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The index case was a 5-month-old infant girl who had anisocoria from 4 weeks of age. One hour after 1% apraclonidine hydrochloride was instilled in each eye, there was blanching of the periocular skin, and the parents reported that the infant was excessively drowsy. Two hours after receiving apraclonidine, the infant was taken to the emergency department for lethargy and unresponsiveness, with bradycardia (84 beats/min), shallow respiration (40 breaths/min), and blood pressure 115/85 mm Hg. Oxygen saturation was 80% but improved rapidly to 100% when oxygen was given via a face mask. Eight hours after receiving apraclonidine, the infant had returned to normal.

指标案例是一名5个月大的女婴，她从4周大时就有瞳孔大小不等的状况；在1%的apraclonidine氯化氢徐徐滴点她的双眼后1个小时，眼眶周边的皮肤即变白，而她的家长表示她变得非常嗜睡；在使用apraclonidine 2个小时之后，该名女婴因嗜睡和无应答性，以及心搏过慢（每分钟84下）、呼吸浅弱（每分钟40次）以及血压115/85 mm Hg等症候而被送进急诊室；血氧饱和度原先为80%，在面罩给氧后迅速回复到100%，在使用apraclonidine 8个小时后，该名女婴终於回复健康状态。

Grew on the normal temperature, it was shown that the deposits of calcium antimonate being the indicator for Ca(superscript 2+) localization mainly concentrated within the vacuoles and intercellular spaces and there was also some Ca(superscript 2+) deposits in cell walls. But when Garyota urens L. was treated by the temperature of 2℃ for 48 h, the level of Ca(superscript 2+) increased in cytoplasm and plasma membrane, but decreased in vacuoles and intercellular spaces considerably. At the same time, the ultrastructure of chloroplasts suffered from chilling: the membrane of chloroplasts had been damaged, the layer of thylakoids was exiguous and unclear, the photosynthetic rate decreased evidently. And when Garyota urens L. was treated by the temperature of 2℃ for 120 h, the deposits of Ca(superscript 2+) mainly concentrated within the cytoplasm, nucleus and plasma membrane and there was also some Ca(superscript 2+) deposits in vacuoles, and the ultrastructure of some cells was simultaneously damaged severely: Chloroplasts structure, vacuole membrane and nuclear membrane had been damaged fully, the structure within the cell had become unclear, and the cell only have respiration.

研究结果表明，未经低温处理的董棕幼苗叶肉细胞，焦锑酸钙沉淀颗粒大量出现在液泡和细胞间隙中，细胞壁中也可见少量沉淀，而细胞基质中则看不到焦锑酸钙沉淀；经2℃ 48 h低温处理后，细胞基质和细胞膜上焦锑酸钙沉淀增加，而液泡和细胞间隙中的焦锑酸钙沉淀则显著减少，并且超微结构已初步显示出寒害的特徵，叶绿体外膜部分破损，类囊体片层稀疏且排列不规则，光合速率明显下降等；经2℃ 120 h低温处理后，细胞间隙内的焦锑酸钙沉淀极少，有的也紧贴在细胞外壁上，而细胞基质和细胞膜上则分布有非常多的焦锑酸钙沉淀，在核基质和液泡中也可见到少量的焦锑酸钙沉淀，并且超微结构遭到了显著破坏，叶绿体结构完全被破坏，核膜与液泡膜严重破损，内部结构模糊，细胞只表现为呼吸作用，不进行光合作用。

The factors which affect the average molecular weight of the polymer of AM and DMDAAC were investigated. The optimal reaction conditions are as follows: the concentration of monomer AM was 30%, while DMDAAC was 5%; the reaction temperature was 30℃, the amount of initiators at the low temperature stage was 0.05‰(compared to the weight of the monomers joining the polymerization), while V-50 was 0.025‰, the weight ratio of potassium persulphate to sodium bisulphite is 1:1, the amount of carbamide was 40‰, and complexing agent was 0.05‰, besides, aerate nitrogen gas for 8min, then the high molecular weight polymer (M=1.217×10^7) could be got after 16h reaction.

