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The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened,and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.

应用细胞融合制备46株杂交瘤，对其中10株进行克隆化，获得bFGF特异单抗16株，2株针对融合蛋白；应用该单抗建立了0.1ng/孔灵敏度的ELISA，0.5ng/孔敏度的斑点ELISA；用Western-blotting鉴别了经改造不同工程菌蛋白表达，指导重组工程菌改造；用3T3细胞培养研究了单抗抑制bFGF生物学活性的剂量和特点；合作研究了20例肝癌、肝癌细胞株和正常肝组织，发现前者bFGF高表达；应用单抗偶联CNBr-sepharose 4B建立了小量免疫亲和层析纯化bFGF，纯度达到95%；发现神经性肌萎缩患者血清中含有高滴度的bFGF抗体，已有10多家单位引用单抗或进行合作。

At 4 weeks following implantation, cells on the surface of coralline hydroxyapatite were found and connective tissues were seen in the material pores in the experimental group. Cells on the coralline hydroxyapatite were observed only in the control group. At 8 weeks, new bone formation was detected on the surface of coralline hydroxyapatite; bony tissue deposition and a few chondroid tissues were found in the pores or surrounding the pores in the experimental group. A few fibrous connective tissues were observed in the control group. At 12 weeks, abundant mature woven bone was detected on the surface of coralline hydroxyapatite; medullary cavity-like structure and vessels were found in some regions in the experimental group. No new bone or bony tissues were found in the control group.

植入材料后4周，实验组可见珊瑚羟基磷灰石表面有细胞生长，孔隙内有结缔组织长入；对照组仅见珊瑚羟基磷灰石表面有细胞生长。8周时珊瑚羟基磷灰石表面有新生骨形成，孔隙内和孔隙边缘可见骨样组织沉积和少量软骨样组织形成；对照组仅见少量纤维结缔组织长入。12周时珊瑚羟基磷灰石材料表面有较多成熟编织骨形成，部分区域可见髓腔样结构形成，并有血管长入；对照组仍未见新骨及骨样组织形成。

Results About 39 cases were diagnosed as benign tumors,majority of which were demoid cysts.The another one case was borderline mucinous cystadenoma.Eight cases were diagnosed by ultrasound examination during the first trimester(53.33 %),4 had complication to do emergency operations,3 were caused by torsion,one by rupure.Serum samples for CA125 estimation were obtained from 3 before operations.AS a result,2 cases were elevated,one of which was borderline mucinoers cystadenoma.38 cases were full-term birth.

结果 妊娠期良性肿瘤39例，占97.5%，以生殖细胞肿瘤多见，黏液性囊腺瘤交界型1例；孕早期超声诊断8例，占孕期发现总例数的53.33%(8/15)；有4例出现并发症并行急诊手术，扭转3例，破裂1例，术前3例行CA125检查，2例升高，其中1例为黏液性囊腺瘤交界型；足月分娩38例，占95.00%。

Results Of 10 cases with internal impingements,MR showed infraspinatus abnormalities were 8 cases.Supraspinatus abnormalities were 4 cases.Labral abnormalities were 9 cases.Posterosuperior humeral head damage were 10 cases.10 cases with subacromial impingement.MR showed infraspinatus abnormalities were 2 cases.Supraspinatus abnormalities were 8 cases.Labral abnormalities were 0 case.

结果 10例内在撞击综合征MR表现为冈下肌腱异常者8例，冈上肌腱异常者4例，盂唇异常者9例，肱骨头后上部异常10例。10例肩峰下撞击综合征MR表现为冈下肌腱异常者2例，冈上肌腱异常者8例，盂唇异常者0例，肱骨大结节异常8例。

Results MRI appearances of acoustic neuromas were:（1）The turmor's growth round the internal auditory canal,the Ⅶ,Ⅷ nerves affected side were thickened than that of opposite side.There was no marked demarcation between the main bodies of the tumors and the affected nerves.The connection between tumors and affected nerves could be showed at contrast enhanced imaging.（2）The tumors were of hypo or isohypo intensity on T1 weighted images and hyper or iso hyper intensity on T2 weighted images.（3）After Gd DTPA administration the tumors were homogeneously or inhomogeneously or circularly enhanced.（4）The margins of the tumors were smooth and well delineated.（5）The affected internal auditory canal usually became shorter in depth and larger in posterior anterior diameter.（6）MRI appearances of micro acoustic neuromas were that the affected Ⅶ,Ⅷ nerves were normal in size or nodularly thickened and the tumor reveaed nodular or point enhancement at contrast enhanced MRI.

