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The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

Bubble nucleating and porosity were improved by adding lignin granule, and cell size of the polyurethane foam was decreased. Not only physical property but also thermal property of the material were enhanced. Compared with soft polyurethane foam, tensile strength of lignin reticulated polyurethane foam was raised by 140% at most which lignin content was 5%, and the heat-degradation temperature at the first stage was 50℃ more than soft polyurethane foam which lignin content was 10%. There had been a great improvement in heat resistance of the material. It was showed that weight of biological film reached to 10000 mg/L and 12100 mg/L when the lignin content of the polyurethane foams was 5% and 8% respectively via 30-day biofilm formation testing.

实验结果表明：1，4-二氧六环对木质素的溶解能力是最好的，可使木质素均匀分散在聚氨酯泡沫塑料中；木质素颗粒的加入可改善发泡过程的成核作用，提高聚氨酯泡沫的开孔率，降低泡沫孔径，并且能提高材料的力学、热学性能，与软质聚氨酯泡沫塑料相比，木质素含量5%的聚氨酯泡沫塑料拉伸强度最大可提高140%，木质素含量10%的聚氨酯泡沫塑料发生第一阶段失重的温度提高了50多度，材料的热稳定性大大提高；经过30 天的挂膜测试，木质素含量5%和8%的聚氨酯泡沫塑料的挂模量可分别达到10000 mg/L和12100 mg/L。

I soon found a way to convince him that I would do him no harm, and taking him up by the Hand laugh'd at him, and pointed to the Kid which I had kill'd, beckoned to him to run and fetch it, which he did; and while he was wondering and looking to see how the Creature was kill'd, I loaded my Gun again, and by and by I saw a great Fowl like a Hawk sit upon a Tree within Shot; so to let Friday understand a little what I would do, I call'd him to me again, pointed at the Fowl which was indeed a Parrot, tho' I thought it had been a Hawk, I say pointing to the Parrot, and to my Gun, and to the Ground under the Parrot, to let him see I would make it fall, I made him understand that I would shoot and kill that Bird; accordingly I fir'd and bad him look, and immediately he saw the Parrot fall, he stood like one frighted again, notwithstanding all I had said to him; and I found he was the more amaz'd, because he did not see me put any Thing into the Gun; but thought that there must be some wonderful Fund of Death and Destruction in that Thing, able to kill Man, Beast, Bird, or any Thing near, or far off; and the Astonishment this created in him was such, as could not wear off for a long Time; and I believe, if I would have let him, he would have worshipp'd me and my Gun: As for the Gun it self, he would not so much as touch it for several Days after; but would speak to it, and talk to it, as if it had answer'd him, when he was by himself; which, as I afterwards learn'd of him, was to desire it not to kill him.

其实，对星期五，我根本用不着采取任何防范措施。任何其他人都不可能有像星期五这样忠诚老实、听话可爱的仆人。他没有脾气，性格开朗，不怀鬼胎，对我又顺从又热心。他对我的感情，就像孩子对父亲的感情，一往情深。我可以说，无论何时何地，他都宁愿牺牲自己的生命来保护我。后来，他的许多表现都证明了这一点，并使我对此毫不怀疑。因此，我深信，对他我根本不用防备。

Under the microenvironments of fruit bagging,Alternaria had always been the major fungi in different bags,sites of fruit and weather conditions during the whole growing season. As the secondary major fungi,Penicillium had always been after July and emerged later in inner bag and cortical pore than other sites.In the bag with which the inner bag was red and waxing,Trichoderma was isolated first in pericarp without cortical pore and inner bag as well as later in cortical pore after August.In \'Kobayashi\' bag which inner bag was red and waxing, Aspergillus and Fusarium were isolated in pericarp without cortical pore and cortical pore after bag removed.Alternaria and Penicillium were isolated in unbagging fruit and \'Qianwei\' bag which inner bag was red and unwaxing and the date Penicillium was isolated in CK was 45d later than bagging treatment.Under high temperature weather condition,there were no differences in the fungi population structure on fruit surface among all treatments.And the fungi population structure on fruit surface in bagging apple was more than that of unbagging ones under overcast and rainy condition.

