查询词典 were it not for

The hFCECs were cultured by sticking tissues piece method that the cornea were divided into the limbus and central tissue piece and digestive method,respectively.For digestive method,the digestive juice of 5mg/ml DispaseⅡ+0.25%trypsin was chosed.D/F12 with 10%fetal bovine serum and 100 U/ml penicillin and streptomycin were used in this study,the culture condition was 37℃and 5%CO2,and culture medium changed every three days2、Passage of hFCECsAfter more than 80%confluenced,cells from corneal limbus hFCECs were digested for 5-15min with different concentrations of trypsin/EDTA at 37℃,and then passaged at a ratio of 1:2.The cells were subcultured on empty plates,mouse 3T3 fibroblast feeder layer,fetal corneal stromal cell feeder l ayer or HTK feeder layer respectively.And D/F12, mouse 3T3 fibroblast conditioned medium,fetal corneal stromal cells conditioned medium or HTK conditioned medium with 10%FBS and 100 U/ml penicillin and streptomycin were used respectively as the culture medium.And the culture medium were changed every three days as well.

方法一、人胎儿角膜上皮原代和传代培养1、原代培养严格无菌操作获取人胎儿角膜片，采用组织块贴壁法、酶消化法原代培养人胎儿角膜上皮细胞。2、传代培养角膜缘部的细胞生长融合达80%以上，不同稀释浓度的胰酶/EDTA消化液消化传代分别接种于空板、小鼠3T3成纤维细胞饲养层、胎儿角膜基质细胞饲养层及HTK饲养层，培养液分别采用D/F12、小鼠3T3成纤维细胞条件培养液、胎儿角膜基质细胞条件培养液、HTK条件培养液。

The results showed: when TLC solvent system was: chloroform - methanol - diethyl amine = 90:9:1, alkaloids from lotus leaves were well isolated, and five components were isolated and identified by spraying Dragendorff reagent. The optimal conditions of HPLC solvent system were: methanol- water - diethyl amine = 75:25:0.0125. Under the conditions, components of alkaloids from lotus leaves were separated very well, and ideal RP-HPLC peaks were obtained.5、Methods of High Speed Counter Current Chromatography and preparative High Performance Liquid Chromatography applied to identify and purify alkaloids from lotus leaves were set up. When solvent system of HSCCC was: chloroform - methanol - water (pH=4.00)= 4:3:2, speed of chromatogram was 700rpm, flow speed of the mobile phase was 2mL/min, four pure components relatively were attained by isolation and purification.

结果表明：薄层层析溶剂系统为氯仿：甲醇：二乙胺=90：9：1，使荷叶中的生物碱达到了较好的分离效果，并用改良的碘化铋钾试剂喷雾显色，共分离鉴别出了5种荷叶生物碱；分析型HPLC分析检测的较优溶剂系统为甲醇：水：二乙胺=75：25：0.0125，使荷叶生物碱的各个组分达到了基线分离，并获得了较好的峰形。5、建立了高速逆流色谱和制备型HPLC技术分离纯化荷叶生物碱的具体方法。H(来源：ABC46论文网www.abclunwen.com)SCCC法在溶剂系统为氯仿：甲醇：pH4.00的水=4：3：2，色谱仪转速为700rpm，流动相流速为2mL／min的条件下，分离纯化得到了4个纯度较高的化合物。

The F-P structure, especially the thermal-strain mechanism of thermal bimetal sheets were investigated deeply in the paper, the Finite Element Analysis results for both the "four nodes" and the "ring" confinements were given; the size of the ring hole can be simply changed to agilely meet the requirement of the different measurement range; the materials match and the parameters selection of the thermal bimetal sheets were analyzed and discussed, and some available suggestions were given; the F-P temperature sensors with the two developed bimetal sheets confinement mechanism were designed and fabricated, the practical measurements were carried out, and the experiment results were analyzed and discussed.

