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The works in the project just done can be summarized as five respects.(1) A device was designed to fabricate the asymmetrical resonant cavities by a pendant drop. A series ARCs with variable parameters provides a valuable experimental method for investigating the characteristics of ARCs.(2) The relationship between the number of active modes and the shape parameters in an ARC was investigated. The experimental lasing threshold was compared with theoretical result, the good agreement between two shown that it was the decrease of the number of active modes in an ARC that decreased lasing threshold.(3) Dye lasing gain was used to enhance the signals of stimulated Raman scattering of the weak gain Raman modes in a circular cavity made by a pendant drop, the instantaneous SRS spectra of weak gain Raman modes in ethanol was obtained by this new method.(4) The SRS signals of minority compound in a binary chemical complex was obtained by using dye lasing gain method. The detection concentration of minority compound was reduced to an order compared with normal SRS method.(5) A cylindrical circular cavity was formed by a liquid jet, which was used to reduce the pump intensity required for laser induced plasma spectroscopy. The limit of detection of trace element in aqueous sample was greatly decreased by this new method.

我们设计并成功制作了使悬垂液滴连续变形的装置，用此装置可以方便地改变非圆对称谐振腔的几何参数，为ARCs的研究提供了一种有效的实验方法；研究了ARCs中活性模式数和变形参数间的变化规律，激光阈值的数值计算结果和激光辐射的实验结果作了比较，二者吻合的曲线说明ARCs中活性模式数量的减少是受激辐射放大阈值降低的原因；在由单元化合物构成的微型圆对称谐振腔中，首次尝试用"激光增益"增强弱增益拉曼模式的受激拉曼散射信号，获得了乙醇分子中弱增益拉曼模式的瞬态SRS光谱；在由二元混合物构成的微型圆对称谐振腔中，用染料的激光增益增强了少量化合物的SRS信号，把少量化合物的SRS信号探测浓度降低了近一个数量级，在SRS应用于瞬态过程的组份分析方面做了卓有成效的探索；用石英毛细管形成的稳定液拄构成拄形谐振腔，降低了激光诱导等离子体光谱需要的激励能量，有效地降低了水相样品的痕量元素分析极限，为LIPS应用于微量元素的化学分析开拓了一条新的技术路线。

In this thesis the process of constructing the non-perturbative Hamiltonian theory is de-scribed and is applied to estimate the vacuum condensate. It contains the following contents:At the very beginning, by using the path integral method and eliminating the gluon freedom, aGCM action 〓 of current quarks including lower order current-current coupling was derivedfrom the QCD Lagrangian and the effective Hamiltonian operator that could hardly be doneby the normal methods was derived. After doing this, the broken vacuum is introduced whichincludes quark-antiquark condensate through the generalized Bogoliubov-Valatin transformation,the effective Hamiltonian of constituent quark was derived. The detailed formulas containingthe spatial current-current coupling term for the effective Hamiltonian and gap equations wasworked out by parameterizing the correlation kernel as a quadratic potential. And then, the gapequation was solved and the quark-antiquark condensate of vacuum was studied both in the casesof instantaneous interaction and retarded interaction. In the end, the effective Hamiltionian withtwo-body quark-quark interaction was derived with one-body approximation, and with the helpof the functional integral method the coupling non-linear dynamic equations for systems withnuclear matter was derived. Finally, these equations were solved by selfconsistent method andthe effect of nuclear matter on vacuum condensate was studied. The spatial current-current coupling term is too difficult to handle, hence the correlationkernel is assumed to be not important and usually omitted in the pure vacuum condensate, andthe instantaneous interaction generally is adopted. Retaining the spatial current-current termand partial retardation effect, the quark pairs condensate in pure vacuum was studied, and theeffect of quark mass was also studied. At present, little study is focused in the case with nuclearmatter and spatial current-current term also omitted. Under the approximation with partialspatial current-current term, the effect of nuclear matter on vacuum condensate was studied.

