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In this study,we focus on the interaction between nuclear modifier gene MTO2 and aminoglycosides,and have received the following innovations.1,We here report first that the knockout of MTO2 significantly inhibited mtDNA p_(454)~R mutant's sensitivity to aminoglycoside antibiotics.Here are the results:(1)In neomycin GYP medium,when mitochondria genes were wild type,whether MTO2 knockout or not,the growth of yeast strains has no significant difference;when mitochondria genes were complete deletion,whether MTO2 knockout or not,the growth of yeast strains also has no significant difference;Interesting,when mtDNA P_(454)~R,wild-type MTO2 strains were hardly survival in neomycin GYP medium,but the growth of MTO2 knockout strains were only partly inhibited,there was a significant difference performance of neomycin sensitivity between them.

具体结果如下：(1)在含有新霉素的葡萄糖培养基上，mtDNA为野生型、MTO2敲除与否，相应的酵母菌株的生长没有显著差异；mtDNA完全缺失、MTO2敲除与否，相应的酵母菌株的生长也没有显著差异；然而，当mtDNA存在P_(454)~R突变时，MTO2野生型菌株几乎完全不能生长，而MTO2敲除菌株的生长只受到部分抑制，表现二者对新霉素的敏感性存在显著差异。

The rain is knockin at my heart and I can't see the sun no more I can't live without you my sun if I'm ever gonna get back picture this You got a kid in a room his hands sweatin and shakin while he's lacin his shoes the excitement's buildin up for what's comin up soon To get there and do what he was born to do He's listenin to the beats He's trying to get in the zone He's gotta take control and make every moment his own just a little bit tired he is a little bit scared but he's so focused he won't show it when he's finally there Cuz now he's out on the floor And he's catchin the pass slashin to the glass, movin so fast, right to the hole, they don't know how to stop him and he keeps on droppin 2's and 3's by the second and the crowd is rockin so they knock him knock him down to the ground before he's crowned as the best He takes a deep breath before he gets up and looks around Everything's in slow motion The crowd is an ocean Only one minute left The rain is knockin at my heart and I can't see the sun no more I can't live without you me sun if I'm ever gonna get back, get back, get back The rain is knockin at my heart and I can't see the sun no more Ican't lice without you my sunif I'm ever gonna get back Imagine A little girl in the stands the kid's her older brother and she's his number one fan He's always lookin out for her and she looks up to him and she wants nothin more than for her brother to win But the truth is that there's someone on the other team Someone who stole her heart Someone who holds her dreams They kiss for the 1st time just the other evening Her brother's 23 but her lover's number 16 The drama is set the whole scene is intense she doesn't know which one to cheer for and she's gettin upset 23 on the chase cuz they're at each other's necks and what happens next Her boyfriend steals the ball and checks the kid in the chest 16 on the break Brother goes in the air with brother up in his face They come crashing to the dirt Tears about to burst When the game is over who she gonna run to first, yo The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back, get back, get back The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back 有时感觉阻碍太多但我还是为了我的梦想而奋斗 Nothin's gonna stop me Ey yo this game of life is one big struggle We gotta hustle just to make it everyday to fight the fears we facin and grab those dreams we chasin cuz there ain't no competition when we rise to the occasion c'mon The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back, get back, get back The rain is knockin at my door and I can't see the sun no more I'ma hafta fight my way if I'm ever gonna get back 注:IN可以看作是ING如FACIN可以看作facing

雨是敲入我的心我看不到太阳不再我不能没有你我的太阳如果我曾经去买回来此图片你有一个孩子在一个房间里他的手sweatin和沙金，而他的拉欣他的鞋兴奋的buildin了什么的，很快就要来要到那里做什么，他出生做他listenin的节拍他试图在该地区获得他得采取控制和利用自己的每一刻只是有点累了他有点害怕但他是如此专注，他将不显示它的时候，他终于有现在的Cuz他在地上他的捉通行证 slashin的玻璃，居无定得那么快，有权向孔，他们不知道如何阻止他他不断droppin 2的和3的由第二摇滚和人群所以他们会把他会把他撞倒在地之前，他的加冕为最佳他深吸了一口气，才起身环顾四周一切都在慢动作人群是一个海洋仅1分钟雨是敲入我的心我看不到太阳不再我不能没有你我的太阳如果我曾经去买回来，回来，回来雨是敲入我的心我看不到太阳不再没有你我的sunif Ican't虱子我曾经去买回来想像看台上的一个小女孩这个孩子是她的哥哥，她是他的头号球迷他总是在找谁了她和她抬起头来他和她想没什么比她的哥哥更赢得但是，事实是，对其他团队的人有人谁偷了她的心有人谁拥有她的梦想他们只是亲吻对方的第一时间为晚上她的弟弟，但她的爱人23的16号该剧设置整个场面激烈她不知道哪一个加油，她的gettin不高兴 23大通他们的Cuz在对方的脖子和接下来会发生什么她的男朋友抢断球，并检查孩子的胸部 16个突破哥哥和弟弟去他的脸在空中他们来到崩溃的污垢即将爆裂泪当比赛结束谁，她要去跑第一，溜溜雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来，回来，回来雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来有时感觉阻碍太多但我还是为了我的梦想而奋斗没办法，拢不惊安永哟这样的生活游戏是一个大斗争我们得喧嚣这只是为了日常战斗的恐惧，我们都会面临的两个并抓住那些梦想，我们查辛没有的Cuz没有竞争当我们挺身而出来吧雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来，回来，回来雨是敲着我的门我看不到太阳不再我是打我的方式hafta 如果我曾经去买回来