文中对所提出的F-P腔结构，特别是热双金属片的应变机制进行了较系统深入的研究，给出四点约束和环状约束具体有限元分析结果；提出的环状约束可以简单改变环孔大小灵活适应不同量程的需要；对热双金属片材料配对和参数选择进行了分析讨论，并给出有益的建议；实际制作了两种约束机制的光纤热双金属片F-P腔温度传感器，进行了相关实际测试，并对实验结果进行了讨论。

Methods ①The spiral organ of corti of 10 mice (20 ears) were taken, and divided into 2 groups randomly (n=10), cultured in 0.1 mol·L-1 gentamicin and the metabolite of 0.1 mol·L-1 gentamicin for 48h, then the number of hair cells remain was recorded;②The spiral organ of corti of 10 mice were taken (20 ears), and divided into 2 groups randomly (n=10), 3% CaCl2 and 0.1 mol·L-1 GM were mixed in the culture medium and the number of hair cells remain in the two medium mentioned above were recorded;③The spiral organ of corti of 10 mice were taken (20 ears), divided into 2 groups randomly (n=10), 3% MgSO4 to 0.1 mol·L-1 GM were mixed in the culture medium, the number of hair cells remain in the two medium mentioned above were recorded.

①取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM代谢产物的培养液中培养48 h后，记数残留的耳蜗毛细胞数；②取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM+3% CaCl2的培养液中培养48 h后，记数残留的耳蜗毛细胞数；③取10只小鼠的耳蜗螺旋器（20个），随机分为2组（n=10），分别在含0.1 mol·L-1 GM与0.1 mol·L-1GM+3% MgSO4的培养液中培养48 h后，记数残留的耳蜗毛细胞数。

Ltd. That case, I think, authorizes four propositions first, that, where a contract is ex facie illegal, the Court will not enforce it, whether the illegality is pleaded or not; secondly, that, where, as here, the contract is not ex facie illegal, evidence of extraneous circumstances tending to show that it has an illegal object should not be admitted unless the circumstances relied on are pleaded; thirdly, that, where unpleaded facts, which taken by themselves show an illegal object, have been revealed in evidence (because, perhaps, no objection was raised or because they were adduced for some other purpose), the Court should not act on them unless it is satisfied that the whole of the relevant circumstances are before it; but, fourthly, that, where the Court is satisfied that all the relevant facts are before it and it can see clearly from them that the contract had an illegal object, it may not enforce the contract, whether the facts were pleaded or not.

第1：不论状书有否提及非法之问题，如有关合约表面上已属非法，法庭不会强制执行该合约之任何条款；第2：如合约表面上并非不合法，在状书中未有提及之情况下，法庭不应接纳外来之环境证据去显示有关合约有不合法之目的；第3：如显示合约有非法目的之事实，在状书中未有提及，但由於未遭反对或其他原因已向法庭提出，法庭亦不应考虑该等事实，除非法庭信纳已经掌握了与案有关之全部事实；第4：如法庭信纳已经掌握有关之全部事实，而该等事实清楚显示有关合约有非法目的，无论状书是否有提及该等事实，法庭都不会执行合约内之条款。

The levels of schistosome circulating cathodic antigen in serum and urine were detected parallelly by McAb-Dot-ELISA. In 81 acute cases, the positive rates in serum and urine were 96.30% and 66.67%(P＜0.01) but the combined positive (positive in serum and／or in urine) rate reached 100%, while in 109 chronic cases, they were 77.06% and 10.09%(P＜0.01) but the combined positive rate was not increased. In 100 healthy individuals from non-endemic areas, no positives were detected in serum and urine with false positive rate of 0%. The cross reaction rates in serum and urine of 51 cases with clonorchiasis were 9.43% and 0% but all were 0% in 48 cases with ancylostomiasis.