在苹果套袋微域环境下，不同育果袋、果实不同部位、不同时期、不同气象条件下的真菌主要是链格孢霉，且在套袋后的整个生长季均可分离到；青霉为第二大真菌，在7月份后均可分离到，在非皮孔部位的出现要早于内果袋和皮孔部位；8月份后在内袋红色涂蜡的育果袋上分离出了木霉，其先出现在非皮孔处和内果袋，然后出现在皮孔处；曲霉和镰刀菌仅在内袋红色涂蜡的&小林&袋的非皮孔部位和皮孔部位于摘袋后分离出；在内袋为黑色且未涂蜡的育果袋和未套袋果上仅仅分离出链格孢霉和青霉，且青霉分离出的时期要比套袋果晚45d。

Tantalus was the rich and mighty king of lydia.son ofzeus by a fairy maiden,he was greatly favored by the gods.he was invited to share wine and food with them at their banquets,and he was let into their secrets .but ever the latter end of joy is woe .either because he had stolen wine orgave away the secrets of zeus,or because he had become so proud as to test the gods by serving up the flesh of his son pelopsto them,he incurred the wrath of zeus and was hurled down to the everlasting darkness of tartarus .there he was made tostand up to his chin in a little lake,with clusters of luscious fruits hanging over and around his head.he strove eternally to drink the ever-evading water and to clutch at the delicious fruits which were ever tossed out of his reach by blasts of wind.thus he was doomed to endure endless burning thirst and bitter hunger in the world of the shades.the whole occurrence has given rise to the expression"tantalizing",meaning somethng inplain view but unattainable .

坦塔罗斯是罗底亚富有而又强大的国王。他是宙斯和一位仙女的儿子，深受众神喜爱。众神请他参加宴会，与他们共享美酒与美食，并且还让他了解他们的秘密。然而乐极生悲。或许是他偷了美酒、或者泄露了宙斯的秘密、或许是他太骄傲以至于献上自己儿子珀罗普斯的肉来检验神的智慧，他招致了宙斯的愤怒，被打入了永远黑暗的地狱中。在那里他被迫站定于一小湖中，水没至下颌，无数甜美的水果悬于头顶和四周。他从不间歇地努力喝水，但水总是从嘴边流走；他试图抓住那些美味的水果，但总是被阵阵来风吹离，使他无法得到。因而他注定要在阴间遭受炙渴和难以忍受的饥饿。&tantalizing&一词即由此而来，意思是在眼前却无法得到的某个东西。

Tantalus tantalus was the rich and mighty king of lydia.son ofzeus by a fairy maiden ,he was greatly favored by the gods.he was invited to share wine and food with them at their banquets,and he was let into their secrets .but ever the latter end of joy is woe .either because he had stolen wine orgave away the secrets of zeus ,or because he had become so proud as to test the gods by serving up the flesh of his son pelopsto them,he incurred the wrath of zeus and was hurled down to the everlasting darkness of tartarus .there he was made tostand up to his chin in a little lake,with clusters of luscious fruits hanging over and around his head.he strove eternally to drink the ever-evading water and to clutch at the delicious fruits which were ever tossed out of his reach by blasts of wind.thus he was doomed to endure endless burning thirst and bitter hunger in the world of the shades.the whole occurrence has given rise to the expression"tantalizing ",meaning somethng inplain view but unattainable.

坦塔罗斯坦塔罗斯是罗底亚富有而又强大的国王。他是宙斯和一位仙女的儿子，深受众神喜爱。众神请他参加宴会，与他们共享美酒与美食，并且还让他了解他们的秘密。然而乐极生悲。或许是他偷了美酒、或者泄露了宙斯的秘密、或许是他太骄傲以至于献上自己儿子珀罗普斯的肉来检验神的智慧，他招致了宙斯的愤怒，被打入了永远黑暗的地狱中。在那里他被迫站定于一小湖中，水没至下颌，无数甜美的水果悬于头顶和四周。他从不间歇地努力喝水，但水总是从嘴边流走；他试图抓住那些美味的水果，但总是被阵阵来风吹离，使他无法得到。因而他注定要在阴间遭受炙渴和难以忍受的饥饿。&tantalizing &一词即由此而来，意思是在眼前却无法得到的某个东西。

The average rate of volume shrinkage of oven-dried in Larix gmelini was 6.34%, the average rate of radial shrinkage of oven-dried in Larix gmelini was 1.98%, the average rate of tangential shrinkage of oven-dried in Larix gmelini was 4.23%. Variance of volume shrinkage of oven-dried in Larix gmelini was more significant among trees, and its value ranged from 4.48% to 7.59%. Variance of radical shrinkage of oven-dried in Larix gmelini was not obviously among trees, and its value ranged from 1.27% to 2.69%. Variance of tangential shrinkage of oven-dried in Larix gmelini was more obviously among trees, and its ranged from 2.73% to 5.00%. Difference of value of / among trees was obvious, and its value ranged from 2.15% to 3.58%.

