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Results: a In the negative control group, spontaneous chromosome aberration and micronucleus rated the mice's were very low; in the positive control group, chromosome aberration and micronucleus rate of the mice's were highest. b Before cyclophosphamide intoxation, when the four kinds of vegetables and hilts juice were preventively irrigated into the mice's stomachs respectively, the chromosome aberration and micronucleus rate in the negative control group were lower than these in the positive control group (P.05); but after intoxation, when the four kinds of vegetables and fruits juice were therapeutically irrigated into the mice's stomachs respectively, the chromosome aberration and micronucleus rate were similar with the result above mentioned . c Acing the four kinds of vegetables and fruits, sea-buckthorn lad the best preventive and curative effects.
结果:a在阴性对照组中,小鼠自发的染色体畸变率和微核率极低;在阳性对照组中,小鼠染色体畸变率和微核率最高。b在环磷酰胺染毒之前,分别将4种蔬菜水果汁给小鼠进行预防性灌胃,其染色体畸变率和微核率均比阳性对照组低(P.05);在染毒之后,分别将4种蔬菜水果给小鼠治疗性灌胃,其结果与上述实验结果相似。c在4种蔬菜水果中,以沙棘的预防和治疗效果为最好。
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We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.
为此,本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs,使用无血清培养基进行扩增、培养,用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定,对少突胶质系细胞表达部分营养因子的情况进行检测;采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测;将OPCs移植入成年SD大鼠玻璃体内,利用上丘逆行荧光标记技术,观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间;将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内,观察不同时期视网膜内髓鞘形成与分布特点,分析髓鞘的超微结构,并观察眼内髓鞘形成对损伤神经节细胞的保护作用。
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Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.
基于以上两种重要疾病和研究组方向,本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作,累计筛选1万多个/次样品,发现10余个活性较强化合物;从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究,分离鉴定了58个化合物,发现6个新化合物和14个抗肿瘤活性化合物;通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究,发现它可能是通过降低G2期重要的转录因子E2F-1,致使细胞周期阻滞在G0/G1期,引起多发性骨髓瘤U266细胞凋亡;开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析,结果表明计算与实验一致。
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Results In 54 patients, 43 cases showed the masses with the first symptom, the percentage of which was 79.6%. The rate of stage II ~ III ( including 44cases) was 81.5%. The rate of 32 cases with accompanied diseases was 59.3%. 38 cases were breast cancer modified tradical mastectomy (70.3%), 12 cases were mammectomy(22.2%), 4 cases were partial mammectomy(7.4%). 23 cases were post-operation chemotherapy(42.6%), 12 cases were radiotherapy(22.2%), 43cases were Endocrine therapy(79.6%).
结果 54例患者中,以肿块为首发症状的有43例,占79.6%,Ⅱ~Ⅲ期病例44例,占81.5%,32例同时存在伴随疾病,占59.3%,其中行改良根治术38例(70.3%),单纯乳房切除术12例(22.2%),乳房部分切除术4例(占7.4%),术后化疗23例(42.6%),放疗12例(22.2%),内分泌治疗43例(79.6%),无手术死亡。
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Results Women were more often affected than men(1.83:1) and 53 patients were older than 40 years. The involved parts were as follows: eyelid 58 cases (89.2%), mouth and mandibula 46 cases(70.8%),neck 17 cases(26.2%). The symptom at onset were blepharospasm 42 cases(64.6%) or oromandibular dystonia 17 cases(26.2%).In part of the patients, cranial MRI examination could find focus in brainstem or basal ganglia. Among 37 patients treated with oral drugs, 4 cases were almost cured, 16 cases improved and 17 cases showed no effect. While in 15 patients treated through chiostridium botulinum toxin injection, 5 were almost cured, 9 improved and 1 had no effect.