研究了影响AM与DMDAAC共聚物的平均分子量的因素，得到了优化条件为：单体浓度为30%，DMDAAC与AM摩尔比为5：95，聚合温度为30℃，低温段引发剂用量为0.05‰，K2S2O8与NaHSO3质量比为1：1，偶氮二异丁基脒盐酸盐(V-50)用量为0.025‰，尿素用量为40‰，乙二胺四乙酸二钠(EDTA-2Na)用量为0.05‰，在此优化条件下聚合反应16h后得到分子量为1.217×10^7的阳离子型聚丙烯酰胺。

The AMF spore average density was 24.88 per 20 g soil , the species richness was 1.94 per soil sample, the species diversity index was 2.56, and the species evenness was 0.65. Glomus, but they all were of prominent genus in the investigation area and Acaulospora, Scutellospora were of common genus while Entrophospora was rarely found. In the 13 species AM fungi, G.mosseae was of prominent species, and G.geosporum, G.intraradices, G.etunicatum were of common speices, while the others were of rarely species. There were 87.5% sand fixation plant were infected with arbuscular mycorrhizal fungi, the highest spore density, the strongest infection strength of the whole roots and the best species richness belonged to Chenopodiaceae while Polygonacea was the lowest.

四属AM真菌中，Glomus为优势属， Scutellospora 、Acaulospora为常见属，Entrophospora则为稀有属；十三种AM真菌中，G.mosseae、G.geosporum分别为优势种、最常见种，G.intraradices，G.etunicatum为常见种，其余均为稀有种；五科固沙植物中，87.5%的植物能被菌根侵染，其中藜科植物孢子密度最高，整个根系侵染强度最强，种的丰度最高，蓼科植物孢子密度、整个根系侵染强度、种的丰度则最低；禾本科植物不仅具有最好的丛枝结构，且具有相对高的亲和性，能与12种AM真菌共生。

The growth inhibiting rate of T24 cell lines were detected by MTT methods, apoptosis of cells were detected by flow cytometry, the mechanism of apoptosis was analyzed by detecting the protein expression of Bcl-2, Bax, Caspase-9, Caspase-3 and cytoplastic protein Cytochrome C. 4 We injected live T24 cells into the subcutaneous space of nude mice and successfully built up the animal model of bladder carcinoma. The effect of CS-PAA-EPI polymer magnetic microspheres targeting chemotherapy was investigated by HE staining, TUNEL ,tumor weight and volume inhibition rate. Results: 1 TEM revealed that the CS-PAA polymer magnetic microspheres were regular spherical shape,the average diameter was 80nm in dry condition. By controlling the pH value of the medium,polymers had positive or negative zeta potential. VSM showed the CS-PAA polymer magnetic microspheres had superparamagnetic. The diameter of CS-PAA-EPI polymer magnetic microspheres were 200nm in solution by DLS examining,the embedding ratio was 20%,the EPI loading rate was 15%, which was higer than reported in other articles. 2 Raw eye observation found that the rat"s bladder of treatment group was brown color,which meaned the aggregation of iron particles, compared with the control group, iron stain found iron particles were assembled in rat"s bladder of the treatment group, the amount of iron particles in liver and spleen were less obviously.

研究结果：1合成的CS-PAA磁性聚合物微球呈球形，大小均一，TEM测定其干态下粒径为80nm左右，磁化曲线证实具有超顺磁性，具有一定的PH敏感性，固载表柔比星后，水溶液性状稳定，无沉淀物，DLS测定直径约200nm左右，测定载药率为15％，较文献报道高，包封率为20％。2肉眼观察试验组大鼠膀胱表面呈褐色，可见大量的Fe粒子聚集，普鲁士兰染色法显示，试验组大鼠膀胱壁内有大量的Fe粒子，分布至膀胱壁全层，与对照组大鼠相比，试验组大鼠的肝、脾内的Fe粒子聚集量明显降低；HPLC测定结果与Fe染色相同；高剂量磁性CS-PAA-EPI生理盐水组及单纯EPI生理盐水组均在给药后14天出现血肌酐和尿素氮的升高，其他组大鼠血生化指标没有明显变化。3MTT法发现，高、中、低剂量磁性CS-PAA-EPI生理盐水组在外加磁场的协同作用下杀伤T24细胞效应明显高于单纯的EPI生理盐水组，FCM发现试验药物组可引起明显的肿瘤细胞凋亡，试验药物治疗组细胞胞浆内出现了由线粒体释放出的细胞色素C，试验组细胞Bcl-2蛋白减少，Bax蛋白变化不明显，Caspase-3、Caspase-9蛋白受到了激活活化。4高、中、低剂量磁性CS-PAA-EPI生理盐水组的瘤重抑制率和瘤体积抑制率均明显高于单纯的EPI生理盐水组(P＜0.01)，其中高剂量组的抑制率最高。

