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Methods Patients enrolled were divided into different groups randomly. Different methods were used to rinse the reusing dialyzers to remove germicide. Renalin residual test strips were used to examine the renalin residual density. Results were rewrdedand clinical symptoms were observed.
选择广东省佛山市第二人民医院血液净化中心长期血液透析患者47例随机分组,应用不同方法对透析器进行杀菌剂清洗,应用Renalin残余测试条检测透析器内残余的Renalin浓度,记录结果,观察患者临床表现。
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Methods One hundred and twentythree patients with severe pneumonia were divied into two groups,the second level antibiotics were used in control group untill children cured;sequential antibiotic therapy was adopted in observation group:the second level antibiotics were used first of all,after the complications were controled for 1~3 days,the first level antibiotics were used until children cured.
123例重症肺炎患儿分为2组,对照组在第2级选用抗菌谱宽、耐酶的抗生素,应用到患儿痊愈;观察组采用抗生素序贯疗法,首先在第2级选择抗生素,在重症肺炎的并发症控制后1~3 d,再在第1级中选择抗菌谱窄或不耐酶抗生素,直至痊愈。
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Methods Cells were maintained in DMEM medium with 10% fetal bovine serum. Five days before the beginning of experiments, the cells were seeded in phenol red-free DMEM medium containing 5% charcoal dextran-treated FBS. The cells were harvested, and seeded in 6-well culture plates or in 75ml flacks. After NP, BisA and DBP treatments for 72h, the cells were harvested, and mRNA and protein expression of PCNA, bcl-2 and bax were detected by reverse transcription-polymerase chain reaction and immunohistochemistry, respectively.
方法]T47D细胞在含10%胎牛血清的DMEM培养液中进行常规传代培养,实验前将细胞转移至无酚红DMEM(含5%活性碳葡聚糖苷处理过的FBS)中继续培养5d,收集细胞,PBS洗涤后接种於内置盖玻片的6孔板或75ml培养瓶中,用32×10^(-7)mol/L NP、32×10^(-7)mol/L BisA及32×10^(-6)mol/L DBP对细胞分别处理72h,用半定量RT-PCR技术观察这3种化合物对核增殖抗原PCNA、bcl-2及bax mRNA表达的影响,并用免疫组化方法对结果进行验证。
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Methods: Seventy five cases of juvenile endemic goitres were randomly divided into two groups. One was treated with combined auricular acupuncture and iodine salt, the other were fed only iodine salt.There were some auricular points selected, such as jing, neifenmi, pizhixia, pi, wei, gan, shen. Both of them were treated for three periods of treatment. The clinical results of the two groups were observed.
选择居住在碘缺乏地区 5 年以上、年龄12~17岁、经触诊法证实为甲状腺肿大Ⅰ度以上的患者75例为观察对象。75例随机分为耳针配合加碘盐治疗组和单纯食用加碘盐对照组,治疗组给予耳针治疗,采用毫针针刺,7 d 为一疗程,共治疗3个疗程,比较2组治疗前后尿碘的变化及治疗效果。
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Results: The peak responses of postprandial glucose and C-peptide were postponed; their values and the incremental area under curve of glucose responses of gruels were obviously lower than that of steamed bun (P.05). While both glycemic index and C-peptide index of steamed bun were set as 100, the glycemic index and C-peptide index of gruel A were 67, 99, and of gruel B were 57, 97 in NIDDM patients, 53, 69 in healthy subject.
结果 1。三组餐后血糖、C肽峰值显著低于馒头,血糖反应增值面积有显著性差异,以C组最低,A组最高;2.A、B两组C肽曲线增值面积显著低于C组;3.A、B、C三组血糖、C肽指数分别为67、99,57、97和53、69;4.B、C组血糖指数显著低于A组,C组的C肽指数明显低于A、B组。
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The results showed that 9 pathogen collections of Sporisorium reilianum were divided into 3 pathogenic types in which there were type A with high pathogenicity, type B with moderate pathogenicity and C with weak pathogenicity. Type A were originated in most important corn-grown areas including Changchun ,Jilin,Gongzuling and Changling where corn head smut usually occurs severely, type B were collected from Siping,Tonghua ,Songyuan,Yanbian, and type C were collected from Baicheng where corn head smut usually occurs very lightly.
致病类型A为强致病类型,来源于吉林、长春、公主岭和长岭,是玉米丝黑穗病发生最严重的地区;致病类型C为弱致病类型,分布于白城地区;致病类型B其致病性介于A、C类之间,主要分布于四平、通化、松原和延边地区。
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Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.
