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Imaging studies showed that the positive rate of the ultrasound was 73.33%,the CT was 60.00%,the MRI was 75.00%,and the 99mTc-MIBI was 100.00%.All the patients were underwent parathyroidectomy of front neck incision, and were identified with parathyroid adenoma in 16 cases, hyperplasia in 1case and carcinoma in 2 cases. Follow-up was done from 10 months to 6 years after surgery.

影像学检查的阳性率：B超为73.33％，CT为60.00％，MRI为75.00％，99mTc-MIBI为100.00％。19例患者均经颈前弧形切口行甲状旁腺切除术，16例为甲状旁腺腺瘤；2例为甲状旁腺腺瘤伴灶性癌变；1例为甲状旁腺增生。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Methods The cognitive impairment rat model was induced by hypodermic D-galactose injection and half fat feeder (6 weeks), the Morris water maze was used to screen the model rats, which were divided randomly into 4 groups (12 rats in each group): the model group, the hydrochloricdonepezil group (0.4 mg/kg, weight, sic passim), the low-dose Huannao Yicong Fang group (7 g crude drug/kg), the high-dose Huannao Yicong Fang group (14 g crude drug/kg), they were treated respectively by intragastric administration. Besides, a control group of 12 rats was set up, they were given tales doses of distilled water. After 4 weeks, the behavior of the rats was detected, the contents of plasma P-selectin and PAI-1 were measured by ELISA, also the hemorrheology was measured.

采用皮下注射D-半乳糖及喂饲半高脂饲料（6周）的方法造成大鼠认知功能障碍，Morris水迷宫行为学测试确定模型成功的动物，随机分成四组（每组12只：模型对照组给予等量水，盐酸多奈哌齐组（0.4mg/kg，体质量，下同），还脑益聪方低剂量组（7g生药／kg），还脑益聪方高剂量组（14g生药／kg），同时灌胃给药，并设立正常对照组，给予等量水。4周后，对大鼠行为学检测，应用酶联免疫法检测大鼠血浆P-选择素及PAI-1含量和血液流变学各项指标。

In virtue of these parameters, PID arithmetic using Hebb algorithm was applied to water temperature control. It was found that water temperature was precisely under control, and in disturbed state, well dynamic performance was achieved. A new method for optimum design of PID control based on Hebb algorithm for pasteurism temperature control was showcased in the paper.

在仿真研究所得参数的基础上，运用改进的有监督Hebb学习的单神经元自适应PID算法对热水箱水温进行控制，将其精确保持在目标温度，并且使其在有扰动的情况下具有良好的动态性能。

We came into this Creek in the Evening, resolving to swim on shoar as soon as it was dark, and discover the Country; but as soon as it was quite dark, we heard such dreadful Noises of the Barking, Roaring, and Howling of Wild Creatures, of we knew not what Kinds, that the poor Boy was ready to die with Fear, and beg'd of me not to go on shoar till Day; well Xury said I, then I won't, but it may be we may see Men by Day, who will be as bad to us as those Lyons; then we give them the shoot Gun says Xury laughing, make them run wey; such English Xury spoke by conversing among us Slaves, however I was glad to see the Boy so cheerful, and I gave him a Dram (out of our Patroon's Case of Bottles) to chear him up: After all, Xury's Advice was good, and I took it, we dropt our little Anchor and lay still all Night; I say still, for we slept none!

我们在傍晚驶进了小河口，决定一等天黑就游到岸上去，摸一下岸上的情况。但一到天黑，我们就听到各种野兽狂吠咆哮，怒吼呼啸，不知道那是些什么野兽，真是可怕极了！这可把那可怜的孩子吓得魂飞魄散，哀求我等天亮后再上岸。我说，"好吧，佐立，我不去就是了。不过，说不定白天会碰见人。他们对我们也许像狮子一样凶呢！"佐立笑着说，"那我们就开枪把他们打跑！"佐立在我们奴隶中能用英语交谈，虽然发音不太地道。见到佐立这样高兴，我心里也很快乐。于是我从主人的酒箱里拿出酒瓶，倒了一点酒给他喝，让他壮壮胆子。不管怎么说，佐立的提议是有道理的，我接受了他的意见。于是，我们就下了锚，静静地在船上躺了一整夜。

