查询词典 western

Blood and CSF of patients were collected for syphilis,toxoplasma gondii and routine,cytological, glycose,protein,chloride, virology examination and 14-3-3 protein Western Blot of CSF were were carried out too.

采集患者血液和CSF，进行CSF常规及细胞学、糖、蛋白质和氯化物；血液和CSF病毒学、弓形体、梅毒检测；CSF 14-3-3蛋白Western Blot检测。2例死亡患者脑组织匀浆进行Wistar大鼠脑内接种，观察期6个月。

Using cytochalasin D to prevent global actin from forming filamentous structures, the production of Heliothis armigera nuclear polyhedrosis virus in Hz-AM1 cells grown in the medium containing 0.5μg/ml CD was completely inhibited while its production was reduced to 10〓 in the presence of 0.1μg/ml CD by plaque assay. However, Western blotting revealed that the actin quantity in infected host cells treated or untreated with CD was almost identical. Additionally, CD had no effect on the DNA synthesis of HaNPV.

Western blot表明细胞松弛素D并不影响受染细胞中肌动蛋白的含量，Dot blot表明病毒DNA的合成也没有受到影响，但电子显微镜超薄切片发现在0.5ug／mL细胞松弛素D中形成的病毒粒子形态与正常的有明显区别，认为细胞松弛素D抑制棉铃虫核型多角体病毒的增殖是因为抑制病毒的DNA和蛋白组装成完整有感染性的病毒粒子，即丝状肌动蛋白对病毒粒子的脱壳和DNA的复制、转录没有影响，但对病毒粒子的组装是必须的。

Western blot analysis indicated that all of the fusion proteins were expressed in AO3_ 1 cells, in which multiple copies of the lac operator were integrated to produce a heterochromatic region of the genome.

Western blot检测表明，这些重组质粒分别转染AO3_1细胞后均表达了相应的融合蛋白。

The specificity of polyclonal antibody was detected with Western blotting, which applied rabbit antiserum and sera of known Humulus pollen allergic patients.

同时对葎草花粉过敏性哮喘患者血清进行Western blotting检测。

Western-blot analysis and indirect immunofluorescence assay showed that the gp90 protein expressed successfully was 45ku in molecular weight,possessed good reactionogenicity and could be recognized by mouse hyperimmune sera against the gp90 protein under natural or denatured state.

抗gp90蛋白小鼠超免疫血清介导的Western-blot分析和间接免疫荧光试验结果显示，gp90蛋白被正确表达，分子质量约为45ku；gp90蛋白在变性和自然状态下均能够被识别，具有良好的生物活性。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Immunofluorescence staining and western blotting indicated that hyphal fragments also enhanced the expression of TLR2 and TLR4 in protein level.

荧光免疫组织化学和western blot结果显示灭活菌丝片断能促进TLR2和TLR4蛋白水平的表达，而且这种表达能进一步被氢化可的松上调。

The recombinant plasmids were analyzed by sequencing and the one containing the correct fragment was transfected into K562 cells. The condition of idiotypic protein expression was tested by Western-Blot.

将经测序鉴定正确的重组质粒转染K562细胞，Western-Blot等方法检测其蛋白表达的情况。

The Western blot experiment showed it was immunogenic.

Western blot分析结果证实该重组蛋白具有良好的免疫原性。

The expressed His-fusion protein was shown to be immunologically active by Western blotting.

经ELISA和Western Blotting检测，表达的重组蛋白具有免疫学活性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新