本论文描述了量子色动力学整体色对称模型哈密顿量方法的构建过程，得到了反映正反夸克对凝聚真空结构的关于组分夸克的有效哈密顿量算符，它隐含了胶子作用，并且准确至流-流耦合项；接着，通过参数化哈密顿量中的夸克作用关联核，导出平方禁闭势参数化选择的哈密顿量的具体公式和能隙方程；随后，应用公式，编程求解，考察了瞬时作用下和部分延迟作用下真空的正反夸克对凝聚，在计算中保留了空间流-流耦合作用；之后，导出瞬时势和延迟势下包含二体作用项的哈密顿量公式，并采用单体化近似，通过泛函变分方法得到核物质存在时耦合的非线性动力学方程；在保留部分空间双流耦合作用的近似下，求解核物质的动力学方程，考察核物质密度对真空凝聚的影响，以往考察真空凝聚，对关联核的选用，由于空间流-流耦合项不易处理，也认为作用不大，常忽略该项，并且常采用瞬时作用；本文保留空间双流项和部分延迟作用，考察了真空情形的夸克对凝聚，还考察了夸克质量对纯真空凝聚的影响，以往对核物质存在情形的真空凝聚考察很少，也都忽略空间流-流项，本文在考虑部分空间流-流项近似下，考察了核物质存在对真空凝聚的影响。

Bob lives together since childhood with Harker is adding the iris town, was the Harker childhood's playmate, the stature is not high, the hair from was born curls the volume, the eye was very big, the skin was very white, always likes putting on the suspenders trousers' his stature not to be high actually has the keen skill, the present is the brave warrior number operates the rudder, raised casually in here, the brave warrior number was their pirate ship, was also their family, in here, had the partner billy always to put on the identical set of western-style clothes, not the big bow tie, he was very actually graceful, high stature, fair skin, beautiful blue color eye, high bridge of the nose, will smile will let the human remember the fine thing, he will have gentleman's quiet and tasteful, also will have free man's profligate being unable to restrain, no matter which young lady let perhaps the woman looks could be infatuated with his -- him to be skilled in the seacraft, could precise draw each sea area the sailing chart, he was the brave warrior number navigation teacher, partner Du duly, the braid has covered entirely on his head, his eye a little looked like a bean, both hands is not big, actually could make compared to imperial palace delicacy food, what was worth mentioning, he was a thin person -- also has the partner to love Milly, he was Bob's younger sister, beautifulLong curl, big and attractive brown eye, long, but curls the eyelash, the beautiful skin, the beautiful beautiful fascinating' winsome's stature dances lightly in the sea!

鲍比从小和哈克一起生活在加芝镇，是哈克小时候的玩伴，个子不高，头发从出生来就卷卷的，眼睛很大，皮肤很白，总是爱穿背带裤的他个子不高却拥有灵敏的身手，现在是勇士号的掌舵者，随便在这里提一下，勇士号是他们的海盗船，也是他们的家，在这里，有伙伴比利总是穿着同一套西服，却不大领结，他很帅，高高的个子，白皙的皮肤，美丽的蓝色眼睛，高高的鼻梁，笑起来就会让人想起美好的东西，他有绅士的幽雅，又有自由者的放荡不禁，不管让哪个小姐或是妇人看了都会迷上他——他精通航海术，能够精确的绘出各个海域的航海图，他是勇士号的航海师，伙伴杜杜利，辫子布满了他的头上，他的眼睛有点像一颗豆，双手不大，却能做出比皇宫更美味的食物，值得一提的是，他是个瘦子——还有伙伴爱米利，他是鲍比的妹妹，美丽的长卷发，大而漂亮棕色的眼睛，长而卷的睫毛，美丽的皮肤，美丽婀娜的身材在大海中翩翩起舞！

Results Positive rate of Amsel was 28%, one step sialidase was 34%, Gram-stain was 30% and amines was 24%. Using Amsel as criterion, the sensitivity of one step sialidase and amines was 92.9% and 71.4%; specificity was 88.9% and 94.4%; positive predictive value was 76.5% and 83.3% negative predictive value was 97% and 89.5%, using Gram-stain method as criterion, the sensitivity of one step sialidase and amines was 93.8% and 66.7%, specificity was 92.1% and 94.3%, positive predictive value was 83.3% and 83.3%, negative predictive value was 97.2% and 86.8% respectively.

