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ResultsThe optimum synthetic conditions were 2 g cinnamic acid,microwave power was 600 W,reaction temperature was 115℃,reaction time was 14 min,mass percent of macroporous resin was 30%,the molar ratio of cinnamic acid to isobutyl alcohol was 1:7, and the yield of the new process was 89.3%.

结果肉桂酸2 g，酸醇摩尔比1∶7，催化剂用量30%，微波功率600 W，微波温度115℃，微波时间14 min，转化率达89.3%。

The results showed that :(1) sex ratio of Macaca mulatta population in Neilingding Island was 1∶ 0.98 , in which infancy group was 1∶1.07 , juvenescent group was 1∶0.93 , middle age group was 1∶0.6, middle-old age group was 1∶0.57 , old age group was 1∶1.14 ;(2) age composition pattern of Macaca mulatta population in Neilingdi...

结果表明：( 1 )内伶仃岛猕猴种群雌雄性比为 1∶ 0 。98，各年龄组性比幼年组为 1∶ 1 。0 7，青年组为 1∶ 0 。93，中壮年组为 1∶ 0 。6 ，中老年组为 1∶ 0 。5 7，老年组为 1∶ 1 。1 4 ；( 2 )列出了猕猴种群年龄结构图，确定了年增长率为 6 。9%，种群数量的动态公式是 Nt=2 0 0 e0 。0 69t；( 3)预测了未来 1 0 a的猕猴种群的数量和发展，种群数量从 2 0 0 1年到 2 0 1 0年增长到 1 2 0 0头左右；( 4 )内伶仃岛猕猴种群发展的最适容纳量为 90 0～ 1 1 0 0头(平均 1 0 0 0头)。

Results 20 cases of CT examination had made proper diagnoses,the CT imaging finding about the most of peripheral cholangiocarcinoma included:There was the atactic low density tumour in liver,its borderline was unclear,enhanced scan appearances were irregular peripheral enhancement,centripetal enhancement,and delaying enhancement;There was concretion and dilatation of bile buct in or around the focus;The local envelope of liver was lacunose and the local liver was atrophied,There was abroad putrescence and liquescence in the focus.

结果 20例CT做出了正确的诊断，部分周围型胆管细胞癌的CT表现肝内不规则低密度肿块边界欠清，增强扫描表现病灶边缘呈不规则环状强化，有向心性强化的趋势，出现延迟强化；病灶内或周围可见结石，病灶内及周围肝内胆管扩张；局部肝包膜回缩、肝叶萎缩，病灶内大片坏死、液化。

For example, the depth of mountain tundra soil profile was 75 cm, and apparent 14C ages was 1315 a; the depth of brown coniferous forest soil profile was 40 cm, and apparent 14C ages was 870a; the depth of lessive black brown soil was 120 cm, and apparent 14C ages was 5145 a.

这不仅是由于它们的生化性质及其化学组成不同而造成的，而且还因为土壤有机质分解与累积相互影响制约的结果。

T included a land tax which the prime minister .loyd george ,had put it on purpose, knowing that the majority of land-owning lords would resist it.it was a clever trap,and they fell straight into it .for centuries, custom had allowed them to refuse an ordinary bill;but this time they were refusing a budget because it was against their own interests.it was time that their power was reduced.the next year Edward VII died,and a new election showed that the people supported the government,Edward's son,george v,had to make the lords change their minds.he threatened to make now liberal lords,just as william iv had done to pass lord grey's reform bill.the country was relieved to see that the royal power could still be used to support its will,and the position of the crown was strengthened.

