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During the early growth stage of seedlings, basal stem diameter and paripinnate number were decreased with the increasing of shade, taproot length, root : shoot ratio, total dry weight and leaf area per seedling were maximal under 37.3% NS, specific leaf area was increased with the increasing of shade, but the relative growth rate was declined. Seedlings grew fastest under 0.6% NS, suggesting that nutrients stored in seeds played an important role at the early growth stage. The treatment of 37.3% NS was best for established seedlings in seedling height, basal stem diameter, paripinnate number, rachis length, the maximum sub-leaflet number of individual paripinnate, leaf area per seedling, relative growth rate and net assimilation rate. Light intensity was positively correlated to total dry weight, and negatively to specific leaf area. The maximum of SLA was observed under 15.5% NS.

生长早期的幼苗基径和复叶数随遮荫程度的增加而降低；主根长、根冠比、总干重和单株叶面积均以37.3% NS处理最大；比叶面积随遮荫程度的增加而增大，而相对生长率则降低；幼苗株高在0.6% NS下增长最快，表明种子中贮藏的营养物质对幼苗的早期生长可能具有重要作用。37.3% NS处理对定居后绒毛番龙眼幼苗的生长最有利，幼苗的株高、基径、复叶数、叶轴长、复叶最多小叶数、单株叶面积、相对生长率和净同化率均在37.3% NS处理下获得最大增长；幼苗总干重随光照强度的减弱而降低；比叶面积在15.5% NS处理时最大。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

In addition, a pre-column derivatization HPLC method was applied to the determination of Astragaloside Ⅳ; the flavonoids was determined by full-range HPLC scanning; a colorimetric method was developed for the determination of flavonoids and saponins. Hierarchical cluster analysis was performed on three constituents of different Radix Astragali species, and its result was compared with those of traditional phytoecological identification and the pharmacological quality assessment.

本研究以比色法测定了黄芪中总皂苷的含量，以柱前衍生化高效液相色谱法测定了黄芪中黄芪甲苷的含量；以比色法测定了黄芪中总黄酮成分的含量，以反相高效液相色谱法测定了黄芪中毛蕊异黄酮和毛蕊异黄酮苷的含量；以黄芪中的黄芪甲苷、毛蕊异黄酮和毛蕊异黄酮苷为主要特征对不同品种的黄芪进行了系统聚类分析，并将聚类结果与传统生药学鉴定和药理质量评价结果进行了比较。

Han dynasty's pinacle of development was in Fu, Tang was in Shi, Song was in Ci, Yuan was in Qu (melody/opera), Ming/Qing was in novel.

汉代pinacle发展是福，唐在石，宋是在次，袁是在曲，明/清是小说。

Our work reported here are aiming at improving nitrogen and water use efficiency in wheat/maize, faba bean/maize, pea/maize and soybean/maize intercropping through field experiment.The main results obtained were as the following:(1) Land equivalent ratio was greater than 1.0 for faba bean (Viciafaba L.)/maize (Zea mays L.) intercropping regardless of nitrogen fertilizer application or not. Based on grain yields production, LER of pea (Pisum sativum L.)/maize intercropping was over 1.0 in spite of nitrogen fertilizer application, while LER was higher than 1.0 only when nitrogen fertilizer was applied based on biomass production. However, LER in soybean (Glycine max L.)/maize intercropping was smaller than 1.0 both nitrogen applicationa and no nitrogen application. In wheat Triticum aestivum L.

本文通过田间试验，对小麦／玉米、蚕豆／玉米、大豆／玉米和豌豆／玉米间作种植下作物对氮素和水分利用的影响进行了系统比较，获得的主要结果如下：(1)蚕豆／玉米间作的土地当量比都大于1；豌豆／玉米间作以籽粒产量为基础的LER无论施氮还是不施氮都大于1，而以生物学产量为基础的LER只有施用氮肥时才大于1；大豆／玉米间作无论施氮还是不施氮LER均小于1。

Because of nitrogen, the value of IAA/ABA、GA3/ABA、(IAA+GA3+ZR)/ABA in grain promoted at seed filling stage and at the mature stage.6、The adjustment of nitrogen levels on carbon and nitrogen metabolism.Nitrogen reduced the C/N in leaves and stem. When the nitrogen rate was 50 kg/hm2, the effect on stem was obvious. When the nitrogen rate was 100 kg/hm2, the effect on leaves was obvious. The C/N in roots lower before seed filling stage and the effect was not obvious after seed filling stage.7、Effect of nitrogen levels on yield traitsPlant height, pods per plant and full grain number were increased and bottom-pod height and podded node were decreased by applying nitrogen.