Amsel法阳性率28%，组织多胺试验阳性率24%，一步法唾液酸酶活性的检测试验阳性率34%，革兰染色细菌评分法阳性率30%；唾液酸酶活性检测法与Amsel法，革兰染色细菌评分法相比较差异无显著性；组织多胺试验与Amsel法，革兰染色细菌评分法相比较差异无显著性；以Amsel法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为92.9%和71.4%，特异性分别为88.9%和94.4%，阳性预期值分别为76.5%和83.3%，阴性预期值分别为97.0%和89.5%；以革兰染色细菌评分法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为93.8%和66.7%，特异性分别为92.1%和94.3%，阳性预期值都为83.3%，阴性预期值分别为97.2%和86.8%。

This dissertation was focused on the research about the formation and evolution of NQB from the Late Triassic to the early middle Epoch of Jurassic Period, including integrated analyzing the characteristic of the key stratigraphic surfaces, the time of the key stratigraphy, regional rock straigraphy, biostratigraphy and chronostratigraphy, sedimentary system and depositional facies. On basis of these analysis, a tectonic evolution model have been established for NQB during from the Late Triassic to the early middle Epoch of Jurassic Period.1. After the systematic surface analysis about main stratigraphy units, we found that:(1) the bottom of Xiaochaka formation was comformity with the middle Triassic in the basin, and in the north margin it was deposited and overlapped on the different period stratigraphy before Triassic.(2) The top surface of Xiaochaka formation developed regionally karst and paleo-karst breccia, and was uncomformity with Nadigangri Formation, these things suggest that NQB in Xiaochaka depositing stage was developed from middle Triassic basin, and then an extensive regression occurred, the deposition was over and erosion come into being.(3) The lithologic assemblage, geochemistry, mixed fossils, and stratigraphy overlapping characteristic of Quemocuo Formation in early middle Jurassic indicted that the uncomformity surface was an extension-unconformity.2. Integrated analysis about stratigraphy division and correlation indicted that:(1) the paleontologic assemblage of Xiaochaka formation show its time was Carian -Norian stage of late Triassic. According to it was overlapped by late Triassic Nadigangri Formation, so Xiaochaka formation should be Carian - early Norian stage.(2) the SHRIMP Zircon U-Pb age of Nadigangri formation volcanic rocks wear vary form 216 to 205 Ma, so its time should be Norian, but maybe extended into Rhaetian Stage.(3) The age of its bottom volcanic rocks, the fossil assemblages of its middle and upper members and overlapped by lower Jurassic, indicted the age of Riganpeicuo Formation was Norian - Rhaetian stage. And the same time, the volcanic rocks geochemistry of Nadigangri and Riganpeicuo formation both reflected that they wear formed in rift tectonic settings. The lower lithologic and surface characteristic, fossil assemblages and its upper maybe was conformity with lower Jurassic, all reflected that the age of Tumengela Formation in Tumen area should be Norian - Rhaetian stage.

论文对晚三叠世—中侏罗世早期盆地的形成与演化开展了研究，包括关键地层界面特征分析、关键地层时代归属分析、区域岩石地层、生物地层和年代地层的综合对比分析、区域沉积体系与沉积相分析、岩相古地理分析，最终建立晚三叠世—中侏罗世早期羌塘盆沉积—构造动力演化模式。1、系统的对各地层单元的界面分析发现：(1)肖茶卡组底界在盆地内部与中三叠统整合关系，在盆地北缘为沉积超覆于不同时代的前三叠系之上：(2)肖茶卡组顶界面发育古岩溶角砾岩，与上覆那底岗日组为岩溶不整合接触，说明肖茶卡组沉积时期的羌塘盆地是在早中三叠世的盆地基础发展起来的，后期出现了广泛的海退事件，使盆地结束沉积并遭受风化剥蚀作用；(3)中侏罗统雀莫错组的岩性组合特征、岩石化学特征、古生物化石混积和地层的沉积超覆现象均表现出裂陷环境下形成的伸展不整合面的特征。2、综合地层划分与对比分析认为：(1)肖茶卡组古生物化石组合反映其时代为晚三叠世卡尼期—若利期，根据其上被上三叠统那底岗日组不整合覆盖，将其时代厘定为卡尼期—若利期早期；(2)那底岗日组火山岩SHRIMP锆石U-Pb年龄在216～205 Ma之间，其时代主要为若利期，可能包含有瑞替期：(3)日干配错组底部火山岩的年龄、中上部化石组合及其被下侏罗统整合覆盖，综合反映的时代为晚三叠世若利期—瑞替期，而且那底岗日组火山岩和日干配错组火山岩的地球化学成分分析反映两者均为引张构造背景下的产物；土门地区的土门各拉组的下部岩性及界面特征、化石组合和上部可能被下侏罗统整合覆盖，综合反映其时代为若利期—若利期。

