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The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

But you already had your subordinate,but I, only was lonelily go facing all these, why has to injure mesuch deeply, all mistakes all is I, if is not I has no scruples, couldnot cause the today such tragedy, I thought, happy was, but I whichhad to depend on oneself strives for mistakenly, have been happy amtwo people together strive for, but you actually were wholeheartedlythe dual purpose, therefore we have missed happiness, has brewed theresult which could not be recalled In my birthday that day, you have sent the short note to me, reallylets me very happily, I affected have cried, although also is alone aperson, but the feeling is happy, but no matter how, we all couldn'treturn to the past, although this I all could regret for a lifetime, Iactually helpless, only hoped you could be happy Although we did not have the opportunity, but in my heart, I only hada love, I was a single-minded person, I did not have the means to letoneself love second individual again, I could not achieve, you were mythis life only love, only let the person which I paid, but I haveactually been defeated, I all was unable to forgive for a lifetimeoneself, I had said I could always wait for you, was alwayswaiting......

可是你已经有了你的所属，而我，只是孤单地去面对这一切，为什么要伤害我这么深，所有的错误都是我，如果不是我毫无顾忌，就不会酿成今天这样的悲剧了，我以为，幸福是要靠自己去争取的，但是我错了，幸福是两个人共同争取的，而你却是一心两用，所以我们错过了幸福，酿造了不可挽回的结局。在我过生日的那天，你发短信给我，真的让我很高兴，我感动地哭了，虽然又是独自一个人，但是感觉是幸福的，但是不管怎样，我们都回不到从前了，虽然这一辈子我都会遗憾，我却无能为力了，只希望你会幸福。虽然我们没有机会了，但是在我心里，我只有一份爱，我是一个专一的人，我没有办法让自己再去爱第二个人，我做不到的，你是我这一生唯一的爱，唯一让我付出的人，可我却失败了，一辈子我都无法原谅自己，我说过的我会一直等着你，一直等着。。。。。。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

When lipase N435 was 2.5gL^(-1), RS-ethyl mandelate was 0.25gL^(-1), water to RS-ethyl mandelate was 5:1, the reaction temperature was 40℃, the speed of agitation was 200rmin^(-1), the R-ethyl mandelate conversion could reach 41.6%, and enantiomeric excess e.

确定了最适的反应条件：脂肪酶N435浓度为2.5gL^(-1)，RS-扁桃酸乙酯浓度为0.25molL^(-1)，水：RS-扁桃酸乙酯的摩尔比为5：1，反应温度为40℃，摇床转速为200rmin^(-1)，反应时间为24h。

Methods The experimental mouse was injected with high, middle, and low dosages of marasmius androsaceus po1ysaccharide 5, 10, 20 mg kg^(-1d^(-1) in the abdomen, and the comparison group was given the same volume of saline water i. p. once a day. After treatment for 14 consecutive days, the blood cell count instrument was used to measure the mice hemogram, the phagocytic function of peritoneal macrophage and the ability of carbon granular clearance were observed., the ability of the spleen lymphocytes proliferation was tested by MTT, and the hemolysin production test was used to evaluate the influence of MA polysaccharide on specific humoral immunity in mice.

小鼠腹腔注射不同剂量的鬼毛针多糖5， 10， 20 mgkg^(-1d^(-1)，对照组给予等量生理盐水，每天给药1次，连续14d后，用血细胞计数仪检测小鼠血象；用腹腔巨噬细胞吞噬鸡红细胞实验、碳粒廓清实验观察小鼠吞噬细胞的吞噬能力；采用MTT法测定小鼠脾T淋巴细胞增殖能力；采用血清溶血素形成实验观察鬼毛针多糖对小鼠特异性体液免疫功能的影响。

And the scientific research showed that they are medicable to insomnia, anxiety and other diseases that found in modern people. 4 The regression models of biomass were used to calculate the total cumulative biomass. Average biomass of six samples was 62.88 t·hm-2 after cutting 3 years, which was higher than that of tropical secondary forests with 5 years succession. It can be concluded that traditional fuel woods had played an important role in conservation of tropical forest in the area. Caloric values were not different in major fuel species (Coefficient of variation was only 1.1%). The similar result was found in stems of C. siamea after growing different years. The main reason of C. siamea cultivation was that it had the character of hardwood, easy collecting, little ash, and so on.

