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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

Along 郑 cantonal and each a street so always of hold the hand to walk, walk from setting sun to moon, walk to the stars, walk to the hush that there night change faintness!

沿着郑州的每一条街道就那样一直的手牵手走着，从夕阳走到月亮，走到星星，走到那夜变的静寂和模糊！

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除了不断追求具有时尚流行感但又多样化的独特创新款式，让女性能够充分展现自我性格美学以及淑女的成熟稳重，更不忘打造符合高跟鞋力学原理，能让行走健康舒适的版型结构，细心呵护每双脚，并以最严苛的各种国际标准测试把关品质，确保每双鞋同时具有内在与外在的精美质感，让每个消费者都能穿的舒适、健康、自信，散发与众不同的新现代女性魅力风采。

Midnight gettin' uptight where are you You said you'd meet me now it's quarter to two I know i'm hangin' but i'm still wantin' you Hey jack it's a fact they're talkin' in town I turn my back and you're messin' around I'm not really jealous don't like lookin' like a clown I think of you ev'ry night and day You took my heart then you took my pride away I hate myself for loving you Can't break free from the the things that you do I wanna walk but i run back to you that's why I hate myself for loving you Daylight spent the night without you But i've been dreamin''bout the lovin' you do I won't be as angry 'bout the hell you put me through Hey man bet you can treat me right You just don't know what you was missin' last night I wanna see your face and say forget it just from spite I hate myself for loving you Can't break free from the the things that you do I wanna walk but i run back to you, that's why I hate myself for loving you I hate myself for loving you Can't break free from the things that you do I wanna walk but i run back to you that's why I hate myself for loving you I think of you ev'ry night and day You took my heart then you took my pride away I hate myself for loving you Can't break free from the the things that you do I wanna walk but i run back to you that's why I hate myself for loving you I hate myself for loving you I hate myself for loving you

午夜时分，气氛正好你在哪里？你说会来找我但现在已经一点四十五分我明白自己犹豫不决，但我依然需要你嘿，杰克，人们真的议论纷纷我置若罔闻，你却越闹越大我不是真的吃醋只是不想被人当小丑看待我日日夜夜都想着你你带走了我的心，然后又夺走了我的尊严我恨自己爱上了你无法从你的所作所为中泰然自若我曾经离开，却又回到你身边那就是我恨自己爱上了你的原因白天了，我没有你而过了一晚但我一直梦想着你的爱你对我那麽糟，但我已经没那麽生气了嗨，老兄，我打赌你会对我好你只是不清楚昨晚错过了什麽我想看看你，告诉你忘掉这些不愉快我日日夜夜都想着你你带走了我的心，然后又夺走了我的尊严 [media=300，50，false，false]http：//file2.engok.com/download/ZipMp3100415/20047-1.mp3 [url=http：//file2.engok.com/download/ZipMp3100415/20047-1.mp3]http：//file2.engok.com/download/ZipMp3100415/20047-1.mp3

Tab 19:51:49 Joan Jett - I Hate Myself For Loving You Midnight gettin' uptight Where are you You said you'd meet me now it's quarter to two I know I'm hangin' but I'm still wantin' you Hey Jack It's a fact they're talkin' in town I turn my back and you're messin' around I'm not really jealous don't like lookin' like a clown I think of you ev'ry night and day You took my heart then you took my pride away I hate myself for loving you Can't break free from the the things that you do I wanna walk but I run back to you that's why I hate myself for loving you Daylight spent the night without you But I've been dreamin''bout the lovin' you do I won't be as angry 'bout the hell you put me through Hey man bet you can treat me right You just don't know what you was missin' last night I wanna see your face and say forget it just from spite I think of you ev'ry night and day You took my heart then you took my pride away I hate myself for loving you Can't break free from the the things that you do I wanna walk but I run back to you, that's why I hate myself for loving you I think of you ev'ry night and day You took my heart then you took my pride away I hate myself for loving you Can't break free from the the things that you do I wanna walk but I run back to you that's why I hate myself for loving you I hate myself for loving you Can't break free from the the things that you do I wanna walk but I run back to you that's why I hate myself for loving you I hate myself for loving you I hate myself for loving you Tab 19:52:00我听一首复制一首歌词给你们哈 Tab 19:52:08反正我是看不懂滴资深男人 19:52:31你看不懂就别发了哈丝妮雅 19:52:35呵呵,Tab进步哦资深男人 19:52:53东方,你要给我介绍女人么 Tab 19:52:56 Tab