提要 应用McAb-Dot-ELISA平行检测血吸虫病人的血清和尿样(浓缩20倍)中日本血吸虫肠相关趋阴极循环抗原。81例急性血吸虫病患者的血清和尿样的阳性率分别为96.30%和66.67%，而血清和尿样组合检测的阳性检出率提高至100%。109例慢性血吸虫病患者的血清和尿样的阳性检出率分别为77.06%和10.09%，组合检测的阳性检出率没有提高。100份健康人的血清和尿样的假阳性率均为0%。54例华支睾吸虫病患者的血清和尿样的交叉反应率分别为9.43%和0%。48例钩虫病患者的血清和尿样的交叉反应率均为0%。

Results 3 cases of lesions were in the gap of"V"shape between iliac muscle and musculus psoas major on the coronal sections in the lower right abdomen closely adjacent to the cecum,which were narrowed. The lesions showed long even T1 and T2 signals similar to mucous wall particularly in T2WI image. The walls of cyst were thin and even. The cysts were round or oval in shape on transections.The cyst were cucurbits in shape in 2 cases and long cannular in a case on coronal sections and arrowy sections and borderlines of the cysts were clear. The terminal of the cysts showed taper in 2 cases. In addition, cecum polypus was found at the placket of the appendix in a case which was not shown on MRI image.

结果 3例病变均位于右下腹，冠状面位于髂肌和腰大肌形成的"V"形间隙内，与盲肠关系密切，盲肠呈外压改变；呈均匀长T1、长 T2信号；壁薄且均匀，信号与肠管壁相似，于T2WI显示较明显；于横断面分别呈圆形、类圆形及椭圆形，冠状面及矢状面2例显示呈"葫芦"状、1例呈长管状，3例末端均呈"桃尖"样变；边界均清楚。1例于阑尾开口部发生盲肠息肉，MRI未能显示。

Trichogramma showed high control efficacy on cotton bollworm eggs after three times of release both in the second and in the third generation bollworm periods. The parasitism on the second and third generation bollworm eggs were 66.0%-70.7% and 76.0%-81.2%, respectively, which were significantly higher than that in the control (second generation: 7.0%-12.3%, third generation: 5.2%-7.4%). And the injury rate of cotton buds and bolls in the second and third generation bollworm were 2.2% and 3.1%, respectively, which were significantly lower than that in the control (second generation:8.5%, third generation: 20.9%). In the second generation bollworm period, number of bollworm eggs or larvae in the Trichogramma released cotton field did not differ to that in the chemically controlled field. But in the third generation, there were significantly less bollworm eggs and larvae in the Trichogramma released cotton field. There were more predators in the Trichogramma released cotton field. These results indicated that cotton boll worm in the transgenic insect-resistant cotton field can he effectively controlled by mass-releasing of T.

结果发现：棉铃虫卵在棉株不同部位的分布差异较大，2代棉铃虫卵主要分布在叶正面、叶背面和茎尖，3代棉铃虫卵主要分布于蕾铃；在放蜂田，赤眼蜂对棉株不同部位棉铃虫卵的寄生率差异较大，2，3代棉铃虫时期对叶正面、叫背面、茎尖、蕾铃的棉铃虫卵寄生率均较高，对叶柄和茎杆处卵的寄生率较低；并以3代棉铃虫时期的寄生率较高；在2，3代棉铃虫时期分别连续释放3次赤眼蜂，对棉铃虫具有很强控制作用，2，3代棉铃虫卵的被寄生率分别为66.0%~70.7%和76.0%~81.2%，均显著高于化防田的自然寄生率（2代：7.0%~12.3%，3代：5.2%~7.4%）；蕾铃被害率分别为2.2%和3.1%，均显著低于化防田（2代：8.5%，3代：20.9%；2代棉铃虫时期，放蜂田和化防田的棉铃虫卵量和幼虫数量差异均不显著，但3代棉铃虫以后，放蜂田棉铃虫卵量和幼虫数量均显著低于化防田，且放蜂田的捕食性天敌数量显著高于化防田。

