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The separation of optical isomers of 2, 2'-dihydroxy-1, 1'-binaphthyl and 2, 2'-diamino-1, 1'-binaphthyl was studied by reversed-phase high performance liquid chromatography on a chiral stationary phase consisting of-hydroxypropyl-β-cyclodextrin (Cyclobond Ⅰ 2000 SP)using methanol/water as the mobile phase. The effects of mobile phase ratio, flow rate and column temperature on separation of optical isomers of DHBN and DABN were investigated.

在S-羟丙基－β-环糊精手性柱(CyclobondⅠ2000 SP)上用反相高效液相色谱法研究了2，2'-二氨基－1，1'-联萘和2，2'-二羟基－1，1'-联萘光学异构体的分离，考察了流动相组成、流速以及柱温等对联二萘胺和联二萘酚光学异构体分离的影响。

After nucleosides were extracted from urine on phenyl boronic acid affinity chromatography, the analysis was performed on a column (46 mm id×250 mm, 5 μm) at 22 ℃ using a linear gradient elution comprising 25 mmol/L KH2PO4 solution (pH 455) and 60% methanol in water with UV detection at 260 nm.

用该法测定了41例肠癌患者和52例正常人尿中15种核苷的含量(用核苷与肌酐的摩尔比表示，下同)，结果表明肠癌患者中有12种核苷的含量比正常人显著性增高(P 001)。以15种核苷的含量作为参量，结合主成分分析区分正常人和肠癌患者，对癌症病人的识别率达76%(31/41)。

After nucleosides were extracted from urine on phenyl boronic acid affinity chromatography, the analysis was performed on a column (4.6 mm i.d.X250 mm, 5 μm) at 22 ℃ using a lineargradient elution comprising 25 mmol/L KH2PO4 solution (pH 4.55) and 60% methanol in water with UV detection at 260 nm.

通过苯基硼酸亲和色谱法提取尿中核苷，在色谱柱(4.6 mm i.d.X250 mm， 5 μm)上以25 mmol/L磷酸二氢钾溶液(pH 4.55)和60%的甲醇水溶液作为流动相进行二元梯度淋洗，于22 ℃下进行反相色谱分离，260 nm处紫外检测。

To study the bioactive components from water soluble extract of the Chinese eaglewood, various column chromatography of Chinese eaglewood led to the isolation of ten compounds. Their structures were elucidated as 2,3-bis(3-hydroxyphenyl)- propan-1-ol (1), [(2R,3R)-3-(4-amino-3,5-dimethoxyphenyl)oxiran-2-yl]methanol (2), 8-chloro-4'- methoxy-5,6,7,3'-tetrahydroxy-2-(2-phenethyl)-5,6,7,8-tetrahydrochromone (3), 4'-methoxy-5,6,7,3'-tetrahydroxy-2--5,6,7,8-tetrahydrochromone (4), the isomer of 4 (5), 4'-methoxy-5,6,7,8-tetrahydroxy-2-(3'-hydroxyphenethyl)-5,6,7,8- tetrahydrochromenone (6), 4'-methoxyisoagarotetrol (7), 6-hydroxy-2-(2-hydroxy-2- phenethyl)-chromone (8), 6-hydroxy-2-(2-phenethyl)-chromone (9), 6-hydroxy-2-(4'- hydroxy-2-phenethyl)-chromone (10), and Guaiacylacetone (11) by means of spectral analysis and comparison with references.

为了进一步发现沉香中具有生理活性的化学成分，本文对国产沉香的水溶性成分进行了研究，从中分离得到11个化合物，通过红外、紫外、质谱、核磁共振(1H-NMR， 13C-NMR， COSY， HSQC， HMBC， ROESY)波谱解析，分别鉴定为：2，3-二(3-羟基苯基)-丙-1-醇(1)，[(2R，3R)-3-(4-氨基-3，5-二甲氧苯基)-2-氧化烯基]甲醇(2)，8-氯-4'-甲氧基-5，6，7，3'-四羟基-2-(2-苯乙基)-5，6，7，8-四氢色原酮(3)，4'-甲氧基-5，6，7，3'-四羟基-2-(2-苯乙基)-5，6，7，8-四氢色原酮(4)，化合物3的差向异构体(5)，4'-甲氧基-5，6，7，8-四羟基-2-(3'-羟基-2-苯乙基)-5，6，7，8-四氢色原酮(6)，4'-甲氧基异沉香四醇(7)，6-羟基-2-(2-羟基-2-苯乙基)色原酮(8)，6-羟基-2-(2-苯乙基)色原酮(9)，6-羟基-2-(4'-羟基-2-苯乙基)色原酮(10)和Guaiacylacetone (11)。

The watermelons, the citrullus lamtus or the stems and leaves thereof are taken as raw materials, and the extracting process comprises the following steps: firstly, the raw materials are washed clean and broken to separate the watermelon peel from the watermelon pulp for respectively extracting juice; the two juices are mixed, and micro-filtration and ultra-filtration are carried out to remove insoluble substances and macromolecular substances; and then reverse osmosis and nano-filtration are carried out to obtain the filtrate; the pH value of the filtrate is regulated and then a macroporous strong acid cation exchange resin column is added; and then ammonia water is taken as an eluant for isocratic elution; the eluent obtained is decompressed, distilled, deaminized, discolored, condensed and crystallized to obtain a white crystalline product of the natural L-citrulline extracted from the watermelons.