铁刀木的各个部位都作药用，资源量大的树叶和心材使用最多，并且现有的研究成果表明它们具有多种活性成分，针对现代人常患的失眠、焦虑等疾病具有较好疗效。4）通过收获法拟合的生物量回归模型对推测西双版纳地区铁刀木林薪柴蓄积量有较高的参考价值，砍伐3年后的六个样方中铁刀木的平均生物量为62.878 t.hm-2，高于5年林龄的热带次生林（16.35t·hm-2），对西双版纳热带雨林的保护有着重要意义。5）几个村寨最主要的薪材树种热值研究结果变异系数仅为1.1％，各薪材树种之间热值相差不大，铁刀木被选择为传统薪炭林更多是因为材质坚硬、收获运输方便、燃烧完全等特点。

The treatment of car-washing wastewater by using membrane bioreactor was investigated. The influences of operating pressure and environmental factors on the membrane's filtration function were analyzed combining with theoretical calculations. In the meiobar range, membrane resistance was the dominant role, independent of operating terms. In the middle pressure range, concentration difference-polarized resistance was the dominant role, depending much on operating terms. In the high pressure range, gelatin resistance was the dominant role. The influences of the operating pressure was small on the membrane flux, and the membrane's separating characteristics were related much with mud concentration, membrane flow velocity and etc.

通过膜生物反应器处理某车辆厂洗车废水的实验研究，结合理论计算，分析了操作压力及环境因素对膜过滤性能的影响研究结果表明，在低压区，膜本身阻力占主导地位，它与操作条件无关；在中压区，浓差极化阻力占主导地位，它与其他操作条件关系密切；在高压区，凝胶层阻力占主导地位，操作压力增加对膜通量影响不大，在此阶段膜分离特性还与污泥浓度、膜面流速等操作条件有关。

He type of TGF β1 expression was related with lymph node metastasis and prognosis of patients. The positivity rate of TGF β1 expression only in mesenchyma in the group on lymph node metastasis was significantly lower than that in no lymph node metastasis, but the positivity rate of TGF β1 expression only in cytoplasma was higher. The prognosis of TGF β1 expression only in mesenchyma was better, but the prognosis of TGF β1 expression only in cytoplasma or both mesenchyma and cytoplasma was poor.

GF β1的阳性表达类型与肺癌的淋巴结转移及预后有关，淋巴结转移阳性组TGF β1的单一间质阳性表达率显著低于淋巴结阴性组，而单一胞浆阳性表达率高于淋巴结阴性组；半年内死亡组的TGF β1单一间质阳性表达率低于5年以上生存组，而间质胞浆均阳性的表达率及单一胞浆阳性表达率则高于5年以上生存组(P均＜0.05)。

The results showed that the structure of St-g-PAM was meshy,and porous,and integrant linetype,the soluble starch had elliptic form,and PAM was linear color;the crystallinity of St-g-Pam had been improved,and was better than PAM;the thermal stability of St-g-PAM was also better than PAM,which was the significant reason that the graft polymer had the ability of excellent flocculation.

线型St-g-PAM作为一种新型的高分子絮凝剂，它结合了淀粉与聚丙烯酰胺的特性，广泛应用于废水处理、石油化工、造纸工业等领域[3~5]。由于其原料淀粉来源丰富、价格低廉，且线型St-g-PAM的成本比PAM低，故越来越受到人们的重视。

And the conditions in cell suspension culture of Mikania micrantha were studied. The results showed that sucrose was the compatible carbon sucrose, and 30g/L sucrose concentration can satisfy the growth of Mikania micrantha cell; ammonium was absorbed under different sucrose concentration that haven't demonstrated significant specificity, and was completely absorbed on the lag phase and the early logarithmic phase; while nitrate was mainly absorbed on logarithmic phase. The density-dependent of Mikania micrantha cell starting to grow and density-inhibited of cell growth were proposed, the fittest inoculating quantity of Mikania micrantha in cell suspension culture was 40g/L.

并对微甘菊细胞悬浮培养条件进行研究，结果表明，微甘菊细胞生长适宜的碳源是蔗糖，并且较合适的蔗糖浓度为30g／L；在不同蔗糖浓度下氨基氮的吸收差别不大，氨基氮在迟滞期和对数期前期已基本消耗完毕，对数期生长主要利用硝基氮，因而提出，在微甘菊细胞液体悬浮培养中，氮源可以采用初始低氨基氮浓度，对数期中间维持高硝基氮，而且对数期中逐步流加少量的氨基氮；微甘菊细胞迟滞期分裂启动存在密度依赖现象，对数期生长存在密度抑制现象，最适接种量为40g／L。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了