并以纯化的M蛋白为抗原,免疫BALB/c小鼠,按常规方法进行融合,间接ELISA方法进行筛选,采用有限稀释法,经过三次克隆,最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5),分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类,三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。
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Methods Fifteen healthy, female SD rats were employed in the experiment. Sensitized systematically with keyhole limpet hemocyanin, the animals were inoculated with the same antigen through cochlea basal turn into the labyrinth. Adminstrated 5-bromo-2′-deoxyuridine intraperitoneally, the rats were sacrificed and the temporal bones were harvested at 3, 7,14 day after labyrinth vaccination respectively. The frozen sections of the decalcified samples were dealt with H-E staining and immunohistohemical methods to investigate the cellular infiltration, BrdUrd and IgG positive cells in the ES.
选用SD大鼠15只,以钥孔虫戚血蓝蛋白(keyhole limpet hemocyanin,KLH)全身免疫后,经耳蜗底回钻孔以相同抗原进行内耳免疫,然后分别在内耳免疫后3、7和14 d腹腔注射溴脱氧尿嘧啶核苷(bromodeoxyuridine,BrdUrd)后处死动物,取颞骨经组织学处理制作冰冻切片,用免疫组织化学技术观察内淋巴囊的细胞浸润、增殖和IgG细胞的分布状况。
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Chemical and microbiological compositions of 12 samples of traditionally home-made koumiss in Xingjiang Uighur Autonomous Region were determined. The average contents of fat, protein, lactose, ash, calcium and phosphorus were 2.07g/100mL, 2.22g/100mL, 3.03g/100mL, 0.28g/100mL, 82.27mg/100mL and 58.20mg/100mL respectively. On average, pH was 3.90, titratableacidity was 134.25°T, lactic acid content was 10.99g/L, acetic acid content was 1.50g/L, citric acid content was 0.09g/L, ethanol content was 14.94g/L. The counts of lactic bacteria in 10 samples were 1.07~9.70×107cfu/mL, 1 sample was 1.38×108cfu/mL, another sample was 2.06×106cfu/mL; the counts of yeast in 1 samples was 3.20×104cfu/mL, 5 samples were 1.80~6.60×105cfu/mL, and 6 samples were 1.06~2.72×106cfu/mL. 33 strains isolated from 12 samples of traditionally home-made koumiss collected from Xingjiang Uighur Autonomous region belonged to Lactobacillus.
本项研究,对采集于新疆伊犁哈萨克族自治州、博尔塔拉蒙古族自治州和巴音郭愣蒙古族自治州 3 个地区的 12 份酸马奶样品,进行了化学和微生物组成分析,并对从中分离到的 33 株杆菌分离株,进行了属种鉴定,同时还对耐酸性菌株进行了初步筛选,结果表明: 12 份酸马奶样品的脂肪平均含量为 2.07g/100mL ,蛋白质平均含量为2.22g/100mL,乳糖平均含量为 3.03g/100mL,灰分平均含量为 0.28g/100mL,钙平均含量为 82.27mg/100mL,磷平均含量为 58.20mg/100mL;pH 值平均为 3.90,酸度平均为 134.25oT,乳酸平均含量为 10.99g/L,乙酸平均含量为 1.50g/L,4 份未检出,8 份柠檬酸平均含量为 0.09g/L,乙醇平均含量为 14.94g/l;1 份乳酸菌数为1.38×108cfu/mL,1 份为 2.06×106cfu/mL,其余 8 份在 1.07~9.70×107cfu/mL 间;1份酵母菌数为 3.20×104cfu/mL,5 份在 1.80~6.60×105cfu/mL 间,6 份在 1.06~2.72×106 cfu/mL 间。
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The probability grading for quantitative characters 400 large-flowered cultivars were chosed and their eighteen chief quantitative characters were tested and recorded according to the test method of morphological characters in the .The empirical distributions were determined by nonparametric test after the distribution range,mean value and variation coefficients of these characters were calculated and frequency diagrams were drawn.
数量性状分级选择代表性强的400个大菊品种,参照《菊花DUS测试指南》的形态性状测试方法,检测其18个主要的数量性状,并对性状的分布范围、平均值、变异系数进行统计计算,在此基础上,绘制了分布频次图,结合非参数检验判断性状的分布类型。
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- 推荐网络例句
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"Yes, now you can give yourself airs," she said, you have got what you wanted.
"对了,您现在高兴了,"她说道,这是您所期待的。
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Then the LORD said to me: Rebel Israel is inwardly more just than traitorous Judah.
上主于是对我说:"失节的以色列比失信的犹大,更显得正义。
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I do my best to guide my congregation,ok?
我尽了全力引导会众明白吗?