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Under the optimum conditions, the linear range of the method was 0-80 μg/L. The detection limit was 4.58×10^(-7) g /L.The catalytic reaction was the zeroth order one. The apparent activation energy and the reaction rate constant was found to be 96.04 kJ/mol and 3.61×10^(-3)/s, respectively. The sensitivity was increased by 3 times in the presence of surfactant.

在最佳实验条件下,方法线性范围为0~80μg/L,检出限为4.58×10^(-7)g/L;催化反应为动力学零级反应,表观活化能为96.04kJ/mol,反应速率常数为3.61×10^(-3)/s;反应在表面活性剂存在下,灵敏度提高3倍。

Results The score of openness of sexual thought was 3.38±0.71, diversity of sexual behavior was 2.83±1.06, acceptant of sex trade was 2.16±0.61, alterative of sex objects was 1.89±0.65 and abundance of sexual knowledge was 3.11±0.75; Male's scores were ...

结果被试总体性观念各因子的得分在1.89±0.65~3.38±0.71之间,即性思想开放性、性行为多样性、性交易接受性、性对象可选性和性知识丰富性;上述因子的得分,男生显著高于女生;四年级显著高于一、二、三年级;城市生源学生显著高于农村生源学生;性观念因子高分组与低分组的被试在心理健康因子上的敌对和偏执上差异显著,前者得分高于后者。

The observation of SEM, EDAX and IR analysis showed that the conformation of MPPE was changed, the surface and section of MPPE were rough and accidented, the specific surface area of MPPE was increased, and magnetic powders, HAP and other additives were separated in polyethylene polymer. The removal rates of Phenol by using PPE and MPPE were 82%, 93%, the removal rates of TOC could reach 78%, 89% respectively, when the phenol wastewater at 120mg/L concentration was treated with aerated process for 14 hours. The removal rates of Phenol were 85%, 90%, the removal rates of TOC reached 80%, 86% respectively when the phenol wastewater at 350mg/L was treated for 35 hours. The weight of biofilm formation on MPPE was 64% and 34% higher than that on PPE at this two wastewaters.

质量浓度为120mg/L的含苯酚废水在好氧生物填料柱中处理14 h后,PPE和MPPE对苯酚去除率分别为82%和93%,TOC去除率分别为78%和89%;质量浓度为350mg/L的含苯酚废水处理35h后苯酚去除率分别为85%和90%,TOC去除率分别为80%和86%;两种不同质量浓度的含苯酚废水降解后MPPE上微生物挂膜量分别比PPE提高约64%和34%。

The activity of superoxide dismutase in the myocardial cells was determined by the diphenol method, plasma malondialdehyde content was measured by TBA assay, the culture medium LDH content was measured by the acetonic acid method, the intracellular calcium concentration was measured by fluorescence spectropho- tometer- The results was shown that aspartate-K.

结果显示,门冬氨酸钾镁能减少阿霉素中毒心肌细胞乳酸脱氢酶的释放量,降低细胞内丙二醛含量及游离钙浓度,同时可升高细胞内超氧化物歧化酶活力;结果表明,门冬氨酸钾镁可能通过抗脂质过氧化及逆转细胞内钙离子过负荷作用进而保护中毒心肌细胞。

The first one is that the diene structure in Adda side chain was attacked and cleaved, which was named as Adda degradation pathway. The second one is that the pathway of Mdha and Ala degradation, which was that the peptide bond between Mdha and Ala was attacked and cleaved. Adda degradation pathway was dominant and mainly responsible for the degradation of MC-LR by ozone.

臭氧氧化的Adda途径是通过对MC-LR上Adda侧链的进攻,断开具有活性的Adda支链,而达到脱毒的目的;臭氧氧化的Mdha途径是通过对MCs肽环上面Mdha和Ala的断键,打开环状肽链,使藻毒素失去活性,在整个过程中Adda途径占主导地位。

The cultural condition of PGPR strains Temperature,pH,light,cultural method,acid or alkali producing,and salt tolerance of 9 PGPR strains isolated from Gramineous Forage were tested,the results showed that all the PGPR strains could grow in the temperature range from 5℃~45℃,and optimum temperature for 178,O-6,Dry6 strains was 35℃,for X5,173,Y5,C-6 strains was 30℃,for N4 strain was 25℃~35℃,for 86 strain was 25℃;most of PGPR strains prefered to neutral or alkaline condition,strains 178,O-6,N4 and X5 were preferable to alkali condition especially;light was beneficial to PGPR's growth;all of them produce alkali;most of PGPR strains were not sensitive to NaCl concentration;all the strains were aerobiotic bacteria.