本发明方法以西瓜、打瓜或者其茎叶为原料，其过程为：首先将原料清洗干净，破碎后将西瓜皮西瓜瓤分开，分别进行榨汁；所得汁液合并，经过微滤和超滤除去不容物和大分子物质；然后再经反渗透和纳滤过滤；透过液调节pH值后通入大孔型强酸性阳离子交换树脂柱；然后以氨水做洗脱剂进行等度洗脱；将洗脱液减压蒸馏脱氨，脱色，浓缩结晶，得到西瓜提取天然L-瓜氨酸白色结晶产品。

A simple, selective and sensitive method was investigated for simultaneous determination of anions (SO2－4, Cl－, NO－3, F－) and cations (Na＋, NH＋4, K+, Mg2＋, Ca2＋) by single-column ion-exclusion-cation-exchange chromatography with conductometric detection. DL-malic acid-methanol-water was used as an eluent.

采用单柱离子色谱系统和电导检测的方法，首次以DL-苹果酸-甲醇水溶液为淋洗液，简便、有效地同时分离、检测了溶液中SO2-4、Cl-、NO-3、F-、Na+、NH+4、K+、Mg2+、Ca2+9种离子。

The ethanol extract was dissolved with HCl, the acidic extract was basified to pH9-10 with NH_3-water and extracted with CHCl_3 to give total alkaloids. five alkaloids were isolated with column chromatography and preparative thin-layer chromatography, and three of them were identified as dehydrocorydaline、 corynoline and dihydrosanguinarine on the basis of spectral analysis MS,~1H-NMR,~(13C-NMR and physicochemical properties. All of them were found for the first time from this plant.

为研究灰绿黄堇的化学成分，本文将灰绿黄堇乙醇提取物采取酸提取、碱游离后萃取的方法得到总生物碱，然后利用柱色谱、制备薄层色谱等方法分离、纯化了灰绿黄堇中5种生物碱，并利用IR、MS、~1H-NMR、~(13)C-NMR等方法鉴定了其中的3种，分别为去氢紫堇碱、紫堇灵、二氢血根碱，均为首次在此植物中发现。

Methods: In alkaline condition, reaction between amino acids and phenylisothiocyanate formed a derivant of phenylisothiocyanate-amino acid. For RP-HPLC, the Diamonsil C18 column 250 mm×4.6 mm,5 μm） and binary gradient elution were used. The mobile phase A was 0.1 mol/L sodium acetate solution-acetonitrile (93∶7） and the mobile phase B was acetonitrile-water（80∶20）. The detecting wavelength was 254 nm.

在碱性条件下，氨基酸与异硫氰酸苯酯反应，生成异硫氰酸苯–氨基酸的衍生物，采用 Diamonsil C18色谱柱（250 mm×4.6 mm，5 μm），用二元梯度洗脱方式，流动相A为0.1 mol/L乙酸钠–乙腈（93∶7），流动相B为乙腈–水（80∶20），检测波长254 nm。

To establish a MPLC method for the content determination of resveratrol in Polygonum cuspidatum Sieb. Et. Zucc, Analytical column SOURCE 5RPC ST 4.6/150 was used with a mixture of acetonitrile+water (30+70,volume ratio ) as mobile phase ,with a flow rate of 1ml/min and the UV detection wavelength was 306nm, The linear range was 0.6-1.6mg/ml (r=0.9919). The average recovery of prodatin was 103.0%-90.4%(RSD=7.21%). The method is convenient and accurate. Result indicated a reverse phase chromatography media based o n polystyrene/divinyl benzene has excellent resolution for resveratrol in cuspidatum. P rovide theoretical support for process development.

色谱条件采用SOURCE 5RPC ST 4.6/150预装柱，以乙腈/水(体积比30/70)溶液为流动相，流速1ml/min、检测波长为308nm，结果表明，白藜芦醇在0.6～1.6mg/ml的浓度范围内峰面积积分值与浓度的相关系数r=0.9919；所测定的虎杖饮片中白藜芦醇平均含量为0.304％，平均回收率为103.0％～90.4％；实验表明，以聚苯乙烯为基架的反相介质对于虎杖中自藜芦醇具有良好的分辨率，适合其有效成分的检测、分离，对虎杖中白藜芦醇制备的科学研究过渡到工艺开拓有很好的借鉴。

In our studies,sludges composting was used as object, fluoranthene was on behalf of PAHs,and methods included dynamic sampling,room analysis,sludges' composting,leaching of soil column,and room culture were employed. The main contents include:the concentration and the distribution of PAHs in municipal sludges in Jiangsu Province,effects of composting on the concentration of PAHs in municipal sludges,the physicochemical properties of water dissolved organic matters in sludges before and after composting,effects of sludges composting and DOM on the desorption-leaching and eco-toxicity of FLT in polluted soil,and primary investigation of mechanism on same phenomena.

本论文以污泥堆肥为原料，以荧蒽(fluoranthene，FLT)为PAH的代表，采用动态取样、室内分析、污泥堆肥、土柱淋滤和室内培养等方法，系统研究了江苏城市污泥中PAHs的含量以及分布模式；堆肥对污泥中PAHs含量的影响；污泥堆肥前后水溶性有机物的理化性质特征变化；污泥堆肥对污染土壤中FLT解吸-淋滤、以及生态毒性的影响，并对有关现象进行了机理探讨。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。