结果 男女患病比率为1:1.83,40岁以上发病53例,受累部位依次为眼睑58例(89.2%)、口-下颌46例(70.8%)、颈部17例(26.2%);首发症状以眼部不适、眼睑痉挛最多(42例,占64.6%),口-下颌肌张力障碍次之(17例,占26.2%);14例行头MRI检查者8例发现脑干或基底节区病灶;口服药物治疗37例,显效4例,有效16例,无效17例;15例行肉毒毒素局部注射者显效5例,有效9例,无效1例。
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After 24 hours, all rats were anesthetized again and their left common carotid arteries were cannulated to facilitate blood withdrawal for serum sample. The contents of tumor necrosis factor-a and interleukin-6 (IL-6) in serum were determined before and after experiment by ELISA; the activity of superoxide dismutase and content of malondialdehyde were determined by xanthinoxidanse method and modified TBA microdetermination. The content of NO2(superscript -)/NO3(superscript -), and activity of nitric oxide synthase and inducible NOS were determined by nitrate reductase method, and chemical chromogenic reaction, respectively; the immunoglobulin G, IgA, IgM and complement 3 (C3) and C4 were determined by immunoprecipitation method before and after experiment.
实验评估:手术创伤后24h进行采用酶联免疫吸附法测定实验前后血清肿瘤坏死因子α、白细胞介素6含量;应用黄嘌呤氧化酶法测定超氧化物歧化酶活性、改良TBA微量法测定丙二醛水平;以硝酸还原酶法、化学显色法分别检测NO2/NO3浓度及一氧化氮合酶、诱导型一氧化氮合酶活胜;以免疫比浊法经全自动生化分析仪检测血清免疫球蛋白IgG、IgM、IgA及补体C3、C4含量。
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In this article, three kinds of prealloy powder were developed by water atomization method. The base attribute, pattern, phase composition, differential thermal analysis of powder were researched. Sintered density, hardness, bend strength, Young's modulus were researched as hot pressed sintering temperature changing. By using abrasive wearing equipment upbuilt by the authors, the wear resistance were determined. Green-pressing density, simulated green-pressing strength and bonding momental of high frequency brazing were assayed. SEM corrosion structure and SEM appearance of fracture were observed.
本文采用水雾化法开发了三种预合金代钴粉末:对粉末的基本属性、形貌,粉末的相组成,粉末的差热性进行了分析研究;测定了它们的烧结密度、硬度、抗弯强度、弹性模量随热压烧结温度变化的规律;采用作者自行建立的磨料磨损试验方法,测定了它们的质量磨损百分率;测定了它们的冷压生坯密度、模拟生坯强度以及高频钎焊的结合力矩;观察了热压烧结SEM腐蚀组织和SEM断口形貌。
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objective to study the ct diagnosis of ureteral calculus at the distal end of ureter.methods 82 cases of ureteral caculus were examined by ct scanning.all cases were proved by clinical therapeutic results.results ureteral calculuses were showed as high density image in ct scanning.the ct values of the ureteral calculuses less than 7.5mm in diameter were beyond 83hu.the detection rate by ct was 96.3%.in all the 82 cases,ureteral with calculuses were found dilated and 69 cases with nephrohydrosis.in 13 cases with no nephrohydrosis,caculuses less than 3.8 mm in diameter were found.conclusion ct is the most valuable way to diagnose ureteral calculus at the lower end of ureter.appropriate methods of ct scanning are essential.
目的 回顾性分析输尿管膀胱入口处结石的ct诊断结果,评价其ct检查的临床应用价值。方法分析82例输尿管膀胱入口处结石的ct检查结果,所有病例均经尿道排石证实。结果输尿管膀胱入口处结石具有特征性的ct表现,即圆形或枣核状钙化高密度影,结石直径<7.5mm,ct值≥83hu,ct确诊率96.3%。82例均发现病侧输尿管增粗,69例发现病侧肾盂少量积水,肾盂未见积水的输尿管膀胱入口处结石13例,其结石直径<3.8mm。结论 ct检查输尿管膀胱入口处结石具有准确、无创、直观等优点,是诊断输尿管膀胱入口处结石不可替代的检查方法。
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Methods 120 SD rats were divided randomly into blank group, model group, Baoliezhi control group and Qianliening granule groups with different doses. The models were established with hypodermic injection of testosterone propionate at 4 mg/kg after orchiectomy, at the same time, these rats were gastric perfused with different drugs respectively for 30 days. The body weight, prostate weight, prostate index, morphological changes, the level of T and E2 were measured. Results The body weight in model group was significantly lower than that in control group (P.05), the prostate weight and index were obviously higher than that in control group (P.01). The prostate weight and index were decreased significantly in Qianliening granule groups with meddle and high doses (P.01), the level of testosterone was increased significantly and E2 was decreased obviously in Qianliening granule group with high doses.
