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There were very significant differences about the values of MP, MPF and AEMG among the motion muscles, but there was no significant difference about the singals of MF slope, MPF slope and AEMG slope. In patients group there were significant differences about the values of MF, AEMG, AEMG slope of motion limb between paretic side and non-paretic side. There were also significant differences about the values of MPF slope between paretic side of the patients and left side of the health. But there was no significant difference about other parameters in groups or among groups. Compared the activity muscles of four groups, there were significant difference about the values of AEMG and MPF among initiative muscles, antagonistic muscles and co-contraction muscles. There were very significant differences about the values of MF and MPF among co-contraction muscles, but there was no significant difference between initiative muscle and antagonistic muscle.

结果:两组各活动肢体间比较除AEMG斜率均值外MF、MPFAEMG及MF斜率及MPF斜率均值差异无显著性意义;各活动肌肉间比较MF、MPF、AEMG均值差异有显著性意义,而MF斜率、MPF斜率及AEMG斜率均值无显著性差异;活动肢体因素四个水平间比较除偏瘫组患侧与健侧之间的MF和AEMG、AEMG斜率均值和偏瘫组患侧与正常组左侧的MPF斜率均值有显著性差异外,其余参数在偏瘫组与正常组组内、组间的差异无显著性差异;活动肌肉因素4组肌肉间对比:主动肌与拮抗肌以及协同肌AEMG、MPF差异有显著性,协同肌之间MF、MPF差异有非常显著性,拮抗肌与协同肌差异无显著性。

Were extracted by different extracting solvents in lab, the results indicated that with the increasing of Mn concentration levels, the speciation of Mn in their roots transform from H2O-extractable species to HCl-extractable species and NaCl-extractable species, showed increases of Mn of oxalate binding and pectate binding or prontein binding in cell wall of roots; Mn of H2O-extractable species were 34.55~50.26% of total Mn in stems and increased with the increasing of Mn concentration levels, which benefited upward transport of Mn; Percentage of Mn of H2O-extractable species were 38.55~44.19% and Mn of HCl-extractable species were 20.66~28.74% to total Mn in leaves, suggesting that most Mn in leaves were the speciation of H2O-solubility organic salts or manganese oxalates. 5. Chlorophyll in leaves of Polygonum hydropiper L.

不同的提取剂对水蓼根、茎、叶的提取结果表明,随着锰处理浓度增加,水蓼根中Mn由水提取态向盐酸提取态和氯化钠提取态转化,说明根细胞壁结合的草酸盐和果胶酸盐或蛋白质结合态的Mn含量随锰处理浓度的增加而增加;在茎中,水提取态锰含量占总锰的34.55-50.26%,且随锰处理的增加其水提取态锰的比例增加,这有利于锰向上运输;水蓼的叶片中,水提取态锰占总锰的38.55-44.19%,盐酸提取态锰占总锰的20.66-28.74%,说明叶片中的锰大部分以水溶性有机盐或草酸锰的形态存在。

Many respondents were dissatisfied with every part of their own appearance - 87 per cent hated their " podgy thighs ", 79 per cent were unhappy with their waist , 65 per cent were disappointed with the size and shape of their breasts , the same number were unhappy with their feet 65 per cent , and 59 per cent were unhappy with their face .

87的人讨厌自己"短粗的大腿" 79的人对腰围很不满65的人对自己的胸部尺寸和形状感到失望同样65人不满意她们的脚59的人对自己的容貌不满意超过一半的人对她们的手和手指感到失望。

In the normal rats (n=5、6), CGRP-LI were mainly present in small and some medium-sized neurons and the primary afferent fibers and ter- minals in laminae Ⅰ,Ⅱ,Ⅴ,Ⅹ and Lissauer's tract of the spinal cord; GAL- and SP-LI were mainly present in small neurons and the superficial layers of the dorsal horn. After the adjuvant injection both CGRP-and SP-LI were in- creased corresponding to the development of arthritis. On day 2, CGRP-and SP-LI were moderately increased in laminae Ⅰ,Ⅱ and the posterior commis- sure of the spinal cord and small neurons in DRG, while SP was obviously in- creased in the superficial layers and DRG. On day 14, CGRP-LI were marked- ly enhanced in the above regions with the development of polyarthritis. Some- times many strongly stained long fibers could be seen in the posteriot commis- sure.

在DRG中,CGRP主要分布于小和中等神经元之中,SP和GAL主要分布于小神经元之中;2、注入弗氏完全佐剂(含冻干结核菌0.5mg)后第2天动物(各5~6例)产生具有明显红、肿、热、痛和痛敏的急性关节炎,免疫组化实验观察到注射侧脊髓背角和DRG中CGRP-和SP-LI明显增加;在佐剂后第14天,动物患肢肿胀、痛敏和自发痛均进一步加重,部分动物健肢也出现红肿,产生多发性关节炎时,脊髓背角浅层和DRG中CGRP-和SP-LI增加更为明显,并且在灰质后联合中可见许多深染的CGRP-LI长纤维;在佐剂后第21和28天,随着佐剂性关节炎的逐渐恢复,脊髓背角和DRG中CGRP-和SP-LI也接近正常。

Methods 56 patients in esophageal and gastric variceal bleeding were allocated randomly into the control group and the treatment group. All patients were given basic treatment, the CG patients received pituitrin (0.2U/min) though micro infusion pump,octreotide 0.1mg were injected in the TG patients and infused continuously at a rate of 25 礸/h. The efficient hemostasis, rebleeding, the average bleeding time and blood volume were recorded. Adverse reactions such as the queasiness, abdominal distension, abdominal pain, diarrhoea, palpitaition and hypertension were observed.