The relevant experiment was also conducted. Results In treatment group, the general symptom eliminating time was 11.2±4.7 dags; in intensive phase,the adverse reaction occured in 714 cases in all;sputurn negative conversion rate was 93.33%;drug resistance rate was 5%;X-ray obviously absorbing rate was 68.3%;cavity closing rate was 77.8%;alter treatment completed,cure rate was 96.67%.

结果 治疗组结核病人的一般症状消失时间为11.2±4.7天；在强化期内，化疗药物毒副反应发生率为14人次；痰菌阴转率为93.33％；耐药菌株发生率为5％；病变X线明显吸收率为68.30％；空洞闭合率为77.80％；全部疗程结束后，其综合疗效达96.7％。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

On admission, he was alert and fully oriented. His temperature was 38°C, pulse was regular (90/min), blood pressure was 150/80mmHg. His speech was severely dysarthric but he could name, repeat, read, and follow instructions. His cranial nerves and fundoscopic examination were normal. His gait was wide based and unsteady. He had four sided mild weakness, which was prominent on the right.

入院时体格检查示：T 38°C，脉搏规律90次/分，血压150/80mmHg；双下肢前方见暗红色疼痛性结节性红斑；眼科检查未见眼葡萄膜炎；患者主诉阴囊发热、疼痛，检查时见双侧附睾肿胀、质硬、明显触痛，呈附睾炎表现。

The results showed:①the communities that stipa baicalensis is edificator had the highest species diversity, and the idea that stipa baicalensis communities were the local zone vegetation was supported;②the majority of studied communities were unsaturated in the species diversity, and the species diversity had a tendency to increate with the improvement of soil environment;③the relation of species diversity to community productivity was a one-humped function, that is, the species diversity of mediation productivity was highest;④the species diversity under intermediate disturbance was highest, and the intermediate disturbance hypothesis was supported;⑤the content of potassium in soil had probably an important function, or was an important following factor in the types of grassland community;⑥a new hypothesis on the forming mechanism of species diversity of community, Small Scale Competition and Random-Patch Dynamic Hypothesis, was put forward boldly.

通过上述研究分析得出如下结果：①以贝加尔针茅为建群种的群落物种多样性最高，从物种多样性角度证明该群落类型最适应本区气候和环境条件，应是本区的地带性群落；②绝大多数群落的物种多样性处于未饱和状态，随土壤环境的改善呈明显的增加趋势；③物种多样性与群落生产力呈单峰函数关系，即在中等生产力水平时物种多样性最高；④放牧中等干扰水平物种多样性最高，支持Connell的中度干扰学说；⑤偶然发现土壤钾含量可能在草地植被类型分化中起到重要作用，至少是一个重要的伴随因子；⑥大胆提出了群落物种多样性构成机制的新学说即小尺度竞争随机斑块动态学说。

Ying was abandoned by his parents when he was only weight 3 pound ; Zou was abandoned by his parents more than 50 days after he was born, he has serious heart problems, pulmonary hypertension and facing life-threatening; Snow is a child with cerebral palsy and has been abandoned; Zhao has serious equinus varus ; Lu leads to congenital absence of anus, this has not yet one month old baby was found in the trash can , and when he was sent to the orphanage he was dying; Run had been abandoned in a county hospital, only because she is a cleft lip and palate baby...

党英被父母遗弃时体重只有三斤多点，蔫蔫的；小邹成出生后被父母遗弃在山东省邹城县医院的过道上长达50多天，严重的心脏病致使他心内膜垫完全缺损、肺动脉高压，面临生命危险；瑞雪是脑瘫患儿，出生时由于硬肿症送到医院抢救，半岁后孩子出现脑瘫迹象，所以被遗弃；赵恒有严重的马蹄足内翻；路通先天无肛，这个还没有满月的娃是在路边的垃圾桶里被人发现的，送到福利院时已经奄奄一息；润润曾经被遗弃在河南一个县城医院里，只因为这是一个唇腭裂的宝宝。。。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。