查询词典 were it not for

Methods Fourteen mutated vpr fragments were selected from patients with HIV. Both eukaryotic expression vector pcDNA3.1 and PCR products were purified, double-cut by Hind Ⅲ and BamH Ⅰ, and the cut products were legated and transformed into competent cells JM109. The 14 reconstructed plasmids were transfected into Hela cells. Cells with pcDNA vpr-wt, pcDNA vpr-Fs and pcDNA3.1 blank cells, and without pcDNA3.1 cell were established.

以14个带有HIV-1vpr基因片段的PcD-NA3.1真核表达载体构建重组质粒，将其转染Hela细胞，并设立保守株vpr基因转染细胞、突变株vpr-FS基因转染细胞、空载体转染细胞和未转染细胞作为对照，经逆转录多聚酶链式反应检测目的基因转染成功后，Pi染色，用流式细胞仪检测被转染细胞的细胞周期分布和细胞凋亡率。

METHODS: Umbilical cord blood samples were sterilely isolated using Percoll density gradient centrifugation to harvest intermediate layer cells. DMEM medium containing fetal bovine serum, penicillin, streptomycin and L-glutamine was added. Following several adherences and purification, the floating cells were discarded. Thus, many adherent cells with a confluence were collected. When cells were 60%-80% confluent, cells were digested by trypsin for subculture.

无菌条件下，应用Percoll密度梯度离心法分离脐血标本，收获中间层细胞，加入含胎牛血清、青霉素和链霉素、L-谷氨酰胺的DMEM基础培养液，再经反复贴壁纯化，除去悬浮生长细胞，得到较多呈融合状态的贴壁细胞，待细胞达60%~80%融合时胰酶消化传代。

RESULTS:Of the 82 ADR cases,51.22% were reported by doctors and 48.78% were reported by nurses;92.68% were intervenous drop infusion;53.66% were associated with antibacterials;And each 21.39% were manifested as lesions of skin and the appendants and general damage or loss.

结果：报告人以医生和护士居多，分别占总例数的51.22%和48.78%；静脉滴注方式导致的不良反应例数最多(92.68%)；导致不良反应的药品以抗微生物药物最多(53.66%)；临床表现以皮肤及其附件损害和全身性损害为主(各21.39%)。

BP, SpO2, EKG, PETCO2, cerrical contraction , fetal cardiac sounds were monitored continuously in the perinatal analgesia pe riod. The analgesia effects were determined by giving a mark for pain by VAPS, motor nerve blockings were given amark by Bromage scale; Apgar scale were conduc ted 1-5 min after neonates were labored out.

围分娩镇痛期连续监测血压、脉氧饱和度(SpO2)、EKG、PETCO2、宫缩及胎心音，记录产程时间；镇痛效果采用 VAPS 评分法进行疼痛评分，运动神经阻滞以 Bromage分级评分；新生儿出生后1～5 min进行 Apg ar评分，分娩后24 h新生儿 NACS评分。

The objective was to determine the effects of active immunization of T-3-BSA in Rom cockerels, 400 cockerels were divided into 4 groups, group 1,2 and 3 were treated with T-3-BSA on different dose(0.2,0.4,0.8mg/mI respectively),within each group,there were 5 treatments following different vaccination schedules, group 4 served as controls. Every injection of immunogen was conducted on different sites around the neck, and the datas were measured at week 11,15,19, combs,wattles,body weight,serum T concentration,carass traits and testicular development and histology were studied in order to find out the most reasonable vaccination schedule and the most possible mechanism in the H-P-G axis of T.

本试验测定了睾酮—3—牛血清白蛋白(T-3-BSA)主动免疫对公鸡性腺与冠垂发育及雄激素水平的影响。400只罗曼蛋用雏公鸡除随机取25只作对照组外，另375只随机分成3大组，每大组125只再随机分成5小组，每小组25只，3大组分别使用0.2mg/ml、0.4mg/ml和0.8mg/ml的T-3-BSA免疫注射，每大组的①②③小组分别于3、6、9周龄免疫注射，④小组于3周龄首次免疫，6周龄加强免疫，⑤小组于3周龄首次免疫，9周龄加强免疫。

Different surface treatments were taken on round gold surface, including no treatment, 50μm Al_2O_3 sand blasting, 110μm Al_2O_3 sand blasting, gold bonder applying after 50μm Al_2O_3 sand blasting, gold bonder applying after 110μm Al_2O_3 sand blasting, and the same nine Ni-Cr alloys were cast as contrast, the gold copings and Ni-Cr alloys were both received two layers of opaque and two layers of body porcelain. Shear tests were performed in a universal testing machine. After loading the sections were examined by SEM and EDS analysis.

