查询词典 western

The expression levels of Bcl-2 protein were assayed by Western-blot and cell proliferation was measured by MTT method.

通过倒置荧光显微镜和流式细胞仪观察细胞的转染效率，Western印迹法检测Bcl-2蛋白表达水平，MTT法测定细胞增殖情况。

Western blot assays were used to detect the expression of βNGF.

Western Blot检测蛋白的表达情况。

Furthermore, the expression of bFGF and angiogenin was examined by RT-PCR and Western blot assays.

接着用RT-PCR和Western blot的方法检测了bFGF和血管生成素的表达情况。

The gene expression of orexin in rat hypothalamus tissue was examined by real-time PCR and Western blooding analysis.

应用实时定量PCR和Western 印迹杂交分析检测下丘脑组织中orexin的mRNA和蛋白质在急性低血糖应激状态下的表达改变。

The effects of different renaturation agents, blockings and labelled conjugates were studied to improve the technique of non-radioactive Western Blot.

应用不同蛋白质复性剂、封闭剂和标记物的对比方法以探讨Western印迹技术的改进。

Coli BL21 (DE3) plysS for expression under induction of IPTG. The expressed product in a form of inclusion body was denaturalized with urea, purified by nickel ion chelate affinity chromatography, renaturalized by gradient dialysis and identified by Western blot.

coli BL21(DE3) plysS进行诱导表达，采用Ni(上标 2+)螯合亲和层析法纯化经尿素变性的包涵体，梯度透析复性纯化的目的蛋白，并进行Western blot鉴定。

In this study, we cloned the BFV gag gene into prokaryotic expression vector pET28a and purified the denaturalized Gag protein. The protein was used to immunize BALB/c mouse to produce antiserum, which could specifically recognize the BFV Gag protein in BFV-infected cells through western blot assay.

本文将BFV gag基因克隆入原核表达载体pET28a中，纯化得到了变性的Gag蛋白，用于免疫BALb/c小鼠获得抗血清，进行了Western blot杂交和免疫荧光实验。

Methods The total RNA was acquired from young rabbit particular cartilage, the BMP7 gene was inserted into pcDNA-3.1 to construct eukaryotic expression vector plasmid of pcDNA3.1-BMP7; the target gene-BMP7 was identified respectively by PCR analysis, restriction enounces analysis and nucleotide sequencing; the plasmid was transfected into adult rabbit knee articular chondrocytes, then the expression of BMP7 was detected by in situ hybridization, PCR and Western blotting.

从体外培养的幼兔膝关节软骨细胞中提取总RNA；按GenBank BMP7基因序列化学合成2条引物，采用RT-PCR方法得到BMP7基因；将BMP7基因片段插入到真核表达载体pcDNA 3.1中，构建pcDNA3.1-BMP7真核表达载体质粒；利用双酶切、PCR及核苷酸序列分析鉴定BMP7目的基因；将重组pcDNA3.1-BMP7真核表达载体质粒转染家兔关节软骨细胞，分别采用原位杂交、PCR、Western blotting法测定BMP-7表达。

Sheep were immunized with DNA vaccine and antibody production was analyzed by western blot and ELISA.

将纯化的DNA疫苗免疫绵羊后，用western blot和ELISA方法检测疫苗在绵羊体内的翻译和诱导IgG的产生。

Based on establishment of the human macrophage-derived foam cell model, the expression of Kv1.3 channel was investigated by immunocytochemical staining, reverse transcription-polymerase chain raction and Western blot. Furthermore, the effects of rMargatoxin, a Kv 1.3 channel-specific inhibitor, on cholesterol metabolism in macrophages incepting oxidized low density lipoprotein were studied.

在建立人巨噬细胞源性泡沫细胞模型的基础上，采用免疫细胞化学染色法、逆转录聚合酶链反应(transcription-polymerase chain raction，RT-PCR)及Western blot，研究Kv1.3通道的表达，并观察其特异性阻断剂--rMargatoxin对摄取氧化修饰低密度脂蛋白的巨噬细胞胆固醇代谢的影响。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新