查询词典 was

ResultThe maximumabsorption wavelength of reaction solution was about 720nm.The fermentation volume,adding the activated carbonor not,pHvalue of reaction solution and so on had no great effect onthe determinationresults and they could increase the screening efficiency.The effect of ultrasonic treatment for20min was obvious.When50ml volumetric flask was used,the suitable sampling volume was 2.5ml.It was better to centrifugate the fermentationliquid and the supernatant could be taken after standing inthe determination when necessary.

结果反应液最大吸收波长在720nm左右；发酵体积、加入活性碳与否、反应液的pH值等对测定结果影响不大，在筛选时可大大提高效率；超声波处理20min效果明显；在用50ml容量瓶进行定容时，取样体积以2.5ml为宜；对发酵液进行离心效果更好些，但在必要的情况下，可静置后取上清测定。

Regarding the thermobaric explosive further tests, the research showed that the compressive stress was 6.699MPa; The tensile stress was 0.716MPa; Heat sensitivity was 288℃(5s); The anti-overload capacity may withstand 1.0×104g launch overloads and smaller than 2×105g the impact deceleration overload; first decomposition temperature was 251.9 ℃, highest exothermic peak temperature was 286.9 ℃; In the compatibility, except the ceresin and Oxidant, the ceresin and the F rubber, the ceresin and Al compatible rank could reach to second level of compatibility, the others could reach to the first level of compatibility.

对此粘结-钝感体系包覆的温压炸药样品进一步测试研究显示：抗压强度为6.699MPa；抗拉强度为0.716 MPa；热感度为288℃（5s爆发点）；抗过载能力为可承受1.0×104g的发射过载和小于2×105g的撞击减速过载；安定性为分解温度251.9℃，最高放热峰温度286.9℃；内相容性为除蜡类钝感剂+氧化剂，蜡类钝感剂+F橡胶，蜡类钝感剂+Al相容性等级为2级相容性较好外，其他组分间相容性均为1级，相容性很好。

There existed dual-critical effect of such organic retarders as sucrose and citrin on cement hydration. First, when the amount was relatively low, cement hydration was delayed. While the amount was comparatively high, initial hydration of cement was accelerated, but paste was not hardened after a long time. Second, there existed a critical amount.

蔗糖、柠檬酸对水泥水化历程的调控作用存在双临界效应：一、在掺量较低时，表现为缓凝效果，当掺量较大时，则表现为促凝作用，但是浆体长时间不硬化；二、缓凝效果存在临界掺量值，低于临界值时，缓凝效果随掺量增加而增加，超过此临界值时，缓凝效果随掺量增加而下降。

Methods The compound preparation was administered intragastrically in mice, whose spontaneous activity was observed along with their tolerance of the preparation after long-term administration. The hypnotic effect of the compound was assessed by investigating the changes in the pentobarbital sodium-induced sleeping. The compound was tested for its antiseizure effect in mice with pentetrazole-induced clonic and tonic convulsion. Diazepam was used as the standard control in all experiments.

利用光电计数法观察灌胃给予复方酸枣仁油栀子油对小鼠自发活动的影响，以及长期使用药物对小鼠耐受性的影响；利用延长戊巴比妥钠睡眠时间实验和戊巴比妥钠阈下催眠剂量实验，观察复方酸枣仁油栀子油的催眠作用；利用戊四氮诱发小鼠阵挛性、强直性惊厥观察复方酸枣仁油栀子油的抗惊厥作用；本实验用地西泮进行平行对照实验，并进行了组方设计和实验。

Then the combinant plasmid was conducted into ACC-2 cell by electroporation. ACC-2 cells stably expressing CD was obtained by 10-day positive selection with 400 μg/mL G418. Total RNA was extracted and the expression of the CD gene in transfected ACC-2 cells was identified by RT-PCR. RESULTS: PCR yielded a fragment of l280bp and CD was verified by sequence analysis.

测序正确后，将其亚克隆到质粒表达载体pIRES中，构建以内部核糖体进入位点相连的CD基因的质粒表达载体pIRES-CD，采用电穿孔法，以质粒表达载体转染ACC-2细胞，用400μg/mL的G418筛选10d，获得稳定表达CD基因的ACC-2细胞系，提取该细胞的总RNA，用RT-PCR检测CD基因的表达。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Hosius drew out the conciliar statements, to which anathemas were subjoined against those who should affirm that the Son once did not exist, or that before He was begotten He was not, or that He was made out of nothing, or that He was of a different substance or essence from the Father, or was created or changeable.

侯休斯拔出了conciliar报表，其中anathemas被subjoined对那些应该肯定地说，儿子一旦不存在，或者说，以前他是造物主，他不会，或者说，他是出于什么，或者说，他是一个不同物质或实质上来自父亲，还是创造或复杂多变。

The atrium was raised to o higher level. The fa?de retreated back about two metres. The Constantinian mosaic floor was covered up with two feet of imported soil and a new marble pavement was laid at a higher level. The lower part of the interior walls was also covered with marble and the upper parts with mosaics. It was one of these mosaic scenes that spared the Church from destruction during the Persian invasion (614 AD).

中庭被提高了，正面退缩了约两米， Constantinian 马赛克地板被覆上两英尺的泥土之后铺上大理石，殿内墙壁下层亦覆以大理石，上层是马赛克，其中一幅马赛克使教堂在波斯人入侵（西元614年）时免於被摧毁。

Methods One normal adult male was choosed, and his left elbow joint performed in pronation and extention position was scanned by spinal CT, and the two-dimensional image was dealed with Mimics 1 0 software, the three-dimensional finite element model was built up by Abaqus 6.7 software. Finite element calculation was performed to simulate the impact of 1 000 N on the elbow joint, and the stress distribution in the radial head and coronoid were analyzed.

选取1名正常成年男性为研究对象，使其左肘关节处于旋前伸直位，行螺旋CT断层扫描，得到CT数据二维图像，采用Mimics 10.0软件系统处理，利用Abaqus 6.7建立肘关节三维有限元模型，模拟施加1000 N冲击载荷，分析冠状突及桡骨小头内应力分布情况。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。