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Cell proliferation and viability were assayed 48h after transfection, and MDA-7 demonstrated selective inhibition of tumor cell growth, inhibitory rates of A549, Hela and HepG2 cell lines were 25%, 20% and 19%, respectively, but had no significant effect on human fetal kidney derived 293 cell line. Hela cell was screened by G418 for 2 weeks after pcDNA3-MDA-7 and monolayer colony was counted, its monolayer colony formation was 30% of cells transfected with pcDNA3. 0 vector. CMV-driven MDA-7 adenovirus vector was constructed. 293 showed no significant apoptosis during adenovirus packaging and the unpurified adenovirus titer was about 1×10〓pfu/ml. Cos 7, A549, Hela, HepG2 and Hep3B cell was infected with Ad-GFP at different MOI.

二。黑色素细胞分化相关蛋白-7（MDA-7）的克隆及功能研究：利用RT-PCR方法从5月龄人胚胎脾细胞扩增MDA-7的编码序列，经测序鉴定序列与文献报道一致后，与真核表达载体pcDNA3.0连接，构建pcDNA3-MDA-7表达载体，瞬时转染293、A549、Hela和HepG2细胞后抽提细胞总RNA，RT-PCR结果显示表达载体可介导MDA-7在不同细胞系中有效表达；转染48h后测定细胞增殖和活力，MDA-7可选择性抑制肿瘤细胞的增殖，对A549、Hela和HepG2细胞的抑制率分别为25％、20％和19％，但是对人胚肾来源的293细胞生长无明显影响。pcDNA3-MDA-7载体转染Hela细胞后，以G418筛选2周后计数单层细胞集落形成数，计数仅为转染pcDNA3.0空载体的细胞的30％左右。

There were times when I was desperate about everything I saw and everybody I met, when I lost my health and felt that my body was as good as dead (Romans 4:19), when I was anxious and exhausted, when I was utterly helpless and utterly lonely, when my spirit was suffering in utter darkness. But while all this was happening, you were transforming my spirit into something I had never dreamed of before.

在我对世上一切事物，对自己对他人都感到绝望的时候，在我失去健康甚至感到"身体如同已死"（《罗》4：19）的时候，在我心力交瘁斗志全无的时候，在我最软弱最孤单的时候，在我心灵处於黑暗中极度痛苦的时候，你竟引我灵命走向我从未梦想的高处。

The results showed that there was a negative relationship between H2O2 content and CAT activity, thus H2O2 content was low when CAT activity was high. There was a close relationship between H2O2 content and differentiation process. H2O2 could be an important signal transduction factor during bud differentiation. IAA oxidase activity was decreased firstly, and then increased, which affected IAA oxidase and degradation pathway and regulate ratio of cytokinin and auxin. With differentiation process continuing, PPO activity was increased, which could cause browning or vitrifaction of seedling, or even malformation.

研究结果表明，鹅掌楸组培再生过程中，过氧化氢酶活性与H2O2含量变化呈负相关，CAT活性较高时，H2O2含量则较低。H2O2含量与鹅掌楸组培苗分化进程密切相关，H2O2可能作为信号因子在芽分化过程中发挥重要作用；IAA氧化酶活性呈现先下降后增加的变化规律，从而影响IAA氧化降解途径，调控细胞分裂素与生长素含量的比值，影响鹅掌楸芽的再生；PPO活性随培养时间的增加而升高，引起组培苗的褐变或玻璃化，甚至生长畸形。

The results indicated that (1) the economy in Zhejiang Province was in the developing stage of unchanged scale wage;(2) the inputoutput elasticity coefficients was the most sensitive to labour element among the factors which had effect of economic growth in Zhejiang Province.The results showed that the coefficient was 0.538 5 in labour element while the coefficients were 0.247 3 in land element and 0.321 6 in fixed asset elements respectively;（3）the contribution of the input of fixed assets was 6823% and still the major impetus to economic growth during this time;(4)the land use was not intensive basically in Zhejiang Province,the input of land elements was replaced by that of other elements during the study.So,labour intensive industry should be one of the industries in the future.

