查询词典 was

Isolations of the s.aureus insoluble peptidoglycan:(1) the s.aureus insoluble peptidoglycan was gained by cold, hot and hypersound affection repetat of s.aureus(ATCC25923);(2) the s.aureus insoluble peptidoglycan was gained by 10% trichloracetic acid, 5% trichloracetic acid,NH4HCO3 buffer with trypsin digestion of s.aureus(ATCC25923) in order. 2. Isolations of the s.aureus soluble peptidoglycan and quality control:(1) the s.aureus soluble peptidoglycan was released by short dose benzylpenicillin sodium and purified by sephadex G-100.(2) The quality control in the process of isolation: the soluble peptidoglycan was detected by SLP reagent kit; the LPS was detected by kinetic turbidimetric limulus test;and the protein was detected by improved LOWRY reagent kit.

金黄色葡萄球菌可溶性肽聚糖的分离提取及其质量控制(1)采用青霉素钠诱导法和葡聚糖G-100(Sephadex G-100)凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖；(2)金黄色葡萄球菌可溶性肽聚糖提取过程中的质量控制：采用SLP试剂盒对提取物定性检测，应用动态浊度法鲎试验测定提取物中内毒素含量，采用改良LOWRY试剂检测提取物中蛋白的含量；(3)采用青霉素诱导和Sephadex G-100凝胶柱分离提取金黄色葡萄球菌可溶性肽聚糖的条件：青霉素使用量、加入青霉素后孵育时间和菌液浓度等优化选择。

The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 130dB infrasound treatment In 1 day group, Bcl-2mRNA and P53mRNA were expressed lightly in CA1-CA3 areas in hippocampus and dentate gyrus. Blood vessels dilated lightly. In 7d group, Bcl-2mRNA and P53mRNA were more expressed in areas mentioned above. Dilation of capillaries and small vessels was more obvious. Bcl-2mRNA and P53mRNA were strong positive in endothelial cells of vessels. In 14d group, Bcl-2mRNA and P53mRNA expression was obviously increased in pyramidal cells of hippocampus and granular cells of dentate gyrus, showing deep blue staining. a The endothelial cells in some vessels were also strong positive for Bcl-2mRNA and P53mRNA. Dilation of capillaries and small vessels was lessened. In 21d group, Bcl-2mRNA and P53mRNA expression was decreased in pyramidal cells. In 28d group, little change about the expression and distribution of Bcl-2mRNA and P53mRNA was observed. Lots of Bcl-2mRNA and P53mRNA were scattered in cortex of temporal lobe around hippocampus and callosal gyrus area. Dilation of capillaries and small vessels was lessened obviously. The expression of Bcl-2mRNA and P53mRNA in hippocampus after 16Hz, 90dB infrasound treatment.

次声作用后Bcl-2mRNA和P53mRNA在海马的表达，16Hz、130dB次声作用1d组：两者在海马〓区和齿状回轻度表达，海马内血管轻度扩张；7d组：两者表达在上述区域较1d增多，微血管和小血管扩张较显，血管内皮细胞内可见呈强阳性表达的Bcl-2mRNA和P53mRNA；14d组：海马锥体细胞和齿状回颗粒细胞内Bcl-2mRNA和P53mRNA阳性表达明显增多，呈深兰色，在部分血管内皮细胞内两者强阳性表达，微小血管扩张状态较7d减轻；21d组：锥体细胞胞浆内Bcl-2mRNA和P53mRNA表达减弱；28d组：上述表达及分布与21d组相比无明显改变，海马周围颞叶皮层和扣带回区域亦可见大量散在分布的Bcl-2mRNA和P53mRNA表达，血管扩张状态已明显减轻。90dB次声作用1d-7d后，较对照组无明显变化，14d-21d后轻度表达，28d后，Bcl-2mRNA和P53mRNA表达较强。

Result The optimal condition was as follow: Raw materials was 0.500 g Chimonanthus praecox Link. dry powder, the extraction agent was 70% alcohol solution, the ratio of raw material to extraction agent was 1:60, the microwave power was 288W, and the extraction time was 450 s for three times.