兴安落叶松木材全干体积干缩率的平均值为6.34%，全干径向干缩率的平均值为1.98%，全干弦向干缩率的平均值为4.23%；全干体积干缩率的株间差异较显著，变异范围为4.48%-7.59%，全干径向干缩率株间差异不显著，变异范围为1.27%-2.69%，全干弦向干缩率株间差异较显著，变异范围为2.73%-5.00%；全干弦向干缩率与全干径向干缩率的比值株间差异较显著，变异范围为

When aborted rats in group E were treated with the dose of 50 IU/g IFN-γ, expression of TNF-αin this group was compared with the other four groups, the results were as follows: Compared with group A, the nucleus pre-opticus medialis, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami and pars intermedia had a highly expression of TNF-α, in the other nuclei of hypothalamus, neurohypophsis and adenohypophysis, the change was not significantly, the expression of TNF-αin follicle and corpus luteum were decreased distinctly, the variation in uterus was slight and no difference was exsited; Compared with group B, the expression of TNF-αin nucleus nucleus lateralis hypothalami, nucleus ventromedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis and uterus presented a remarkable enhancement, conversely, the TNF-αexpression in the nucleus pre-opticus medialis, nucleus pre-opticus suprachiasmaticus, nucleus supra-opticus, nucleus pre-opticus mango celluaris, nucleus periventricularis hypothalami, nucleus paraventricularis hypothalami, neurohypophsis, follicle and corpus luteum had been significantly decreased; Compared with group C, the nucleus pre-opticus medialis, nucleus ventromedialis hypothalami, nucleus dorsomedialis hypothalami, nucleus arcuatus hypothalami, adenohypophysis, pars intermedia and uterus had upregulated the expression of TNF-α, however, it was decreased in the nucleus periventricularis hypothalami and corpus luteum; When compared with group D, the expression of TNF-αin nucleus lateralis hypothalami, nucleus dorsomedialis hypothalami, nucleus ventromedialis hypothalami, pituitary, follicle and ovary stroma were obviously enhanced, while in the nucleus supra-opticus and nucleus arcuatus hypothalami, the situation was definitely opposite, in the other parts, the change was not significant.

外源腹腔注射50 IU/g IFN-γ后，同A组相比：E组TNF-α免疫阳性产物在下丘脑视前内侧核、视前大细胞核、室周核、腹内侧核、背内侧核、弓状核、垂体中间部显著增高，在下丘脑其它核团、神经垂体、垂体前叶较A组差异不显著，在卵泡、黄体中阳性产物表达均较A组显著降低，在子宫中表达较A组变化不明显；同B组相比：E组TNF-α免疫阳性产物在下丘脑外侧核、腹内侧核、弓状核、垂体前叶、子宫均显著增高，在视前内侧核、视交叉上核、视上核、视前大细胞核、室周核、室旁核、神经垂体、卵泡、黄体中阳性物质表达均显著降低；同C组相比：E组下丘脑视前内侧核、腹内侧核、背内侧核、弓状核、垂体前叶与中间部、子宫均显著增高，在室周核、黄体中均显著降低；同D组相比：E组下丘脑外侧核、背内侧核、腹内侧核、垂体各部、卵泡、卵巢基质均显著增高，在视上核、弓状核显著降低，在其它部位表达变化差异不显著。

In respect that various requirements of load and temperature on different parts of the baker, firebrick lining was chose reasonably, and the dosage of light heat preservation brick is in enlargement. As a result of that, the weight of the furnace was decreased, and increased its performance of heat preservation and cumulation was increased consumedly. With the use of ""W"" type fire tunnel and straight-through dilatation joint, and two enlargement of the perpends on the wall appropriately, all the problems of heat preservation in the anode baker, uniform temperature in the tunnel, and its life span were solved effectively. In the research of anode blocks in and out of the furnace, and of tidying it up, the multi-function crane clamps was alented and the assembling unit was reconstructed, which made it easy to automatic ally assemble the carbon blocks of various specifications with only one set of assembling unit.In the aspects of automation, a new type pulse combustor and solid thermocouple, as a result of that, not only the control accuracy of the fire tunnel's temperature was increased, but also the system function was improned to be more dependable, safe and practicable.

在焙烧炉炉体研究方面，针对焙烧炉不同部位的负荷及温度要求的不同，合理选用耐火材料，加大轻质保温砖的用量，既减轻炉体的重量，又大大提高炉体的保温蓄热性能；火道墙采用&W&型火道结构，直通性膨胀缝，并适当加大火道墙竖缝，有效地解决了阳极焙烧炉的保温、火道温度均一及火道墙的使用寿命等问题；在阳极炭块装出炉及清理等研究方面，通过对编解机组及多功能天车夹具进行改造，实现一套机组对多规格炭块的自动编组、解组及装出炉问题；焙烧炉自动化控制研发中，采用先进的分散式控制系统，研制开发新型脉冲燃烧器及实体型热电偶，不仅提高了火道温度的控制精度，而且提高了系统的可靠性、安全性、实用性。

