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The fragment was connected to pcDNA3.0 carrier which was cut by the same two enzymes to form the plasmid which was transformed into Escherichia coli DH5a.Appropriate clone was cultured to extract plasmid.The extract was analysed to confirm that it was constructed correctively.

将该片段连接到同样被KpnⅠ和XhoⅠ双酶切的pcDNA3.0载体中，转化大肠杆菌DH5α感受态细菌，挑取阳性克隆进行质粒提取，进行酶切鉴定，并获得阳性克隆。

This scheme was composed of two parts: intra prediction and inter prediction. For the intra prediction part, 8×8 block DCT-HT transform-domain converse was used, and then intra mode decision was done in HT domain to obtain the optimum mode, so that good intra prediction was acquired. For the inter prediction part, Motion Vector got from Variable Length Decoding in MPEG-2 stream was reused as Prediction Motion Vector of H.264, then motion prediction mode and search region were reset, so that better prediction result was acquired.

该方案中，帧内预测使用了8×8块的DCT-HT的变换域转换，然后在HT域进行Intra模式判决得出最优模式，从而得到理想的帧内预测；帧间预测重用了MPEG-2码流中变字长解码得到的运动矢量MV，作为H.264的PMV，通过重新设定运动预测模式以及搜索范围，从而获得较好的预测效果。

And this strain degraded H〓S without lag time. The heterotrophic bacteria's generation period was short and its growth was rapid. The result of experiment indicated when the strain Hm-6 was applying to the packed tower, the startup was more quickly and the control was more convenient than the autotrophic bacterium. Applying to deodorize the H〓S, the better removalability could be got. At the same time, comparing with the system used at home of which the biofilm directly formed with activated sludge, it runs in the neutral condition, has lower corrusivity to the equipment and was liable to handle. And this system didn't need the acclimation to become biofilm, so it can save a rather long acclimation time.

异养菌虽然不以硫化物作为能源物质，但其世代周期短、生长迅速，运行管理比自养菌更为方便，具有周期短，启动迅速等优点，接种了菌株Hm-6泥炭生物填料塔，对低浓度H〓S有较高的去除效果，且没有延迟期，与国内通常采用的直接以活性污泥挂膜的方式相比，应用该菌的脱臭系统在中性条件下运行，对设备的腐蚀性较低、运行过程较易控制，具有很好的应用前景。

Beijing Institute of Technology, Beijing 100081,ChinaIn order to get the high output power, a RF excited diffusively cooled all-metal slab waveguide CO2 laser was reported, in which gas discharge region was constructed by two aluminum side walls and aluminum electrodes. The region dimension was 2 mm height×20 mm width×386 mm length, and the gas composition was CO2:N2:He:Xe=1:1:3:0.26. This technique replaced the old ceramic and metal sandwich waveguide technique.The optimum out power 127 W was achieved from the RF excited diffusively cooled all-metal slab waveguide CO2 laser with the longitudinal voltage uniformity distribution technique and scaling area technique. The electro-optic conversion efficiency was about 14%.

为了获得较高的激光输出功率，介绍了射频激励扩散冷却全金属板条波导CO2激光器，放电区域由左右两个铝合金壁和上下两个铝合金电极构成，放电区域高2 mm，宽20 mm，长386 mm，工作气体混合比CO2：N2：He：Xe=1：1：3：0.26，该技术代替了过去的金属陶瓷结构，结合纵向电压均匀分布技术和面增比技术，获得了127 W的激光输出功率，光电转换效率高达14%。

During the calculation the results got by quintic spline kernel was the best so it was chosen as the proper kernel for the SPH simulation. By comparison of the simulation results got by SPH and finite volume method, it was proved that the buffer cells method proposed in the SPH simulation to implement the Dirichlet boundary conditions was accurate, and this is of great importance for the simulation of flows pass a pipe. At last, the SPH method was improved to simulate the liquid-solid two phase flows and the results show that this method was appropriate for the simulation of flows containing viscous fluid and macroscopic solid components. Now the two methods are both able to predict the continuum flows and rarefied flows severally, but the hybrid program still has some aspects to be improved.

首先用标准算例验证了SPH方法的理论模型和计算程序的准确性；通过比较确定选用五次样条核作为计算低雷诺数下的粘性连续流动时的核函数；采用SPH方法和有限体积法分别对同一管道流动进行了对比计算，证明了本文提出的边界缓冲区方法在实现SPH方法中的流体边界条件时的有效性，解决了SPH方法模拟管道流动时的一个关键问题；巧妙地使用SPH方法对化工分离流动中常见的固液两相流及固液耦合作用进行了模拟尝试，获得了较好的结果；在跨尺度计算方面，目前两个子程序已经能够分别模拟连续的和稀薄的二维管流问题，二者的耦合计算程序还处于调试和优化阶段，各个参数对于计算精度和计算速度的影响规律仍在探索中。

The influence of each condition on the reaction speed and yield was studied, and the optimal conditions were: the ratio of the amount of substance of aromatic amine and heptafluoro-isopropyl iodine was 1:1.1~1.5, the amount of substance of sodium dithionite, sodium bicarbonate, aniline compounds were respectively 1.1~1.3, the amount of substance of phase transfer catalyst TBAB and aniline compounds was 0.05~0.1, the concentration of aniline compound was 0.5~1.0 mol/L, reaction temperature was 10~20 ℃, and the solvent was aether.

