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Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.
方法1、药敏实验和三维实验收集2003~2007年临床分离的220株Pa,对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况,同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration,MIC),筛选出对亚胺培南耐药的铜绿假单胞菌,并分析其对其它抗生素的药物敏感率;采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株,采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因,采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因,用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况;同时对产AmpC酶的Pa(25株,含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。
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The optimum reaction conditions were that the molar ratio of maleic anhydride and p-nitroaniline was 1.2:1, the volume ratio of toluene and DMF was 10:1, the amination temperature was 20~30℃ the cyclization temperature was 145~150℃ and the cyclization time was 6 h over; the amount of p-toluene sulfonic acid was 12% of total reactant mass, the amount of copper sulfate was 0.1~0.5% of total reactant mass, the amount of hydroquinol was 0.1% of total reactant mass.
得到的最佳工艺条件是:n:n为1.2:1,V:V为10:1;胺化反应温度为20~30℃,环化反应温度为140~150℃,环化反应时间为6h以上;对甲基苯磺酸用量为反应物总质量的1%~2%;硫酸铜用量为反应物总质量的0.1%~0.5%;对苯二酚用量为反应物总质量的0.1%。
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The results showed that the optimum operating parameters for steaming treatment were as follows: temperature was 95℃, time was 60 s, for tunnel-type drying production line, the loadage was 5.0 kg/m2, hot air temperature was 85℃, wind speed was 1.5 m/s, and for industrial microwave drying production lines, material thickness was 15 mm, drying power was 16 kW, drying time was 90 s.
结果表明,在蒸汽温度95℃,汽蒸时间60 s;隧道式干燥生产线装载量5.0 kg/m2,热风温度85℃,风速1.5 m/s;微波干燥生产线铺料厚度15 mm、干燥功率16 kW、干制时间90 s的条件下,所生产的干制黄花菜经农业部食品质量监督检验测试中心检测其感官、理化和微生物指标均达到了《无公害食品—干制金针菜》NY5186-2002所规定的一级品标准。
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Results showed that the kinetics of photocatalytic degradation of ethylene by TiO2/ACF desoposited Pt in the simulated cold storage environment for horticultural products could be described using the pseudo first-order equation. The prepared TiO2/ACF-Pt, in the process of which Pt was initially deposited on the surface of ACF and then TiO2 was coated, would promote the activity of ethylene decomposing. When ion sputtering time was 60 seconds (Pt/Ti atomic concentration ratio was 0.112), the apparent rate constant of the reaction of TiO2/ACF-Pt was 1.16 times as much as that of TiO2/ACF. The micrographic structure of ACF was not damaged and an excessive porous structure, which was contributive to absorbing the diluted ethylene, was obtained by the way of ion sputtering Pt.
结果表明:在模拟园艺产品冷藏环境中,TiO2 /ACF-Pt光催化降解乙烯的动力学可用表观一级速率方程来描述;ACF表面先溅射沉积Pt,再进行TiO2附着方案,能提高降解乙烯的能力,当溅射时间为60 s(Pt/Ti原子浓度比为0.112)时,表观一级速率常数是未溅射Pt的1.16倍;TiO2/ACF-Pt的制备方法,对ACF形貌结构没有破坏,并能得到有利于对微量乙烯的吸附的多孔结构。
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But the effluent ammonium in the anoxic reactor, where enough NO2 were present, was equal to the blank system, and no ammonium was converted to such nitrogen compounds as NO2- and N2 by Nitrosomonas eutropha using NO2 as electron acceptor, which maybe caused by lack of the function bacteria. There were two ANAMMOX reaction pathways in the one-stage autotrophic nitrogen removal system. One way was that after part of NH4+ was oxidized to NH2OH under aerobic conditions, NH2OH and NO2- were converted to N2O under anaerobic conditions, at last N2O was further converted to N2 which realized the nitrogen removal; Another way was that at first NO2- was reduced to NH2OH, NH2OH reacted with NH4+ to form N2H4, which was further converted to N2 subsequently, realizing the nitrogen removal.
结果表明:单级自养脱氮系统内6.72%的氨氮是通过吹脱等物化作用去除的,不超过6.02%的氨氮是通过传统硝化反硝化途径去除的,87.26%左右的氨氮是由自养脱氮途径去除的,自养脱氮反应起主要脱氮作用;在足够NO2存在且缺氧的条件下,单级自养脱氮系统内的出水氨氮浓度与空白反应器相当,NH4+并没有被亚硝化单胞菌以NO2为电子受体氧化为NO2-和N2等化合物而得以去除,可能是因为系统内不存在该代谢功能的亚硝化功能菌;单级自养脱氮系统内存在两条ANAMMOX反应途径:其中一条途径即NH4+在好氧条件下被氧化为NH2OH后,生成的NH2OH与系统内的NO2-在缺氧条件下被转化为N2O,N2O则进一步被转化为N2而实现氮的去除;另外一条途径即NO2-首先被还原为NH2OH,生成的NH2OH则与系统内的NH4+反应生成N2H4,N2H4继续被转化为N2而实现氮的去除。
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Results:92 patients (113 ears) were examined by PTHT, AI , 40 Hz AERP,ABR and DPOAE. It was found that organic deafness was confirmed on 98 ears (86.73%), of which exaggerated hearing loss was 38 ears; nonorganic deafness was confirmed on 13 ears (11.50%); stimulated deafness was 2 ears(1.77%). 98 organic deafness ears were diagnosed, of which conductive hearing loss was 25ears; sensorineural hearing loss was 29 ears, and mixed hearing loss was 44 ears.