这是一个绝妙的陷阱，他们跳了进去。几百年来，习惯上他们可以拒绝通过普通的法案，但这一次他们拒绝的是政府预算，因为经有悖于他们切身利益。这正是削弱他们势力的时机。次年，爱德华七世去世，新一轮选举表明人民支持政府。爱德华的儿子乔治五世不得不说服上议院改变主意，他威胁上议院，他要加封自由党新贵族，正如威廉四世为通过《格雷议会改革法案》时所采取的措施那样。全国人民欣慰地看到皇室权势还能用来支持他们的意愿，皇室的地位得到了加强。

The percentage of TGF-β〓 positive cell in the pancreatic cancer tissue (43.8±5.2)％ was significantly higher than that in adjacent pancreatic tissue (28.7±3.6)％, P＜0.05. The worse the cancer cells differentiated and lymphy node metastasis, the more over-expression of TGF-β〓. 2. The percentage of Tr positive cell in the pancreatic cancer tissue (41±4)％ was significantly higher than that in adjacent pancreatic tissue (23±3)％, P＜0.05. The worse the cancer cells differentiated and lymphy node metastasis, the more over-expression of Tr, but the expression of Tr protein was not correlated with lymphy node metastasis (P＞0.05). 3. The percentage of β-GCD positive cell in the pancreatic cancer tissue (62.5± 4.1)％ was significantly higher than that in adjacent pancreatic tissue (33.5±2.8)％, P＜0.05. The worse the cancer cells differentiated and lymphy node metastasis, the more over-expression of β-GCD in pancreatic cancer tissue, but the expression of β-GCD protein was not correlated with lymphy node metastasis. P＞0.05. 4. the expression of β-GCD was significantly correlated with TGF-β〓 and Tr in the pancreatic cancer tissue.

结果如下：1、胰腺癌组织TGF-β〓阳性细胞百分率(43.8±5.2)％明显高于癌旁胰腺组织(28.7±3.6)％，P＜0.05；且癌细胞分化愈差或有淋巴结转移TGF-β〓过度表达愈多。2、胰腺癌组织Tr阳性细胞百分率(41±4)％，明显高于癌旁胰腺组织(23±3)％，P＜0.05；且不同分化程度胰腺癌组织Tr表达强度不同，分化程度愈低，表达强度愈高，P＜0.05；但胰腺癌Tr表达强度与淋巴结是否转移无关，P＞0.05.3、对于胰腺癌组织TGF-β〓和Tr表达，检测胰腺癌组织(32例)β-GCD阳性细胞百分率分别为(62.5±4.1)％或(62±4)％，分别明显高于癌旁胰腺组织β-GCD阳性细胞百分率(33.5±2.8)％或(43±3)％，P＜0.05；不同分化程度胰腺癌组织β-GCD表达强度不同，分化程度越低，表达强度越高，P＜0.05；但胰腺癌组织β-GCD表达强度与淋巴结是否转移无关，P＞0.05.4、TGF-β〓、Tr和β-GCD在胰腺癌组织中的表达随着分化程度的改变，呈现一致性的关系，而且TGF-β〓与淋巴结转移有关，Tr和β-GCD与淋巴结是否转移无关。

The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

RESULTS: Success rate of open osteotomy model establishment was 90%. Average modeling time was 17 minutes. Rate of malunion and nonunion was 40%. Successful rate of each closed fracture models was 85%, with average modeling time was 12 minutes. There was no malunion and nonunion.

结果：开放截骨模型每只造模成功率90%，平均造摸时间为17min，骨折畸形愈合及不愈合率40%；闭合骨折模型每只造模成功率85%，平均造模时间12min，未见畸形愈合及不愈合。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

Results When the culture medium was supplemented with 0 .05μg/ml sodium selenite,the proliferation of transformed cells was significant ly promoted and the transformation did not change remarkably,when supplemented w ith 0.25μg/ml,the proliferation was promoted but the transformation was inh ibited,and when with 0.75μg/ml and 1.50μg/ml,the proliferation was not pro moted and the transformation was strongly inhibited.Big dose of (1.50μg/ml) sodium selenite m ade the nuclei of the untransformed lymphocytes gather together like a few grape s doing,and even caused them to fuse.

结果 在培养物中添加0.05μg/ml亚硒酸钠能显著促进转化细胞增殖；添加0.25μg/ml可促进转化细胞增殖，但显著抑制细胞转化；添加0.75μg/ml和1.50μg/ml，不仅不能促进细胞增殖，而且强烈抑制细胞转化。1.50μg/ml剂量的亚硒酸钠使非转化淋巴细胞发生葡萄状聚集甚至核融合。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。