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A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

The word "realism" in the West includes the foregoing two aspects of connotation, depending its specific meaning on the described content, whereas these two aspects are described as the "factualism" and "realism" respectively in Chinese of course, these two terms are related somehow, for the realistic art is embodied by the factualistic approach, whereas other arts in the form or with the language of symbolism, nonobjectivism or absurdism are often included into other isms although such arts demonstrate the realistic traits and essence.

这两层意思在西文中都用一个Realism来表示，根据它所述内容而确定其含义，而在中文里则用"写实主义"与"现实主义"分别来加以表达。

The day passed thus; he scarcely tasted food, but walked round and round the cell like a wild beast in its cage. One thought in particular tormented him: namely, that during his journey hither he had sat so still, whereas he might, a dozen times, have plunged into the sea, and, thanks to his powers of swimming, for which he was famous, have gained the shore, concealed himself until the arrival of a Genoese or Spanish vessel, escaped to Spain or Italy, where Merc d s and his father could have joined him. He had no fears as to how he should live--good seamen are welcome everywhere. He spoke Italian like a Tuscan, and Spanish like a Castilian; he would have been free, and happy with Merc d s and his father, whereas he was now confined in the Chateau d'If, that impregnable fortress, ignorant of the future destiny of his father and Merc d s; and all this because he had trusted to Villefort's promise.

这一天就这样过去了，他没吃一点食物，只是在斗室里走来走去，象一只被困在笼子里的野兽似的，最使他苦恼的是，在这次被押送的途中，他竟这样的平静和呆笨，他本来这次跳海也是成功的，他的游泳技术是素来有名的，他可以游到岸边躲起来，等到热那亚船或西班牙船来的时候，逃到西班牙或意大利去，美塞苔丝和他的父亲可以到那儿去找我团聚，他跟本用不着担心以后的生活，因为他是一个好海员是到处都受人欢迎的，他讲起意大利语来就象托斯卡人一样［意大利的一种民族。

The CPT values in the affected leg in patient group were significant higher than those in control group at all frequencies. The CPT values in contralateral leg in patient group were significant higher than those in control group at 5Hz whereas there were no significant differences at 250Hz and 2000Hz. The CPT values in affected leg in patient group were significant higher than those in contralateral leg at 250Hz and 2000Hz whereas there were no significant differences at 5Hz. All the CPT values showed the same linear decrease from the dermatome of the compressed root to the dermatomes of the contralateral neighbor dematom.

在用CPT监测8例腰骶单神经根病组患侧和健侧及与正常对照组的不同直径感觉神经纤维功能的研究中发现压迫皮节和其临近皮节的所有频率CPT值无任何显著性差别；患者压迫侧的2000Hz及250HzCPT值及压迫侧和对侧的5HzCPT值与正常对照组都有显著性差别；患者组5HzCPT值患侧和对侧无差别，其他两组均有显著性差别；不管有无显著性差别，神经根病患者组各皮节三频率CPT值均按照压迫皮节，临近皮节，压迫对侧皮节，压迫对侧临近皮节的顺序减小，说明在腰骶单神经根病中Aβ纤维及Aδ纤维及无髓C纤维均受累。

We proposed this SNP site correlated with IMP content in chickens. A neighbour-joining dendrogram was constructed based on the Nei's genentic distance. The genetic relationship between Chinese red jungle fowl and Tibetan chickens was the nearest, whereas Baier chickens were more closer to Silkies chickens. The Chinese red jungle fowls were relatively closer to the domestic fowls, whereas Thailand red jungle fowls were relatively diverging to the Chi-nese native breeds. These results supported the theory concerning the independent origins of Chinese native fowl breeds.

根据该多态位点的基因频率，基于Nei氏的遗传距离运用NJ聚类法构建系统发生树，进行家鸡与原鸡的亲缘关系分析，结果发现，丝羽乌骨鸡与白耳鸡的亲缘关系最近，藏鸡和中国红原鸡亚种的亲缘关系也较近，中国地方家鸡品种与中国红原鸡亚种的亲缘关系较近，而与泰国红色原鸡的亲缘关系较远，隐性白羽鸡与原鸡亲缘关系最远，初步得出中国家鸡有自己独自的血缘来源的结论。

To reflect that each one who enters imagines himself to be the first to enter whereas he is always the last term of a preceding series even if the first term of a succeeding one, each imagining himself to be first, last, only and alone whereas he is neither first nor last nor only nor alone in a series originating in and repeated to infinity.

