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Fig.1 SHEE cultured on coverslide, the living cells were growing in single layer with rich cytoplasm, the nuclei were uniform in size with a nucleolus ph ×400 Fig.2 SHEE had a nucleus with ellipse shape, large nucleolus and the cytoplasm contained mitochondria and tonofibrilEM ×10 000 Fig.3 SHEE was spherical in shape, with pseudopods attached on petri dish and abundant villi on cell surface SEM ×5 000 Fig.4 Same as in Fig.3, cell attached on petri dish, appeared stellate or polygonal in shape, with abundant pseudopods and cytoplasmic processes. Protrusive nuclear region in central part of the cell had more micro-villi SEM ×5 000 Fig.5 Chromosomes of SHEE cells belonged to diploidy type Giemsa ×1 000 Fig.6 The SHEE cells of stained in dark brown by Ki67 immunohistochemistry were the proliferative cells Immunohistochemistry ×400 Fig.7 In SHEE cell culture, the nucleus stained red or pink by PI was dead cell, the green nucleus was living cell Fluorescent ×400 Fig.8 The cell labeled by TdT was apoptotic cell in which the chromatin of nucleus condensed in block, a pyknotic nucleus in the upper right conner was seen TdT labeled ×400
图1 SHEE培养在盖坡片上,活细胞单层生长,胞浆较丰富,细胞核大小一致,有核仁×400 图2 SHEE培养细胞细胞核椭圆形,核仁较大,胞浆有较丰富的线粒体和张力原纤维EM ×10 000 图3 SHEE细胞呈球状,有伪足贴壁,表面有密集微绒毛SEM ×5 000 图4 同上细胞贴壁,呈星状或多角形,有丰富伪足和胞浆突,核区隆起有较多微绒毛SEM ×5 000 图5 SHEE细胞染色体仍属二倍体Giemsa染色×1 000 图6 SHEE细胞Ki67免疫组织化学染棕黄色为增殖细胞×400 图7 SHEE培养细胞出现死细胞,胞核和胞浆PI染色呈红色或淡红色,蓝色细胞核为活细胞荧光显微镜×400 图8 细胞TdT标记阳性为凋亡细胞,染色质凝集呈块状,右上角有一固缩细胞核TdT标记×400
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the title compound was synthesized and its structure was studied in theory.the schiff base compound was synthesized by condensation of paeonol with furylfurylamine,then it was deoxidized by sodium borohydried.the energies,bound lengths,bound angles,the net charges of main atom and contributive percents of frontier orbital in the title compound have been calculated by software hyperchem 7.0 in half experience method.the yield rate of title compound was 65.6%.the result of calculation showed that the most of the bond lengths and angles in the system were in the the normal range,negative charge mainly concentrate on o1,o2,o3 and n1.the title compound behaves as quadridentate ligand.
目的合成标题化合物并对其结构进行了理论研究。方法以丹皮酚和糠胺为原料合成席夫碱,经硼氢化钠还原得标题化合物;使用hyperchem7.0程序,用半经验方法计算了化合物分子中能量分布、主要键长、键角以及主要原子的净电荷分布和前言轨道分布。结果标题化合物,收率65.6%;计算结果分析表明,分子中主要原子之间的键长、键角基本在正常的范围内;负电荷主要集中于o1,o2,o3和n1上。
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The mean Harris score was 47.3 preoperatively and 83.2 postoperatively. The excellent and good rate was 90.1%. The position of prosthesis was appropriate, no loosening or sinking was found at the final follow-up evaluation. Radiotransparent region was found in one region of one case three months after surgery but with no sclerosis, rest pain or activity pain, fever, or local swelling.
Harris评分由置换前的平均47.3分上升到置换后的83.2分,优良率90.1%,最近一次随访X射线片评价假体在位良好,无松动、下沉现象,关节功能恢复情况满意。1例翻修患者于髋关节置换后3个月复查时发现1区有局限性透光带,无明显硬化带,患者无静息痛或活动疼痛,无全身发热、局部红肿表现,为骨长入性稳定。
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Results :Satisfactory facial appearance was achieved in all the patients . Results showed that the repeatability was 100% when error tolerance was 3.5 mm. If tolerance was set at 2 mm, the repeatability was 97.8%.The biggest difference occurred in ramous area.