The results showed:among the 7 isolates, five isolates of HY3、GY1－3、ZJ1－1、HP1、FC3 had same colony shape, irregular shape, liquidlike, slimy, opacity with smooth surface;the other two isolates had same shape, irregular shape, dry, opacity with coarse surface. By inoculating eucalyptus with the 7 isolates, the plants were infected apparently, and the young plants of eucalyptus in control experiment with tap water were not infected. By cultivating eucalyptus cuttings with the bacterial suspensions without EPS, the incidence of disease was very distinct,but compared with the former bacteria suspension,the incidence of disease has decreased at different degrees. By screening out two isolates of strong pathogenicity and two isolates of weak pathogenicity from the 7 isolates,making the bacterial suspensions with them to inoculate the young plants of eucalyptus, two treatments of cutlings and ramets with rats were set with 5 repetitions in every treatment, the results of data analysis showed: for the cutlings, the bacterial contents in upper and middle parts、upper and lower had significant difference;for ramets with roots, the bacterial contents in upper, middle parts, lower had significant difference between each other; For both the cutlings and ramets with roof, the bacterial contents in xylem and phloem had significant difference. The interaction between vertical and horizontal parts for the bacterial content had significant difference. For the two isolates of HY3 and 93B which were screened out at last,their activities of the cellulase were: 1.955ug/ and 1.288ug/ respectively, and had significant difference; the activities of pectase were: 1.325 ug/and 1.24ug/ respectively, and had no significant difference. The content of EPS extracted from the two isolates of HY3 and 93B was very different: 7.08x10-8ug/cell and 5.17x10-8ug/cell.

结果显示：7个菌株中，其中5个菌株HY3、GY1－3、ZJ1－1、HP1、FC3的菌落形态相同：不规则形状、流体、粘性、不透明、表面光滑；另外2个菌株93B、GN1菌落形态相同：不规则形状、干燥、不透明、表面粗糙；用7个菌株接种剪根桉树苗，发病情况非常明显，而自来水对照实验中桉树苗却不发病；无EPS菌悬液培养桉树剪根苗，发病率也很明显，但是相比原菌液，则发病率有不同程度的下降；从7个菌株中间筛选出来2个强致病性菌株和2个弱致病性菌株，用它们配制菌悬液培养桉树苗，设置剪根和不剪根两个处理，每个处理设置五个重复，数据分析结果显示：对于剪根苗，上部和中部、上部和下部的含菌量有显著的差异，中部和下部含菌量差异不显著；带根苗，上部、中部、下部含菌量彼此之间差异显著；不管是剪根苗还是带根苗，木质部和韧皮部含菌量之间的差异都非常显著；上中下与木韧交互作用中，含菌量差异非常显著；最后筛选出来的强弱2个菌株HY3和93B，它们的纤维素酶活性分别为：1.955ug/和1.288ug/，具有显著的差别；果胶酶的活性分别为：1.325 ug/和1.24ug/，没有显著的差别，而且HY3和93B两个菌株细胞分泌的胞外多糖的含量差异很显著，分别为：7.08×10-8ug/cell和5.17×10-8ug/cell。

Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库；(2)经蓝白菌落筛选和斑点杂交，正向消减文库获得307个阳性克隆，反向消减文库获得78个阳性克隆；(3)正向消减文库挑选55个克隆成功测序，经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95％～100％)，这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等；(4)反向消减文库挑选31个克隆成功测序，经同源性检索和比对最终确定差异表达基因片段16个，其中15个与人类全长基因具有很高相似性(95％～100％)，包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性，表明它可能是未被克隆的人类基因EST片段。

These rules reflect some inherent feature of self and nonself, and they are the basic detection criterion of GECISM.

这些规则反映了"自我"和"非我"的内在特征，是GECISM进行"非我"检测的判据。

Until today, the media from China and the outside world still uses the term "Post 89". Regardless to whether it is for commercial gimmick or convenience, everybody still remember "Post 89", a term which carries a strong Cantonese accent.

时至今日，国内外艺术媒体还是在沿用&后八九&这个称谓，不管当初是为了商业噱头还是容易上口好记，反正大家现在是记住了&后八九&这个广东口音很重的词。