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Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

The synthesis of the pentacyclic skeleton of Et-743 and the construction of the five chiral centers thereof.1. The core pentacyclic skeleton of phthalascidin was constructed with L-dopa as the starting material via thirteen steps. First, two basic synthons, the C-1 functionalized tetrahydroisoquinoline compounds 8 and N-protected Z-dopa derivative 9, were prepared from L-dopa. They were coupled via amide bond to form the dipeptide compounds 10, which were the first key intermediates. Then the primary hydroxyl groups were transformed into the corresponding amino aldehydes 11 via Swern oxidation. Followed by the intramolecular Pictet-Spengler cyclization, the pentacyclic compounds 12 were synthesized.

五环骨架的构建及五个正确手性中心的确立：1.1我们以L-多巴为起始原料，经过多步官能团保护和转化合成了基本结构单元8然后和苯丙氨酸衍生物9，通过缩合反应生成酰胺中间体10，经Swern氧化得到醛化合物11，然后再次利用分子内改良的Pictet-Spengler反应一步构建c，d两环，得到五环骨架化合物12，最后，实现了21-位羰基向氰基的转化，得到了C-1位官能化的化合物14，化合物14不但具有和Et-743一致的五环骨架，而且分子中五个手性中心立体构型也与其完全相同：其中，3，位和13，位的手性中心由原料L-多巴带入，而1，位，11，位和21，位手性中心则通过不对称诱导而确立。

In the low B condition of the soilless culture, rapeseed seedlings were short of B, the seedlings were short and the leaves were malformed and the weight of the shoots decreased, the branch roots were fewer and snuff color, the weight of roots decreased, and the length of root and the ratio of shoot/root decreased, too. However, the high B- efficiency type of rapeseed had the a great ability to the low B stress, the growth of shoots and roots were better than the low efficiency, and the B concentration range of the rapeseed varieties which responded to the B scarce was 0.001 mg/L to 0.01 mg/L.

营养液培养条件下，苗期缺硼，甘蓝型油菜植株瘦小，叶片皱缩，地上部生长量下降；侧根少且呈现黄褐色，根重、根体积、根长以及根冠比下降；高效品种具有较强的耐低硼胁迫的能力，表现为同等缺硼条件下，对高效品种地上部和根系生长的抑制作用都相对较小，且品种间对缺硼反应差异最大的硼浓度范围为0.001mg／L—0.01mg／L。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

Results: There 15 cases were pyretic (15.6%), 41 cases were masculine of peritoneum stimulate syndrome (42.7%), 64 cases were abdominal distention (66.7%), 18 cases were abdominal pain (18.8%), 47 cases were masculine of motion sonant (48.9%).

结果：发热15例(15.6%)，腹膜刺激征阳性41例(42.7%)，腹胀64例(66.7%)，腹痛18例(18.8%)，移动性浊音阳性47例(48.9%)。

All aforementioned cases entierely were proved pathological diagnosis using operation or lung centesis. Results: On the CT manifestation, lobulation sign and spiculation sign mostly appeared in the malignant nodules. The accurate rate of diagnosis was 75.6; sensitivity were 76.3%; specificity were 74.1%; positive predictive value were 86.5%; negative predictive value were 58.8%.

结果：86例SPN的Cr征象中，分叶征和棘突或毛刺征多出现于恶性结节，与病理和随访结果对比，螺旋CT薄层扫描并MPVR重建判断肺小结节良恶性的准确率、灵敏度、特异性、阳性预测值和阴性预测值分别为75.6%、76.3%、74.1%、86.5%、58.8%。

In Xinxiang Aera of Henan Province in 1997, the occurrence of cotton pests were investigated in the Bt cotton and common cotton fields The result showed that the numbers of cotton bollworm egg were similarly in both fields, but that of survival larvae on Bt cotton were lower than that on common cotton The occurrence of cotton aphis was no difference but the spider mite were more heavy in Bt cotton field At the same time,control strategy against cotton pests were discussed for the Bt cotton

对河南新乡棉区种植的转Bt基因棉和常规棉棉田虫情进行了定点系统调查和大田普查，结果表明：棉铃虫在两种棉田棉花上的落卵量无明显差别，而幼虫存量差异显著，Bt棉棉花上幼虫存量显著降低；Bt棉对棉蚜的发生无明显的影响，而Bt棉上棉红蜘蛛的发生比常规棉略重，同时初步研究制定出种植Bt棉后的棉虫综防对策

From April to September, several clusters of bamboos, 3 ~ 4 m long, 1 ~ 2 m wide, were set on the sea bed at a depth of 16 to 20 m to attract their spawning, and the egg-strings were then transported to the laboratory. When the development of the embryo reached stage 24, iris of eyes being prominent as a colour circle and statolith being formed, they were transferred into different rearing conditions, i.e., 20, 25, 30, 35 o/oo and 15, 20, 25, 30 oC. The durations from stage 24 to hatching were different among all different rearing conditions. The statoliths were extracted and mounted in Crystal Bond thermoplastic cement for reading their growth rings.