将雄性SD大鼠60只,随机分成空白组、模型组、保列治治疗对照组、前列宁颗粒低、中、高剂量组,采用丙酸睾丸酮皮下注射引起大鼠前列腺增生模型,连续给药30d后,观察6组动物体重前列腺湿重前列腺指数前列腺组织形态血清睾酮雌二醇(E2)水平的变化结果模型组大鼠体重较正常组明显减轻(P.05),而前列腺重量和前列腺指数明显升高(P.01);前列宁颗粒中、高剂量组的前列腺重量和前列腺指数明显下降(P.01);前列宁颗粒高剂量组可明显升高大鼠血清T水平,降低E2水平,使T/E2比例升高。
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The new opinion and mechanism that the simultaneously adsorption of complex pollution of both organic and heavy metals will be achieved on soil modified with amphoteric surface modifying agent, which have both charged group and organophilic group on one ASMA molecule, were proposed at first time, and its rationality was testified at the same time; The 50% was the critical CTMAB modifying ratio of soil CEC at which the organophilic bond modifying mechanism was emerged; The thermodynamic characteristics of adsorption of OPT and HMP on OMS were discovered ; The study revealed the surface peculiarity of OMS that the modifying agent were distributed at uneven scatter mode and multi-characters surface were coexisted on surface of OMS, revealed the adsorption peculiarity of OMS that multi-mechanisms were coexisted, confirmed the unequal charge amount modifying mode of OMS in which dual-modifying mechanism of both ion exchange and OPB were processed at the same time, and confirmed that the adsorption hobby to CTMAB was higher than that to Ca2+ on Lou soil.
首次提出了用两性表面修饰剂对土壤进行修饰改性,利用两性表面修饰剂所具有的荷电基团和疏水基团达到对土壤中有机、重金属污染物同时吸附的思路和机理,并通过实验证实了所提设想的合理性;发现了土壤CEC值的50%修饰比例是CTMAB开始显现以疏水键机制对土土样表面进行修饰的临界点;揭示了有机修饰改性土对有机物、重金属吸附过程的热力学特征,填补了该项研究工作的空白;阐明了修饰剂在改性土表面呈分散态的非均匀分布,改性土表面具有不同性质表面共存的表面特征和对污染物多机制共存的吸附特征,确定了阳离子表面修饰剂在土表面的修饰是一种离子交换和疏水键结合两种机制相伴并行且为非等电荷量交换的修饰反应模式,以及土具有对CTMAB的吸附偏好性远大于对钙离子吸附偏好性的特征。
- 相关中文对照歌词
- Hela Världen För Mig
- Din Första Kyss
- Sov Gott
- Fernando
- En Livstid I Krig
- Poltava
- Gott Mit Uns
- Ett Slag Färgat Rött
- Karolinens Bön
- Baksmälla
- 推荐网络例句
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Objective To investigate the relationship between ovum fertilization rate and sperm morphology, acrosome reaction in IVF and to explore the influence of parameters of sperm on fertilization rate.
目的 研究体外受精(in vitro fertilization,IVF)中精子形态、顶体反应与卵子受精率之间的关系,分析精子各参数在IVF受精率方面的参考意义。
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A: I hear Mr. White wants to run for the Presidency!
我听说怀特先生要参加总统竞选!
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It was a blowy day soon after Hilda had gone, that Mrs Bolton said: 'Now why do not you go for a walk through the wood, and look at the daffs behind the keeper's cottage?
希尔达走了不久以后的一个刮风天,波太太对她说:你为什么不到树林里去散散步,到守猎人的村舍后边去看看野水仙?