施氮在鼓粒期、成熟期均提高了子粒中IAA/ABA、GA3/ABA、(IAA+GA3+ZR)/ABA的值。6、氮素水平对大豆碳氮代谢的调节施氮降低了叶片和茎秆中C/N的比值，施氮量为50 kg/hm2时，对茎秆作用效果明显，施氮量为100 kg/hm2时，对叶片效果明显；施氮降低了根系鼓粒期前的C/N比值，而鼓粒期后作用效果不明显。7、氮素水平对产量性状的影响施氮增加了大豆的株高、株荚数、饱满粒数，降低大豆的结荚高度、结荚结位。

The time of the pedogenic process was in the last ice age, therefore this area was colder and drier in climate and lower in sea level than today. The main chemical process in the soil was oxidation, a period of the formation of oxide minerals such as limonite. With the deposition and covering of the marine facies layer in the postglacial period, the soil turned into diagenetic process. Consequently the chemical process in the soil was dominated by reduction and this process was the main development period for nodules of iron-manganese carbonate to form.

成土期处于末次冰期低海面、气候寒冷干燥期，土壤的化学过程是以氧化作用为主，是褐铁矿等氧化性矿物形成时期；冰后期海相层的沉积覆盖使土壤层进入成者阶段，土壤层的化学过程以还原作用为主，成岩过程是铁锰碳酸盆结核的主要生成期。

The average removal efficiency of TP and COD was 28.76% and 8% after cloth media filter without coagulation, and TP removal was influenced by the influent water quality. However, precoagulation with coagulants of ferric chloride, PAFC, PAC, the average removal efficiency of TP was 63.58%, 60.13% and 66.94% respectively, and no influence of TP removal can be found by the influent water quality. The average removal efficiency of COD was of 17.7%,26.3% and 27.7%,SS was 70.7%,64.3% and 49.1%.

当不投加混凝剂时，滤布滤池对TP和COD的平均去除率为28.76%和8%，其中TP的去除效果受进水水质影响较大；当投加氯化铁、聚合氯化铝铁与聚合氯化铝强化滤布滤池时，滤布滤池对TP的平均去除率分别为63.58%、60.13%和66.94%，TP去除效果受进水水质影响较小；对SS的平均去除率分别为70.7%、64.3%和49.1%；对COD平均去除率分别为17.7%、26.3%和27.7%。

The results indicated that ultra-low nitrogen (0.001 0%-0.008 0%, w/%) in low alloy steel could be determined accurately under such optimized analytical conditions as follow: the addition of deoxidizing agent was less than 0.35%, multi-head milling cutter should be chosen and the milling depth was 1.4 mm while sampling, the mass percentage of argon was no less than 99.999 5%, the washing time was set as 8 s, the precombustion time was set as 3. 3 s and energetic precombustion energetic measure optical source mode is adopted.

结果表明，取样时控制脱氧剂加入量不超过0.35%且选用多刀头铣刀盘，铣样深度为1.4 mm；在氩气的质量分数不小于99.999 5%，冲洗时间为8 s，预燃时间为3.3 s，采用高能预燃高能测量光源方式等优化的分析条件下，可有效测定低合金成品钢中质量分数为0.001 0%~0.008 0%的超低氮。

The shredding of agro-residues, such as corn stalk, straw, wood sawdust and other herbaceous biomass, were investigated in this dissertation. According to the machinery mechanics of shredding, the components, shearing strength and pressive strength of the biomass were analysed in theory, which played a critical role during the shredding process. And the powder was examined by Scanning Electron Microscopy. It was founded that shearing,splitting and grinding force were appropriate for shredding biomass. The results also showed that primary shredding was the bulk shredding model, which was mainly affected by shearing and splitting force, therefore the curly knife and the straight knife were useful; while the fine shredding was the surface shredding model, affected by splitting and grinding force, and the straight knife useful.

本文以农林废弃生物质为研究对象，结合机械力破碎基础理论，从影响其破碎的化学组分和剪切强度、抗压强度等力学角度进行了理论研究，并运用扫描显微镜对破碎产物的微观结构进行了实际分析，结合二者得出如下结论：最适合生物质的施力方式为剪切力、劈碎力和磨削力；生物质粗破碎为体积破碎，主要受剪切力和劈碎力作用，刀片宜设计成弯口刀和单面直刃刀；细破碎为表面破碎，主要受剪切力和磨削力作用，刀片宜设计成双面直刃刀。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。