649他仔细一想，每一个进入者都认为自己是头一个进去的，其实，他总是一连串先行者的后继者，即便他是一连串后继者的第一个。每个人都自以为是头一个，最后一个，唯一的和独一无二的，其实在那源于无限，又无限地重复下去的一连串当中，他既不是头一个，也不是最后一个，既不是唯一的，也不是独一无二的。

The results showed that:Under NaCl stress,the reductions in the shoot and root dry weight, leaf area,stem diameter of grafted plant were lower than those of ungrafted plants.The Na~+ content,Na~+/K~+ ratio,Na~+/Ca~(2+) ratio in the leaf and scion stem of grafted plants were lower whereas those of in the rootstock stem ad root were higher than in ungrafted plants. The K~+ contents,soluble sugar contents,shoot water contents,SOD,POD,CAT,APX activity,the stability of AsA/DHA,Photosynthetic rate,transpiration rate and stomatal conductance were higher,whereas the MDA contents in the leaf of grafted plants were lower than that in ungrafted plants.The reductions in leaf area and stem diameter of grafted \'Jinchun No.2\' plants were more severe than those of grafted \'Zaoduojia\' ones.

结果表明，盐胁迫下，嫁接苗地上部和根干重、叶面积、茎粗降低的程度显著低于自根苗；嫁接苗叶片和接穗茎中Na~+含量和Na~+/K~+、Na~+/Ca~(2+)比值显著低于自根苗，而砧木茎和根中的Na~+含量和Na~+/K~+、Na~+/Ca~(2+)比值显著高于自根苗；嫁接苗叶片中K~+含量、可溶性糖含量、地上部含水量、SOD、POD、CAT、APX活性、AsA/DHA的稳定性、光合速率、蒸腾速率、气孔导度显著高于自根苗，MDA含量显著低于自根苗。

The results showed that:The reductions in the shoot and root dry weights, leaf area,stern diameter of grafted plants were lower than those of ungrafted plants under the same NaCl stress.Na~+,MDA,H_2O_2 contents,O_2~- producing rate,Na~+/K~+ ratio, Na~+/Ca~(2+) ratio were lower,whereas K~+,solube sugar,shoot water content,SOD,POD, CAT,APX activity,the stability of AsA/DHA,Photosynthetic rate,transpiration rate and stomatal conductance in the leaf of grafted plants were higher than those of ungrafted plants at the same NaCl stress.Intercellular CO_2 concentration of grafted plants decreased whereas that of ungrafted plants increased significantly under salt stress.

结果表明，盐胁迫下，嫁接苗地上部和根干重、叶面积、茎粗受抑制的程度显著低于自根苗；嫁接苗叶片中Na~+、MDA、H_2O_2含量、超氧阴离子产生速率、Na~+/K~+和Na~+/Ca~(2+)比值显著低于自根苗，而叶片中K~+含量、可溶性糖含量、地上部含水量、SOD、POD、CAT、APX活性、AsA/DHA的稳定性、光合速率、蒸腾速率、气孔导度显著高于自根苗；嫁接苗的胞间CO_2浓度显著下降，自根苗的则显著上升。

It is shown that polystyrene has been grafted on the surface of nmSiO2 particles and the grafting ratio is about 40%. The surface of nmSiO2 could be treated effectively by KH570in absolute ethanol at 25℃, 48h. The nmSiO2-g-PSt unpurified by toluene appear microsphere in which nmSiO2 particles are contained, whereas the purified one appear internet formed by many pieces. The grafting ratio of PSt on nmSiO2 surface could be influenced obviously by dispersion agents content and agitation speed, whereas the morphology of nmSiO2-g-PSt purified is rarely changed.

结果表明：常温下在无水乙醇介质中处理48h以上可获得良好的硅烷处理效果；PSt已接枝包覆于nmSiO2表面，接枝率约40%；经甲苯洗涤前nmSiO2-g-PSt呈多粒子包覆微球状，洗涤后呈单粒子包覆网状结构；分散剂用量、搅拌速度改变对接枝率有影响，但对包覆形态影响不大。

Whereas in post-Napoleonic Germany publicity as the organizational principle of a liberal constitutional state found its first eloquent champions, and whereas at the time Welcker and Feuerbach advocated publicity in the parliament and in the judiciary in conjunction with a freely developing, critically debating political daily press, one is concerned today to shield parliamentary deliberations and judicial processes from a plebiscitary public.

德国议会上院已经建议不再直播议会例会；刑事律师和法官甚至更强烈的要求不惜使用一切法律手段，（如果还不能奏效，就改变司法程序，）来阻止广播和电视对庭审作现场报道。在两种情况中，公开性的原则可能减至对'亲自到场的公众准入性'的最低保证。

But this is impossible, as long as it is engaging in a market economy, there are risks in any operation.

但是，这是不可能的，只要是搞市场经济，是有风险的任何行动。

We're on the same wavelength.

我们是同道中人。