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We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

The research results indicated that the best base was KOH, the best acid was H3PO4 and their neutralizing products were the necessary nutrients for foliar fertilizer; 12.4 L of foliar fertilizer were obtained in per kg of shrimp offal, chitin was obtained in 13.8% yield, crude protein was obtained in 23.2% yield using this method; This multifuctional foliar fertilizer was an abundant nutritional multifunctional foliar fertilizer which contained 15 kinds of amino acids, shimp cephalin, astacein and many trace elements. The concentration of calcium was 38.9 mg/L, the concentration of magnesium was 55.4 mg/L, the concentration of potassium was 20.0 g/L, the concentration of nitrogen was 4.5 g/L and the concentration of phosphorus was

研究结果表明：(1)处理虾下脚料的最佳碱是KOH，最佳酸是H3PO4，其中和产物是叶面肥必需的营养素；(2)本工艺不仅每Kg虾下脚料可制备虾叶面肥12.4L，而且可回收13.8％甲壳素和23.2％粗蛋白质；(3)经分析测定虾叶面肥中，含钙(Ca2+)38.9 mg/L，含镁(Mg2+)55.4 mg/L，含钾20.0 g/L，含氮4.5 g/L，含磷 78.9 g/L，还含有15种氨基酸及虾脑磷脂、虾红素及多种微量元素等，是一种营养丰富的多功能叶面肥。

So ist das Leben It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter o

首先，确定你的行业，和最关注的企业，把自己的简历放到人家的官方网站上（如IBM的官方招聘网址为http：// www-07.ibm.com/ employment/cn/index.html），然后定期去心仪企业的网站察看最新职位，并申请！

The results showed that the grain yield of winter wheat with two-irrigation was higher than that with one-irrigation with the same nitrogen application rate and method. Compared to one-irrigation,the grain number per spike in two-irrigation was higher,the 1000-grain weight was lower, the water wastage was higher, but the WUE was lower. The yield was not affected significantly by nitrogen rates and the methods. Within the nitrogen rate of 157.5-295.5kg/hm2, the lower NUPE and Nitrogen Productivity declined with nitrogen rate increasing. NHI was not lower than the others at the lowest nitrogen rate of 157.5kg/hm2. The accumulation of NO3--N in soil was significantly and positively correlated with the nitrogen application rate. NO3--N in one-irrigation was distributed mainly in the top layer (0-80cm), but it was inverse in two-irrigation.

结果表明，施氮量和施氮方式相同时春浇二水冬小麦的产量高于春浇一水，二水穗粒数较一水高，千粒重则低于一水，一水耗水量较少，水分利用效率明显高于二水；同一水分下不同施肥量及施肥方式对产量的影响差异不显著，同一施肥方式下在157.5-295.5kg/hm2范围内，施肥量越大，氮素生理效率和氮肥生产力越低，施氮157.5kg/hm2时，氮收获指数均处在最高或较高水平；土壤硝态氮残留量与施氮量呈显著正相关，成熟期一水条件下土壤中硝态氮主要集中在上层(0~80cm)，二水条件下则主要分布在中下层。

Ancient times the Babylon civilization located the environment belonged to the subtropics desert climate, but benefitted from two rivers, young sent pulls the bottom river and the Tigris River mother's gracious gift, was having the exceptionally broad impact plain, but by was in the arid few rain in the middle of the desert climate, on the one hand thus created the local wooded area to be very few, on the other hand was the great plains which so stretched to the horizon smoothly like baffles, from this has become the sierra absolute forbidden area, from this occupied the plain center region people, even if wanted to use the firm stone material, also could because was far away, either was gives way before difficulties looks Shi Xing to sigh, either was after pays the extremely soaring price, shipped in by farSeveral stones only can use in actually carving the magnificent precious tombstone, even was in some plain person all not once has seen the mountain appearance for a lifetime, in brief took root in two river basin Cuban Billen civilization, finally is framed was the soil texture civilization, they created the cuneiform character, all could not but engrave above the thin clay tablet, was recording the exceptionally precious history, obviously in the different region humanity, all was influences subtly sparely no effort regarding the side each kind of environment resources use.