研究结果表明：(1)就规模效应而言，浙江省的经济增长处于规模报酬不变的发展阶段；(2)浙江省经济增长对劳动力要素的投入最为敏感，其中土地要素、劳动力要素和资本要素增加1个单位的投入量对经济增长的推动为 0.247 3、0.538 5 和 0.321 6；（3）固定资产投入在研究期间是浙江省经济增长的主要推动力，其贡献率达到6823%，而劳动力要素与土地要素则分别为2346%和625%；(4)浙江省土地利用不够集约，在研究期间土地要素对经济增长的影响能被资本要素与劳动力要素有效替代，而劳动力要素则难以被资本投入与土地投入有效替代，劳动集约型产业应该成为浙江省未来发展方向之一。

In this dissertation, InGaAs/InGaAsP/InP MQW microdisk laser and its characterization were presented. Some conclusions are as following: the characteristics of lasing threshold was discussed by using rate equation. The dependent relations between threshold and cavity volume, spontaneous emission coupling efficiency β were investigated. The whispering gallery mode and spontaneous emission in a microdisk geometry were then studied. Almost all spontaneous emission was coupled into the lowest order TE mode when the normalized thickness of photonics well was between 0.15 and 0.5. The design principle and device process of InGaAs/InGaAsP/InP MQW microdisk laser were presented. The microdisk lasers with the diameter of 8μm, 4. 5μm and 2μm were succesfully fabricated by using etching techniques. The pulse lasing thresholds for the microdisk with the diameter of 8μm and 4. 5μm were 170μW and 15μW, a record results compared with the published in the literature. The cw lasing threshold for a 2-μm-diameter microdisk was only a few μW. We haven't found similar report up to now. To solve the problem of directional output in a microdisk laser, the power coupling efficiency in a double disk geometry was investigated with the theory of waveguide mode couple. A new structure of electrically pumped microdisk laser with output waveguide was designed, making a foundation for the practical use of microdisk laser.

本文主要研究InGaAs/InGaAsP/InP多量子阱半导体碟型激光器的研制和特性表征，并取得如下结果：利用速率方程理论讨论了激光器激射阈值的特性，分析了阈值与腔体积和自发发射耦合系数β之间的关系；讨论了微碟光子阱结构中的自发发射特性和微碟中WGM模式特性；光子阱结构在其归一化厚度在0.15-0.5之间时，几乎全部自发发射进入到最低阶TE模式中；研究了InGaAs/InGaAsP/InP多量子阱微碟激光器的结构设计原理和器件的制备工艺；利用刻蚀方法成功制备出直径分别为8μm、4.5μm、2μm的碟型微腔激光器；直径8μm和4.5μm的微碟脉冲光泵浦激射阈值分别为170μW、15μW，是目前所见文献报导中比较低的；直径2μm的微碟连续光泵浦激射阈值仅几μW，目前尚未见到达到类似结果的研究报导；为解决微碟激光器激射光定向引出的问题，通过波导耦合模理论分析了双层碟之间功率耦合效率；据此设计了新型带耦合输出波导的电泵浦微腔激光器，为进一步研制可实用的电泵浦微腔激光器打下基础。

The results showed that the higher thedischarge voltage or charge capacitor was, the higher the temperature of the plasma was,and the longer plasma discharge time was. There was a late discharge when the inputenergy reached the critical value. The greater the capacitance or voltage was, the earlier thelate discharge of the plasma was. The temperature changes of plasma at the beginning of alate discharge were not significant under different discharge conditions. Each SCB had itstemperature range respectively.

结果表明：放电电压或充电电容越大，等离子体温度越高，等离子体的放电时间也越长；对于不同的SCB，当输入能量超过一定的临界值后，将产生等离子体后期放电；电压或电容越大，后期放电开始的时间越早，而后期放电开始时的等离子体温度，在不同的放电条件下变化并不大，不同的SCB均有其各自的温度变化范围。

About two weeks ago when I was head over heels in love with Neruda's poetry, I came across this poem. It was written for his object of affection, Albertina Rosa Azócar in his youth. They met when studying French in Santiago's Pedagogical Institute at the age of 16 or 17. Soon the morose and quiet Albertina sparked passionate flames in the young boy. She was his muse for many lyrical texts and poems for more than 10 years. The most famous ones would be Veinte Poemas de Amor y Une Cancion Desesperada, Twenty Love Poems and a Desperate Song . Even in 1927, after he traveled to countries like Burma, Ceylon, Java and Singapore to work in dark consular positions, he kept writing to Albertina in postcards, hotel letter paper, napkins, loose-leaf pages. She didn't respond to his love with as much passion. In 1930, he began to form a friendship with Mary Antoinette Haagenar from Holland when transferred to Java. Despite her coldness and lack of interest in poetry, he decided to marry her in December of the same year out of solitude in the remote island. It was not known how Albertina felt about Neruda's marriage, but she was married to ngel Cruchaga Santamaría, who was also a poet, five years later.

