查询词典 western

MMP-3 proenzyme protein expression in the different stages of liver fibrosis was measured by half quantitative analysis of Western blot.

建立四氯化碳中毒性大鼠肝纤维化模型，采用半定量Western免疫印迹方法，对肝纤维化不同阶段肝内MMP - 3的酶原蛋白的表达水平进行了检测。

Experimental liver fibrosis; stromelysin-1; proenzyme protein expression; Western blot

实验性肝纤维化；基质分解素-1；酶原表达； Western印迹法

Marked changes of apoptosis including condensation of chromatin and nuclear fragmentation were observed very clearly by Hoechst 33258 fluorescence staining and a characteristic "ladder" of DNA fragments was elicited by agarose gel electrophoresis; Western blot analysis revealed that caspase-3 was activated by the loss of caspase-3 proenzyme (32kDa) and the appearance of its 20kDa subunit, and that along with the apoptotic process caspase-3 activity was increased concurrently.

Hoechst 33258荧光染色观察到典型的核浓缩、核碎裂等细胞凋亡的形态学变化，琼脂糖凝胶电泳观察到细胞凋亡时的DNA"梯形"条带；Western blot检测结果表明32kDa的caspase-3酶原被激活，出现20kDa的亚单位活化片段，同时在细胞凋亡过程中，caspase-3活性显著增高。

The effect of bavachin and corylin, two active principles from Psoralea corylifolia L., on UMR106 cyclin E expression by Western blotting was examined. The result showed that the two flavonoid compounds had no significant effect on cyclin E expression after various exposure time.

对于中药补骨脂中分离的两个促成骨样细胞增殖的活性成分补骨脂二氢黄酮和补骨脂异黄酮，采用Western blotting的方法考察不同时间对UMR106细胞周期蛋白cyclin E的表达，结果它们对cyclinE的表达没有显著影响。

Methods Mouse macrophages were stimulated with Mpe LAMPs to analyze the activitation of NF-κB, and the effect of pyrrolidine dithiocarbamate, an inhibitor of NF-κB, on NF-κB activation was also studied by indirect immunofluorescence and western blot.

用从穿透支原体中提取的脂质相关膜蛋白刺激小鼠巨噬细胞，经Western blot和间接免疫荧光等方法检测NF-κB是否被激活，以及NF-κB抑制剂PDTC对NF-κB活性的影响。

Methods Forty women and their babies (40) were enrolled in this study. Placental tissues were assayed for leptin mRNA by reverse transcription/polymerase chain reaction, and assayed for the obese gene protein leptin by Western-blot and immunohistochemistry. Blood was taken from the umbilical cord of the babies at delivery. Serum leptin was measured by radio-immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery.

方法在40例产妇分娩时采集胎盘与脐血，采用逆转录聚合酶链反应检测胎盘肥胖基因mRNA相对表达水平，采用Western-Blot检测胎盘肥胖基因蛋白表达水平，采用免疫组化观察胎盘肥胖基因蛋白表达位点，采用放射免疫法检测脐血瘦素水平，采用Ponderal指数［PI=100×体重/身长3］估测新生儿营养状态。

Citri had been selected by ribosome display in previous research. In order to express the scFvs with bio-activity efficiently, the genes of the selected scFvs were transformed into Escherichia coli HB2151 for expressing resoluble antibodies. The expression levels of scFvs were detected by dot blotting and Western blotting, and the expressed scFvs were purified by affinity chromatography, and then the activity of the purified scFvs was tested by enzyme-linked immunosorbent assay .

Citri， Xac高亲和力单链抗体(Single chain variable fragment， scFv)GX13、GX44和GX95，为高效表达具有生物活性的单链抗体，本研究将前期筛选的高亲和力单链抗体基因转入大肠杆菌HB2151中进行可溶性表达，点印迹和Western印迹检测可溶性单链抗体的表达水平，亲和层析纯化表达的单链抗体，酶联免疫吸附法检测纯化单链抗体的抗原结合活性。

Change its BL21 bacterium, with IPTG revulsive its convey corresponding protein, use Western Blotting test and verify.

将其转化BL21细菌，用IPTG诱导其表达相应的蛋白质，并用Western blotting验证。

The expressions of RhoA, ROCK1/2 and COL Ⅱ in the induced BMSCs and after application of enzyme inhibitor Y27632 were detected by Western-blot and BT-PCR.

采用Western-blot和RT-PCR方法分别检测RhoA和ROCK1/2Ⅱ型胶原在细胞分化过程中的表达及应用Rho激酶抑制剂Y276532后的表达。

Methods Mouse models of tibia fracture healing were established, and callus samples were collected 1, 3, 7, 14, 21 and 28 days after fracture. The development of callus and new bone formation were evaluated with roentgenology, Micro-CT and tetracycline double labeling method, and the expression of HIF-1α, vascular endothelial growth factor, Runx2 and ALP in callus were detected with RT-PCR, Western blotting and immunohistochemistry. The relationship between HIF-1α and fracture healing was analysed.

建立小鼠胫骨骨折模型，骨折后1、3、7、14、21、28 d取材，应用X线摄片、Micro-CT和四环素荧光双标记技术，观测骨痂组织的演变和新骨形成状况；采用RT-PCR、Western blotting和免疫组织化学方法，检测骨痂组织细胞HIF-1α、血管内皮生长因子和成骨性标志物（Runx2和ALP）的表达状况，分析HIF-1α与骨折愈合进程的关系。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。