19：53：02不发就不发吧丝妮雅 19：53：19我想你用不着我介绍吧资深男人 19：53：34我很是用的着啊丝妮雅 19：53：48哪方面用得着？资深男人 19：54：03哪个方面都用的着资深男人 19：54：09你是海南的？丝妮雅 19：54：30开玩乐，资深，已经够格哦丝妮雅 19：54：42对啊，欢迎来海南玩资深男人 19：54：46哈哈资深男人 19：55：02也欢迎你有时间到西安玩啊 Tab 19：55：22 I'm alive 丝妮雅 19：55：24西安古城，早就想往啦资深男人 19：55：36那怎么还不来啊丝妮雅 19：55：45 TAB，又说什么啊？丝妮雅 19：55：56没人民币啊资深男人 19：55：56 TAB想说话了资深男人 19：56：31美元也是可以的，不是非得用RMB啊 Tab 19：56：42不说，听话丝妮雅 19：56：55呵呵，还没见过美元呢资深男人 19：57：16难道你一直用英镑？丝妮雅 19：57：33我一无所有，好可怜资深男人 19：57：41或者是欧元 Tab 19：57：41呵呵资深男人 19：57：44呵呵 Tab 19：57：49老板不用谦虚丝妮雅 19：58：05呵呵，舒服，有人称我老板啦资深男人 19：58：24这个群里还是南方人多哈 Tab 19：58：32是老板嘛丝妮雅 19：58：32是吗？ Tab 19：58：36大小事自己做主 Tab 19：58：42俺四川人在广州 Tab 19：58：47不是南方银资深男人 19：58：56你不要说俺行不？》丝妮雅 19：59：01四川不算南方吗？资深男人 19：59：08我是山东人在西安 Tab 19：59：11不算 Tab 19：59：16西南方资深男人 19：59：23我还没说俺这个字呢 Tab 19：59：26南方从湖南才开始算丝妮雅 19：59：55不对，起码也是西南了丝妮雅 20：00：17山东人怎么跑到西安啦？资深男人 20：00：25不可以么？

We are now talking about going after the one-year-old more appropriate, the book is also too easy to walk short-sighted eyes, my daughter did not practice before the one-year-old to walk, or is holding her own thing going, one-year-old can be in bed when take two steps, one-year-old one month left on their own, the proposed time is not a very long walk, and slowly their baby want to walk.

现在说的是一岁后开始走比较合适，书上也有说法是太早走路眼睛易近视，宝宝一岁前都没练习走路，要不就是她自己扶着东西走，一岁时在床上能走两三步，一岁一个月就可以自己走了，建议现在走的时间不要太长，慢慢宝宝自己都想要走路了。

I find it very very easy to be true, I find myself alone when each day's through, Yes I'll admit that I'm a fool for you, Because you're mine, I walk the line Rodney Crowell; All these long years later it's still music to my ears I swear it sounds as good right now as anything I hear I've seen the Mona Lisa I've heard Shakespear read real fine Just like hearing Johnny Cash Sing I Walk The Line Johnny Cash; As sure as night is dark and day is light I keep you on my mind both day and night And happiness I've know proves that it's right Because you're mine, I Walk The Line Because you're mine, I Walk The Line

我发现独自度过每天是如此孤独。是的，我承认你是我无法抗拒的诱惑。因为我是你的，我会一往无前。许多年以后，音乐依然在我的耳边回响。我清楚地知道它听起来就像我听过的所有好音乐那样好听。我见过蒙娜丽莎。我听过莎士比亚诵读真正优美的东西，就像听Johnny Cash唱《一往无前》这首歌。就跟夜晚黑暗白天光明一样，我对你日夜牵挂。你是我的快乐之源。因为我是你的，我会一往无前。因为我是你的，我会一往无前。

It is that one kind can change the electric pulse signal into a electromechanical component of angle displacement or line displacement to walk into the electrical machinery, it is a kind of figure / angle converter that is walked into the control system of the electrical machinery to mainly walk into the controller in fact, power amplifier and walking into the electrical machinery ,etc.