From May 4th Movement of 1919 to the 1920s, sex love narrations in Chinese novels were rather an enlightenment and liberation in that particular era with four characteristics. Firstly, sexual desire was endowed with rationality with regard to liberation of human nature and mutual love. Secondly, with an epochal concern for a stronger and superior race, greater attention was paid to sexual parties concerned, mainly physically. Thirdly, new ethics on sex were promoted in that personal freedom on sex love was emphasized and sex love became an important way of self-fulfillment. Therefore, independence of sex love was advocated and the scope of sex love was also widened. Fourthly, the sex consciousness of this period had the mixed features of the past and the contemporary. Sex love narrations in the 1930s and 1940s showed a further exploration on the theme of human nature. First of all, sex was restored to a kind of desire. ShenCongwen probed the importance of harmonious sex love to a sound personality and Shi Zhecunrevealed that sexual inhibition could lead to the distortion, even frenzy of human nature. Bothexplored the influence and functions of sexual desire as human nature upon human personality,giving prominence to the irresistibleness of sexual desire and the significance of sexual desire asa motive. Then, human bodies driven by sexual desire were described and given greater attentionto. Next, ethics on sex were deconstructed either by Shen Congwen who opposed man-madeethics with law of nature and the School of New Sensation who replaced traditional ethics on sexwith exchange rules in consumption, or by Shi Zhecun who spared no efforts in representing thecomplete failure of social rules, including ethics on sex, due to the impact from sexual desire.The direct effect was a paleness of the ethical binding force on sex. Last, sex consciousness inthe works of female writers emerged. In the early works of Ding Ling, the sexual desire of thefemale is no longer a ramification of emotion but an independent one. In addition, in the sex lovenarrations of other female writers, such as Mei Niang, strong self-consciousness on the socialstatus of the female is also reflected. Sex love narrations in the late 1970s and early 1980s wereactually a re-enlightenment in the spirit of humanism, whose major features include humanisticlegitimation of sex love within the boundary of ethics, a blend of sex love narrations withnational concepts and national myths as well as the aesthetic, poetic descriptions of humanbodies etc. In the middle and late 1980s, sex love narrations entered a stage of exploration forpluralism. First, by restoration of sexual desire, writers of this era restored human nature, thevery original state of human being. Different emphasis and tendencies could be seen in differentwriters: looking at sex love dialectically and trying to describe its creative as well as destructivecharacteristics; connecting sex love with violence and trying to reveal the evil side of humannature through it; emphasizing the creativeness of sex love especially in the heritage of"wildness".

从&五四&到20世纪20年代，中国小说的性爱叙事是一种处在时代夹缝中的启蒙和解放，其所表现出来的特点，一是正面赋予性欲望以合理性，这种合理性是在人性解放和爱情前提下获得的；二是在强种育种的时代焦虑影响下小说性爱叙事表现出对物质身体的关注，其焦点主要在体格方面；三是树立起新的性伦理道德观念，表现为强调在性爱问题上的个人选择自由，性爱成为个人自我实现的重要方式，从而赋予人在性爱问题上的独立性，同时还扩大了性爱表现的领域；四，这个时期小说性爱叙事中所表现出的性意识还具有新旧杂糅的特点。30、40年代小说性爱叙事表现出对人性主题的深化与丌拓，首先是把性还原为欲望本身来探索，沈从文从和谐性爱对健全人性的决定作用、施蛰存从性压抑会导致人性扭曲甚至疯狂的角度探讨作为人类本质性欲望的性对人性产生的影响和作用，突出了性欲力量的不可抗拒和作为人类行为的重要动力等方面的内容；其次在叙事中表现出对欲望化了的身体的描绘与关注；第三是解构了性的伦理道德，无论是沈从文以自然法则来反对人为的道德设置还是新感觉派用消费交换原则替代传统性伦理道德，或是施蛰存极力表现包括性伦理规范在内的社会法则在性欲力量冲击下溃不成军，其直接的效果都是使性伦理道德对人的约束力量显得苍白虚弱；第四，出现了由女性作家表现的女性性意识，在丁玲的早期作品中女性的性欲望不再是作为感情的衍生物而是独立的欲望存在，而在梅娘等其他女作家的小说性爱叙事中则表现出对女性社会地位的强烈自觉意识。70年代末80年代初期小说的性爱叙事实际上是在人道主义语境下的再次启蒙，其主要特征一是性爱在人道主义前提下获得合法性，但要求性爱不超出伦理规定的范围；再就是将性爱叙事同国家观念和民族神话相融合；此外还有对身体的描写审美化诗意化等。80年代中后期，小说性爱叙事进入了多元化探索的阶段，作家通过还原性欲望来还原人性、还原人类的本真状态，不同作家笔下表现为不同的侧重和倾向——以辨证的方式看待性爱，写出其具有的创造性力量和毁灭性特征；将性爱与暴力相联系并通过其来表现人性的黑暗；强调性爱的创造性作用，尤其在民族&血性&的传承方面。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。