The results showed that the PCR fragment length of root-knot samples was 768bp in cowpea(RKN-1), tomato(RKN-2), balsam pear(RKN-3), Astragalus adsurgens (RKN-19)and rose(RKN-4) from Beijing, peanut fromWeifang (RKN-7) Yantai(RKN-6) and Jiaozhou(RKN-5) in Shandong, tomato from Hexian(RKN-9), cucumber from Suzhou (RKN-8) and Platycodon grandiflorum from Taihe(RKN-11) in Anhui and two kinds of tomato species(RKN-22,23) from China Central Agricultural University; the PCR fragment length of root-knot samples was 769bp in balsam pear from Ganyu(RKN-10) in Jiangsu, tomato from Kunming(RKN-12) in Yunnan, Langfang (RKN-17)in Hebei, Chengdu(RKN-18) in Sichuan and Suzhou(RKN-21) in Anhui and cucumber from Sixian(RKN-20) in Anhui; the PCR fragment length of root-knot samples was 772bp in guava(RKN-13) and pawpaw (RKN-15) from Hainan; the PCR fragment length of the other two samples was both 766bp in tomato(RKN-24,25) from China Central Agricultural University, and the PCR fragment length of root-knot nematodes in cucumber from Hangzhou in Zhejiang(RKN-16) and pepper from Anding (RKN-14)in Hainan was 767bp and 869bp, respectively.

结果表明，北京密云的豇豆、番茄、苦瓜，北京植保站月季，山东胶州，烟台和潍坊的花生，安徽和县的番茄，宿州的黄瓜和太和的桔梗，北京畜牧所沙打旺以及中国农业大学两个番茄品种(RKN-23和RKN-25)上的根结线虫种群的PCR扩增片段长度为768bp；江苏赣榆苦瓜，云南昆明，河北廊坊，安徽宿州和四川成都番茄以及安徽泅县黄瓜上根结线虫种群的PCR扩增片段长度为769bp；海南安定木瓜和石榴上根结线虫种群的PCR扩增长片段度为772bp；中国农业大学另外两个番茄(RKN22和RKN-24)品种上的根结线虫种群的PCR扩增片段长度为766bp；而浙江杭州黄瓜和海南安定胡椒上的根结线虫种群的PCR扩增长度分别为767bp和869bp。

There was a low accumulation of Pb in Pelargonium horlarum. When the Pb concentration in soil was increased by 1500 mg/kg, the Pb was accumulated by 169.5 mg/kg only. The plant content of Pb was lower than the hyperaccumulator critical content. The shoot' accumulation of Pb was significantly higher than that of root.

随着土壤中铅浓度增加，与对照相比，生物量在铅浓度增加1000 mg/kg时，才有显著下降；但对铅的吸收量较低，当土壤中铅浓度增加1500 mg/kg时仅吸收169.5 mg/kg；地上部分吸收量远小于地下部分的吸收量，转移系数小于0.5。

In the second part of the paper , oxidation of phenanthrene for the preparation of phenanthrenequinone by using halogen compound was studied . The chief factors of this method was discussed , and the optimum processing condition was obtained . The mechanism of the reaction by potassium hydroxide-precipitated was discussed , and the reaction kinetic parameters was measured .

论文第二部分研究了卤素化合物作为氧化剂氧化菲合成菲醌的方法，探讨了这种氧化方法的主要影响因素，从中找出较佳的工艺条件，并对其反应机理进行了初步探讨，测定了氧化反应的一些动力学参数。

Based on a great deal of tests, and through analysis of test result, experience on taming activated sludge was accumulated, operating parameters of integrated oxidation ditch which were best suitable for local condition were acquired. It was found that under the situation without solitude anaerobic zone, when the apparent anaerobic zone was absent, the strengthen biological phosphor removal phenomenon was really exist, and the phosphor removal efficiency was increasing with the increasing of BOD5/TP、 F/M.

在大量试验的基础上，通过对试验结果的分析，积累了培养活性污泥的经验，得出了最适合本地情况的一体化氧化沟的运行参数，发现了在没有单独设置厌氧区的情况下，在没有明显厌氧区段时，确实存在强化生物除磷现象，并且磷的去除率随BOD_5/TP、F/M的增大而增大。

The test was divided into three groups randomly as yh-16 - experiment group, pbs- blank control group and rpmi-1640 medium - positive group.③in the following 24h、48h and 72h interventions, the morphous of ec was observed by photology inverted microscope, the cytoactive was detected by method of mtt and the cell cycle was investigated by flow cytometry.the expression of vegf was dectected by real-time fluorescent quantitate polymerase chain reaction(real-time pcr).

以有效浓度的yh-16作用于ec，在不同时相点光镜下观察ec形态，mtt检测细胞活性，流式细胞术检测细胞周期，实时荧光定量pcr检测vegf的表达量。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。