步进电机是一种能将电脉冲信号转换成角位移或线位移的机电元件，实质上是一种数字/角度转换器步进电机控制系统主要由步进控制器，功率放大器及步进电机等组成。

Firstly, the random walk problem is introduced and the solution is schemed out theoretically. And then, the equivalent RC circuit for thermal analysis is discussed as the IC package is divided into many small blocks at the desired level of granularity. The techniques of analyzing the equivalent RC network by random walk algorithm are then discussed. At the same time, an equivalent model for thermal analysis is set up according to an IC chip. The proposed method is then applied to this model for both steady and transient analysis. Two speedup strategies, pseudo parallel walking and walk results reuse, are proposed sequentially to improve the algorithm performance.

首先简述随机行走问题的基本概念，给出问题的理论解法；然后讨论基于三维网格划分的IC芯片热等效阻容网络模型，着重分析随机行走算法在热等效模型中的应用，并建立一个实际芯片的热等效模型，采用随机行走算法模拟计算模型的稳态温度分布和瞬态温度变化；最后针对随机行走算法应用中的不足，提出了伪并行行走和结果重用2种加速技术。

Artist:David Archuleta Song:Crush I hang up the phone tonight Something happened for the first time Deep inside It was a rush, what a rush Cause the possibility that you would ever feel the same way About me It's just too much, just too much Why do I keep running from the truth All I ever think about is you You got me hypnotized, so mesmerized And I just got to know Do you ever think When you're all alone All that we can be Where this thing can go Am I crazy or falling in love Is it really just another crush Do you catch a breath When I look at you 听流行英乐,看美国偶像进行中 Are you holding back Like the way I do Cause I'm tryin, tryin to walk away But I know this crush aint goin away, goin away Has it ever cross you mind When were hangin, spending time girl Are we just friends Is there more, is there more See it's a chance we've gotta take Cause I believe that we can make this into Something that will last, last forever, forever Do you ever think When you're all alone All that we can be Where this thing can go Am I crazy or falling in love Is it really just another crush Do you catch a breath When I look at you Are you holding back Like the way I do Cause I'm tryin, tryin to walk away But I know this crush aint goin away, goin away Why do I keep running from the truth All I ever think about is you You got me hypnotized, so mesmerized And I just got to know Do you ever think When you're all alone All that we can be Where this thing can go Am I crazy or falling in love Is it really just another crush Do you catch a breath When I look at you Are you holding back Like the way I do Cause I'm tryin, tryin to walk away But I know this crush aint goin away, goin away

下面是在Google里翻译出来的，还能凑合着看看吧~~】艺术家：大卫阿丘利塔歌曲：爱恋我挂断电话，今晚一定发生了什么事情，为第一次我内心深处，这是一个繁忙，什么仓促事业的可能性你会感到同样的方式关于我它只是太多了，只是太多为什么我要保持运行，从真相我想到的是你你明白我催眠，所以mesmerized 我只是到了知道你是否想过当您的所有单所有我们能如果这件事可以去我疯了，或堕入爱河是否真的只是另一个爱恋你赶上呼气当我看到你听流行英乐，看美国偶像进行中你是否持有回像我这样做原因我tryin ， tryin走开但我知道这是粉碎走离，走离它以往任何时候都跨你介意当被hangin ，花费时间女童我们只是朋友是有更多的，是有更多的看到它的一个机会，我们已经采取的宝贝事业，我相信我们可以到这一点一些会最后，最后永远，永远你是否想过当您的所有单所有我们能如果这件事可以去我疯了，或堕入爱河是否真的只是另一个爱恋你赶上呼气当我看到你你是否持有回像我这样做原因我tryin ， tryin走开但我知道这是粉碎走离，走离为什么我要保持运行，从真相我想到的是你你明白我催眠，所以mesmerized 我只是到了知道你是否想过当您的所有单所有我们能如果这件事可以去我疯了，或堕入爱河是否真的只是另一个爱恋你赶上呼气当我看到你你是否持有回像我这样做原因我tryin ， tryin走开但我知道这是粉碎走离，走离

I was up all night because I was in pain, she said.

我整夜，因为我在疼痛，她说。

A crow caws /craoks.

乌鸦叫。 51-论文网