查询词典 wapiti

In order to cluone, seauence and express the outer membrane protein 25 (Omp25) gene sequence of wapiti's Brucella abortus strain ML.

目的 克隆马鹿布氏杆菌ML分离株25kDa外膜蛋白(Omp25)基因，并在大肠杆菌中表达。

The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

It can succeed in making wapiti sperm capacitation in vitro by use of the method sperm-up.

采用上浮法可使马鹿的精子体外获能。

Objective To study the ultrastructural changes of wapiti oocytes and provide theoretical basis for wapiti breeding arid reproduction.

目的 通过对马鹿卵母细胞的超微结构进行研究，旨在为马鹿繁殖育种的研究提供基础理论依据。

The objective of this study was Gansu wapiti in the Sunan deer farm, Studied the antler growth laws and characteristics of Gansu wapiti.

选择肃南鹿场的甘肃马鹿为研究对象，系统研究了甘肃马鹿生茸特性及其规律。

Wapiti sperm capacitation in vitro and its ultrastructural change were studied in order to choose a set of perfect and feasible protocol of wapiti sperm capacitation in vitro, and to provide a basis data on the research of wapiti capatitation in vitro and the mechanism of fertilization.

本研究以马鹿为试验对象，开展了对鹿精子体外获能以及获能前后精子超微结构变化的研究，以期筛选出在实践中具有可操作性且效果良好的马鹿精子获能培养体系，从而为鹿体外受精和受精机理的研究奠定基础。

With 50 thousand head of wapiti, 25 large-scale feed lots of the company produce treasure pilose antler, which is the largest feedlot in China. The total amount of wapiti on hand accounts for 55% of the total amount of that in China,with a progressive increase by 17% annually .

公司拥有 25个大型鹿场，驯养中国茸鹿之花--塔河马鹿70000头，是全国最大的马鹿饲养基地，马鹿存栏头数占全国55%以上，并以每年17%的速度递增。

Depended on resources superiority of wapiti with unique features, serial products of wapiti and dozens of Chinese patent medicines were developed, which many a time won the national prizes.

依托自身独特的马鹿资源优势，多年潜心研发了二十多种马鹿系列产品、数十种中成药品，产品多次荣获国家级奖项。

To make the wapiti sperm capacitated in vitro, we selected and used the method of sperm-up. By making the rate of acrosome reaction as a main index, and the sperm energy, the survial time and the fertilizated rate of wapiti sperm and hamster mature oocytes as auxiliary indexes, it were carried out to compare the effects of wapiti sperm capacitation in vitro under different temperature, different density of pyruvate sodium, caffeine and heparin and different culture medium.

对马鹿精子，使用上浮法诱导其获能，通过对顶体染色测定精子的顶体反应率并作为主要指标，同时以精子活力、精子存活时间和异种受精率为辅助指标，对精子的获能效果进行评价，以比较不同培养温度、丙酮酸钠浓度、咖啡因浓度、肝素浓度以及培养基对马鹿精子体外获能效果的影响。

It shows that the Tianshan wapiti has a close relation with the northeast wapiti, but the Talimu wapiti has a far relationship among the Tianshan wapiti, the Aertai wapiti, the gansu wapiti, the northeast wapiti and the Zuojia wapiti. Furthermore, the Talimu wapiti has the farthest relationship with Tianshan wapiti.

结果表明：天山马鹿和东北马鹿的亲缘关系较近，塔里木马鹿同天山马鹿、阿尔泰马鹿、甘肃马鹿、东北马鹿、左家马鹿的亲缘关系较远，塔里木马鹿和天山马鹿的亲缘关系最远。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失