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It was found that Pd-Co/active carbon catalyst was the optimal catalyst, and Pd-Co/γ-Al2O3 was also good, but silica and kieselguhr were not proper carriers for the object reaction, because of their stronger surface acidity, which could result in the formation of polymer, jammed the micro pores of catalyst, and then resulted in its deactivation. Addition of Bi, Ce and Cu promoters could not improve the activity of catalyst, but La and Ba promoter could enhance the activity of catalyst apparently, and the Co was the best promoter, especially Co was impregnate in advance. It was attributed to that the pre-impregnated Co may occupy the micro pores of active carbon, and cover some bad functional groups on the surface of catalyst, so that the active and selectivity of catalyst could be enhanced obviously.

研究结果表明，Pd-Co/活性炭催化剂是目标转移加氢反应的最适宜催化剂，Pd-Co/γ-Al203效果也较好，而硅胶和硅藻土由于表面酸性较强，容易造成生成聚合物而堵塞催化剂微孔、使催化剂迅速失活；此外，添加Bi、Ce、Cu等助剂时对活性并无改善作用，而添加La和Ba助剂时可使Pd的催化活性有所提高，效果最好的是Co助剂，尤其是先浸渍Co的Pd-Co/C催化剂，原因是Co占据了活性炭的微孔、覆盖了某些对目标反应不利的官能团，使其活性和选择性都有较大幅度的提高。

I was sheriff of this county when I was twenty-five. Hard to believe. Grandfather was a lawman. Father too. Me and him was sheriff at the same time, him in Plano and me here. I think he was pretty proud of that. I know I was.

25岁的时候，我是这里的警长，真难相信，爷爷曾是名执法人员，父亲也是，我和他曾在同一时期当过警长，他管Plano，我管这边，我想他一定很骄傲，我知道我也是。

Firstly, several kinds of schemes were proposed according to the design demand. The best scheme was chosen after analyzing and comparing the schemes. The robot's structure was designed with Pro/Engineer and AutoCAD software. Secondly, the kinematics analysis conducted, coordinate transformation matrix using D-H method was set up, and the kinematics equation direct solution and inverse solution was deduced. The manipulative interface about the kinematics equation direct solution and inverse solution was completed with VC++ and the velocity Jacobian of displacement matrix was constructed using differential transform method. In the process of the trajectory planning based on robot's kinematics analysis, I propone a method by which we can get middle nodal point with normalizing factor in order to simplify our searching for these middle nodal points. In addition, I give these middle nodal points with actual physics signification. For eliminating contradiction between real-time and accuracy, I bring forward separately limit of error and reversal interpolation method. For decreasing calculation quantity, we resort to tri-spline interpolation in the articulation space.We analyse the work range of the robot by resorting to graphic means.

首先，作者针对机器人的设计要求提出了多个方案，对其进行分析比较后，选择其中最优的方案后用Pro/Engineer和AutoCAD软件进行了机器人模型结构设计；其次，进行了运动学分析，用D-H方法建立了坐标变换矩阵，推算了运动方程的正、逆解，运用VC++制作了正、逆运动学求解的求解界面；并且用微分变换法推导了速度雅可比矩阵；在基于机器人的运动学的轨迹规划中，通过在操作空间的规划，提出了归一化因子来求解中间结点，通过它可以使求解中间结点变得更简单，并且赋予这些中间结点实际的物理含义，对于规划中精确性和实时性的矛盾，提出了以误差极限法和反向插值法来解决的方法；为了减少规划过程中计算量，在关节空间进行三次样条插值；然后借助图解法进行工作空间分析，作出了实际工作空间的轴剖图。

Results 20 cases of CT examination had made proper diagnoses,the CT imaging finding about the most of peripheral cholangiocarcinoma included:There was the atactic low density tumour in liver,its borderline was unclear,enhanced scan appearances were irregular peripheral enhancement,centripetal enhancement,and delaying enhancement;There was concretion and dilatation of bile buct in or around the focus;The local envelope of liver was lacunose and the local liver was atrophied,There was abroad putrescence and liquescence in the focus.

