的农牧神和会说话的海狸。
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Of the series,wherein four children step inside a wardrobe in England andemerge into the magical land of Narnia,a land complete with fauns and talking
孩子走进一个衣橱,里面浮现出一个有魔力的纳尼亚大陆,这个大陆上有半人半羊的农牧
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This series contains The Lion,The Witch and the Wardrobe,the most famous book of the series,wherein four children step inside a wardrobe in England and emerge into the magical land of Narnia,a land complete with fauns and talking beavers,a land under the rule and spell of eternal winter by the White Witch.
这个系列包含《狮子》、《巫婆》和《衣橱》,系列中最有名的一本,英格兰某地的四个孩子走进一个衣橱,里面浮现出一个有魔力的纳尼亚大陆,这个大陆上有半人半羊的农牧神和会说话的海狸,整个大陆处于由白魔女施下的永恒的冬天的规矩和符咒之下
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This series contains The Lion,The Witch and the Wardrobe,the most famous book of the series,wherein four children step inside a wardrobe in England and emerge into the magical land of Narnia,a land complete with fauns and talking beavers,a land under the rule and spell of eternal winter by the White Witch.
这个系列中有一本《狮子、女巫和魔法衣橱》,它是系列中最有名的一本,英格兰某地的四个孩子走进一个衣橱,里面浮现出一个有魔力的纳尼亚大陆,这个大陆上有半人半羊的农牧神和会说话的海狸,整个大陆处于由白魔女施下的永恒的冬天的规则和符咒之下。
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This series contains The Lion,The Witch and the Wardrobe,the most famous book of the series,wherein four children step inside a wardrobe in England and emerge into the magical land of Narnia,a land complete with fauns and talking beavers,a land under the rule and spell of eternal winter by the White Witch.
这个系列中有一本《狮子、巫婆和衣橱》,它是系列中最有名的一本,英格兰某地的四个孩子走进一个衣橱,里面浮现出一个有魔力的纳尼亚大陆,这个大陆上有半人半羊的农牧神和会说话的海狸,整个大陆处于由白魔女施下的永恒的冬天的规则和符咒之下。
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This series contains The Lion,The Witch and the Wardrobe,the most famous book of the series,wherein four children step inside a wardrobe in England and emerge into the magical land of Narnia,a land complete with fauns and talking beavers,a land under the rule and spell of eternal winter by the White Witch.
这个系列包含《狮子》、《巫婆》和《衣橱》,系列中最有名的一本,英格兰某地的四个孩子走进一个衣橱,里面浮现出一个有魔力的纳尼亚大陆,这个大陆上有半人半羊的农牧神和会说话的海狸,整个大陆处於由白魔女施下的永恒的冬天的规则和符咒之下。
- 相关中文对照歌词
- Wherein Lies Continue
- 推荐网络例句
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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.
索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。
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The relationship between communicative competence and grammar teaching should be that of the ends and the means.
交际能力和语法的关系应该是目标与途径的关系。
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This is not paper type of business,it's people business,with such huge money involved.
这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。