结果］原料0.500 g的腊梅花粉末，以70%的乙醇溶液作提取剂，提取剂的用量为1：60，微波功率为288 W，在提取时间为450 s下提取次数3为最佳工艺。

Results The IC50 of SPG-Rg3 was (155.7±0.71) mg·L-1,when the concentration of SPG-Rg3 was arranged from 37.5 to 600.0 mg·L-1,the growth inhibitory rate of MCF-7 cells was higher following the increase of its concentration,the cell growth was greatly inhibited compared with control group(P.05); cell cycle was changed,the cell number of S period increased compared with control group(P.01),the cell number of G2/M period decreased compared with control group(P.01),in experiment group there was an apoptotic apex before G1 apex,and the number of apoptotic cells increased; most of cell nuleus in SPG-Rg3 (150 mg·L-1)experiment group appeared yellow or chrysoidine fluorescence,contracted,beaded and crescent; the result of immunocellularchemical staining of caspase-8 indicated that caspase-8 protein had no expression in control group,but stronger expression in SPG-Rg3 group.

结果：SPG-Rg3 IC50为（155.70±0.71） mg·L-1，当SPG-Rg3浓度在37.5~600.0mg·L-1时，MCF-7细胞的生长抑制率随SPG-Rg3浓度的增加而增大，与对照组比较，细胞增殖受到明显抑制（P.05）；MCF-7细胞的生长周期也发生变化，S期细胞数增加，明显高于对照组（P.01，G2/M期细胞数则明显少于对照组（P.01；并且在G1峰前出现明显的凋亡峰，凋亡细胞数增加。

The fundamental imaging features of osteosarcoma were showed bone destruction, periosteal reaction, neoplastic bone and soft-tissue mass. Among 49 cases examined by radiography, osteoblastic pattern sarcoma was found in 19 cases, osteolytic pattern sarcoma was found in 13 cases and mixed pattern sarcoma was found in 17 cases. The detection rates of bone destruction, periosteal reaction, soft-tissue mass, neoplastic bone and Codman triangle by radiography were respectively 61.2%, 95.9%, 71.4%, 73.5%and 27.7%. All 10 cases with CT scan were showed bone destruction, soft - tissue mass and neoplastic bone. The detection rates of periosteal reaction,憇ieve sign挶 and Codman triangle with CT scan were respectively 90%, 90% and 11%. The tiny neoplastic bone and"sieve sign"were showed sensitively by CT, but Codman triangle was showed by radiography superior to CT.32 cases with MRI scan presented the normal marrow was replaced by the tumor, which was showed low to intermediate signal intensity on T1-weighted images and promiscuity signal intensity on T2-weighted images. 7 cases presented hemorrhage and 24 cases presented necrosis.

骨肉瘤基本影像表现为骨质破坏、骨膜反应、瘤骨和软组织肿块。49例X线表现为成骨型19例，溶骨型13例，混合型17例；X线对骨质破坏、骨膜反应、软组织肿块、瘤骨和Codman三角的检出率分别为61.2%、95.9%、71.4%、73.5%和27.7%；10例CT表现均可见骨质破坏、软组织肿块及瘤骨，CT对骨膜反应、筛孔征和Codman三角的检出率为90%、90%和11%；CT对显示细小肿瘤骨和筛孔征最敏感，但对Codman三角的显示不如X线平片；32例MRI表现为骨髓腔内正常的骨髓高信号被肿瘤信号所取代，T1WI呈低～等信号，T2WI呈混杂信号，7例合并出血，24例中央坏死囊变，32例增强扫描均呈不均匀强化及瘤周可见水肿区。

Results (1)CIMT and FIMT in CHD group were significantly higer than those in non-CHD group(p＜0.05), The detection rate of plaques in CHD group was significantly higer than those in non-CHD group(p＜0.05);(2) The sensitivity of carotid and femoral atherosclerosis plaques in young CHD patients was 62% and 57%. The specificity was 90% and 86%. The positive predictive value of carotid and femoral atherosclerosis plaques in young CHD patients was 92% and 88%. The negative predictive value was 58% and 53%. The predictive value of combinant measurement of carotid and femoral atherosclerosis plaques was not improved.

