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The experimental conditions were as follows: C18 chromatographic column, mobile phase of MeOH-H2O (both include 0.1% methanoic acid), flow rate at 1.0 mL/min, gradient elution, detection wavelength at 290 nm and room temperature.

采用C18色谱柱，甲醇－水（含0.1％的甲酸）梯度洗脱；流速为1.0mL/min，检测波长290nm；柱温为室温。

Light source selection of print, in the light source is a notable issue, iodine gallium lamp is a type of light source are appropriate for plate burning, its high continuous use, the emission wavelength of microspectroscopic spread over 350 MW to 450nm to 417nm to the highest Summit, for most printing plate photoptic layers.

平源 晒版洋平源的拔存是个不值得夺纲的题纲，碘镓灯是一栽得当于晒版的平源，其发平成果矮，可不停搁置，其发放的平谱波长漫衍在350～450nm边界边，以417nm处为最矮峰，差不小都印版感平层灵验。

For example: peripherin-layer is made of nuphtheroquinon-xylenediol azide-anti-alcohol (microspectroscopic catalysical sensitivity range of up to 320 ~ 460nm), PS print, when you choose to use iodine gallium light, exposure time, simply by 20s with high pressure mercury lamp above the 30s, because although the two lamps and the spectral 300 ~ 460nm scope, but sulfonylated spetroscopy gallium light emission from 400 nm wavelength in close to the section of the distribution of light energy and light energy than much of high pressure mercury lamp, uniform is also much larger, and with this kind of formulation-peripherin-catalysical spetroscopy, emiting anastomasis sensitivity more productive.

感平层是用邻二叠氮醌型感平剂（平谱不分平感量边界为320～460nm）的PS版，晒版时若采取碘镓灯，曝平工夫只需20s把握，用矮压水银灯则给30s以上，这是因为：虽然两栽灯具的平谱成不分都在300～460nm边界边，但磺镓灯不收射的平谱洋挨近400nm波不幼段的平能量的不分布及平能量比矮压水银灯平均得众也不小得众，并且与这栽感平剂型的平谱不分平感量更为吻不分，不收平效果也更矮的原由。

MethodsHPLC was used.The stationary phase was Spherixorb C18column.The moble phase was acetonitrilewater(60∶40).The UV detection wavelength was 345 nm.

方法采用高效液相色谱法，C18色谱柱为固定相，乙腈水（60∶40）为流动相，检测波长为345 nm。

RP-HPLC is employed to determine the Isoflavones, with a C18 RP Col umn, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm.

反向高效液相色谱法进行含量测定，C18反相柱，MeOH CH3CN 0.1%H3PO4 为流动相，检测波长 260nm。

Methods The HPLC was performed on Lichrospher C18 (200 mm × 4.6 mm, 5μm) column. The mobile phase consisted of methanol and 0.02 molL^(-1) monobasic potassium phosphate buffer (60:40). The flow rate was 1.0 mlmin^(-1). The detection wavelength was set at 333 nm.

以汉邦Lichrospher C18 (200 mm×4.6 mm，5μm)为色谱柱，以甲醇-0.02 molL^(-1)磷酸二氢钾溶液（用磷酸调节pH值至4.2）60：40）为流动相，UV检测波长333 nm，流速1.0mlmin^

Methods: The HPLC with a C18 column (150mm×4.6mm, 5μm) was used. Methanol-0.05mol/L Potassium phosphate monobasic buffer solution-Acetic acid glacial (40:60:1) was used as mobile phase. The flow rate was 1.0mL/min, and the detection wavelength was at 333nm.

采用高效液相色谱法，用C18色谱柱(150mm×4.6mm， 5μm)，以甲醇-0.05mol/L磷酸二氢钾溶液-冰乙酸(40：60：1)为流动相，流速为1.0mL/min，检测波长333nm。

The chromatography conditions were as follows: methanol - 0.1mol/L monobasic potassium phosphate (50∶50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.

方法采用高效液相色谱法，色谱柱为Diamonsiltm C18柱；流动相：甲醇-0.1 mol/L磷酸二氢钾水溶液（50∶50）；流速：1.0 ml/min；检测波长：290 nm。

MethodsHigh Performance Liquid Chromatography was performed Diamonsil C18. The chromatography conditions were as follows: methanol - 0.1mol/L monobasic potassium phosphate (50∶50) as mobile phase, flow rate being 1.0ml/min and detecting wavelength was 290nm.

方法采用高效液相色谱法，色谱柱为Diamonsiltm C18柱；流动相：甲醇-0.1 mol/L磷酸二氢钾水溶液（50∶50）；流速：1.0 ml/min；检测波长：290 nm。

Methods The HPLC was performed on Shimpack CLCC18(250mm×4.6mm,5μm) column. The mobile phase consisted of metdhanol and 0.02molL-1 monobasic potassium phosphate buffer(65∶35). The flow rate was 0.9mlmin-1. The detection wavelength was set at 291nm.

以岛津Shimpack CLC C18(250mm×4.6mm，5μm)为色谱柱，以甲醇 0.02molL-1磷酸二氢钾溶液(用磷酸调节pH至3.0)(65∶35)为流动相，检测波长为UV291nm，流速为0.9mlmin-1。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。