结果表明:各供试菌株对温度的适应范围较广,在5℃~45℃范围内均能生长,178、O-6、Dry6 菌株的适宜生长温度为35℃,X5、173、Y5、C-6 菌株的适宜生长温度为30℃,N4 菌株的适宜生长温度为25℃~35℃,86 菌株的适宜生长温度为25℃;大部分菌株在中性或偏碱性的条件下生长好,特别是Dry6、N4 和X5 菌株对碱性环境适应性强,在pH 值8.0 时生长最好;光照有利于菌株的生长;绝大部分菌株在3%NaCl浓度下生长良好,在5%~7% NaCl 浓度下除173 和86 菌株外,其它菌株都能生长,即对盐份的耐受性较好,且9 个菌株均为产碱菌;除173 菌株是兼性厌氧细菌外,其它都是好氧性细菌。

For the bud directly differentiation , the suitable culture medium was MS+BA 2mg/L +NAA 0.2mg/L or MS+TDZ0.01mg/L +NAA0.2 mg/L, of which the induction rate was 88% and 100% respectively. An efficient tissue culture system has been developed with the bud of mature seed of Aesculus hippocastanum L. as explants . Buds were induced from 2cm high young plantlet cultured on MS medium supplemented with 0.6 mg/L 6-BA plus 0.1mg/L or 0.4~0.6mg/L ZT plus 0.1 mg/L NAA for 15 d, and the induction rate was 100%, the mean No. of buds was 35.7; The combination of MS+0.2mg/L 6-BA +0.1mg/L NAA + 10mg/L AD was the suitable culture medium for elongation of the buds.

以欧洲七叶树成熟种子的胚芽进行离体培养和快速繁殖,结果表明:高约2cm的无菌苗在MS+0.6mg/L 6-BA+0.1 mg/L NAA或MS+0.4~0.6mg/L ZT+0.1 mg/L NAA培养基上培养15 d左右可诱导出不定芽,分化频率为100%,平均每株产芽35.7个;MS+0.2mg/L 6-BA+0.1mg/L NAA+10mg/L AD培养基有利于芽伸长;生根培养基为1/2MS+0.4 mg/L NAA+0.2mg/L IBA时,生根率可达75%。

The study was aided by the Ailuropoda Fund and designed on account of the Chengdu zoo' s topic that the Detection of the feline distemper' s serum antibody and the foundation of its immune programme about the grow wild feline animal such as Panthera pardus L, et al. The study regarded the attenuated feline panleukopenia virus as the diagnostic antigen which was isolated from the tri-combinant vaccine of Feline rhinotracheitis virus, calicivirus and panleukopenia virus . According to the tesults of Virus Physical and Chemical Character Detection, Agar Diffusion Reaction and Virion Shape Electron Microscope Observation, it was affirmed that the virion which was isolated from the tri-combinant vaccine was feline panleukopenia virus .

本研究从猫瘟热病毒、猫鼻气管炎病毒、猫鼻结膜炎病毒三联弱毒活疫苗中分离猫瘟热病毒弱毒株,通过对分离株进行病毒理化特性检测、双向琼脂扩散试验检测和病毒粒子形态电镜观察,最终确认从三联弱毒疫苗中分离到猫瘟热病毒,并以此分离株作为诊断抗原。

Result] The color and elasticity of steamed breads made from 250 g flour added with 50 g cactus juice and 30 g amomum juice were bad and their surface was not smooth;when the addition amount of cactus juice was 70 g,the elastic extensibility of steamed breads was bad;when the addition amount of cactus juice was 90 g,the elasticity of steamed breads was good and their surfaces were smooth.

结果] 在250 g面粉中加入50 g仙人掌汁、30 g砂仁汁蒸制的馒头色泽、弹性不佳,表面不光滑;加入70 g仙人掌汁时馒头的弹性延伸性不好;加入90 g仙人掌汁时,馒头的弹性佳,表面光滑。

In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体,以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝,再将纤维溶於六氟异丙醇溶剂中配制成溶液,以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化,再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后,以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构,最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理,并分析其二级结构之变化。

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