And when I see a hard-bitten lawyer happily planning an outing for blind children; when I watch men of great prestige and wealth put their efforts into brotherhood; when I observe men and women begin to grow out of their own little tiny shells by going into the world and maturing by knowing other peoples, other greater sorrows, other new shared joys; then I know that this is worth believing in.

当我看到一个顽强的律师带着一群失明的孩童出去郊游，当我看到拥有富贵和权耀的人致力于帮助自己的同胞时，当我看到越来越多的男男女女走出自己的小屋，试着接触越来越多的陌生人的悲喜，我知道这一切是值得相信的。

And when I see a hard-bitten lawyer happily planning an outing for blind children;when I watch men of great prestige and wealth put their efforts into brotherhood;when I observe men and women begin to grow out of their own little tiny shells by going into the world and maturing by knowing other peoples,other greater sorrows,other new shared joys;then I know that this is worth believing in.

当我看到一个顽强的律师带着一群失明的孩童出去郊游，当我看到拥有富贵和权耀的人致力于帮助自己的同胞时，当我看到越来越多的男男女女走出自己的小屋，试着接触越来越多的陌生人的悲喜，我知道这一切是值得相信的。暂无

Although when we gone through the first polishing, it is already a sight to behold, hard-edged, but when we become a time when people have the ability, but also easy to develop a conceited, arrogant, irascible and vain character, as well as in the Life was all obstacles.

虽然当我们经过了第一次的打磨，便已经超凡脱俗、锋芒毕露了，但是当我们变成了一个有能力的人的时候，却也容易养成自负、傲慢、暴躁和虚荣的性格，以至在生活中被大家设置障碍。

He sucked mountain stream when he felt thirsty,and laid in the hayrick when he was tired.when the loneness came,he sang he folk songs.

渴了，就喝溪水；累了，就躺在草堆里打个盹；寂寞了，就唱唱山歌。

When Elizabeth broke with the Catholic bishops who would not acknowledge her spiritual headship, and when William III deprived Sancroft and his suffragans who refused the oath of allegiance, a test was applied, dogmatic in 1559, perhaps not less so in 1690, which proves that no cause of exemption can be pleaded against the king when he acts as supreme governor of the Church.

当黄钱其濂打破了天主教主教团谁不承认她的精神校长，当威廉三世剥夺桑克罗夫特和他的suffragans谁拒绝宣誓效忠，测试，应用，教条式的在1559年，也许不是那么在1690年，这证明没有事业的豁免可以认罪，对国王时，他作为最高总督的教会。

When we were in frustration of dealing with the problems, when we were to fall apart in disasters; when we were in despair of being betrayed; we may tackle those with 'heartsease' --- thanks to the God, we will be given a new life, a renascence of hope in mind.

当面对困难我们认为解决无望时，当面对挫折我们就要倒下时，当面对背叛我们无能为力时，我们以感恩的心面对这一切，我们就会获得重生的机会，让希望再次出现在我们的生命中。

I remember that when I left school I said to myself:"Henceforward I can get up when I like and go to bed when I like."

我记得我在离开中学时曾对自己说过："从今以后，我可以高兴什么时候起来就什么时候起来，高兴什么时候上床睡觉就什么时候上床睡觉了。"

When n/n=0, the product is the mixture of h-WO3 (hexagonal system WO3) and WO3.1/3H2O, and have a morphology of nanorods; when n/n=0.5 or n/n=l, the product is γ-WO3, and have a morphology of rectangular flakes; when n/n=2, the product is β-WO3, with the same morphology of rectangular flakes.

当n/n=0时，产物为h-WO3和WO31/3H2O组成的混合物，形貌为纳米棒；当飞n/n=0.5和n/n=1时，产物为γ-WO3形貌为矩形片；当n/n=2时，产物为β-WO3，形貌同样为矩形片。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