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After the cells were treated by high concentration toxin for 4d, the content of nitrate-nitrogen in cells were lower markedly than the contrast, after the cells were treated by middle and low concentration toxin for 7d, the content of nitrate-nitrogen in cells were lowered. 3 groups of toxin treating all lowered the total phosphorus content. At the end of test, the phosphorus contently of low, middle, high concentration treating groups were 74.98%, 76.47% and 84.00% of the contrast respectively. The ACP activity of 3 treating groups compared with the contrast appeared the tendency of first lowering then raising.
高浓度毒素处理细胞4d后,细胞内硝态氮含量显著低于对照;中、低浓度毒素处理细胞7d后降低了胞内硝态氮含量。3组毒素处理均降低了胞内总磷含量,到实验结束时,低、中、高浓度处理组的胞内磷含量分别为对照的74.98%、76.47%和84.00%。3组处理组ACP活力与对照相比呈现先降低后升高的趋势。
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The equivalent standard pollution loadings were compared among different rigions, the results indicated that the water pollution threaten caused by agricultural non-point source pollution was more serious in the south of Jiangsu province and in Nantong than in the other rigions;The equivalentStandard pollution loading rates of different agricultural non-point source pollution in different regions were analysised by clustering analysis method,and the results indicated that Nanjing、 Wuxi、 Zhenjiang and Changzhou were polluted mainly by domestic living pollution;Xuzhou、 Suqian、 Taizhou、 Yancheng、 Lianyungang、 Huaian and Nantong were polluted mainly by chemical fertilizer pollution; Suzhou and Yangzhou were polluted corresponsively by animals feces pollution 、 domestic living pollution、 chemical fertilizer pollution and fish breeding pond pollution.
对各地区的等标污染指数进行比较,发现农业非点源污染对水环境造成的威胁苏南地区和南通明显高于其它苏中、苏北地区;对各个市各非点源污染源的污染负荷率进行聚类分析,发现南京、无锡、镇江、常州以生活污染为主;徐州、宿迁、泰州、盐城、连云港、淮安、南通以化肥污染为主;苏州、扬州两地畜禽粪便污染、生活污染、化肥污染和精养鱼塘污染均占一定的比例。
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Methods There were 100 cases in normal group and 36 cases in group of chest injuries. All cases were performed in volume scan according to conventional chest scan by SIEMENS Sensation 4 MSCT , then performed in thin slice low and high contrast image reconstructions. After that, all the source images were input into CT 3D work stations,costicartilage were imaged by post process software such as multiplanar reconstructions, maximum intensity projection, surface shade display and volume rendering technique. All the pictures were observed and analyzed by two radiologists.
利用西门子Sensation 4 多层螺旋CT机按照胸部常规扫描条件对胸部外伤组36例和对照组100例患者进行容积扫描,然后进行薄层低对比及高对比图像重建,并将重建图像导入CT三维(3D)工作站,利用多平面重建成像、最大密度投影、表面遮盖法成像及容积成像技术对图像进行后处理,由2名CT诊断医生一起对各种后处理图像进行观察和分析。
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The correlations of eleven floral and fruit quantitative traits of Liriodendron interspecific hybrids(L. chinense×L. tulipifera) and its parent species were analysized.The aglycone and glycoside of flavonoid and carotinoids of perianth segment in the three groups were studied qualitatively and quantatively by high performance liquid chromatography.The results were as followed: The aglycone such as cyanidin, 6-hydroxyflavone and trans-chalcone and the glycoside such as glucose, rhamnose and galactose were discovered in Liriodendron interspecific hybrids and Liriodendron chinense,carotinoids such as lycopene and β-carotene were also discovered.
本文对鹅掌楸、北美鹅掌楸和杂交F_1的花果共11 个数量性状进行分析,并应用高效液相色谱对上述三大类群花被片类黄酮糖苷配基和结合糖及类胡萝卜素进行了定性和定量研究,结果如下:鹅掌楸和杂交鹅掌楸花被片类黄酮糖苷配基为花青素、6-羟基黄酮和反式查尔酮,糖苷配基结合糖为葡萄糖、鼠李糖和半乳糖;类胡萝卜素为β-胡萝卜素和番茄红素。
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objective: to investigate diagnosis and treatment choice of detrusor hypoactivity with impaired contractile function on voiding function in benign prostatic hyperplasia.methods:the clinical date of 48 bph patients with dhic were analyzed,including clinical diagnosis,urodynamic parameters,treatment and follow-ups.results:37 patients with detrusor instability and over 40 cmh2o bladder pressure were treated successfully by turp.the time of indwelling suprapubic punctural cystostomy cathter was(1~8)weeks.11cases without di and under 40 cmh2o bladder pressure were treated by suprapubic punctural cystostomy,accepted the test of urodynamics after(1~3)months.7 cases with di were treated successfully by turp,4 cases without di were indwelled suprapubic punctural cystostomy cathter all life.conclusion:dhic worsens the voiding function of the patients with bph,and the test of urodynamic is helpful to diagnose and could provide more convinced evidences for treatment of patients.