结果: 92例患者113耳行纯音听阈测试、声导抗、40 Hz AERP、ABR与DPOAE检测,发现器质性聋98耳,占86.73%,其中夸大性聋者38耳,夸大程度<20dBnHL6耳,20~40dBnHL27耳,>40dBnHL5耳;非器质性聋13耳,占11.50%,诈聋2耳,占1.77%;98耳器质性听力损失者中,25耳为传导性耳聋,29耳为感音神经性耳聋,44耳为混合性耳聋。
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Results The resection rate for low and undifferentiated adenocarcinoma and mucinous carcinoma on gastroscopic biopsy was low (64.1%), and the rate for diffuse infiltrative gastric carcinoma (6.7%) was significantly lower than that of mass tumor type (66.7%) and ulcerative type (61.8%). The total accuracy rate of CT staging was 91.21%. Sensitivity rate of CT diagnosis of perigastric invasion and/ or metastasis was 72.22%. For lymph node staging the accuracy rate of CT was 74.2%, sensitivity rate was 74.1% and specificity rate was 74.3%. CT diagnosis of lymph node groups was blur.
结果 胃镜活检低分化腺癌和黏液腺癌手术切除率(64.1%)较低,弥漫浸润型胃癌的切除率(6.7%)明显低于肿块型(66.7%)和溃疡型(61.8%);CT分期总准确率为91.21%,CT诊断对胃周脏器受侵和转移的敏感性为72.22%,CT对淋巴结分期的准确率为74.2%,敏感性为74.1%,特异性为74.3%,CT诊断对淋巴结分组比较模糊。
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Personality traits were evaluated by Eysency Personality Questionnaire that was compiled by Eysency and revised by Gong. Standard scores of Psychoticism, Extraversion and Neuroticism were calculated, and taking 50 points as a boundary. Each dimension was assigned into 2 grades non-psychotic type (P0) psychotic type (P≥50), introversion type (E0), extraversion type (E0), non-nervous type (N0) and nervous type (N≥50). 5 mL ulnar vein blood was obtained without adding decoagulant, and then serum was removed. Genome DNA was extracted from blood clot by conventional chloroform for PCR analysis. PCR product was sequenced by pyrophosphoric acid. SNP was determined by PSQ96 MA sequenator. Allele frequency was analyzed by mass spectrometry.
人格评定采用Eysency编制,龚耀先修订的艾森克人格问卷、计算精神质,内外问,神经质3个个性维度的标准分,并以50分为界,将各维度分为两个等级:非精神病倾向型(精神质0)、精神病倾向型(精神质≥50);内倾型(内外向0),外倾型(内外向≥多50);非常经质倾向型(神经质0),神经质倾向型(神经质≥50)取肘静脉血5 mL,不加抗凝剂,弃血清,血凝块常规酚氯仿提取基因组DNA,进行PCR分析PCR产物应用焦磷酸测序PSQ96 MA测序仪进行SNP检测,用质谱法进行及等位方基因频率分析。
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Fe and Al in the system were removed by precipitation at pH≈5. Co2 was obtained by precipitation at pH=9.5~10, it was then purified by reprecipitation. Co3O4 was obtained by calcining Co2 at 500℃ for 2h, the purity of which was 99.3%. The recovery rate of Co was 97%. Li2CO3 was obtained by adding Na2CO3 to the mother liquor, the purity of which was 97.8%. The one-time recovery for Li was 73%. The mother liquor can be reused after being cooled to below 10℃ and Na2SO410H2O is recovered.
体系中的Fe、Al从杂质在pH≈5时沉淀除去;Co2在pH为9.5~10沉淀得到,经酸溶解后二次沉淀提纯,500℃煅烧2.0h得Co3O4,其纯度达到99.3%,钴的回收率达97%,母液中Li离子加Na2CO3处理,沉淀得Li2CO3,其纯度达97.8%,锂的一次回收率达73%,母液经冷却至10℃以下除去Na2SO410H2O后可循环使用。
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The optimum condition of preparing process were obtained: the amount - of - substance ratio of sodium hydroxide and eerie sulfate tetrahydrate was 4.8:1 ,the quantity of PEG was 8 mL in reaction and 7 mL in washing, absolute alcohol was 10 mL in washing, irradiation time was 6 min, constant microwave irradiation pressure was 0.14 MPa, microwave power was 232 W, the ultrasonic wave dispersing time was 10 min and the microwave drying time was 10 min.
通过考察制备过程中的各个影响因素,得到了制备纳米二氧化铈的最佳工艺条件:氢氧化钠与四水硫酸高铈物质的量比为4.8:1;聚乙二醇的反应用量为8mL,洗涤用量为7mL;无水乙醇的洗涤用量为10mL;微波恒压时间为6min;微波压力为0.14MPa;微波功率为232W;超声波分散时间为10min;微波干燥时间为10min。
- 相关中文对照歌词
- When Everything Was What It Was
- Sun Was High (So Was I)
- Jesus Was Our Saviour And Cotton Was Our King
- Before There Was Atlanta, There Was Douglasville
- Phil Cosby (Before There Was Atlanta, There Was Douglasville)
- I Didn't Say I Was Powerful, I Said I Was A Wizard
- All I Was Looking For Was You
- Hò Bha Mi, Hé Bha Mi (Hò I Was, Hè I Was)
- The Moon Was Yellow (And The Night Was Young)
- Stella Was A Diver And She Was Always Down
- 推荐网络例句
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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.
在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。
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In fact,I've got him on the satellite mobile right now.
实际上 我们已接通卫星可视电话了
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The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.
世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。