最佳结果为：芳胺与七氟异丙基碘的物质的量比1：1.1~1.5，连二亚硫酸钠、碳酸氢钠与苯胺的物质的量分别为1.1~1.3，相转移催化剂四丁基溴化铵与苯胺的物质的量比在0.05~0.1，溶剂总用量使苯胺浓度在0.5~1.0 mol/L；反应温度10~20℃；溶剂采用乙醚。取代基供电子性越强，反应时间越短，产率越高；氨基的对位优先反应，氨基的邻位较难发生反应；且七氟异丙基总是优先取代在芳环电子云密度较高的位置上。

The main research work of this paper are focused on following areas:(1) Based on review of the theory and methods on measurements of particle fields, a new idea for obtaining particle size and velocity distribution within a spray through imaging the particle field with a laser light sheet was put forward;(2) A DPIV (Digital Particle Image Velocimetry) system, is fit for velocity measurements of low speed flows, was developed and expended to particle size distribution measurement;(3) An arithmetic for particle velocity field reconstruction was developed, and the velocity distribution of water mist was also obtained;(4) A software system for particle analysis, which based on image geometry emend, de-noise and image partition was developed, the parameters such as particle size distribution, mean diameter, number of particles, minimum and maximum diameter can be got with this system;(5) A water mist system was developed and its characteristics, such as droplet velocity, size distribution, number of droplets and spray cone angle under different conditions were obtained from experiments with PIVS;(6) The measurement results of water mist characteristics with PIVS were compared and analyzed with the simply simulated results, and in addition, in order to verify the accuracy of PIVS, some experiments were conducted with the standard particles, such as glass-ball with known mean diameter of 50μm and 115μm, metallic coated tracing particle with mean diameter of 12μm;(7) Some experimental studies on interaction of water mist with liquid pool fires were conducted.

本论文的主要工作包括以下几个方面：（1）在对粒子场测量的相关理论和具体方法进行综述分析的基础上，提出了通过采用激光片光对粒子场进行成像以获取其粒径和速度等参数分布的新思路；（2）研制了适宜于低速流动速度场测量的DPIV(Digital Particle Image Velocimetry)系统，并使其实现了对粒子场粒径分布的测量功能；（3）研制了基于粒子运动轨迹的速度场重建算法，获取了细水雾雾场的速度分布；（4）研制了基于几何校正、去噪、图象分割等图象处理方法的"粒度分析软件系统"，该系统既可分析给出粒子场的粒径分布直方图和平均粒径，还可给出粒子的数目以及最大、最小粒径等信息；（5）建立了一细水雾发生系统，并应用上述方法对不同压力条件下细水雾系统的雾场特性（如速度分布、雾滴粒径分布、雾滴的数目、喷雾张角以及雾化长度等）进行了实验测量研究；（6）对细水雾特性参数的PIVS测量结果与计算机简单模拟计算结果进行了定性比较分析，并利用平均粒径为50μm和115μm的玻璃球以及12μm的标准示踪粒子对PIVS系统的粒径和速度测量结果进行了实验验证，同时对其局限性进行了分析讨论；（7）对不同工况条件下细水雾与油池火相互作用的过程进行了模拟实验研究。

Methods One hunderd amd ten human subjects (one hunderd and seventy-four eyes) were included in this research. Retinoscopy was made to measure refractive errors, corneal topographer was measured by corneal topography system, Tonometry was used to measure IOP, Ultrasonagraphy was applied to measure anterior chamber length, len thickness and vitreous length respectively, Laser interference biometer was used to measure axial length, Optical coherence tomography was used to measure retina thickness of fovea area. Based on spherical equivalent subjects were divided into four groups: emmetropia group (SE0.50D), low-myopia group (-0.50D≤SE3.00D), moderate myopia group (-3.00D≤SE6.00D) and high myopia group (SE≥-6.00D).

对近视患者及正视者110例（174只眼），主觉加检影验光行屈光检查，采用Humphrey测量角膜平均曲率，非接触式眼内压测量仪测量眼内压；A与B型超声诊断仪测量前房深度、晶状体厚度、玻璃体腔长度及眼轴长度；角膜测厚仪测量中央角膜厚度(CCT，centercornealthickness)；光学相干生物测量仪测量眼轴长度；光学相干断层扫描仪测量黄斑中心视网膜厚度；按等效球镜度数(SE， sphericalequivalent)不同分正视组(SE0.05D)、轻度近视组(-0.50D≤SE3.00D)、中度近视组(-3.00D≤SE＜-6.00D)、高度近视组SE≥-6.00D）。

It does the more enrapture me,And even so, I still remainA lover in captivity.Greensleeves was all my joy,Greensleeves was my delight,Greensleeves was my heart of gold,And who but my lady greensleeves.3 Greensleeves, now farewell, adieu,God I pray will prosper thee,For I am still thy lover true,Come once again and love me.Greensleeves was all my joy,Greensleeves was my delight,Greensleeves was my heart of gold,And who but my lady greensleeves.

Adrain个人小观点：这首诗简约而又生动忧伤，值得大家注意的是它所描写的故事是一个君王对一个平民女子的爱，在诗里，这位君主的爱超越了阶层，也超越了物质和生死，假设和对比手法的运用将感情抒发到了极致，爱的忧伤不仅仅是忧伤，在诗里也是一种美。

CHAPTER XI OFFERS OF SERVICE FROM MISERY TO WRETCHEDNESS Chinese Marius ascended the stairs of the hovel with slow steps; at the moment when he was about to re-enter his cell, he caught sight of the elder Jondrette girl following him through the corridor. The very sight of this girl was odious to him; it was she who had his five francs, it was too late to demand them back, the cab was no longer there,the fiacre was far away.

十一 穷苦请为痛苦效劳英文马吕斯一步一步慢慢地走上了老屋的楼梯，他正要回到他那冷清清的屋子里去时，忽然看见容德雷特大姑娘从过道里跟在他后面走来。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。