分析膀胱过度充盈所致逼尿肌损伤,引起膀胱逼尿肌收缩无力良性前列腺增生48例患者的临床资料,包括诊断,尿动力学检查,治疗及随访结果。结果:37例经尿流动力学检查,诱发存在逼尿肌不稳定,伴膀胱内压达40 cmh2o以上,采取turp,术后留置膀胱造瘘管(1~8)周后全部拔除,排尿通畅。11膀胱内压始终未达到40 cmh2o,且在膀胱注水充盈过程中无逼尿肌不稳定出现,行膀胱造瘘后出院,(1~3)个月后经尿动力学检查复查膀胱逼尿肌收缩力,其中7例膀胱逼尿肌收缩功能有恢复,行经尿道前列腺汽化电切术治愈;剩余4例膀胱逼尿肌收缩功能几乎无任何改善。长期留置膀胱造瘘治疗。结论:良性前列腺增生导致膀胱过度充盈所致逼尿肌损伤,引起膀胱逼尿肌收缩无力患者,术前应用尿流动力学检查对膀胱逼尿肌损伤程度进行认真分析,可进行有针对性的治疗。
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Results 26 cases,the intraspinal lipoma were five cases,three cases were teratoma,5 cases were the separation of fetal spinal cord,three cases were superficial sinus,4 cases were diastematomyelia.
结果 26例中,椎管内脂肪瘤5例,畸胎瘤3例,脊髓胎分出5例,皮毛窦3例,脊髓纵裂4例,隐性脊柱裂6例。
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Three aspects were included here:The derivatives of dihydrol,2,4,5-tetrazine-3,5-dicarboxylic acid were synthesized via three different routes:(1) four new compounds were synthesized via the reaction bet ween dihydrol,2,4,5-tetrazine-3,5-dicarboxylic acid, thionyl chloride and corresponding alcohol ;(2) first ,dimethyl dihydro-1,2,4,5-tetrazine-3,5-dicarboxylate wer e synthesized, then ,another four new compounds were obtained t hrough the alcoholysis under the catalyst ;(3) three acylamines were got through the ammonolysis betw een dimethyl dihydro-1,2,4,5-tetrazine-3,5-dicarboxylate and amines.
本论文分别通过三条不同的路线合成了1,4-二氢-3,6-二甲酸-s-四嗪的衍生物:(1)通过1,4-二氢-3,6-二甲酸-s-四嗪与氯化亚砜和相应的醇反应,制备了四个新化合物;(2)合成1,4-二氢-3,6-二甲酸甲酯-s-四嗪,在催化剂的作用下,与高沸点的醇进行酯交换反应,合成了四个新化合物;(3)合成1,4-二氢-3,6-二甲酸甲酯-s-四嗪后,与胺进行氨解反应,合成三个新的1,4-二氢-3,6-二甲酰胺-s-四嗪化合物。
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Methods The NGF, BDNF, and NT3 genes of rats were cloned, the eukaryote expression vectors were established, the three kind of recombinant vectors were used to transfect astrocytes, the positive cloned cells were cultured dilatedly after G418 sifting; using supernates of culture liquid of astrocytes modified by gene to culture PC12 or TrkB-PC12, the expression and its level of gene target cells aimed genes were measured by Western blotting or immunohistochemical method.
克隆大鼠NGF、BDNF和NT3基因,构建真核表达载体;3种重组载体分别转染星形胶质细胞,G418筛选后获得阳性克隆细胞进行扩大培养;取基因修饰星形胶质细胞培养液上清培养PC12细胞或TrkB-PC12细胞;用Western blotting杂交或免疫组织化学方法检测基因靶细胞目的基因的表达及其水平。
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CuS and CdS nano-particulate films were obtained with these two methods, and some interesting phenomena were observed: under the Gibbs film formed by CTAB, CuS nano-rods were obtained at the oil/water interface, and disklike aggregates of CdS nano-crystals were obtained at both the air/water and oil/water interfaces; under the Langmuir film formed by NaAOT at air/water interface, disk-like aggregates of CdS were also obtained.
利用此两种反应技术,制备了CuS和CdS颗粒膜,并研究了它们的一些光电性质;在Gibbs膜下,得到了棒状CuS纳米晶。由发现的表面活性剂形成的带状物,提出棒状CuS晶体是以带状聚集体为模板形成的;在液-液、气-液界面Gibbs膜下反应中,发现CdS纳米颗粒的盘状聚集体的形成,这是由于表面活性剂在界面上形成的二维胶束而造成的;在气-液界面上Langmuir下的反应中,也发现CdS盘状晶体的生成。
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To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.
为研究磷酸化应激诱导蛋白的表达、定位和功能,采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段,经产物纯化、与载体连接,分别构建了STIP1基因的原核表达载体和真核表达载体;IPTG诱导原核表达载体,变性聚丙酰胺凝胶电泳与染色后,可见相应大小的蛋白质表达;STIP1基因的真核表达载体转染细胞系后,共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内;用药物筛选STIP1基因转染的细胞后,流式细胞仪检测细胞周期,可见G〓-G〓期和G〓-M期的细胞明显降低,S期细胞明显增多。
- 相关中文对照歌词
- Hela Världen För Mig
- Din Första Kyss
- Sov Gott
- Fernando
- En Livstid I Krig
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- Gott Mit Uns
- Ett Slag Färgat Rött
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- Baksmälla
- 推荐网络例句
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Liapunov—Schmidt method is one of the most important method in the bifurcation theory.
Liapunov—Schmidt方法是分叉理论的最重要方法之一。
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Be courteous -- even when people are most discourteous to you .
要有礼貌──即使当別人对你最不礼貌的时候。
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I think we have to be very careful in answering these questions, because nothing is really so simple.
我认为,我们在回答这些问题的时候应该非常谨慎,因为事情远没有那么简单。