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MAIN OUTCOME MEASURES: Proliferation and osteogenic activity of cells were measured by morphology, ultrastructure, and cell proliferation curves, analyzed by calcification nodi Von Kossa staining, and determend by quantitative analysis of contents of cytoplasmic alkaline phosphatase and amendable calcium-cobalt staining.
主要观察指标:通过形态学、超微结构,细胞增殖曲线,钙结节Von kossa法染色以及细胞内碱性磷酸酶定量检测与钙钴法染色确定其增殖与成骨活性。
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Methods: Mesenchymal stem cells were isolated from human term placenta by digestion of collagenase Ⅱ and their unique growth characteristic of attaching to the wall of cell culture flask. The proliferation ability was detected by living cell number counting and propidium iodide staining. Their surface markers were detected by flow cytometry. The cells were induced to osteoblast with dexamethasone, antiscorbutic acid and β-sodium glycerophosphate. And they also were induced to adipocytes with dexamethasone and insulin. After induction, the cells were observed by Von Kossa staining and oil red O staining.
将人足月胎盘组织经胶原酶Ⅱ消化和贴壁培养法获取间充质干细胞,运用活细胞计数和碘化丙吮检测其增殖能力;采用流式细胞术检测其细胞表面标志的表达;用地塞米松、抗坏血酸及β-磷酸甘油诱导其向成骨细胞分化,并用Von Kossa染色进行鉴定;用地塞米松与胰岛素诱导其向脂肪细胞分化,并以油红O染色进行鉴定。
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Ms von Baer says that the Chilean opposition uses Twitter to make up for its poor coverage in the mainstream media.
Ms von Baer说智利的反对党使用微博来弥补其在主流媒体上的弱势。
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As one of the most important contemporary European thinkers, an topping cultural theologian and a classic, Hans Urs von Balthasar (1905-1988) characterized his thought by the deep concern about the possibility of the reconciliation between Christian theology and current western culture and the effort of renewing the tradition of western classical thought, especially the one of biblical and patristic theology, in the context of the contemporary society.
汉斯·厄斯·冯·巴尔塔萨(Hans Urs von Balthasar,1905-1988)是当代欧洲著名的思想家、文化神学家、古典学家,其思想以寻求基督教信仰及其神学与当代西方文化之间的契合为主要关切,注重在当代文化语境中重新阐释、发扬西方古典文化传统、尤其是基督教圣经神学与中世纪教父神学传统,因而在古典主义的气质中透露出深切的时代关怀,在神学的话语中蕴含着丰富的文化意义。
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But I fear Von Croy, digging in the hopes of discovering her alive may only be met with black reality.
但我担心VON CROY他带着LARA依然在生的信念的疯狂挖掘也许只会迎来我们不愿看到的情况。
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Besides, the following two features of the book seem to be very incomprehensible: on the one hand, the book includes a full-scale version of Western astrology, not only medical and meteorological one permitted by the Catholic Church, but even natal astrology forbiden by the chruch as well; on the other hand, at the high time when the Tychonic system of calendrical astronomy constructed by Johann Terrenz Schreck (1576-1630), Johann Adam Schall von Bell (1591–1666) and Giacomo Rho (1593-1638) had been contributed by Schall himself to the newly founded Qing dynasty which had officially adopted it immediately, Smogulecki was preaching in the book a system based on Philippe van Lansberge's (1516 – 1632) heliocentric astronomy, and openly claimed that Schall's Tychonic system still committed a number of deficiencies and was outdated, and therefore should be replaced with the Lanbergian system.
另外,该书的两大特点也令人不解:首先,其中包含有完整版本的西方星占学知识,不仅包括天主教会允许的医学和气象星占,而且还包含受教会禁止的生辰星占;其次,在汤若望(Johann Adam Schall von Bell,字道未,1591–1666)等人介绍到中国来的弟谷天文学系统已经被清朝政府正式采纳,传教士的信誉已经微妙地与这种天文学系统紧密地联系在一起的情况下,穆尼阁居然在书中介绍了以哥白尼"日心地动"说为基础的斯伯格(Philippe van Lansberge, 1516 – 1632)天文学,而且还公然宣称,汤若望引进的系统存在许多不足之处,已经过时,应该用兰斯伯格的天文学加以取代。
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There are also the randomly "discovered" Chinese propaganda banners by Ralo Mayer, the "readymade" objects found by Karel Dudesek, the discarded playing cards collected by Kerstin von Gabain, the travel photographs shot with expired Polaroid film by Lukas Birk and more. These are all works created from the artists' firsthand observations and experiences of everyday people and events in the same environment, China.
还有Ralo Mayer 偶然发现的中文标语横幅,Karel Dudesek 找到的无用的现成物品,Kerstin von Gabain 搜集的被扔掉的纸牌,Lukas Birk用过期的波拉片拍摄的旅游照片等,都是从观察身边的人和事,亲身介入到日常面对的生活当中,以艺术家各自的艺术认识和艺术探索同环境中的现实相契合来创作作品的。
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Rudolf Blasius and Gustav von Hayek developed a grand plan for a multi-nation program on avian migration in Europe, obtained the support of Crown Prince Rudolf of Austria-Hungary, and announced the 1st International Congress of Ornithology in Vienna, April 1884 which was dedicated largely to migration studies.
Rudolf Blasius 和 Gustav von Hayek制定了一个欧洲多国合作研究鸟类迁徙的宏伟计划,并得到了匈牙利-奥地利王储的支持。
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The lower von Mises stress in the calcar region was found at the stovepipe models with a low flare stem (8.78MPa for thick and 8.23MPa for thin cement technique).
在同组模型之股骨calcar 之von Mises 应平均较低(厚骨水8.78MPa 和薄骨水8.23MPa 。
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When Ryback began exploring Hitler's collection, he discovered that a copy of the writings of the Prussian general Carl von Clausewitz was nestled beside a French vegetarian cookbook inscribed to "Monsieur Hitler végétarien."
Ryback 在翻看希特勒的藏书时,他发现一本普鲁士将军 Carl Von Clausewitz 的书紧挨着一本法国素食烹饪书,上书:素食者希特勒先生( Monsieur Hitler végétarien。
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- 推荐网络例句
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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.
曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。
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The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.
稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。
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When I was in school, the rabbi explained everythingin the Bible two different ways.
当我上学的时候,老师解释《圣经》用两种不同的方法。