结果:5例患者均获得满意的术后形态,在差值分析分析中,设允许误差为2mm,真实术后与虚拟手术的吻合程度为97.8%;设允许误差为3.5mm,两者吻合程度为100%;最大差别产生的区域在双侧升支。
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The project implemented a systemic investigation to the pupa period of wild silkworm comparatively, which from Shanxi, Jiangsu, Sichuan, Chongqing, Guizhou provinces and so on. According to the investigation, the pupa dormant characteristic of Shaanxi wild silkworm was found, and the individual, which pupa period was more than two months was selected. Through the hybrid genetic analysis we found that the characteristics for a long period of pupa was controlled by polygene and genetic recessively and strongly influenced by environmental conditions at the same time. To compare the performance of pupa period of the pupa dormant wild mulberry silkworm, we designed different ecological conditions. The result indicating that the temperature is the main factor in the ecological impact, but the pupa period was extended at the same temperature conditions, indicating that the additive effects of the environmental factors.
本项目实施,系统调查比较了陕西、江苏、四川、重庆、贵州等省市的野桑蚕的蛹期经过,发现了陕西野桑蚕的蛹休眠特性,筛选获得了长达2个月以上的蛹期经过个体;通过杂交遗传分析,发现长蛹期特性表现为隐性遗传,受多基因控制,同时受环境条件的强烈影响作用;通过设计不同生态条件比较蛹休眠性野桑蚕蛹期经过的表现,表明温度是主要的生态影响因子,但相同温度的野外型条件下蛹期经过延长,表明受到环境因素的综合作用。
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The total effective rate was 95.5%.There was 25 recrudescent patients,but the recrudescent symptom was all light,repeating the treatment was still obvious effect and efficacious.and there was no severe complication.
复发病例25例(22.3%),复发后症状均较轻,疗效仍为显效和有效,无严重并发症发生。
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Results EROD activities in liver of wild crucian from Zhangtan, Jiangjunyan, Fuyang and Wenyan (23.51±4.17, 16.79±7.39, 18.74±5.16, 18.65±8.86 nmol/) were significantly higher than that of Jiekou (7.84±2.42 nmol/g promin), and no difference was observed between the other sites and Jiekou. Three dose groups (0.25, 0.50, 1.00L/dish water sample extracts) were used in Ames test. Reversional mutation rate of TA98 colony exposed to extracts from Zhangtan was 2.21, 3.39, 6.95 without S9, and 2.70, 4.07, 6.23 with S9, respectively. The revesional mutation of TA100 colony was 2.55, 2.81, 5.36 without S9, and 1.73, 2.86, 4.14 with S9, respectively. Reversional mutation rate of TA98 colony exposed to 1.00L/dish extracts from Jiangjunyan with S9 was 2.22, and there was a dose-response relationship in reversional colony count. Ames tests of the other extracts were negative.
结果 樟潭、将军岩、富阳、闻堰点位野生鲫鱼肝EROD酶活性分别为(23.51±4.17),(16.79±7.39),(18.74±5.16),(18.65±8.86)nmol/,明显高于街口[(7.84±2.42)nmol/],其他点位与对照比较差异无统计学意义;樟潭水样浓集物在浓度为0.25,0.50,1.00 L/皿时在不加与加S9条件下,TA98回变菌落突变率MR值分别为2.21,3.39,6.95,2.70,4.07,6.23;TA100回变菌落MR值分别为2.55,2.81,5.36,1.73,2.86,4.14;Ames试验结果为明显阳性;将军岩水样浓集物在浓度为1.00 L/皿且加S9条件下TA98MR值为2.22,且在浓度为0.25,0.50,1.00 L/皿加S9条件下TA98回变菌落数呈剂量-反应关系,MR值分别为0.88,1.98,2.22;Ames试验结果为弱阳性;其他点位均为阴性。
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Finally, simulation software was developed with Visual C++ and Delphi for 2D molding analysis of MIM, which affords facilities for the quality forecast of 2D thin wall parts and optimal design of mold and process parameters. In the research of molding performance of MIM feedstock, for the shortcoming of capillary rheometry, it was propounded that using spiral mold test molding performance of feedstock, and a set of spiral mold was creatively designed with quickly exchange gates and closed mold cavity. With the spiral mold, an orthogonal experiment was made on getting regress equation of flow length L〓 of the heavy alloy feedstock. Then the rule of L〓 varying with Q〓, P〓, and ΔT〓 was discovered. Q〓 ranks first in the influence on process parameters of molding performance, P〓 and Δ T〓 second. Therefore the injection rate Q〓 should be controlled primarily to improve the flow performance of feedstock.
