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The results summarized as follows:(1)The main pharmacokinetic parameters Tmax was about 2h, Cmax was 8.365, 7.519, 1.871, 6.485, 4.060μg/mL in blood, gill, kidney, liver, and muscle respectively, after oral administration at a dose of 30 mg/kg; while t1/2 were 8.180~23.249h. These showed that the NFLX absorption was rapid after oral administration of NFLX in Scophthalmus maximus, but the residence time in the fish was long.(2) T1/2 were 74.88~200.45h after oral administration at dose of 30 mg/kg for five consecutive days, which showed that the attenuation of NFLX in the fish body was slow and the residue concentration in the kidney was the highest.

另外，本文测定了大菱鲆成鱼体内NFLX的药物浓度，用MCP-KP药动学程序对所得的药物浓度-时间数据进行分析，结果表明：1单次混饲口灌(30 mg/kg体重日)NFLX，药物在大菱鲆体内的达峰时间为2h，血液、腮、肾脏、肝脏、肌肉达峰浓度分别为：8.365、7.519、1.871、6.485、4.060μg/mL，消除半衰期为8.180～23.249h，说明NFLX在大菱鲆体内吸收较迅速，但滞留时间较长。2连续五天混饲口灌(30 mg/kg体重日)NFLX，消除半衰期为74.88～200.45h，说明混饲口灌NFLX在大菱鲆体内消除缓慢，其中以肾脏中残留最显著。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

According to research on space between wheel and Cart rut traverse curve of single roadway, the width of strip slab was fixed. When there was horizontal loading on slab, contact state between strip slab and base course was studied, and finite element model was established. Comparing maximum principle stress among different load position on slab, it was found that the critical loading position of cement concrete strips pavement was the middle of longitudinal joint.

对车辆轮距和单车道轮迹横向分布曲线进行分析，确定轮迹板板宽；在有水平荷载作用时，对轮迹板与基层的层间接触状态进行分析，建立有限元计算模型，对比荷载作用在轮迹板不同位置时的最大主应力，最终确定水泥混凝土轮迹路面板的临界荷位为纵缝边缘中部。

The efficacy of photocatalytic oxidation was relative to the number of dip-coated plate, which 2 and above plates was superior to one plate, and also vertical plane to the light source was more efficient than parallel plane. The reaction rate constant showed the color removal decreased as initial MB concentration was raised. As for efficiency of catalyst durability, it was found that TiO2 dip-coated stainless steel plate performed 95% color removal at 5ppm MB even after 4 times repeated usage, which showed favorable durability. However, when the concentration up to 10 ppm, the color removal decreased as the used counts was raised.

脱色率和钢网片数有关，钢网片数超过2片以上时，其光催化效能优於单片者；光催化效能与钢网摆放方式或光源入射的角度有关，与光源垂直摆放的脱色率优於与光源平行摆放者；由反应速率常数得知，反应物之脱色率随著反应物初始浓度的提升而递减：在耐用性方面，亚甲基蓝5ppm在重覆使用四次后，其六个小时脱色率皆维持95%，显示自制光触媒钢网之耐用性颇佳，若将反应物浓度提升至100ppm，其脱色率随著使用次数之增加而递减。

The result showed that the spatial variability of the soil bulkinthis area was complicated and there was not a certain variation.The soil bulk density of loamy soil was(1.55±0.09)g/cm3,the soil bulk density of sandy soil was(1.43±0.16)g/cm3 and the soil bulk density of clayey soil was(1.54±0.07)g/cm3.The soil bulk density of the cross-bedding soil composed by sandy soil and clayey soil was(1.47±0.12)g/cm3.The soil showed a tendencyto be compacted.

结果显示，研究区域土壤容重的空间变异情况复杂，无确定的变化规律；壤质类土壤的容重值为(1.55±0.09)g/cm3，砂质类土壤的容重值为(1.43±0.16)g/cm3，粘质类土壤的容重值为(1.54±0.07)g/cm3，粘砂交错层的土壤容重值为(1.47±0.12)g/cm3，土壤呈紧实趋势。

Eric who has many years' driving experience has had many car accidents. The most serious accident changed his attitude towards driving tremendously."In that day I was driving past Sai Kung and was speeding on the big slope in Pik Uk. I was driving at a very high speed. As a result since the ground was slippery, the tires aquaplaned. The car made three turns of 360 degrees before It could come to a stop. The whole car was ruined and almost fell off the mountain. Luckily I was not hurt." Thereafter Eric did not pursue high speed.

已有多年驾驶经验的Eric，也试过不少交通意外，最严重的一次，令他的驾驶态度产生大改变，「当日驾车经过西贡，在壁屋的大斜路上飞驰，当时的车速颇高，结果因地面湿滑而跣胎，打了三个三百六十度的圈才停下，全车尽毁，差点掉下山，庆幸自己冇事。

Results The anterior bundle of ulnar collateral ligament originates from the inferior aspect of the medial epicondyle and inserts immediately adjacent to the joint surface on the ulna near the sublimis tubercle. The posterior bundle originates from the medial epicondyle slightly posterior to its most inferior portion and inserts broadly on the olecranon process. The lateral collateral ligament arises from the inferior aspect of the lateral epicondyle. Two types of conjoined lateral collateral and annular ligamentous insertions on the ulna were observed. Type Ⅰ(61.2％) was bilobate and type Ⅱ(38.8％) was a single broad conjoined type with insertion on the ulna. The anterior band of anterior bundle was more tighten than the posterior band as the elbow flexed less than 60°. When the elbow flexed over 60°, the two parts of anterior bundle were equally tightened. The posterior bundle was tightened as the elbow was flexed more than 90°. The lateral collateral ligament was tightened gradually as the elbow moved in flexion.