The UV and IR spectroscopic studies of the monomer films showed that the photoreaction ratio of monomer (about 50%) was higher than the corresponding photosensitive polymer (about 10%, PVCi). The polarized IR spectra proved that obvious anisotropic absorption was displayed in the irradiated photosensitive film. With polarizing microscopy method, we found that these kinds of photoalignment films exhibited good alignment ability, and the stability of the photoalignment film could reach about 100℃. 2 A photosensitive self-assembled monolayer was fabricated that contained photo-cycloadditable groups, such as, coumarin and cinnamate. The reaction process were detected by contact angle method because the IR absorption of monolayer was very weak. The photosensitive self-assembled monolayer promote uniform alignment of nematic liquid crystals by LPUV, and it thermal stability could reach about 130℃. 3 A cationic bolaform amphiphiles was synthesized that had two photosensitive double bonds, and can be polymerized by ultraviolet. A layer-by-layer self-assembled deposition film was prepared in aqueous solution by using this material and poly (sodium 4-styrenesulfonate). The UV-Vis. spectra proved that the multilayers film was uniform and layer-by-layer. When LPUV irradiated the film, the photopolymerizable double bond underwent [2+2] cycloaddition, and the production of the reaction distributed along the polarized direction of incident LPUV. Polarized UV-Vis. spectra indicated that the irradiated self-assembled multiplayer film was anisotropic, and the dichromic ration is about 0.09, it is higher than the spin-coating method of photosensitive polymer or monomer. We used this anisotropic film as alignment layer of nematic liquid crystals, and obtained a uniform and stability alignment result, the static contrast ratio is about 100, which is very close to application of liquid crystal device. This thesis is an instance that the photochemical material was used in the physics region of liquid crystals, the route that was from photosensitive monomer, self-assembled monolayer film to self-assembled multiplayer film was undergone, by this logical research method photoailgnment of liquid crystal was extended and consummated.

围绕上述思路开展了如下工作：1合成了三种可进行光二聚反应的光敏小分子单体，LPUV 辐照制作了光控取向膜；用紫外-可见光谱法和红外光谱法分析了薄膜中的光化学反应，发现小分子的光反应程度可达到50%以上，至少比光敏高分子高出5倍；薄膜的偏振红外光谱证实，经过LPUV 辐照后薄膜出现了明显的各向异性；将小分子光控取向膜制成液晶器件，在偏光显微镜下观察看到均匀一致的液晶取向；并且热稳定性达到100℃左右，接近实用化要求。2制备了含肉桂酸、香豆素基团的自组装单层膜；采用接触角的方法监测自组装反应的动力学过程；紫外-可见光谱证实了自组装单层膜在石英基板表面形成；自组装单层膜经LPUV 辐照后能诱导液晶均匀取向，而且热稳定性可达130℃，热稳定性进一步提高。3合成了含有光敏双键的双季铵盐，在水溶液状态下将它和聚乙烯基苯磺酸钠沉积生成layer-by-layer 自组装多层膜，紫外-可见光谱证实这是一个逐层均匀的沉积；在LPUV 照射下，自组装膜中的光敏双键按照偏振方向发生[2+2]环加成反应，形成取向膜，用二向色性值评价的分子取向度高于高分子及小分子光控取向膜的水平；实验证明用自组装多层膜制成的液晶器件亮暗态均匀，静态对比度约为100，达到实用化器件的水平。

Model of animals were established by putting rats in a container with slippy inner wall and half-full of water in it, letting them swimming freely till died of being drowned. Each rat from different groups was killed by breaking its neck except drowning group, then BW and SO_2 was detected, and its chest was cut open rapidly, lung was taken out and weight was measured accurately to count lung coefficient. Its lung appearance was observed and lung tissue was fixed with formalin, which was made into pathology slices with HE staining, and pathology change of the lung tissue was observed;Expression of IL-8 and IL-10 in lung tissue was detected with Immunocytochemistry, and correlation between them was analyzed .