古代巴比伦文明所处的环境属于亚热带沙漠气候，但得益于两条河流，幼发拉底河与底格里斯河母亲般的恩赐，拥有着异常广阔的冲击平原，但由处于干旱少雨的沙漠气候当中，从而造成一方面当地森林面积很少，另一方面就是如此一望无垠平坦如砥的大平原，由此成了山脉的绝对禁区，由此处在平原中心区域的人们，即使想使用坚固的石头材料，也会因为路途遥远，要么是知难而退望石兴叹了，要么是在付出极为高昂的代价后，远远运来几块石头却只能用于雕刻华丽珍贵的墓碑，甚至是有些平原中的人一辈子都不曾见过大山的模样，总之植根于两河流域的古巴比伦文明，最终被定格为土质文明，就连他们所创造的楔形文字，都不得不刻印在薄泥板之上，来记载着异常珍贵的历史，可见不同区域内的人类，对于身边各种环境资源的利用都是潜移默化不遗余力的。

The geology research of carbonate reservoirs in Ordovician of Tahe oilfield showed that many large dissolved vugs and fractures were developed. The spread of the reservoir take on a very heterogeneous state. And the typical fractured-vuggy reservoirs was formed. According to the combination of the various spaces, the formation was divided into vuggy formation, fractured-vuggy formation and fractured formation. The research showed that development of the vug is relevant to the its position on the structure, the fractures, the water surface and the weathering surface. Multi-phase charge and recharge of the oil in the reservoirs resulted in the complexity of the fluid distribution. So the"Bottle Model"was brought forward to explain the movement of the water-oil contact. The special storage space and the complex fluid distribution lead to the unstability of the production and the complexity of the water cut. In order to explain the characteristic of the fracture-vug unit was brought forward and the basic principle and method of the partition of the fracture-vug unit was formed. The classification and evaluation of the units were performed according the reserve and energy. Based on the research of geology characteristic and the fluid flow in the reservoir the comprehensive numerical simulation plan of the fractured-vuggy reservoirs were established. Through the selection of the simulation unit, fluid flow type and parameter equivalent the triple media reservoir simulation model was established. Considering the practical application the model was resolved by the DKR decomposition conjugate grads method. Based on the fine reservoir description of Dsitrict IV of the Tahe oilfield the various space type were classified and combined together. The geology model of Unit S48 was constructed. Two typical single well model was established according to the geology and the dynamic phenomena. Finally the single well model and the Unit S48 were simulated by the triple media reservoir simulator. Via the local grid refinement and coarsening in the simulation good matchs were gained. Based on the results of the simulation the reserve distribution, percent of reserve produced in various space and the natural energy were analyzed. The fluid was storaged in the fractures and vugs mostly and more than 90% the produced oil came from the systems. The energy belonged to the active one. The results of simulation accorded with the fact and showed the validity and practicability the research and the simulator.

塔河油田奥陶系碳酸盐岩油藏的地质研究表明，其储层中发育着很多大型的溶蚀洞、缝，储层的平面展布呈现出极度的非均质性，形成了典型的缝洞型碳酸盐岩油藏，根据各种孔隙介质在储层中的组合，将储层分为了溶洞型、裂缝-溶洞型和裂缝型三类；研究表明溶洞储层的发育和构造位置、裂缝的发育、潜水面和风化面的位置等因素有关；多期充注的油气成藏模式导致了流体复杂的赋存状态，由此提出了所谓的"瓶子模型"，解释生产过程中油水界面的变化；特殊的储集空间类型和流体分布特征导致油田在开发过程中表现出很大的不确定性和含水变化的复杂性，为了合理的解释油气田开发过程中的动态特征，提出了"缝洞单元"的概念，并制定了"缝洞单元"纵横向划分的基本原则和依据及划分方法，并对"缝洞单元"进行了分类和评价；基于地质特征和流体在其中流动规律的研究，提出了缝洞型碳酸盐岩油藏的数值模拟综合解决方案，通过模拟单元的选择、流动类型和参数的等效，建立了三重介质油藏三维三相数值模拟模型，采用不完全LU分解预处理共轭梯度法进行了求解；在塔河油田4区精细油藏描述的基础上，将各种类型的孔隙空间进行了归类组合，建立了S48单元的地质模型；通过对油井生产动态进行分析研究，建立了两类和油井地质、生产动态相对应的单井模型；最后应用三重介质油藏数值模拟软件对单井模型和S48单元进行了数值模拟，通过局部加密和粗化等技术模拟流体流动规律，取得了很好的拟合效果；结合数值模拟结果，分析了各种介质中的储量分布、储量产出的百分比以及地层的能量，认为塔河油田缝洞型油藏中流体绝大多数储集于缝洞系统之中，所产出流体90%以上也来自于缝洞系统，其底水能量属于较充足的类型；模拟结果和油田实际情况符合较好，说明了地质研究和油藏数值模拟研究的正确性。