结果 听神经瘤的MRI表现主要为：（1）肿瘤以内听道为中心生长，第Ⅶ、Ⅷ神经束较对侧增粗，与肿瘤主体多无明确分界，增强后可见两者相连，颇为特征；（2）肿瘤在T1加权图像上呈低信号、略低信号或低等混合信号，T2加权呈高信号或高等混合信号；（3）注射Gd DTPA后肿瘤呈均匀、不均匀或环状强化；（4）肿瘤边界清晰，边缘光整；（5）绝大多数肿瘤患侧内听道前后径扩大，深径缩短；（6）微小听神经瘤的MRI表现为第Ⅶ、Ⅷ神经束正常或局部结状增粗，造影后呈结节状或点状增强。

Salix matsudana, Ulmus pumila, Quermus mongolica, Populus alba×P.beroliensis and Betula platyphlla are frontal 5 tree species with weight value 90.37, 90.12, 88.12, 87.55 and 86.2 respectively, were used for farmland protection forest; Acer negundo, Salix matsudana, Juniperus rigida, Fraxinus mandshurica and Ulmus pumila are frontal 5 tree species with weight value 78.14, 76.92, 75.96, 74.72 and 74.56 respectively, were used for urban protection forest; Ulmus pumila, Salix matsudana, Populus alba×P.beroliensis, Acer negundo and Larix gemelinii are frontal 5 arbor tree species with weight value 80.07, 79.07, 77.5, 72.42 and 71.67 respectively, were used for road protection forest; Salix matsudana, Populus alba×P.beroliensis, Ulmus pumila, Larixgemelinii and Betula platyphlla are frontal 5 tree species with weight value 92.37, 88.72, 86.62, 85.82 and 85.7 respectively, were used for river protection forest; Fraxinus mandshurica, Larix gemelinii, Populus alba*P.beroliensis, Ulmus pumila and Salix matsudana are frontal 5 tree species with weight value 89.61, 8.35, 87.12, 85.56 and 80.15 respectively, were used for wood forest; Cornus alba, Prunus tomentosa, Primus triloba, Syringa obalata and Sambucus williamsii are frontal 5 shrubs with weight value 82.93, 81.23, 80.85, 78.38 and 77.78 respectively, were used for landscape forest; and Acer negundo,Malus baccata, Sorbus pohuashanensis, Prunus maackii and Prunus padus are frontal 5 arbors with weight value 82, 81.52, 80.3, 79.62 and 78.9 respectively, were used for landscape forest; Prunus salicina, Prunu

根据哈尔滨地区不同林种的功能要求，给出了不同指标的期望值，通过查询系统可以得到旱柳、榆树、蒙古栎、银中杨、白桦为符合农田防护林的前五位的树种，权重值分别为90.37、90.12、88.12、87.55、86.2；糖槭、旱柳、杜松、水曲柳、榆树为符合城市防护林的前五位的树种，权重值分别为78.14、76.92、75.96、74.72、74.56；榆树、早柳、银中杨、糖槭、兴安落叶松为符合道路防护林的前五位的乔木树种，权重值分别为80.07、79.07、77.5、72.42、71.67；旱柳、银中杨、榆树、兴安落叶松、白桦为符合河岸防护林的前五位的树种，权重值分别为92.37、88.72、86.62、85.82、85.7：水曲柳、兴安落叶松、银中杨、榆树、旱柳为符合用材林的前五位的树种，权重值分别为89.61、88.35、87.12、85.56、80.15；红瑞木、毛樱桃、榆叶梅、紫丁香、接骨木为符合风景林前五位的灌木树种，权重值分别为82.93、81.23、80.85、78.38、77.78，糖槭、山丁子、花楸、山桃稠李、稠李为符合风景林前五位的乔木树种，权重值分别为82、81.52、80.3、79.62、78.9；李、杏、文冠果、山楂、野梨为符合经济林的前五位的树种，权重值分别为88.85、80.88、73.25、69.35、63.77。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度，然后每个角膜被分成两半，共48个半片角膜，再分成4组，每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数；12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变；12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性；12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

Methods: 81 cases with elderly NSCLC were divided into two groups randomly,43 cases in the treatment group were administrated with Sinoactionomycin 6g/kg (1~4 day) combining card platinum 300mg/m2 (1st day), glucoside (VP16) 100 mg (1~4 or 5days) intravenously.38 cases in the control group were only treated with CBP and vp16 and the dosage were same with that of treatment group.21~28 days were taken as a treatment cycle and the curative effects were studied after 2~3 cycles.The relievable and survival periods were investigated as well.

选取老年NSCLC病例81例，随机分治疗组43例给予新福菌素6μg/kg（第1~4d），卡铂300mg/m2（第1d），依托泊苷（VP16）100 mg（第1~4或5d）静脉滴注；对照组38例单纯使用CBP和VP16，剂量和方法同治疗组，21~28d为一个疗程，2~3个疗程评价疗效，随访缓解期和生存期。

These rules reflect some inherent feature of self and nonself, and they are the basic detection criterion of GECISM.

这些规则反映了"自我"和"非我"的内在特征，是GECISM进行"非我"检测的判据。

Until today, the media from China and the outside world still uses the term "Post 89". Regardless to whether it is for commercial gimmick or convenience, everybody still remember "Post 89", a term which carries a strong Cantonese accent.

时至今日，国内外艺术媒体还是在沿用&后八九&这个称谓，不管当初是为了商业噱头还是容易上口好记，反正大家现在是记住了&后八九&这个广东口音很重的词。