在MIM喂料注射成形性能研究方面,针对毛细管流变仪测试法衡量流动性能的不足,提出使用螺旋线模具测试喂料熔体的注射成形性能,创造性地设计了一套可快换浇口,模腔封闭的螺旋测试模,并在螺旋测试模上采用正交实验设计,得出了高密度重合金喂料流动长度L〓的回归公式,揭示了L〓随Q〓、P〓、△T〓和S〓的变化规律,并得出工艺参数对成形性能影响的强度顺序为Q〓最大,P〓与△T〓其次,因而改善喂料流动性能应优先控制注射速率Q〓。
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Our results showed that the most appropriate rib's level for applying force was below the apex of scoliotic spine. From this appropriate level, the stress delivered to its conjunctive vertebra was the highest, as 1.3~1.8 times compared with others. Furthermore, the correction of Cobb's angle was also the highest, achieved 15%. The ML force was dominant in alteration of Cobb's angle, whereas the AP force was dominant in alteration of axial vertebral rotation. In addition, initial vertebral rotation influenced neither correction nor stress.
研究结果显示由凸点椎体的下一节对应之肋骨施力能使传入椎体具最高平均应力,较其他施力位置高出1.3到1.8倍之间,对柯卜氏角度可达15%的最佳矫正率;其中侧向施力主导冠状面上的矫正,而前后向施力主导扭转角度的矫正;另外椎体初始旋转角度对侧弯矫正和应力分布均无明显影响。
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In Wistar rats, latent period of a-wave in scotopic rod response after 1-week transplantation was shorten (P.05); and latent period of a-wave in scotopic rod response after 2-week transplantation was prolonged (P.05); while latent period of b-wave in scotopic maximum response after 1-week transplantation was shorten (P.05). In RCS rats, amplitude of a-wave in scotopic rod response and photopic response after 4-week transplantation was increased (PO.05); while latent period of a-wave in scotopic rod response after 4-week transplantation was shorten (PO.05).Conclusion 1. At the age of 3 months, photoreceptors of the RCS rat degenerated completely, which leaded to the reduction of amplitudes of a-wave and b-wave.
Wstar大鼠术后1周的暗适应视杆反应,a波潜伏期缩短o叼。05);术后2周的暗适应视杆反应,a波潜伏期延长o功刀5):术后1周的暗适应最大反应,b波潜伏期缩短o功刀5卜*CS大鼠术后4周的暗适应视杆反应和明适应反应,a波振幅增大o功刀匀;术后4周的暗适应视杆反应,a波潜伏期缩短o<0卜结论1、RCS大鼠在3月龄时感光细胞变性死亡,导致FERG的a、b波振幅明显降低;视网膜下腔仍存在感光细胞外节盘膜碎屑,外丛状层缺乏感光细胞的突触末端,同时Muler细胞出现反应性胶质纤维增生。
- 相关中文对照歌词
- When Everything Was What It Was
- Sun Was High (So Was I)
- Jesus Was Our Saviour And Cotton Was Our King
- Before There Was Atlanta, There Was Douglasville
- Phil Cosby (Before There Was Atlanta, There Was Douglasville)
- I Didn't Say I Was Powerful, I Said I Was A Wizard
- All I Was Looking For Was You
- Hò Bha Mi, Hé Bha Mi (Hò I Was, Hè I Was)
- The Moon Was Yellow (And The Night Was Young)
- Stella Was A Diver And She Was Always Down
- 推荐网络例句
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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.
在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。
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In fact,I've got him on the satellite mobile right now.
实际上 我们已接通卫星可视电话了
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The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.
世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。