动物模型制作采用将大鼠放入盛有黄河水的内壁光滑的容器内，让其自由泳动自然溺死，除溺水致死组外，各组动物分别在规定的时间点断头处死，行体重和血氧饱和度等指标检测，并在冰盘中迅速开胸取肺，准确称重以计算肺系数，观察各组大鼠肺脏外观，取肺组织福尔马林固定，制成病理切片HE染色，光镜下观察肺组织病理改变；用免疫组化的方法检测肺内IL-8、IL-10的表达，并对其相关性进行分析研究。

In the first stage, when environment temperature arrived to 37.5℃, Ta of A and B dairy barn was 35.5 and 36.7℃, respectively. And at the same time Tr was higher than 39.5℃. Specially, the high yield dairy in A barn raised to 41 ℃ at 22:00 and RR was 90/min, the RR of low yield dairy was 65/min at this time. mTs was 37.6℃ at 15:00. In the third stage, Ta of the barn was higher than outside when outside Ta was lower than 27.6℃. When the ambient temperature was lower than 28℃, the Tr of low yield dairy was between 38.5 and 39℃; if the Ta was down to 24℃, Tr of high yield dairy was lower than 39℃.

第一阶段，舍外Ta平均达37.5℃时，A、B舍内Ta分别为35.5和36.7℃，此时Tr达到39.5℃以上，尤以A舍内高产奶牛22：00最高，平均达41℃；第一阶段平均RR在65次/min以上，高产奶牛在22：00、低产奶牛在15：00最高，分别为94和95.9次/min；平均mTs高产奶牛在15：00高达37.6℃；第三阶段，舍外平均Ta在15：00最高为27.6℃时，此阶段舍内Ta均高于舍外。

The simulation results of the cylinder-block model were given in the article, consistent with that of Middlemen"s. It was shown that the block part could improve the uniformity of the flow distribution. The analysis of the flow field of some materials with different power-law factor in the die designed by Mckelvey was close to that of Mckelvey"s. It was shown that the flow distribution was uniform in the die that can producesheets with 1200mm in width. During the analysis the flow channel was divided into several parts to get the details about the fluid flow in each area. A conclusion was drawn after analyzing the influence of the die pressure on the flow distribution, that the higher pressure is good for the output and bad for the uniformity of the distribution. Simulation of the die applying the cylindrical main runner showed the fact that when the cylinder replaced the cone the change of uniformity was trivial. The uniformity of the non-block flow channel was so poor that the block in the proper position of the die channel was necessary. The project of the die for the wider sheet was put forth, and the analysis of the fluid flow in the die was shown.

本文模拟了圆柱管缝隙流模型的三维流动场，得到了与Middlemen结论一致的流动均匀性，并通过模拟直观地展示了采用阻尼块可以改善挤出均匀性的现象；分析了多种幂率指数的流体在Mckelvey等人设计的流道模型中的流动分布，得到了与Mckelvey的分析结果接近的压力降和流量偏差；本文建立了1200mm幅宽机头流道的三维有限元模型，并逐个分析各个区域和多个截面的流动分布状况，由此揭示了1200mm幅宽L-型机头流道内的流北京化工大学硕士学位论文动分布基本均匀，并在分析了机头压力对于挤出流动分布的影响后得出结论：较高的机头压力可以获得较大的产量，但是不利于物料均匀分布；模拟了采用圆柱管主流道时机头流道内的流动分布，得出结论：主流道由圆锥管改为圆柱管时对流动均匀性影响不大，通过设置适当的阻尼仍然可以达到挤出平衡；模拟了两端进料式圆柱管无阻尼流道的流动均匀性后得到结论：两端进料式无阻尼结构的流动均匀性较差，宽机头中仍然有必要设置阻尼块；确定宽幅机头流道的基本方案，并设计了一种宽幅防水卷材挤出机头流道的结构，通过流动模拟表明该种结构的流动均匀性较好；考察了宽幅机头对于不同厚度片材的挤出适应性，得到结论：当生产不同厚度的片材时，阻尼块高度需要调整。

The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了