第一部分金沉积基底不同表面处理对金瓷剪切强度的影响材料与方法：制作直径4.0 mm，高度为8.0 mm的圆柱形Ni-Cr合金试件45个，表面电镀形成金沉积帽状基底，随机平均分成5组，分别为表面未处理组、50μm、110μm Al_2O_3喷砂组、50μm Al_2O_3喷砂+金黏结剂组、110μm Al_2O_3喷砂+金黏结剂组；另铸造同样规格的Ni-Cr合金试件9个，作为对照。

Bacteria on burn wound and alive tissue under burn wound of the 119 patients that were treated with MEBT was generally cultured and identified, when these patients were first debrided ,7 days later and before the wound healing.30 patients randomly selected from 119 patients were enrolled in the study ,bacterial quantity and identification in burn wound tissue were carried out, bacterial epidemiology data in burn ward were analyzed in the same period.

对应用MEBT治疗的119例烧伤患者的创面于首次清创时、入院后一周、创面愈合之前作常规细菌学培养、鉴定，同时抽取其中30例进行烧伤创面坏死组织及其下活组织细菌定量计数及菌种鉴定，并与同期烧伤病房环境的细菌流行病学资料进行了相关分析。

MEBT.. Method Bacteria on burn wound and alive tissue under burn wound of the 119 patients that were treated with MEBT was generally cultured and identified, when these patients were first debrided ,7 days later and before the wound healing.30 patients? randomly selected from 119 patients were enrolled in the study ,bacterial quantity and identification in burn wound tissue were carried out, bacterial epidemiology data in burn ward were analyzed in the same period.

对应用MEBT治疗的119例烧伤患者的创面于首次清创时、入院后一周、创面愈合之前作常规细菌学培养、鉴定，同时抽取其中30例进行烧伤创面坏死组织及其下活组织细菌定量计数及菌种鉴定，并与同期烧伤病房环境的细菌流行病学资料进行了相关分析。

Methods: the chondrocytes of the rabbit condylar were derived from rabbit,and were cultured to the second generation in vitro,then hgfcdna transfected the cells.the capacity of cell proliferation was tested by mtt,the deferences of the type ii collagen were tested by sabc immunohistochemical of the type ii collagen after infected;the mrna levels were tested nd so on the mrna levels were tested by in situ hybridization of the type ii collagen between the transfection group and comparison group .

取兔膝关节软骨细胞体外培养至第二代，hgfcdna转染软骨细胞，空白质粒组为对照组；噻唑蓝检测软骨细胞的增殖能力，sabc免疫组化及原位杂交法测其ii型胶原在转染前后的变化与mrna的表达。

Methods: Forty-two maintenance hemodialysis patients were randomly divided into two groups (twenty-one patients each group): in treatment group high-flux dialysers were used, and in control group, low-flux dialysers were used. They were treated for 12 weeks. The clinical symptom, blood β2 microglobulin (β2-M), paratyyroid hormone, serum phosphate, albumin, serum creatinine, blood urea nitrogen were determined respectively before and after the treatment.

选择42例维持性血液透析患者，治疗组和对照组各21例，治疗组采用面积为1.8 m2的高通量透析器，对照组采用面积为1.3 m2的普通透析器，连续观察12周，分析患者症状改善情况，并测定首次透析前及12周后的血β2微球蛋白(β2-M)、甲状旁腺素、血磷、清蛋白、血肌酐、血尿素氮等指标。

"Yes, now you can give yourself airs," she said, you have got what you wanted.

"对了，您现在高兴了，"她说道，这是您所期待的。

Then the LORD said to me: Rebel Israel is inwardly more just than traitorous Judah.

上主于是对我说：＂失节的以色列比失信的犹大，更显得正义。