查询词典 vitro

The effects of FSH and IGF-Ⅰ on apoptosis and production of steroids in granulose cells cultured in vitro.

体外培养颗粒细胞过程中，FSH和IGF-Ⅰ在促进类固醇激素生产，抑制颗粒细胞凋亡中的作用。

ObjectiveTo study the effect and mechanism of menoprogen,on ovary granulose cells' apoptosis from female rats in vitro.

目的 探讨宁心红杞含药血清对离体大鼠卵巢颗粒细胞凋亡的影响及其机制。

There was a spontaneous apoptosis in granulose cells cultured in vitro.

FSH能够促进IGF-Ⅰ的分泌，但是FSH的这些作用能够倍H89阻断。

The appropriate electric fusion parameters for nuclear transfer of porcine somatic cells were investigated using in vitro matured porcine oosytes as recipients and granulose cells as donors in this study.

本研究用体外成熟42~46 h的猪卵母细胞为核移植受体，猪颗粒细胞为核移植供体，对猪体细胞核移植的电融合参数进行了探讨。

OBJECTIVE: To investigate the effects of graphite carbon nanoparticles on cell growth, cycle, and apoptosis in vitro.

目的：观察石墨碳纳米颗粒对体外培养细胞的生长增殖、细胞周期及凋亡的影响。

In order to investigate the mechanisms of pregnancy associated with diabetes harm to gravida and fetal, The Kunming mice models for pregnancy associated with diabetes were simulated, The effects of glucose in different concentrations was detected on the growth of embryo cells in vitro culture.

为研究妊娠合并糖尿病对孕妇及胎儿产生危害的机制，构建妊娠合并糖尿病的昆明小鼠动物模型，检测不同浓度葡萄糖对体外培养胚泡细胞生长的影响。

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

Major developments in the in vitro culture of plants and micropropogation techniques were made by plant scientists at Nottingham, along with the first production of transgenic tomatoes by Professor Don Grierson in the 1980s.

在体外培养的植物和微型繁殖技术上的主要成果，由诺丁汉大学的植物学家取得的，与格雷厄森教授在19世纪80年代研究出的第一代转基因番茄一起。

We dissolve it in guanidine hydrochloride, purify it with Ni++ affinity chromatography column and renature it in vitro.

同时，使用RT-PCR只涉及引物使用与保存，不涉及VSV的使用，可以避免散毒的危险。

The guanosine triphosphate cap at the growing end of microtubules, whose presence is essential to prevent microtubule catastrophes in vitro, has been difficult to observe in vivo.

GTP帽子位于微管的增长末端，它的存在对防治体外微管激变有着至关重要的作用，而这一点在体内却很难观察到。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。