结果 20例CT做出了正确的诊断，大部分周围型胆管细胞癌的CT表现为肝内不规则低密度肿块边界欠清，增强扫描表现病灶边缘呈不规则环状强化，有向心性强化的趋势，出现延迟强化；病灶内或周围可见结石，病灶内及周围肝内胆管扩张；局部肝包膜回缩、肝叶萎缩，病灶内大片坏死、液化。

B Of the six basic media MS, MS1/2 (half-strength of MS salts and vitamins), WPM, DKW, B5 and SH, MS1/2 was the most proper one to induce somatic embryos. Somatic embryos generally regenerated directly from excised zygotic cotyledons. PGRs combination affected somatic embryogenesis significantly. Medium with NAA 1mg/L, TDZ 0.05mg/L, IBA 2—10mg/L combined with BA 10mg/L, or IBA 10mg/L integrated with BA 0-2mg/L gave the highest induction rate. Excised zygotic hypocotyls had the strongest potential to produce callus. Callus induction was also affected significantly by media and PGRs. The proper callus induction condition was MS1/2 medium containing NAA 1mg/L, IBA 10mg/L, BA 2-5mg/L and TDZ 0.05mg/L. Harvest period affect somatic embryogenesis significantly. Zygotic embryo explants collected from the end of July to the middle of August had strong potential to generate somatic embryos, when endosperm finished solidification, different parts of the embryos were completely formed, the size of embryos occupied about 2/3 of the embryo sac. Provided with optimized conditions, direct somatic embryogenesis rate can attain to 33. 68%, and callus induction rate of hypocotyls was up to 90.7%. Cytological observation on megasporogenesis and zygotic embryogenesis of Manchurian ash showed that the ovary was twicarpellum, twilocular with two ovules each loculus. The ovule was tenuinucellar and anatropous, with one megasporcocyte. The development of embryo sac is of the Polygoum type.

体细胞胚胎发生研究的结果表明：(1)成熟过程中的合子胚是诱导水曲柳体细胞胚胎发生的最佳外植体材料；(2)在所试验到的MS、MS1/2（将MS的所有成分均减半）、WPM、DKW、B〓、SH等六种基本培养基中，MS1/2是最适合诱导水曲柳体细胞胚胎发生的基本培养基；(3)水曲柳的体细胞胚胎发生以直接发生为主，体细胞胚主要来自于从合子胚分离的完整子叶；(4)培养基中的激素组合对水曲柳的体细胞胚胎发生有显著影响，诱导直接体细胞胚发生较好的激素组合有NAA 1mg/L+IBA 2，5，10mg/L+BA 10mg/L+TDZ 0.05mg/L和NAA 1mg/L+IBA 10mg/L+BA 0，2mg/L+TDZ 0.05mg/L；(5)合子胚分离的下胚轴具有最强的愈伤组织诱导潜力，少数愈伤组织可以分化出体细胞胚；(6)愈伤组织的诱导也受培养基和激素配比的显著影响，最适宜诱导的培养条件为MS1/2+NAA 1mg/L+IBA 10mg/L+BA 2，5mg/L+TDZ0.05mg/L；(7)采种时间对体细胞胚胎发生有显著影响。7月末到8月中旬的合子胚具有较强的体细胞胚发生潜力，此时种子尚未成熟，胚乳已呈固态，种胚的各个部分已分化完全，种胚体积占胚腔的大约2/3；(8)在各自综合的最适条件下，完整子叶的体细胞胚诱导率可达33.68%，下胚轴的愈伤组织诱导率可达90.7%。

Results There was a linear trend between logarithmic X and Y, the equation of logarithmic curve was able to be fitted, There was a linear trend between logarithmic Y and X, the equation of exponential curve was able to be fitted, There was a linear trend between logarithmic X and logarithmic Y, the equation of power curve can be fitted. There is a parabola pattern between X and Y, the equation of parabola was able to be fitted.

