查询词典 western-style

In the paper, a preliminary study was made to analyze the interaction between hemocyanin, which was purified from shrimp P. vannamei obtained from Shantou, and anti -human antibody. The methods of Sephadex G-75 column chromatography, PAGE, Dot-ELISA, SDS-PAGE and Western-blotting were used.

以采自汕头的南美白对虾血清为研究对象，运用Sephdex-75柱层析、PAGE、Dot-ELISA、SDS-PAGE和Western-blotting等方法，探索血蓝蛋白与抗人IgG之间的相互作用。

Methods: Human colon cancer cell line DLD1 was treated with Bcl-XL siRNA, The protein level of Bcl-XL in the cells was determined by Western blot; the apoptotic ratio and survival condition were detected by flow cytometry assay and trypan blue-stained cell counting with hemocytometer, respectively. Results: The level of Bcl-XL protein in DLD1 cells was obviously down-regulated by Bcl-XL siRNA.

采用人结肠癌细胞株DLD1，利用人工合成的Bcl-XL靶向小分子干扰RNA转染DLD1细胞，通过Western blot法检测转染细胞Bcl-XL蛋白的表达，流式细胞仪检测处理后细胞的凋亡情况，锥虫蓝染色法计数存活细胞。

Western blot analysis showed that MdLys was highly expressed in midgut and was also detected in the hemolymph and fat body.

在蛋白水平上通过Western Blot 分析发现，该基因在中肠中大量表达，在脂肪体和血淋巴中也可以检测到。

The level of tumor necrosis factore alpha, interleukin-6 (IL-6) and interleukin-8 (IL-8) were evaluated using ELISA method. Western blotting was used to detect the protein expression of protein kinase B and lipid phosphatase PTEN. Immunoprecipitations and radioactivity of γ〓P incorporated into its specific substrate histon H〓B was utilized to determine the PKB activation. Phosphate concentration released from PTEN-specific substrat diC16PIP3 was assessed using green reagent method.

以细胞计数、噻唑蓝比色法测定细胞增殖能力，酶联免疫法检测培养上清液中细胞因子TNFα、IL-6、IL-8水平，Western免疫印记定量蛋白激酶B、蛋白磷酸酶PTEN表达水平，免疫沉淀、特异底物组蛋白H2Bγ〓P掺入量测定PKB活性，特异底物dic16PIP3脱磷酸绿色试剂法反应PTEN活性。

At embryonic day 14, MEFs were recovered from the embryos and cultured under the selection of HAT (0.016 mg of hypoxanthine/ml, 0.01 mmol/L aminopterin, 0.0048 mg of thymidine thymidine/ml), and the cell cycle, apoptosis, and the relative protein expressions were analyzed by flow cytometry and Western blot.

用不同剂量的电离辐射或紫外光分别照射ES或EF细胞，然后利用流式细胞仪检测该突变对小鼠ES细胞周期及对ES和EF细胞凋亡的影响，并利用Western blot检测相关蛋白的表达。

The expression and immunogenicity of fusion protein were detected by SDS-PAGE and Western blot analysis.

应用SDS-PAGE和Western blotting 技术分离并检测融合蛋白在宿主菌中的表达活性。

SDS-PAGE and Western Blot analyses showed that target protein was expressed mainly in the form of inclusion body.

经SDS-PAGE和Western Blot分析，四价抗体主要以不溶性的包涵体的形式表达。

SDS-PAGE and Western Blot analysis showed that scFv was expressed mainly as inclusion body in the cytoplasm of E.

经SDS-PAGE和Western Blot分析，单链抗体主要以包涵体的形式在大肠杆菌中表达。

MPGES-2 immunostaining remained a strong level from day 1 morning to day 2 evening, slightly increased from days 3 to 4, and dropped to a low level from days 5 and 6. During early pregnancy, mPGES-2 expression pattern shown by Western blot was similar to that of infundibulum by immunohistochemistry.

利用Western blot检测mPGES-2蛋白在早期妊娠输卵管中表达的结果与免疫组织化学检测伞部的结果基本一致。mPGES-2 蛋白在壶腹部的免疫染色整体稍弱于伞部。

Recombinant bovine prion protein was treated by chemical solution, and the treated Recombinant bovine prion protein was intracerebrally inoculated in the brain. The prion gene expression was quantified and the proteinase resistant protein was detected by western-blotting.

对重组牛朊蛋白进行了化学处理后脑内接种金黄地鼠，200天后对朊蛋白抗性进行了western-blotting检测，对脑组织朊蛋白基因的表达进行了分析。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。