结果(1)尺侧副韧带前束起于肱骨内上髁的前下方，止于尺骨冠突内侧的小结节；后束起于肱骨内上髁的内下方，止于尺骨鹰嘴内侧的骨面；(2)桡侧副韧带起于肱骨外上髁的外下方，其纤维部分止于环状韧带，部分止于尺骨冠突的外下方；桡侧副韧带和桡骨环状韧带在尺骨上的止点有两种类型：Ⅰ型占61.2％，桡侧副韧带的部分纤维汇于环状韧带的尺骨止点，另一部分纤维单独止于稍远的尺骨上；Ⅱ型占38.8％，桡侧副韧带和环状韧带形成一宽的纤维止于尺骨上；(3)在肘关节屈曲60°以前，尺侧副韧带前束的外侧部紧张而内侧部较松弛，肘关节屈曲超过60°后，前束内外侧处于同程度的紧张状态；尺侧副韧带的后束在肘关节屈曲超过90°后才被拉紧；(4)桡侧副韧带在肘关节不同屈曲状态时其紧张度逐渐增加，当肘关节屈曲超过90°时，桡侧副韧带被明显拉长。

This study discovered that total amount of potential medication safety risks was 1,876 and the overall prevalence was 7.3%. The items of results depending on categorizes of evaluation, ward, medical specialty, diagnosis and pharmacology were as follows: The highest prevalence is pharmaceutical incompatibilities which is 4.7%. The highest prevalence was general ward which is 4.3%. The highest prevalence was pulmonary disease which is 2.5%. The highest prevalence was acute respiratory failure which is 3.0%. The highest prevalence was cathartics and laxatives which is 1.6%.

研究结果发现这群管灌病人的口服剂型治疗药物中具潜在用药安全性风险问题的件数为 1，876 件；整体平均盛行率为 7.3 %，其中依风险问题评估类别、病房别、科别、疾病主诊断别、药理作用类别之各项统计分析结果，分别占居最高盛行率者为：药剂学性配伍禁忌之盛行率为 4.7 %；一般病房之盛行率为4.3 %；胸腔内科之盛行率为 2.5 %；急性呼吸衰竭之盛行率为 3.0 %；泻剂及缓泻剂类之盛行率为 1.6 %。

Results The main structure of the left ventricle in canine was composed of the mitral complex、aortic valve、falsetendons and so on,among them the mitral complex was included in the mitral ring and valve,chordae tendinease and papillary muscles;the circle of mitral ring was 49.16±10.00mm,the average heights of inside and outside valves were 14.28±3.14mm,9.27±1.99mm,respectively,the breadth were 25.74±5.21mm、21.50±6.33mm;the average number of chordae tendineass was 16.72±2.76;the average number of left ventricular false tendon was 4.69±3.38:the circle of aortic valve ring was 34.55±8.00mm,the average heights of left、right and back aortic valves were 6.85±1.76mm、7.19±1.75mm and 7.52±1.79mm.

结果：犬心左心室主要结构有二尖瓣复合体、主动脉瓣、假腱索等，其中二尖瓣复合体由瓣环、瓣膜、腱索、乳头肌构成；二尖瓣瓣环周径为49.16±10.00mm，内侧瓣、外侧瓣的高度分别为14.28±3.24mm、9.27±1.99mm，其宽度分别为25.74±5.21mm、21.50±6.33mm；腱索总数平均为16.72±2.76条；假腱索总数平均为4.69±3.38条；主动脉瓣瓣环周径为34.55±8.00mm，左瓣高度、右瓣高度和后瓣高度分别为6.85±1.76mm、7.19±1.75mm和7.52±1.79mm。

The umbilical cord blood solution, which the DMSO concentration is 1.0M, 1.5M and 1.75M,was confected. The solution was in equilibrium for 20 minutes. Then, the umbilical cord blood was completely immerged in liquid nitrogen for about 24 hours. Then, the umbilical cord blood was taken out and was warming in the thermostat water bath for 20 minutes. The fraction surviving of the umbilical cord blood was more than 80%.

先将含有细胞的培养液、0.9％的NaCl和2.0M的DMSO溶液配制成分别DMSO浓度为1.0M、1.5M、1.75M的脐带血干细胞溶液，将配好的玻璃化溶液置于4℃静置20分钟使脐带血干细胞的玻璃化溶液达到细胞内外溶液平衡，然后将其投入液氮中大约24小时左右，最后将液氮中的细胞取出37℃水浴复温，细胞存活率达到了80％以上。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。