结果 （1）冠心病组CIMT、FIMT与非冠心病组比较，前者均高于后者（p＜0.05）；冠心病组颈动脉、股动脉粥样硬化斑块检出率均明显高于非冠心病组（p＜0.05）；（2）颈动脉、股动脉粥样硬化斑块预测年轻冠心病的敏感性分别为62%、57%，特异性为90%、86%，阳性预测值分别为92%、88%，阴性预测值为58%、53%；颈动脉、股动脉联合检测未见明显提高预测值。

Health preservation of sexual activities in China has a very long history. It was recorded in Zhouyi and the Book of Songs in dramatic aestheticism and Zhou dynasty, and the modem researchers conject that eight diagrams and Taiji figure is products from reproductive worship; it was founded in the Spring and Autumn Period and the Warring States Period, and there were correlated theories in the works of various schools of thought and medicine bamboo slips in Han Tombs at Mawangdui, and noticeably, the yellow emperors internal classic established the basic theories of health preservation of sexual activities; it was flourishing in Han and Tang dynasty, which was a thriving and prosperous dynasty in the whole Chinese history and is quite open to sex, there were many kinds of correlative books appeared; it was blocked by the popularity of neo-confucianism of Cheng-Zhu; it was lingering in Ming and Qing dynasty because of the fast development of Chinese medicine and the persistent harm of feudalist ideology.

我国房事养生学历史悠久，其渊源于远古商周，早在《周易》、《诗经》中已有论述，当今学者即推测八卦太极图为生殖崇拜之产物；奠基于春秋战国，诸子百家的著作中有相关理论，马王堆汉墓医书中有大量的房事养生论述，而《内经》奠定其理论基础；繁荣于汉唐时期，正值中华民族繁盛之时，其包容开放的态度使有关房事养生的著作层出不穷；阻滞于宋元两朝，因程朱理学思想的盛行而严重阻碍了房事养生学的发展；徘徊于明清时代，明清时代中医学得到一度的飞速发展，也稍微带动房事养生学的进步，但封建理学思想的遗毒仍贻害无穷，故徘徊不前。

Based on the settings and geological and geochemical characteristics of granitoids of different orogenic belts (exemplified by orogenic granitoids in Altay, East Kunlun, Yanshan in North China, Northeast China and Nanling in South China), the relationships between granitoids and continental crustal growth are discussed, and five modes of continental crustal growth in the continent of China are proposed;they are as follows:(1)the Altay mode of continental growth is that the material and heat of the Paleozoic convertive mantle formed on the background of the Paleo-Asian Ocean were input into the Paleozoic continent and mixed with the materials of the upper crust;(2) the East Kunlun mode is that the basement of the Proterozoic orogenic belt was reworked by the heat input and materials of the Paleozoic-early Mesozoic convective mantle formed on the background of TTG continental crust of the Proterozoic orogenic belt;(3) the Northeast China style is that the Phanerozoic continental crust was reworked by the heat input and materials from the convective mantle formed on the background of the Yanshanian Central Asian orogenic belt;(4) the Yanshan mode is that the Archean basement was reworked by the heat input and materials from the Yanshanian convective mantle;and (5) the Nanling mode is that in South China what was input by the Yanshanian convective mantle into the continent was mainly heat and subordinately materials and the crustal material recycle was the dominant mode for the continental growth (showing zero continental growth).