前几周当我不可自拔地沉迷於聂鲁达的诗作之际，偶然发现了这首诗，这是诗人写给年少时爱恋的对象阿贝蒂娜(Albertina Rosa Azócar)，他们在十六七岁就读於圣地牙哥师院的法文系时相识，之后聂鲁达便展开了长达十多年的爱恋，他为阿贝蒂娜写下许多情诗、文章和信件，其中包括最有名的「二十首情诗和一首悲歌」，甚至在1927年之后，他去到许多亚洲国家如缅甸、锡兰、爪哇和新加坡担任秘密外交特使，他把对阿贝蒂娜的思念写在明信片、餐巾纸、活页纸、旅馆信纸上，但是内向沉静的阿贝蒂娜并未回以同等的热情，於是在1930年，身处异乡的聂鲁达遇见来自荷兰的玛莉哈格纳，虽然她是冰山美女，也无法分享聂鲁达对诗的喜爱，他们在同年12月结婚，阿贝蒂娜对聂鲁达婚事的反应没有任何记载，不过五年之后她也迈入婚姻，嫁给同为诗人的圣塔马利亚(ngel Cruchaga Santamaría)。

Results The UV-B treatment caused the declines of chlorophyll content, soluble protein content and water content of leaves, besides, dose-effect was existed. The decline of 30 μW/cm^2 treatment was bigger than that of 15 μW/cm^2 treatment. The influences of UV-B radiation on carotenoid content and anthocyanidin content were similar; the change trends were declined firstly then increased. The UV-B treatment with two doses restrained the electron transport of PSⅡ, particularly; the inhibitory effect was biggest after treated 2h, and then this effect was declined in 4, 6 and 8 h, so the dose-effect was existed. The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time. The high dose treatment caused huge damage to membrane system, while the result of low dose treatment was not obvious.

结果］UV-B处理导致叶绿素、可溶性蛋白含量以及含水量都降低，且存在剂量效应，30μW/平方公分处理的降幅大于15μW/平方公分；UV-B辐射处理对类胡萝卜素含量的影响与花青素相同，均表现为先下降后上升的趋势，2种剂量间差异不大；2种剂量的UV-B处理均抑制了PSⅡ的电子传递，尤其是处理2 h的抑制作用最大，4、6和8 h的抑制作用有所缓解，且存在剂量效应；2种剂量的UV-B辐射均抑制了叶片的光合速率，随着照射时间的延长抑制效应加大；高剂量UV-B处理对膜系统的损伤较大，低剂量UV-B处理结果不明显。

Though sometimes he was blindfolded ,no man nor god,zeus himself included,was safe from his evils.at one time the little naughty god was wounded by his own arrows and suchburning love was awakened in him for the human maiden psyche that he disregarded the constant interference of his mother and plucked up his courage to beg zeus for justice.another famousstory where eros played an important part was the argonautic expedition.medea,daughter of king aeetes,was wounded by eros'''' arrows,took jason''''s part in recovering the golden fleece and eventually became the hero''''s wife.

尽管有时他被蒙着眼睛，但没有任何人或神，包括宙斯在内，能逃避他的恶作剧。有一次这位淘气的精灵被自己的箭射中。对人间少女普赛克炽热的爱在他心中复苏，以致于他不顾他母亲的干预，鼓起勇气让宙斯给予公正评判。厄洛斯起了重大作用的另一个著名的故事是亚尔古英雄的远征。美狄亚，国王埃厄忒斯的女儿，被厄洛斯的神箭射中，和伊阿宋一起寻觅金羊毛，最后成为这位英雄的妻子。

Though sometimes he was blindfolded ,no man nor god,zeus himself included,was safe from his evils.at one time the little naughty god was wounded by his own arrows and suchburning love was awakened in him for the human maiden psyche that he disregarded the constant interference of his mother and plucked up his courage to beg zeus for justice.another famousstory where eros played an important part was the argonautic expedition.medea,daughter of king aeetes,was wounded by eros'' arrows,took jason''s part in recovering the golden fleece and eventually became the hero''s wife.

尽管有时他被蒙着眼睛，但没有任何人或神，包括宙斯在内，能逃避他的恶作剧。有一次这位淘气的精灵被自己的箭射中。对人间少女普赛克炽热的爱在他心中复苏，以致于他不顾他母亲的干预，鼓起勇气让宙斯给予公正评判。厄洛斯起了重大作用的另一个著名的故事是亚尔古英雄的远征。美狄亚，国王埃厄忒斯的女儿，被厄洛斯的神箭射中，和伊阿宋一起寻觅金羊毛，最后成为这位英雄的妻子。此主题出自 king。的博客

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。