将经胃镜证实的食管胃底静脉曲张破裂出血患者56例随机分成对照组(n=28)和治疗组(n=28),两组在给予基础治疗的同时,对照组使用垂体后叶素0.2U/min微泵持续静脉滴注;治疗组使用奥曲肽,首次0.1mg静注,再以25μg/h微泵持续静脉滴注止血治疗,比较两组在止血有效率、再出血率、平均止血时间、平均输血量等止血效果及恶心、腹胀、腹痛、腹泻、心悸、胸闷、血压升高等不良反应上的差异,并进行统计学分析。

Different biological and ecological characteristics, virulence, sporulation, conidia bourgeon, Protea-Bse activity leve and colony growth of 6 strains of Beauveria bassiana were measured respectively. The results were calculated by grey relational analysis and the optimum sequence was built from the professional comparison of each strain's characteristics. Two strain's have a potential of appling in mass production, BD-B015 and BD-B038 were abtained. The revisory mortality to Laphygma exigua Hubner were 88.33% and 86.70%.The infected rate were 86.67% and 85.27%.

测定初筛获得的6个球孢白僵菌菌株的毒力、产孢量、萌发率、胞外蛋白酶活性及菌株生长速度等多个生物、生态学指标,从专业角度构建最优序列,经灰色分析和不同菌株毒力效价显著性比较,筛选出了两株具有较大生产潜力的优良菌株BD-B015和BD-B038,对甜菜夜蛾的校正死亡率分别为88.33%和86.70%,僵虫率分别为86.67%和85.27%。

Results: 1. Childhood trauma history: the patients with BPD had significantly higher scores of emotional abuse, physical abuse, sex abuse, emotional neglect and physical neglect than the non-personality disorder patients. 2. The parental rearing style: comparing with the Chinese norm, the scores of emotional warmth / comprehension of BPD group were lower; the scores of punishment/rigorousness and rejection /deny of BPD group were higher; The differences were significant. 3. Family environment: comparing with Chinese norm, the scores of intimacy/ affection expression/ independence/ success/ culture/ entertainment/ morality and religion/sense of organization of BPD group were lower; the scores of contradictory were higher.4. Correlation analysis revealed that the childhood trauma history of BPD was correlated with the unhealthy parents' rearing style and family environment. 5. Logistic regression analyses revealed that gender, emotional abuse, physical abuse, emotional neglect are the risk factors for BPD.

结果1、BPD儿童期创伤经历:BPD患者在情感虐待、躯体虐待、性虐待、情感忽视、躯体忽视及总体虐待程度显著高于精神科门诊中非人格障碍患者。2、 BPD父母养育方式:BPD患者的父母养育方式与中国常模相比存在较多显著差异,父亲在情感温暖理解、过度保护方面远低于对照,而在惩罚、严厉,拒绝、否认方面远高于对照;母亲在情感温暖、理解方面远低于对照,而在拒绝、否认,惩罚、严厉方面远高于对照。3、 BPD家庭环境:患者家庭的亲密度、情感表达、独立性、成功性、文化性、娱乐性、道德宗教观、组织性等方面均显著低于中国常模;而矛盾性方面显著高于常模。4、 BPD儿童期创伤与不恰当的父母养育方式和不良的家庭环境显著相关。5、BPD的影响因素:经Logistic回归分析,性别、年龄,情感虐待、躯体虐待、情感忽视是影响BPD患病与否的关键因素。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上,加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组;将SD仔鼠软骨细胞复合于支架上,常规培养2周作为对照组。20只裸小鼠,左侧腋窝皮下均植入实验组细胞-支架复合物,右侧腋窝皮下均植入对照组细胞-支架复合物。8, 12周各处死10只,行甲苯胺蓝和番红O-固绿染色。

In this research, the activities of CWDE were measured by saccharifying according to the theory that red-brown amino compounds were produced when 3, 5-dinitro salicylic acid and aldehyde were heated together. And the testing conditions were made clear. The activity of Cx should be tested with optimal temperature of 50℃, optimal pH of 4.8, incubation time of 30 min, and optimal substrate of 0.6% CMC-Na. Due to the heat-libality, Cx should be sterilized more than 10 min. The testing conditions of the activity ofβ-G were almost the same with Cx except the substrate of 0.5% salicin and the sterilization time of more than 15 min.

主要取得了一下成果:(1)明确了条锈菌细胞壁降解酶活性测定的条件:羧甲基纤维素酶活性的测定条件为50℃,pH4.8,反应时间30min,底物浓度为0.6%羧甲基纤维素钠,以DNS法测定,且失活酶液的灭活时间应大于10min;β-葡萄糖苷酶活性的测定条件为:50℃,pH4.8,反应时间30min,底物浓度为0.5%水杨苷,以DNS法测定,且失活酶液的灭活时间应大于15min;果胶酶活性的测定条件为:50℃,pH4.8,反应时间1h,底物浓度为0.25%果胶,以DNS法测定,且失活酶液的灭活时间应大于10min。

The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphic, and 4~9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cultivars was between 0.17~0.72, average value was 0.39. When all of the polymorphic bands were analysed by cluster analysis, 19 potato cultivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato cultivars is wide.

利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析,分别提取19份马铃薯品种的DNA,进行随机引物多态性扩增,从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究,每条RAPD引物扩增出4~9条带,共获得52条带,其中多态性条带为43条;19份马铃薯品种的遗传距离介于0.17~0.72之间,平均值为0.39,平均遗传距离介于0.31~0.51之间;聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类,聚类结果与系谱分析基本相符,同时也说明克新系列马铃薯的遗传基础有所拓宽。

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