The results showed that the most suitable time to isolating the Larva from the experiment animals, which were experimentally infected with canine Trichinella was on the fiftith day; the most suitable time of degesting was at 37℃ for 17 hours; 38℃ for 14 hours; 39℃for 19 hours; 40℃ for 10 hours; the most suitable inquantity to inoculate per millilitre was 6000 strips; the best temperature was at 37℃; the best gas condition was in the CO2gas incubator and the concentration of CO2 was 8 per cent; the most suitable pH was at 7.0; the most suitable medium was RPMI-1640; the best nourishment component was peptone; the most appropriate time interval of changing medium was about 24 hours; the best time to observe was at 24 hours.

试验结果表明，从人工感染犬旋毛虫的实验动物中分离肌幼虫的最适日龄为50天，最适虫体消化时间为37℃时17h、38℃时14h、39℃时12h、40℃时10h，每ml培养液最适虫体接种量为6000条，最佳培养温度为37℃，最适培养气体条件为CO_2二氧化碳培养箱、最适培养液pH值为7.0，最适培养基为RPMI—1640，最佳培养液附加营养成分为蛋白胨，最佳培养液更换时间间隔为24h，最适观察虫体时间间隔为24h。

The average level of the soil available potassium was 119.96 mg/kg, which decreased 18.94 mg/kg and 1.05 mg/kg one year averagely. But the decreasing trend alleviated in recent years. There were 39.5 kg/hm^2 nitrogen and 30.9 kg/hm^2 phosphorus (P2O5) more than needed averagely, and 150.0 kg/hm^2 potassium (K2O) in deficiency. Though the fertilizer application was high and the rate of fertilizer was 647.96 kg/hm^2 in nutrient, the ratio of organic to inorganic was still unreasonable. The ratio of the chemical fertilizer nitrogen phosphorus (P2O5) and potassium (K2O) was 1:0.42:0.14. The nitrogen application was enough and even higher than that was needed. The phosphorus was proper elementarily and potassium was in deficiency. The yield of wheat which was the main crop increased slightly and that of other autumn harvest crops was stable.

土壤速效钾7年平均值119.96 mg/kg，比第二次土壤普查时下降了18.94 mg/kg，平均每年以1.05 mg/kg的速度下降，但近年来下降趋势变缓；养分平衡中氮素平均盈余39.5 kg/hm^2，磷素(P2O5)盈余30.9 kg/hm^2，钾素(K2O)亏缺150.0 kg/hm2；全省施肥水平较高，每公顷总施肥量折纯养分647.96 kg，但有机无机比仍不合理，化肥氮、磷、钾投入比例1：0.42：0.17，氮肥充足，部分地区用量偏高，磷肥基本合理，钾肥不足；主要农作物小麦产量略有上升，其它主要秋收作物产量基本稳定。

"It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way--in short, the period was so far like the present period, that some of its noisiest authorities insisted on its being received, for good or for evil, in the superlative degree of comparison only."

这是一个最好的时代，这是一个最坏的时代，这是一个智慧的时代，这是一个愚蠢的时代，这是一个信任的时代，这是一个不信任的时代，这是一个光明的时代，这是一个黑暗的时代，这是一个希望的春天，这是一个令人绝望的冬天，我们拥有任何事之前，我们没有拥有任何事之前，我们有一切去天堂的路，我们有一切短的路，这个时代就是这麽遥远的像现在的时代，那某一个属于它的权力被他坚持存在，为了最好的或者为了最坏的，在这个最好的度属于唯一的。

"It was the best of times, it was the worst of times, it was the age of wisdom, it was the age of foolishness, it was the epoch of belief, it was the epoch of incredulity, it was the season of Light, it was the season of Darkness, it was the spring of hope, it was the winter of despair, we had everything before us, we had nothing before us, we were all going direct to Heaven, we were all going direct the other way--in short, the period was so far like the present period, that some of its noisiest authorities insisted on its being received, for good or for evil, in the superlative degree of comparison only."

&这是最好的时代，这是最坏的时代，是智慧的时代，这是愚蠢的时代，这是时代的信仰，这是划时代的怀疑，这是光明的季节，这是黑暗的季节，这是春天的希望，这是绝望的冬天，我们都摆在我们面前，我们没有任何关系摆在我们面前，我们都将直接向天堂，我们都将直接的其他方式，在短期间，是迄今为止像本期间，它的一些噪音当局坚持其正在收到良好的或邪恶的，在高级程度的比较只。&

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了