根据不同造山带花岗岩的形成背景，地质地球化学特征差异，以阿尔泰，东昆仑，华北燕山，东北和南岭造山带花岗岩为例讨论花岗岩与大陆地壳生长的关系，区分出中国大陆的5种大陆地壳生长方式：阿尔泰式是古亚洲洋背景上形成的古生代对流地幔物质，热输入和上地壳混合为主的方式；东昆仑式是元古代造山带TTG陆壳背景基础上古生代—早中生代对流地幔物质和热输入，改造元古宙造山带基底的方式；东北式是燕山期中亚造山带背景上对流地幔物质和热输入改造显生宙陆壳的生长方式；燕山式是燕山期对流地幔物质和热输入改造太古宙基底的方式；南岭式燕山期对流地幔输入大陆的是以热为主，物质为辅，大陆地壳生长是以陆壳物质再循环为主的生长方式。

In the course of establishing standard operating procedure, The temperature, time , the feasibility without decorticating and efficiency were investigated when alliinase was deactivated used a dry heating apparatus in tunnel type, but the efficiency can be achieved less than 70%, need to be improved; The reformative cribriform plate has been proved to be good in practice result in pulping enzyme-deactivated garlic without dehulling; The extraction efficiency achieved more than 90% when volumic ratio of 20% alcohol and water is 3 I 1 and 2:1 was adopted to extract alliin two times respectively; During exact filtration, the ratio of diatomite in precoating and filtration was 3% and 1% respectively, the losses of alliin adsorbed by diatomite in extraction solution were not remarkable. In the stage of alliin isolation by ion exchange resin, three simple and sensitive detected measures were successfully adopted to control the whole process; the efficiency of alliin isolation was similar to laboratory results; The alliin yield was over 50% in acidic fractions and the purity quotient of alliin achieved above 50% after drying; Furthermore, the phenomenon was found that losses of alliin were seriously affected by the temperature and time periods in concentration.

在制定中试生产标准操作规程中，考察了隧道式干热烘干机杀酶的温度、时间和效率，干热灭酶效率只能达到70%，待改进；改良的筛板使带皮打浆效果良好，证明非脱皮熟蒜打浆的可行性，减少了工序，降低了成本；提取过程中，20%酒精分别1∶3和1∶2提取2次，效率达90%以上；硅藻土板框过滤时，探索了精滤过程中提取液中预涂和过滤时硅藻土的比例分别为3%和1%，并考察硅藻土对蒜氨酸的吸附损失，滤液澄明度良好，蒜氨酸损失很少；在树脂吸附分离阶段，采用三种简便灵敏检测手段控制生产过程，分离蒜氨酸的效果与实验室一致，酸性流分中蒜氨酸收率可达50%以上，干燥后蒜氨酸纯度达50%以上；试验发现浓缩蒜氨酸洗脱液时温度和浓缩时间对蒜氨酸损失的具有很大影响。

Results The DNA was obtained from PPJ through a nasopancreatic tube. Aberrant p16 methylation and K- ras gene mutation were detected in the same samples of PPJ. Sensitivity,specificity,positive predictive values,negative predictive values and accuracy of HE staining for pancreatic cancer were 40%,100%,100%,45.4% and 60.0%,respectively. Of the 20 cases of pancreatic cancer,K- ras gene mutation was detected in 14 (70%) and the p16 gene was shown to be methylated in 7 (35%). Of the 8 cases of chronic pancreatitis,K-ras gene mutation was detected in 2 (25%). Of the 2 cases of mucinous cystoadenoma of pancreas,K-ras gene mutation was detected in 1 (50%). Aberrant methylation of p16 was not detected in pancreatic juice samples from patients with chronic pancreatitis and mucinous cystoadenoma of pancreas.

结果 所有胰液标本均成功抽提出DNA，30例胰腺疾病病人胰液标本同时进行了K-ras基因突变和p16基因启动子区5′CpG岛甲基化检测，其中20例胰腺癌病人胰液中K-ras基因突变率为70%（14/20），p16基因甲基化率为35%（7/20），8例慢性胰腺炎中K-ras基因突变率为25%（2/8），2例胰腺囊腺瘤病人中K-ras基因突变率为50%（1/2），慢性胰腺炎和胰腺囊腺瘤病人胰液中无p16基因甲基化。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。