结果 X取自然对数与Y作散点图有直线趋势，拟合对数曲线方程；Y取自然对数与X作散点图有直线趋势，拟合指数曲线方程；X取自然对数与Y取自然对数作散点图有直线趋势，拟合幂函数曲线方程；原始数据X和Y成抛物线关系，拟合抛物线方程。

Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构，除去了SV40启动子基因，获得了pGL3-Enhancer载体，将获得的IFN-β启动子基因连入载体中，构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21，并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性，照度计检测荧光素酶活性增强，从而验证了所构建的重组质粒的有效性。

Maculate protocorm was highest in the KC solid medium, being 71.9%, and their seedling growth was best and the diameter of the protocorm was(5.8±0.3) mm. The survival rate of D.nobile protocorm was highest in the B5 solid medium, being 65.0%, and their seedling growth was best in the KC solid medium and the diameter of the protocorm was (3.8±0.6) mm.

秋花独蒜兰原球茎在KC固体培养基上的成活率最高，为71.9%，且幼苗生长发育状况最好，原球茎直径为(5.8±0.3)mm；石斛兰原球茎在B5固体培养基上的成活率最高，为65.0%，在KC固体培养基上的生长发育状况最好，原球茎直径为(3.8±0.6)mm。

Results (1) CCD fluorescent microscope was superior to laser scanning confocal microscope in collecting the fluorescent image of DCF. CCD fluorescent microscope was chosen to be the system applied in this research with the consideration of operation, cost and et al. The mercury-arc lamp of fluorescent microscope was fit for the requirement of our study. The wavelength range of the light output was 460-490 nm and the power density was about 100mW/cm in the condition of our study. Organelle-cell fluorescence intensity ratio analysis was more suitable than other methods.

结果：(1)CCD荧光显微镜采集到的DCF荧光图像的质量优于共聚焦显微镜，同时综合考虑包括采图质量、光照射剂量的可控性、实验成本和操作简便性等诸多因素，最终确定了CCD荧光显微镜作为实验研究的工具；荧光军医进修学院硕士学位论文中文摘要显微镜的汞灯输出功率稳定，光斑均匀，经本实验所选取的光路系统后输出波长范围为460一490nm，功率密度约为1 00 mw/cm；细胞器一细胞荧光强度比值法对于荧光分布位置的确定优于其他方法。

The positive expression both of p14ARF and mtp53 protein in NSCLC tissues were significantly different from that in nonmal lung tissues and tissues adjacent to the cancer (P.01).The expression of p14ARF protein was correlated with pathological degree（P＜0.01）,but it was uncorrelated with pathological staging,lymphaden metabasis and vessel invasion(P＞0.05).The expression of mtp53 protein was correlated with clinical pathology staging,pathological degree and vessel invasion(P＜0.05 or P＜0.01),but it was uncorrelated with lymphaden metabasis(P＞0.05).(2) The coexpression of p14ARF and mtp53 was 36.7％,their consistency was 38.8％,but the expression of p14ARF protein were negatively correlated with the expression of mtp53 protein(r=-0.3553,P＜0.001).Conclusion The results suggest that the decrease of p14ARF and the increase of mtp53 protein may play an important role in the genesis and development of NSCLC.

结果 (1)在正常肺组织、癌旁组织和NSCLC中p14ARF蛋白的阳性表达率分别为93.3%、83.3%、55.1%，mtp53蛋白的阳性表达率分别为6.7%、44.4%、79.6%；p14ARF蛋白和mtp53蛋白在NSCLC中的表达与正常肺组织和癌旁组织比较均有显著性差异(P＜0.01)；p14ARF蛋白表达与临床病理分期、有无淋巴结转移、有无脉管浸润无关(P＞0.05)，而与病理分化程度有关（P＜0.01）；mtp53蛋白表达与临床病理分期、病理分化程度(P＜0.01)及有无脉管浸润(P＜0.05)有关，而与有无淋巴结转移无关(P＞0.05)；(2)在NSCLC中，pl4ARF与mtp53蛋白共表达为36.7％，一致性为38.8％，二者表达呈负相关(r=-0.3553，P＜0.001)。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。