每年的四月到九月为东北角软丝主要的产卵季节，竹丛被设置在沙质海床上吸引成熟的个体前来产卵，将采集到的卵串带回实验室中培养，当胚胎发育至第二十四期的时候(胚胎的虹膜及平衡石开始生成)，这些胚胎分别被转换至不同的温度及盐度条件之下培养，温度被控制在15， 20， 25， 30 oC 四种条件下，盐度则分为20， 25， 30， 35 o/oo 四种条件，共组成十六种不同的培养条件，从平衡石生成至幼体孵出所需要的时间随著培养条件的不同而有所差异，孵化所需要的时间会随著培养的温度而有很大的变化，低温的条件会将所需的时间增长许多，在正常的条件下(25oC，35o/oo)，从平衡石出限制肤出约需要9~16天。

METHODS: CNE-2 human nasopharyngeal carcinoma cells were exposed repeatedly to γ-rays and then screened for a new radioresistant subline named CNE-2R. Double times of CNE-2 and CNE-2R cells were determined by MTT assays. The relative radiosensitivities of tumor cells were assessed by standard colony formation assays. Survival rates after exposed to irradiation were measured by MTT assays. The time courses of cell cycle distribution before and after irradiation were investigated by flow cytometry.

用γ射线反复照射CNE-2细胞，筛选出放射抗拒细胞株CNE-2R；检测细胞生长的倍增时间；应用细胞克隆形成实验检测细胞的放射敏感性；MTT法检测照射后不同时间的存活分数；用流式细胞术检测细胞的周期分布特征。

Moreover, the hot compressive deformation behavior of the RS/PM AZ91 magnesium alloy and AZ91 alloy matrix composite were also investigated, the conclusions are drawn as follows:1. The processing parameters of the atomization-twin rolls quenching technology were optimized as follows: the diameter of the nozzle 1mm, the wheel velocity 25m/s, the pressure of atomization gas 0.3MPa and Ar gas for melt injection 0.1MPa. The RS AZ91 alloy flakes exhibited fine and uniform microstructures. When the flakes were extruded at 673K, extruded velocity of 0.1mm/min and extrusion ratio of 25:1,the magnesium alloy rods with clean surface, uniform dimension and excellent mechanical properties were obtained.2. The RS AZ91 magnesium alloy powders prepared by atomization-twin rolls quenched technology exhibited fine equiaxed grains with the grain size of 1-3μm, the phase constituent included supersaturate solid solution phaseα-Mg and miner fineβ-Mg_(17)Al_(12) phase. The as-extruded materials also exhibited equiaxed grains with the size of 5-7μm and a large number of fineβ-Al_(12)Mg_(17) and fewer AlMg_2Zn phases were detected in the alloy.

本文还研究了快速凝固/粉末冶金AZ91镁合金热压缩变形流变应力行为，快速凝固/粉末冶金法原位生成Mg_2Si增强AZ91镁基复合材料，经过系统的研究，获得如下结论：1、雾化-双辊急冷法在下列工艺参数组合下：双辊线速度25m/s；喷嘴直径1mm；熔体压射压力0.1MPa；雾化气体压力0.3MPa。，可获得宏观尺寸细小、微观组织均匀细小、综合性能优良的的快速凝固AZ91镁合金细碎箔带，快速凝固箔带在挤压温度为673K、挤压速度为0.1mm/min，挤压比为25：1时，可获得的外表光洁、尺寸均匀、组织性能优异的镁合金棒材。2、雾化-双辊急冷法制备的AZ91镁合金粉末态为细小等轴晶组织，晶粒尺寸1-3μm，组织为α-Mg过饱和固溶体和微量的细小β-Mg_(17)Al_(12)相组成；粉末挤压棒材为等轴晶组织，晶粒尺寸5-7μm，组织中含有大量细小的β-Al_(12)Mg_(17)以及AlMg_2Zn析出相，室温力学性能，抗拉强度383MPa，屈服强度275MPa，断后伸长率7.5%。

Putt your way through 36 fun-filled holes of minigolf on 3D designed courses with elevated greens, bunkers, bridges and water hazards, among other crazy obstacles.

您的推杆方式，通过36个有趣的填孔迷你的三维设计的课程，以提升绿党，掩体，桥梁和水的危害，除其他疯狂的障碍。

Some participles can be used either as attributes or as predicatives.

有些分词既可当定语用，也可当表语用。