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Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

结果表明：利用电融合方法制备的小鼠四倍体胚胎在体外培养体系中经历细胞质融合、细胞核融合及细胞继续分裂发育直到囊胚期的过程，在细胞质融合的时候胚胎卵裂球同体内体外培养二倍体胚胎一样，呈现高度甲基化状态；在细胞核开始融合的时候，甲基化水平急速下降，在细胞核完全融合的时候甲基化水平达到最低点；随着胚胎继续分裂，胚胎甲基化水平逐渐增加，在囊胚期甲基化水平最高；但是囊胚期四倍体胚胎内细胞团同滋养层细胞甲基化荧光信号没有差别，这与体内体外培养二倍体囊胚内细胞团细胞甲基化荧光强度高于滋养层细胞甲基化荧光强度不同。

7Gm〓 in the 20 yr forest, 1139. 8gm〓 in the 80 yr forest and 912. 4 gm〓 in the climax forest, respectively. However, along with forest succession, the dominant tree species fine root annual production increases, whereas other arboreal and grass fine root annual production decreases.

白桦山杨幼林、白桦山杨成熟林与原始阔叶红松林中，细根年生长量分别为712.7gm〓、1139.8gm〓和912.4gm〓；但是，随着演替的进行，林地主要树种细根的年生产量会逐渐增加，其它木本和草本细根的年生产量则逐渐降低。

As a result of rapid development of city's industrialization in Shanghai whereas the lag of industrial and domestic sewage's disposal and the building progess of city's cloaca,most of rivers was polluted badly.

由于上海市工业化和城市化的迅速发展，雨污分流与污水处理的建设和发展相对滞后，致使大量工业废水和生活污水直接排入河道，河道水质严重恶化。

as a result of rapid development of city's industrialization in shanghai whereas the lag of industrial and domestic sewage's disposal and the building progess of city's cloaca,most of rivers was polluted badly.there are diversiform measures including biotechnologics for improving the water quality in shanghai.in this paper,the biotechnologic repairing's basic theory,effect,history,and application in the current world is expatiated.furthermore,a project sample which was put into practice in minghang district showed the feasibility and the actual effect of such technology in shanghai's water quality repairing.

由于上海市工业化和城市化的迅速发展，雨污分流与污水处理的建设和发展相对滞后，致使大量工业废水和生活污水直接排入河道，河道水质严重恶化。为改善上海河道水质，人们采取了多种措施，其中就包括采用生物技术修复河道水质的措施。本文浅要介绍了采用生物技术修复河道水质的原理、效果以及这种技术的发展简史、在国际上的应用现状，并以闵行区已经开展的邱泾河原位生物修复工程为例说明这种技术在上海市水质修复应用的可行性和实际效果。

Cloistered religious orders are called to a life of prayer and contemplation, whereas missionary orders are called to a life of compassionate, loving service.

cloistered宗教令是为了所谓的生活，祈祷和沉思，而传教的命令被称为一个生命的同情心，爱心的服务。

HM-Miner uses the upper bound property to cut down search space,whereas HM-Two-Phase-Miner uses transaction-weighted downward closure property to achieve the same goal.

HM-Miner利用激励的上界特性剪枝，HM-Two-Phase-Miner则利用事务权重激励向下封闭特性剪枝。

As regards rooting from clumpy buds, only NAA can induce rooting and NAA 0.5 mg/L is the best hormone concentration, whereas 6-BA has negative effect, and active carbon has no effect in this study.

猜A可使康乃馨愈伤组织分化，添加适量NAA效果更好，6-BA 1.0 mg/L＋NAA 0.5 mg/L和6-BA 2.0 mg/L＋NAA 0.5 mg/L诱导的芽点最多；但6-BA对丛生芽来源的无根苗的生根起抑制作用，活性炭对生根并无帮助，而以NAA 0.5 mg/L的生根效果最佳。

"I think there is a critical defect in this nominee, whereas if you are going to be charged with the health and the services of humans in this society, that you are confused on this very issue of transparency and accountability of taking the life of an unborn child," said Senator Coburn.

本人也是医生的科本说，这一任命有一个非常关键的错误。在有关剥夺未出生婴儿生命的问题中，涉及到透明度和承担责任的问题。而正是在这个问题上，负责健康卫生和服务于人的官员本身却不明是非。

One of the most common ways to describe a wood's tonal properties is in terms of its frequency range,which is often broken down into low-end frequencies,mid-range and high-end frequencies.picture it as a visual spectrum,as we've done in the chart to the right,with the lower frequencies on the left and the higher frequen-cies on the right.the graph line for each wood visually depicts its general tonal range.rosewood and ovang kol,for example,tend to resonate with more low-end frequencies,whereas koa,cocobolo and maple tend to sound brighter from having more top-end frequencies.note also rosewood's"scooped"midrange and ovang-kol's fuller midrange.the doted lines for walnut and koa denote the expansion of low-end frequency range as the guitar opens up after a period of playing it.

最常见的方式描述了木材的声音特性是在其频率范围内，这往往是细分为低端频率，中端和高端frequencies.picture它作为一个视觉频谱，因为我们'维生素E这样做的图表，以权，以较低的频率在左边和更高的频率，种上right.the图线的每一个木材视觉描绘的一般音调range.rosewood和ovang科尔举例来说，往往会产生共鸣更多的低端频率，而兴亚， cocobolo和枫树往往从健全光明有更多的高端frequencies.note也花梨木的"收购"的中端和ovang -科尔的充分midrange.the doted线的核桃和兴亚指扩大低端频率范围内的吉他开辟了一段时间后，打它。

A holoenzyme is the catalytically active form of the enzyme with its cofactor,whereas an apoenzyme is the protein part on its own.

全酶是酶与辅助因子一起形成的催化活性形式，而辅基酶本身的蛋白质部分。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。